Bovine Leptospirosis in Caatinga Biome, Brazil: New Insights into Diagnosis and Epidemiology
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/tropicalmed8030177 http://hdl.handle.net/11449/248574 |
Resumo: | Bovine leptospirosis causes economic losses and raises public health concerns. It is possible that there are peculiarities in the epidemiology of leptospirosis in regions with a semiarid climate, such as the Caatinga biome in Brazil, where the climate is hot and dry, and the etiological agent require alternative routes of transmission. This study aimed to close knowledge gaps to the diagnosis and epidemiology of Leptospira spp. infection in cows from the Caatinga biome, Brazil. Samples of the blood, urinary tract (urine, bladder and kidney) and reproductive tract (vaginal fluid, uterus, uterine tube, ovary and placenta) were collected from 42 slaughtered cows. Diagnostic tests included were the microscopic agglutination test (MAT), polymerase chain reaction (PCR) and bacterial isolation. Anti-Leptospira spp. antibodies were found in 27 (64.3%) of the animals analyzed using MAT at a 1:50 dilution (cut-off 50), while 31 (73.8%) animals had at least one organ/fluid where the presence of Leptospira spp. DNA was identified, and 29 animals (69%) were positive at bacteriological culture. The highest sensitivity values for MAT were obtained at the cut-off point of 50. In conclusion, even under hot and dry climate conditions, it is possible that Leptospira spp. can spread through alternative routes such as venereal transmission; moreover, a cut-off of 50 is recommended for the serological diagnosis of cattle from the Caatinga biome. |
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Bovine Leptospirosis in Caatinga Biome, Brazil: New Insights into Diagnosis and Epidemiologybacteriological culturecut-off pointLeptospirasppPCRsemiarid conditionsserologyBovine leptospirosis causes economic losses and raises public health concerns. It is possible that there are peculiarities in the epidemiology of leptospirosis in regions with a semiarid climate, such as the Caatinga biome in Brazil, where the climate is hot and dry, and the etiological agent require alternative routes of transmission. This study aimed to close knowledge gaps to the diagnosis and epidemiology of Leptospira spp. infection in cows from the Caatinga biome, Brazil. Samples of the blood, urinary tract (urine, bladder and kidney) and reproductive tract (vaginal fluid, uterus, uterine tube, ovary and placenta) were collected from 42 slaughtered cows. Diagnostic tests included were the microscopic agglutination test (MAT), polymerase chain reaction (PCR) and bacterial isolation. Anti-Leptospira spp. antibodies were found in 27 (64.3%) of the animals analyzed using MAT at a 1:50 dilution (cut-off 50), while 31 (73.8%) animals had at least one organ/fluid where the presence of Leptospira spp. DNA was identified, and 29 animals (69%) were positive at bacteriological culture. The highest sensitivity values for MAT were obtained at the cut-off point of 50. In conclusion, even under hot and dry climate conditions, it is possible that Leptospira spp. can spread through alternative routes such as venereal transmission; moreover, a cut-off of 50 is recommended for the serological diagnosis of cattle from the Caatinga biome.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Academic Unit of Veterinary Medicine (UAMV) Federal University of Campina Grande (UFCG)Faculty of Veterinary Medicine and Zootechny (FMVZ) University of São Paulo (USP)Institute of Biosciences Department of Microbiology and Immunology University of the São Paulo State (UNESP)Department of Veterinary Science Federal University of Paraíba (UFPB)Institute of Biosciences Department of Microbiology and Immunology University of the São Paulo State (UNESP)CNPq: 302222/2016-2CNPq: 423836/2018-8Federal University of Campina Grande (UFCG)Universidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)Universidade Federal da Paraíba (UFPB)Barnabé, Nathanael Natércio da CostaSoares, Rafael RodriguesBarros, Deivyson Kelvis SilvaNogueira, Denise BatistaCosta, Flávia Teresa Ribeiro daAraújo Júnior, João Pessoa [UNESP]Malossi, Camila Dantas [UNESP]Ullmann, Leila Sabrina [UNESP]Costa, Diego Figueiredo daSilva, Maria Luana Cristiny RodriguesHigino, Severino Silvano dos SantosSantos, Carolina de Sousa Américo BatistaAzevedo, Sérgio Santos deAlves, Clebert José2023-07-29T13:47:48Z2023-07-29T13:47:48Z2023-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/tropicalmed8030177Tropical Medicine and Infectious Disease, v. 8, n. 3, 2023.2414-6366http://hdl.handle.net/11449/24857410.3390/tropicalmed80301772-s2.0-85150939914Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTropical Medicine and Infectious Diseaseinfo:eu-repo/semantics/openAccess2023-07-29T13:47:48Zoai:repositorio.unesp.br:11449/248574Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T13:47:48Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Bovine Leptospirosis in Caatinga Biome, Brazil: New Insights into Diagnosis and Epidemiology |
title |
Bovine Leptospirosis in Caatinga Biome, Brazil: New Insights into Diagnosis and Epidemiology |
spellingShingle |
Bovine Leptospirosis in Caatinga Biome, Brazil: New Insights into Diagnosis and Epidemiology Barnabé, Nathanael Natércio da Costa bacteriological culture cut-off point Leptospiraspp PCR semiarid conditions serology |
title_short |
Bovine Leptospirosis in Caatinga Biome, Brazil: New Insights into Diagnosis and Epidemiology |
title_full |
Bovine Leptospirosis in Caatinga Biome, Brazil: New Insights into Diagnosis and Epidemiology |
title_fullStr |
Bovine Leptospirosis in Caatinga Biome, Brazil: New Insights into Diagnosis and Epidemiology |
title_full_unstemmed |
Bovine Leptospirosis in Caatinga Biome, Brazil: New Insights into Diagnosis and Epidemiology |
title_sort |
Bovine Leptospirosis in Caatinga Biome, Brazil: New Insights into Diagnosis and Epidemiology |
author |
Barnabé, Nathanael Natércio da Costa |
author_facet |
Barnabé, Nathanael Natércio da Costa Soares, Rafael Rodrigues Barros, Deivyson Kelvis Silva Nogueira, Denise Batista Costa, Flávia Teresa Ribeiro da Araújo Júnior, João Pessoa [UNESP] Malossi, Camila Dantas [UNESP] Ullmann, Leila Sabrina [UNESP] Costa, Diego Figueiredo da Silva, Maria Luana Cristiny Rodrigues Higino, Severino Silvano dos Santos Santos, Carolina de Sousa Américo Batista Azevedo, Sérgio Santos de Alves, Clebert José |
author_role |
author |
author2 |
Soares, Rafael Rodrigues Barros, Deivyson Kelvis Silva Nogueira, Denise Batista Costa, Flávia Teresa Ribeiro da Araújo Júnior, João Pessoa [UNESP] Malossi, Camila Dantas [UNESP] Ullmann, Leila Sabrina [UNESP] Costa, Diego Figueiredo da Silva, Maria Luana Cristiny Rodrigues Higino, Severino Silvano dos Santos Santos, Carolina de Sousa Américo Batista Azevedo, Sérgio Santos de Alves, Clebert José |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Federal University of Campina Grande (UFCG) Universidade de São Paulo (USP) Universidade Estadual Paulista (UNESP) Universidade Federal da Paraíba (UFPB) |
dc.contributor.author.fl_str_mv |
Barnabé, Nathanael Natércio da Costa Soares, Rafael Rodrigues Barros, Deivyson Kelvis Silva Nogueira, Denise Batista Costa, Flávia Teresa Ribeiro da Araújo Júnior, João Pessoa [UNESP] Malossi, Camila Dantas [UNESP] Ullmann, Leila Sabrina [UNESP] Costa, Diego Figueiredo da Silva, Maria Luana Cristiny Rodrigues Higino, Severino Silvano dos Santos Santos, Carolina de Sousa Américo Batista Azevedo, Sérgio Santos de Alves, Clebert José |
dc.subject.por.fl_str_mv |
bacteriological culture cut-off point Leptospiraspp PCR semiarid conditions serology |
topic |
bacteriological culture cut-off point Leptospiraspp PCR semiarid conditions serology |
description |
Bovine leptospirosis causes economic losses and raises public health concerns. It is possible that there are peculiarities in the epidemiology of leptospirosis in regions with a semiarid climate, such as the Caatinga biome in Brazil, where the climate is hot and dry, and the etiological agent require alternative routes of transmission. This study aimed to close knowledge gaps to the diagnosis and epidemiology of Leptospira spp. infection in cows from the Caatinga biome, Brazil. Samples of the blood, urinary tract (urine, bladder and kidney) and reproductive tract (vaginal fluid, uterus, uterine tube, ovary and placenta) were collected from 42 slaughtered cows. Diagnostic tests included were the microscopic agglutination test (MAT), polymerase chain reaction (PCR) and bacterial isolation. Anti-Leptospira spp. antibodies were found in 27 (64.3%) of the animals analyzed using MAT at a 1:50 dilution (cut-off 50), while 31 (73.8%) animals had at least one organ/fluid where the presence of Leptospira spp. DNA was identified, and 29 animals (69%) were positive at bacteriological culture. The highest sensitivity values for MAT were obtained at the cut-off point of 50. In conclusion, even under hot and dry climate conditions, it is possible that Leptospira spp. can spread through alternative routes such as venereal transmission; moreover, a cut-off of 50 is recommended for the serological diagnosis of cattle from the Caatinga biome. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T13:47:48Z 2023-07-29T13:47:48Z 2023-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/tropicalmed8030177 Tropical Medicine and Infectious Disease, v. 8, n. 3, 2023. 2414-6366 http://hdl.handle.net/11449/248574 10.3390/tropicalmed8030177 2-s2.0-85150939914 |
url |
http://dx.doi.org/10.3390/tropicalmed8030177 http://hdl.handle.net/11449/248574 |
identifier_str_mv |
Tropical Medicine and Infectious Disease, v. 8, n. 3, 2023. 2414-6366 10.3390/tropicalmed8030177 2-s2.0-85150939914 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Tropical Medicine and Infectious Disease |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1803649554510249984 |