Determinação do teor de glicose em bagaço de cana-de-açúcar hidrolizado pelo processo de conversão enzimática

• Autor(a) principal: Rabonato, Aline Cristina
• Data de Publicação: 2015
• Outros Autores: Minhoni, Marli Teixeira de Almeida [UNESP], Cervezan, Thalita Cristina Marques [UNESP], Bonfim, Filipe Pereira Giardini [UNESP], Duarte, Marta Cristina Teixeira
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• DOI: 10.17224/energagric.2015v30n1p11-19
• Texto Completo: http://dx.doi.org/10.17224/energagric.2015v30n1p11-19; http://hdl.handle.net/11449/137613
• topic: Laccase; Manganese peroxidase; Lignin peroxidise; Lacase; Manganês peroxidase; Lignina peroxidase

The production of ethanol and sugar from sugarcane juice generate as byproduct, the bagasse. Currently, the bagasse, an industrial lignocellulosic biomass, can be used for production of second-generation ethanol, since when it is submitted to hydrolytic processes generates fermentable sugars. The objective of this study was to produce fungal enzymes capable of hydrolyzing this lignocellulosic biomass to generate glucose. For this, we used the mushroom species Lentinula edodes, Pleurotus ostreatus, Pleurotus eryngii, and Pycnoporus sanguineus as potential sources of laccase, manganese peroxidase and lignin peroxidase enzymes, capable of hydrolyzing the crushed sugarcane. The hydrolysis process was performed with the highest enzymatic activities observed from laccase in L. edodes (39.23 U-mL after 25 day incubation), P. ostreatus (2.5 U U-mL after 27 day incubation), P. sanguineus (80 U-mL after 27 days of incubation) and P. eryngii (16.45 U-mL 15 days incubation). MnP and LiP showed no significant results. The enzymatic hydrolysis of sugarcane bagasse in natura (32,17% hemicellulose, cellulose 52,45% and 10,62% lignin) and bagasse hydrolyzate with 7,0% H2SO4 (0,20% hemicellulose, 68,82% to 25,33% cellulose and lignin) were evaluated for each enzymatic obtained. Compared to others, the enzymes produced by P. sanguineus incubated in sugarcane bagasse showed better efficiency resulting in glucose with an average content of 0,14 g-L. Although the levels of glucose determined in this work were low in relation to the literature, it can be stated that the laccase, manganese peroxidase and lignin peroxidase enzymes demonstrated good hydrolytic potential, especially those produced by the fungus P. sanguineus.