Identificação de microrganismos resistentes ao herbicida ácido 2,4-diclorofenóxiacético (2,4-D) em solos de Rondônia, Brasil
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.redalyc.org/articulo.oa?id=33926990008 http://hdl.handle.net/11449/75332 |
Resumo: | Pesticide application has become very important in controlling pests and other infestations that attack crops and pastures. The study of these organic compounds in the environment and especially the changes they undergo during their incubation periods in soils are still largely unknown, as are their equivalent effects on living organisms. The herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) is widely used to control broadleaf weeds that infest pastures and plantations in the Amazon region. This herbicide is classified toxicologically as Class I; that is, highly toxic. The aim of this study was to isolate and identify microorganisms from soils of the Amazon region, resistant to the toxicity of 2,4-D. For this, aliquots of soil samples were incubated in a mineral medium containing 200mg·l-1 of the compound as sole carbon source. After 18 transfers, the organisms growing in the medium were isolated and identified phylogenetically. The results of the 16S DNA sequencing fragment amplified from strains isolated suggest that Methylobacterium sp., Xanthobacter autotrophicus, Burkholderia cenocepacia, Aurantimonas sp., Campylobacter jejuni and Methylobacterium chloromethanicum, all described in the literature as potential degraders of organic compounds, were present in the soils of the state of Rondônia. These samples were resistant to microbial toxicity of 2,4-D. |
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Identificação de microrganismos resistentes ao herbicida ácido 2,4-diclorofenóxiacético (2,4-D) em solos de Rondônia, BrasilIdentification of microorganisms resistant to the herbicide 2,4-dichlorophenoxyacetic acid (2,4-d) in soils of Rondonia, BrazilPesticide application has become very important in controlling pests and other infestations that attack crops and pastures. The study of these organic compounds in the environment and especially the changes they undergo during their incubation periods in soils are still largely unknown, as are their equivalent effects on living organisms. The herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) is widely used to control broadleaf weeds that infest pastures and plantations in the Amazon region. This herbicide is classified toxicologically as Class I; that is, highly toxic. The aim of this study was to isolate and identify microorganisms from soils of the Amazon region, resistant to the toxicity of 2,4-D. For this, aliquots of soil samples were incubated in a mineral medium containing 200mg·l-1 of the compound as sole carbon source. After 18 transfers, the organisms growing in the medium were isolated and identified phylogenetically. The results of the 16S DNA sequencing fragment amplified from strains isolated suggest that Methylobacterium sp., Xanthobacter autotrophicus, Burkholderia cenocepacia, Aurantimonas sp., Campylobacter jejuni and Methylobacterium chloromethanicum, all described in the literature as potential degraders of organic compounds, were present in the soils of the state of Rondônia. These samples were resistant to microbial toxicity of 2,4-D.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Laboratório de Biogeoquímica Ambiental Wolfgang C. Pfeiffer Universidade Federal de Rondônia (UNIR)Universidade de São PauloEscola de Engenharia de São CarlosInstituto de Ciencias e Tecnologia Universidade Federal de Alfenas (UNIFAL), Rodovia José Aurélio Vilela, No 11.999, CEP: 37715-400 Poços de Caldas, MGUniversidade Estadual de Campinas(UNICAMP)AgResearchUniversidade Estadual Paulista Júlio de Mesquita FilhoCNPqUniversidade Estadual de LondrinaUniversidade Federal de São CarlosUniversidade Federal Do Rio de JaneiroUniversidade Estadual Paulista Júlio de Mesquita FilhoUniversidade Federal de Rondônia (UNIR)Universidade de São Paulo (USP)Escola de Engenharia de São CarlosUniversidade Federal de Alfenas (UNIFAL)Universidade Estadual de Campinas (UNICAMP)AgResearchUniversidade Estadual Paulista (Unesp)Universidade Estadual de Londrina (UEL)Universidade Federal de São Carlos (UFSCar)Universidade Federal do Rio de Janeiro (UFRJ)Higino Mussy, MaríliaBrucha, GuntherGomes Reis, MarizaIkeda Ushimaru, Priscila [UNESP]Yamashita, MiyukiRodrigues Bastos, Wanderley2014-05-27T11:29:05Z2014-05-27T11:29:05Z2013-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article353-357http://www.redalyc.org/articulo.oa?id=33926990008Interciencia, v. 38, n. 5, p. 353-357, 2013.0378-1844http://hdl.handle.net/11449/753322-s2.0-84882803596Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporInterciencia0.2580,183info:eu-repo/semantics/openAccess2021-10-23T10:58:46Zoai:repositorio.unesp.br:11449/75332Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:13:15.195293Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Identificação de microrganismos resistentes ao herbicida ácido 2,4-diclorofenóxiacético (2,4-D) em solos de Rondônia, Brasil Identification of microorganisms resistant to the herbicide 2,4-dichlorophenoxyacetic acid (2,4-d) in soils of Rondonia, Brazil |
title |
Identificação de microrganismos resistentes ao herbicida ácido 2,4-diclorofenóxiacético (2,4-D) em solos de Rondônia, Brasil |
spellingShingle |
Identificação de microrganismos resistentes ao herbicida ácido 2,4-diclorofenóxiacético (2,4-D) em solos de Rondônia, Brasil Higino Mussy, Marília |
title_short |
Identificação de microrganismos resistentes ao herbicida ácido 2,4-diclorofenóxiacético (2,4-D) em solos de Rondônia, Brasil |
title_full |
Identificação de microrganismos resistentes ao herbicida ácido 2,4-diclorofenóxiacético (2,4-D) em solos de Rondônia, Brasil |
title_fullStr |
Identificação de microrganismos resistentes ao herbicida ácido 2,4-diclorofenóxiacético (2,4-D) em solos de Rondônia, Brasil |
title_full_unstemmed |
Identificação de microrganismos resistentes ao herbicida ácido 2,4-diclorofenóxiacético (2,4-D) em solos de Rondônia, Brasil |
title_sort |
Identificação de microrganismos resistentes ao herbicida ácido 2,4-diclorofenóxiacético (2,4-D) em solos de Rondônia, Brasil |
author |
Higino Mussy, Marília |
author_facet |
Higino Mussy, Marília Brucha, Gunther Gomes Reis, Mariza Ikeda Ushimaru, Priscila [UNESP] Yamashita, Miyuki Rodrigues Bastos, Wanderley |
author_role |
author |
author2 |
Brucha, Gunther Gomes Reis, Mariza Ikeda Ushimaru, Priscila [UNESP] Yamashita, Miyuki Rodrigues Bastos, Wanderley |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Rondônia (UNIR) Universidade de São Paulo (USP) Escola de Engenharia de São Carlos Universidade Federal de Alfenas (UNIFAL) Universidade Estadual de Campinas (UNICAMP) AgResearch Universidade Estadual Paulista (Unesp) Universidade Estadual de Londrina (UEL) Universidade Federal de São Carlos (UFSCar) Universidade Federal do Rio de Janeiro (UFRJ) |
dc.contributor.author.fl_str_mv |
Higino Mussy, Marília Brucha, Gunther Gomes Reis, Mariza Ikeda Ushimaru, Priscila [UNESP] Yamashita, Miyuki Rodrigues Bastos, Wanderley |
description |
Pesticide application has become very important in controlling pests and other infestations that attack crops and pastures. The study of these organic compounds in the environment and especially the changes they undergo during their incubation periods in soils are still largely unknown, as are their equivalent effects on living organisms. The herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) is widely used to control broadleaf weeds that infest pastures and plantations in the Amazon region. This herbicide is classified toxicologically as Class I; that is, highly toxic. The aim of this study was to isolate and identify microorganisms from soils of the Amazon region, resistant to the toxicity of 2,4-D. For this, aliquots of soil samples were incubated in a mineral medium containing 200mg·l-1 of the compound as sole carbon source. After 18 transfers, the organisms growing in the medium were isolated and identified phylogenetically. The results of the 16S DNA sequencing fragment amplified from strains isolated suggest that Methylobacterium sp., Xanthobacter autotrophicus, Burkholderia cenocepacia, Aurantimonas sp., Campylobacter jejuni and Methylobacterium chloromethanicum, all described in the literature as potential degraders of organic compounds, were present in the soils of the state of Rondônia. These samples were resistant to microbial toxicity of 2,4-D. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-05-01 2014-05-27T11:29:05Z 2014-05-27T11:29:05Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.redalyc.org/articulo.oa?id=33926990008 Interciencia, v. 38, n. 5, p. 353-357, 2013. 0378-1844 http://hdl.handle.net/11449/75332 2-s2.0-84882803596 |
url |
http://www.redalyc.org/articulo.oa?id=33926990008 http://hdl.handle.net/11449/75332 |
identifier_str_mv |
Interciencia, v. 38, n. 5, p. 353-357, 2013. 0378-1844 2-s2.0-84882803596 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Interciencia 0.258 0,183 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
353-357 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129174023438336 |