A nanocarrier system that potentiates the effect of miconazole within different interkingdom biofilms
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1080/20002297.2020.1771071 http://hdl.handle.net/11449/200624 |
Resumo: | Background: Novel and new therapeutic strategies capable of enhancing the efficacy of existing antimicrobials is an attractive proposition to meet the needs of society. Objective: This study aimed to evaluate the potentiating effect of a miconazole (MCZ) nanocarrier system, incorporated with iron oxide nanoparticles (IONPs) and chitosan (CS) (IONPs-CS-MCZ). This was tested on three representative complex interkingdom oral biofilm models (caries, denture and gingivitis). Materials and methods: The planktonic and sessile minimum inhibitory concentrations (MICs) of IONPs-CS-MCZ against different Candida albicans strains were determined, as well as against all represented bacterial species that formed within the three biofilm models. Biofilms were treated for 24 hours with the IONPs-CS nanocarrier system containing MCZ at 64 mg/L, and characterized using a range of bioassays for quantitative and qualitative assessment. Results: MIC results generally showed that IONPs-CS-MCZ was more effective than MCZ alone. IONPs-CS-MCZ also promoted reductions in the number of CFUs, biomass and metabolic activity of the representative biofilms, as well as altering biofilm ultrastructure when compared to untreated biofilms. IONPs-CS-MCZ affected the composition and reduced the CFEs for most of the microorganisms present in the three evaluated biofilms. In particular, the proportion of streptococci in the biofilm composition were reduced in all three models, whilst Fusobacterium spp. percentage reduced in the gingivitis and caries models, respectively. Conclusion: In conclusion, the IONPs-CS-MCZ nanocarrier was efficient against three in vitro models of pathogenic oral biofilms, showing potential to possibly interfere in the synergistic interactions among fungal and bacterial cells within polymicrobial consortia. |
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Repositório Institucional da UNESP |
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A nanocarrier system that potentiates the effect of miconazole within different interkingdom biofilmsantimicrobialsBiofilmsinterkingdommiconazolenanocarriersBackground: Novel and new therapeutic strategies capable of enhancing the efficacy of existing antimicrobials is an attractive proposition to meet the needs of society. Objective: This study aimed to evaluate the potentiating effect of a miconazole (MCZ) nanocarrier system, incorporated with iron oxide nanoparticles (IONPs) and chitosan (CS) (IONPs-CS-MCZ). This was tested on three representative complex interkingdom oral biofilm models (caries, denture and gingivitis). Materials and methods: The planktonic and sessile minimum inhibitory concentrations (MICs) of IONPs-CS-MCZ against different Candida albicans strains were determined, as well as against all represented bacterial species that formed within the three biofilm models. Biofilms were treated for 24 hours with the IONPs-CS nanocarrier system containing MCZ at 64 mg/L, and characterized using a range of bioassays for quantitative and qualitative assessment. Results: MIC results generally showed that IONPs-CS-MCZ was more effective than MCZ alone. IONPs-CS-MCZ also promoted reductions in the number of CFUs, biomass and metabolic activity of the representative biofilms, as well as altering biofilm ultrastructure when compared to untreated biofilms. IONPs-CS-MCZ affected the composition and reduced the CFEs for most of the microorganisms present in the three evaluated biofilms. In particular, the proportion of streptococci in the biofilm composition were reduced in all three models, whilst Fusobacterium spp. percentage reduced in the gingivitis and caries models, respectively. Conclusion: In conclusion, the IONPs-CS-MCZ nanocarrier was efficient against three in vitro models of pathogenic oral biofilms, showing potential to possibly interfere in the synergistic interactions among fungal and bacterial cells within polymicrobial consortia.Department of Preventive and Restorative Dentistry São Paulo State University (Unesp) School of Dentistry AraçatubaOral Sciences Research Group Glasgow Dental School School of Medicine Dentistry and Nursing College of Medical Veterinary and Life Sciences University of GlasgowGraduate Program in Dentistry (GPD - Master's Degree) University of Western São Paulo (UNOESTE)Department of Preventive and Restorative Dentistry São Paulo State University (Unesp) School of Dentistry AraçatubaUniversidade Estadual Paulista (Unesp)University of GlasgowUniversity of Western São Paulo (UNOESTE)Arias, Laís Salomão [UNESP]Brown, Jason LButcher, Mark CDelaney, ChristopherMonteiro, Douglas Roberto [UNESP]Ramage, Gordon2020-12-12T02:11:41Z2020-12-12T02:11:41Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1080/20002297.2020.1771071Journal of Oral Microbiology, v. 12, n. 1, 2020.2000-2297http://hdl.handle.net/11449/20062410.1080/20002297.2020.17710712-s2.0-85086709430Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Oral Microbiologyinfo:eu-repo/semantics/openAccess2021-10-23T14:48:22Zoai:repositorio.unesp.br:11449/200624Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:59:45.239105Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
A nanocarrier system that potentiates the effect of miconazole within different interkingdom biofilms |
title |
A nanocarrier system that potentiates the effect of miconazole within different interkingdom biofilms |
spellingShingle |
A nanocarrier system that potentiates the effect of miconazole within different interkingdom biofilms Arias, Laís Salomão [UNESP] antimicrobials Biofilms interkingdom miconazole nanocarriers |
title_short |
A nanocarrier system that potentiates the effect of miconazole within different interkingdom biofilms |
title_full |
A nanocarrier system that potentiates the effect of miconazole within different interkingdom biofilms |
title_fullStr |
A nanocarrier system that potentiates the effect of miconazole within different interkingdom biofilms |
title_full_unstemmed |
A nanocarrier system that potentiates the effect of miconazole within different interkingdom biofilms |
title_sort |
A nanocarrier system that potentiates the effect of miconazole within different interkingdom biofilms |
author |
Arias, Laís Salomão [UNESP] |
author_facet |
Arias, Laís Salomão [UNESP] Brown, Jason L Butcher, Mark C Delaney, Christopher Monteiro, Douglas Roberto [UNESP] Ramage, Gordon |
author_role |
author |
author2 |
Brown, Jason L Butcher, Mark C Delaney, Christopher Monteiro, Douglas Roberto [UNESP] Ramage, Gordon |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) University of Glasgow University of Western São Paulo (UNOESTE) |
dc.contributor.author.fl_str_mv |
Arias, Laís Salomão [UNESP] Brown, Jason L Butcher, Mark C Delaney, Christopher Monteiro, Douglas Roberto [UNESP] Ramage, Gordon |
dc.subject.por.fl_str_mv |
antimicrobials Biofilms interkingdom miconazole nanocarriers |
topic |
antimicrobials Biofilms interkingdom miconazole nanocarriers |
description |
Background: Novel and new therapeutic strategies capable of enhancing the efficacy of existing antimicrobials is an attractive proposition to meet the needs of society. Objective: This study aimed to evaluate the potentiating effect of a miconazole (MCZ) nanocarrier system, incorporated with iron oxide nanoparticles (IONPs) and chitosan (CS) (IONPs-CS-MCZ). This was tested on three representative complex interkingdom oral biofilm models (caries, denture and gingivitis). Materials and methods: The planktonic and sessile minimum inhibitory concentrations (MICs) of IONPs-CS-MCZ against different Candida albicans strains were determined, as well as against all represented bacterial species that formed within the three biofilm models. Biofilms were treated for 24 hours with the IONPs-CS nanocarrier system containing MCZ at 64 mg/L, and characterized using a range of bioassays for quantitative and qualitative assessment. Results: MIC results generally showed that IONPs-CS-MCZ was more effective than MCZ alone. IONPs-CS-MCZ also promoted reductions in the number of CFUs, biomass and metabolic activity of the representative biofilms, as well as altering biofilm ultrastructure when compared to untreated biofilms. IONPs-CS-MCZ affected the composition and reduced the CFEs for most of the microorganisms present in the three evaluated biofilms. In particular, the proportion of streptococci in the biofilm composition were reduced in all three models, whilst Fusobacterium spp. percentage reduced in the gingivitis and caries models, respectively. Conclusion: In conclusion, the IONPs-CS-MCZ nanocarrier was efficient against three in vitro models of pathogenic oral biofilms, showing potential to possibly interfere in the synergistic interactions among fungal and bacterial cells within polymicrobial consortia. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:11:41Z 2020-12-12T02:11:41Z 2020-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1080/20002297.2020.1771071 Journal of Oral Microbiology, v. 12, n. 1, 2020. 2000-2297 http://hdl.handle.net/11449/200624 10.1080/20002297.2020.1771071 2-s2.0-85086709430 |
url |
http://dx.doi.org/10.1080/20002297.2020.1771071 http://hdl.handle.net/11449/200624 |
identifier_str_mv |
Journal of Oral Microbiology, v. 12, n. 1, 2020. 2000-2297 10.1080/20002297.2020.1771071 2-s2.0-85086709430 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Oral Microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129146843299840 |