Apoptosis during the seasonal spermatogenic cycle of Rana catesbeiana
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1111/j.1469-7580.2006.00582.x http://hdl.handle.net/11449/16560 |
Resumo: | In the bullfrog Rana catesbeiana, testicular weight is constant throughout the year, but the volume densities of germinative and interstitial compartments undergo inverse changes from winter (non-breeding) to summer (breeding). The occurrence of apoptosis in the seminiferous lobules of bullfrogs was investigated in these two periods using sections stained with haematoxylin and eosin (H&E), the TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling) method and transmission electron microscopy. TUNEL-positive cells were observed in the seminiferous lobules, and ultrastructural morphological details confirmed the occurrence of cell death by apoptosis. In summer, the occurrence of several spermatogenic processes (in addition to spermiogenesis and spermiation), and then the overconsumption of Sertoli cell-derived pro-survival factors, could be responsible for the increased density of apoptotic cells. Alternatively, the low apoptotic frequency in winter could be related to the constant homeostasis in the germinative compartment given that most lobules are filled with primary spermatocytes. As volume densities of interstitial and germinative compartments undergo inverse seasonal variations through the year, the incidence of apoptosis (in summer) could play a part in controlling the spermatogenic process, maintaining the lobular size when interstitial tissue is maximally developed. In winter, the low apoptotic cell density leads to spermatogenic recrudescence and, thereby, the production of an adequate quantity of spermatozoa for the next breeding period. Thus, apoptosis may participate not only in the maintenance of spermatogenic homeostasis, but also in the cyclical control of the different spermatogenic processes according to seasonal changes of the testicular compartments as a whole. |
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Apoptosis during the seasonal spermatogenic cycle of Rana catesbeianaamphibiaanuransapoptosiscell deathgerm cellstestisTUNEL methodIn the bullfrog Rana catesbeiana, testicular weight is constant throughout the year, but the volume densities of germinative and interstitial compartments undergo inverse changes from winter (non-breeding) to summer (breeding). The occurrence of apoptosis in the seminiferous lobules of bullfrogs was investigated in these two periods using sections stained with haematoxylin and eosin (H&E), the TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling) method and transmission electron microscopy. TUNEL-positive cells were observed in the seminiferous lobules, and ultrastructural morphological details confirmed the occurrence of cell death by apoptosis. In summer, the occurrence of several spermatogenic processes (in addition to spermiogenesis and spermiation), and then the overconsumption of Sertoli cell-derived pro-survival factors, could be responsible for the increased density of apoptotic cells. Alternatively, the low apoptotic frequency in winter could be related to the constant homeostasis in the germinative compartment given that most lobules are filled with primary spermatocytes. As volume densities of interstitial and germinative compartments undergo inverse seasonal variations through the year, the incidence of apoptosis (in summer) could play a part in controlling the spermatogenic process, maintaining the lobular size when interstitial tissue is maximally developed. In winter, the low apoptotic cell density leads to spermatogenic recrudescence and, thereby, the production of an adequate quantity of spermatozoa for the next breeding period. Thus, apoptosis may participate not only in the maintenance of spermatogenic homeostasis, but also in the cyclical control of the different spermatogenic processes according to seasonal changes of the testicular compartments as a whole.UNESP, Dent Sch, Dept Morphol, BR-14801903 Araraquara, SP, BrazilUNESP, Dent Sch, Dept Morphol, BR-14801903 Araraquara, SP, BrazilBlackwell PublishingUniversidade Estadual Paulista (Unesp)Sasso-Cerri, E.Cerri, Paulo Sérgio [UNESP]Freymuller, E.Miraglia, S. M.2014-05-20T13:46:44Z2014-05-20T13:46:44Z2006-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article21-29application/pdfhttp://dx.doi.org/10.1111/j.1469-7580.2006.00582.xJournal of Anatomy. Oxford: Blackwell Publishing, v. 209, n. 1, p. 21-29, 2006.0021-8782http://hdl.handle.net/11449/1656010.1111/j.1469-7580.2006.00582.xWOS:000238487100003WOS000238487100003.pdf32784959112078820000-0001-5756-5828Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Anatomy2.4791,078info:eu-repo/semantics/openAccess2023-10-03T06:05:20Zoai:repositorio.unesp.br:11449/16560Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-03T06:05:20Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Apoptosis during the seasonal spermatogenic cycle of Rana catesbeiana |
title |
Apoptosis during the seasonal spermatogenic cycle of Rana catesbeiana |
spellingShingle |
Apoptosis during the seasonal spermatogenic cycle of Rana catesbeiana Sasso-Cerri, E. amphibia anurans apoptosis cell death germ cells testis TUNEL method |
title_short |
Apoptosis during the seasonal spermatogenic cycle of Rana catesbeiana |
title_full |
Apoptosis during the seasonal spermatogenic cycle of Rana catesbeiana |
title_fullStr |
Apoptosis during the seasonal spermatogenic cycle of Rana catesbeiana |
title_full_unstemmed |
Apoptosis during the seasonal spermatogenic cycle of Rana catesbeiana |
title_sort |
Apoptosis during the seasonal spermatogenic cycle of Rana catesbeiana |
author |
Sasso-Cerri, E. |
author_facet |
Sasso-Cerri, E. Cerri, Paulo Sérgio [UNESP] Freymuller, E. Miraglia, S. M. |
author_role |
author |
author2 |
Cerri, Paulo Sérgio [UNESP] Freymuller, E. Miraglia, S. M. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Sasso-Cerri, E. Cerri, Paulo Sérgio [UNESP] Freymuller, E. Miraglia, S. M. |
dc.subject.por.fl_str_mv |
amphibia anurans apoptosis cell death germ cells testis TUNEL method |
topic |
amphibia anurans apoptosis cell death germ cells testis TUNEL method |
description |
In the bullfrog Rana catesbeiana, testicular weight is constant throughout the year, but the volume densities of germinative and interstitial compartments undergo inverse changes from winter (non-breeding) to summer (breeding). The occurrence of apoptosis in the seminiferous lobules of bullfrogs was investigated in these two periods using sections stained with haematoxylin and eosin (H&E), the TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling) method and transmission electron microscopy. TUNEL-positive cells were observed in the seminiferous lobules, and ultrastructural morphological details confirmed the occurrence of cell death by apoptosis. In summer, the occurrence of several spermatogenic processes (in addition to spermiogenesis and spermiation), and then the overconsumption of Sertoli cell-derived pro-survival factors, could be responsible for the increased density of apoptotic cells. Alternatively, the low apoptotic frequency in winter could be related to the constant homeostasis in the germinative compartment given that most lobules are filled with primary spermatocytes. As volume densities of interstitial and germinative compartments undergo inverse seasonal variations through the year, the incidence of apoptosis (in summer) could play a part in controlling the spermatogenic process, maintaining the lobular size when interstitial tissue is maximally developed. In winter, the low apoptotic cell density leads to spermatogenic recrudescence and, thereby, the production of an adequate quantity of spermatozoa for the next breeding period. Thus, apoptosis may participate not only in the maintenance of spermatogenic homeostasis, but also in the cyclical control of the different spermatogenic processes according to seasonal changes of the testicular compartments as a whole. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-07-01 2014-05-20T13:46:44Z 2014-05-20T13:46:44Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/j.1469-7580.2006.00582.x Journal of Anatomy. Oxford: Blackwell Publishing, v. 209, n. 1, p. 21-29, 2006. 0021-8782 http://hdl.handle.net/11449/16560 10.1111/j.1469-7580.2006.00582.x WOS:000238487100003 WOS000238487100003.pdf 3278495911207882 0000-0001-5756-5828 |
url |
http://dx.doi.org/10.1111/j.1469-7580.2006.00582.x http://hdl.handle.net/11449/16560 |
identifier_str_mv |
Journal of Anatomy. Oxford: Blackwell Publishing, v. 209, n. 1, p. 21-29, 2006. 0021-8782 10.1111/j.1469-7580.2006.00582.x WOS:000238487100003 WOS000238487100003.pdf 3278495911207882 0000-0001-5756-5828 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Anatomy 2.479 1,078 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
21-29 application/pdf |
dc.publisher.none.fl_str_mv |
Blackwell Publishing |
publisher.none.fl_str_mv |
Blackwell Publishing |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1797789278674092032 |