Assessment of field fertility and several in vitro sperm characteristics following the use of different Angus sires in a timed-AI program with suckled Nelore cows
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.livsci.2012.02.018 http://hdl.handle.net/11449/2616 |
Resumo: | In order to provide information that may help researchers to understand the main cause(s) of differences in bull fertility frequently observed in field trials, this study aimed to investigate conception rates as well as several in vitro sperm characteristics of different sires of unknown fertility utilized in a Timed-AI (TAI) program. Suckled Nelore cows submitted to the same TAI protocol were allocated into eight breeding groups of approximately 120 animals each. Frozen semen doses from three Angus bulls and three different batches from each bull were utilized. Approximately 100 doses from each batch were used in TAI. Sires, batches and AI technicians were equally distributed across breeding groups. Cows were examined for pregnancy diagnosis 40 d after TAI. For in vitro sperm analyses, the same thawing procedure was repeated in the laboratory to mimic field conditions. The following in vitro sperm characteristics were assessed: computerized motility, thermal resistance, plasma and acrosomal membrane integrity, lipid peroxidation, morphology, morphometry and chromatin structure. No effect of breeding group, body condition score, AI technician and sire was observed. However, some significant differences among bulls were detected in laboratory analyses. Semen from sire presenting numerically lower (P > 0.05) pregnancy/AI also presented lower (P < 0.05) values in all sperm characteristics analyzed in thermal resistance test at 4 h (Total Motility, Progressive Motility, Average Path Velocity, Straight-Line Velocity, Curvilinear Velocity, Amplitude of Lateral Head Displacement, Beat Cross Frequency, Straightness, Linearity, and Percentage of Rapidly Moving Cells), higher (P < 0.05) Major and Total Defects in sperm morphological test, lower (P < 0.05) Length, Ellipticity and Fourier parameter (Fourier 0) in sperm morphometric analysis as well as higher (P < 0.05) chromatin heterogeneity. It was concluded that, although no bull effect was observed in the field experiment, the sire that presented numerically lower pregnancy/AI also presented lower semen quality according to the laboratory analyses performed. (C) 2012 Elsevier B.V. All rights reserved. |
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Assessment of field fertility and several in vitro sperm characteristics following the use of different Angus sires in a timed-AI program with suckled Nelore cowsBull fertilityConception rateTimed AISperm characteristicsIn order to provide information that may help researchers to understand the main cause(s) of differences in bull fertility frequently observed in field trials, this study aimed to investigate conception rates as well as several in vitro sperm characteristics of different sires of unknown fertility utilized in a Timed-AI (TAI) program. Suckled Nelore cows submitted to the same TAI protocol were allocated into eight breeding groups of approximately 120 animals each. Frozen semen doses from three Angus bulls and three different batches from each bull were utilized. Approximately 100 doses from each batch were used in TAI. Sires, batches and AI technicians were equally distributed across breeding groups. Cows were examined for pregnancy diagnosis 40 d after TAI. For in vitro sperm analyses, the same thawing procedure was repeated in the laboratory to mimic field conditions. The following in vitro sperm characteristics were assessed: computerized motility, thermal resistance, plasma and acrosomal membrane integrity, lipid peroxidation, morphology, morphometry and chromatin structure. No effect of breeding group, body condition score, AI technician and sire was observed. However, some significant differences among bulls were detected in laboratory analyses. Semen from sire presenting numerically lower (P > 0.05) pregnancy/AI also presented lower (P < 0.05) values in all sperm characteristics analyzed in thermal resistance test at 4 h (Total Motility, Progressive Motility, Average Path Velocity, Straight-Line Velocity, Curvilinear Velocity, Amplitude of Lateral Head Displacement, Beat Cross Frequency, Straightness, Linearity, and Percentage of Rapidly Moving Cells), higher (P < 0.05) Major and Total Defects in sperm morphological test, lower (P < 0.05) Length, Ellipticity and Fourier parameter (Fourier 0) in sperm morphometric analysis as well as higher (P < 0.05) chromatin heterogeneity. It was concluded that, although no bull effect was observed in the field experiment, the sire that presented numerically lower pregnancy/AI also presented lower semen quality according to the laboratory analyses performed. (C) 2012 Elsevier B.V. All rights reserved.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)São Paulo State Univ UNESP, Sch Agr Sci & Vet Med, Dept Prevent Vet Med & Anim Reprod, BR-14884900 Jaboticabal, SP, BrazilUniv São Paulo, Sch Vet Med & Anim Sci, Dept Anim Reprod, BR-13630000 Pirassununga, SP, BrazilFed Univ Uberlandia UFU, Sch Vet Med FAMEV, BR-38400902 Uberlandia, MG, BrazilAgropecuaria Fazenda Brasil AFB, BR-78690000 Nova Xavantina, MT, BrazilSão Paulo State Univ UNESP, Sch Agr Sci & Vet Med, Dept Prevent Vet Med & Anim Reprod, BR-14884900 Jaboticabal, SP, BrazilElsevier B.V.Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Universidade Federal de Uberlândia (UFU)Agropecuaria Fazenda Brasil AFBOliveira, Leticia Zoccolaro [UNESP]de Arruda, Rubens PaesCesar de Andrade, Andre FurugenCarvalho Celeghini, Eneiva Carlados Santos, Ricarda MariaBeletti, Marcelo EmilioGuimaraes Peres, Rogerio FonsecaOliveira, Clara Slade [UNESP]Martins Hossepian de Lima, Vera Fernanda [UNESP]2014-05-20T13:15:29Z2014-05-20T13:15:29Z2012-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article38-46application/pdfhttp://dx.doi.org/10.1016/j.livsci.2012.02.018Livestock Science. Amsterdam: Elsevier B.V., v. 146, n. 1, p. 38-46, 2012.1871-1413http://hdl.handle.net/11449/261610.1016/j.livsci.2012.02.018WOS:000303971800006WOS000303971800006.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLivestock Science1.2040,730info:eu-repo/semantics/openAccess2024-06-06T18:10:00Zoai:repositorio.unesp.br:11449/2616Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-06-06T18:10Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Assessment of field fertility and several in vitro sperm characteristics following the use of different Angus sires in a timed-AI program with suckled Nelore cows |
title |
Assessment of field fertility and several in vitro sperm characteristics following the use of different Angus sires in a timed-AI program with suckled Nelore cows |
spellingShingle |
Assessment of field fertility and several in vitro sperm characteristics following the use of different Angus sires in a timed-AI program with suckled Nelore cows Oliveira, Leticia Zoccolaro [UNESP] Bull fertility Conception rate Timed AI Sperm characteristics |
title_short |
Assessment of field fertility and several in vitro sperm characteristics following the use of different Angus sires in a timed-AI program with suckled Nelore cows |
title_full |
Assessment of field fertility and several in vitro sperm characteristics following the use of different Angus sires in a timed-AI program with suckled Nelore cows |
title_fullStr |
Assessment of field fertility and several in vitro sperm characteristics following the use of different Angus sires in a timed-AI program with suckled Nelore cows |
title_full_unstemmed |
Assessment of field fertility and several in vitro sperm characteristics following the use of different Angus sires in a timed-AI program with suckled Nelore cows |
title_sort |
Assessment of field fertility and several in vitro sperm characteristics following the use of different Angus sires in a timed-AI program with suckled Nelore cows |
author |
Oliveira, Leticia Zoccolaro [UNESP] |
author_facet |
Oliveira, Leticia Zoccolaro [UNESP] de Arruda, Rubens Paes Cesar de Andrade, Andre Furugen Carvalho Celeghini, Eneiva Carla dos Santos, Ricarda Maria Beletti, Marcelo Emilio Guimaraes Peres, Rogerio Fonseca Oliveira, Clara Slade [UNESP] Martins Hossepian de Lima, Vera Fernanda [UNESP] |
author_role |
author |
author2 |
de Arruda, Rubens Paes Cesar de Andrade, Andre Furugen Carvalho Celeghini, Eneiva Carla dos Santos, Ricarda Maria Beletti, Marcelo Emilio Guimaraes Peres, Rogerio Fonseca Oliveira, Clara Slade [UNESP] Martins Hossepian de Lima, Vera Fernanda [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) Universidade Federal de Uberlândia (UFU) Agropecuaria Fazenda Brasil AFB |
dc.contributor.author.fl_str_mv |
Oliveira, Leticia Zoccolaro [UNESP] de Arruda, Rubens Paes Cesar de Andrade, Andre Furugen Carvalho Celeghini, Eneiva Carla dos Santos, Ricarda Maria Beletti, Marcelo Emilio Guimaraes Peres, Rogerio Fonseca Oliveira, Clara Slade [UNESP] Martins Hossepian de Lima, Vera Fernanda [UNESP] |
dc.subject.por.fl_str_mv |
Bull fertility Conception rate Timed AI Sperm characteristics |
topic |
Bull fertility Conception rate Timed AI Sperm characteristics |
description |
In order to provide information that may help researchers to understand the main cause(s) of differences in bull fertility frequently observed in field trials, this study aimed to investigate conception rates as well as several in vitro sperm characteristics of different sires of unknown fertility utilized in a Timed-AI (TAI) program. Suckled Nelore cows submitted to the same TAI protocol were allocated into eight breeding groups of approximately 120 animals each. Frozen semen doses from three Angus bulls and three different batches from each bull were utilized. Approximately 100 doses from each batch were used in TAI. Sires, batches and AI technicians were equally distributed across breeding groups. Cows were examined for pregnancy diagnosis 40 d after TAI. For in vitro sperm analyses, the same thawing procedure was repeated in the laboratory to mimic field conditions. The following in vitro sperm characteristics were assessed: computerized motility, thermal resistance, plasma and acrosomal membrane integrity, lipid peroxidation, morphology, morphometry and chromatin structure. No effect of breeding group, body condition score, AI technician and sire was observed. However, some significant differences among bulls were detected in laboratory analyses. Semen from sire presenting numerically lower (P > 0.05) pregnancy/AI also presented lower (P < 0.05) values in all sperm characteristics analyzed in thermal resistance test at 4 h (Total Motility, Progressive Motility, Average Path Velocity, Straight-Line Velocity, Curvilinear Velocity, Amplitude of Lateral Head Displacement, Beat Cross Frequency, Straightness, Linearity, and Percentage of Rapidly Moving Cells), higher (P < 0.05) Major and Total Defects in sperm morphological test, lower (P < 0.05) Length, Ellipticity and Fourier parameter (Fourier 0) in sperm morphometric analysis as well as higher (P < 0.05) chromatin heterogeneity. It was concluded that, although no bull effect was observed in the field experiment, the sire that presented numerically lower pregnancy/AI also presented lower semen quality according to the laboratory analyses performed. (C) 2012 Elsevier B.V. All rights reserved. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-06-01 2014-05-20T13:15:29Z 2014-05-20T13:15:29Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.livsci.2012.02.018 Livestock Science. Amsterdam: Elsevier B.V., v. 146, n. 1, p. 38-46, 2012. 1871-1413 http://hdl.handle.net/11449/2616 10.1016/j.livsci.2012.02.018 WOS:000303971800006 WOS000303971800006.pdf |
url |
http://dx.doi.org/10.1016/j.livsci.2012.02.018 http://hdl.handle.net/11449/2616 |
identifier_str_mv |
Livestock Science. Amsterdam: Elsevier B.V., v. 146, n. 1, p. 38-46, 2012. 1871-1413 10.1016/j.livsci.2012.02.018 WOS:000303971800006 WOS000303971800006.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Livestock Science 1.204 0,730 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
38-46 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803650118776258560 |