Determinação da taxa de respiração em sementes de soja pelo método da titulação

• Autor(a) principal: Crispim, J. E.
• Data de Publicação: 1994
• Outros Autores: Martins, J. C., Pires, J. C., Antonio, C., Cavariani, REC
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://seer.sct.embrapa.br/index.php/pab/article/view/4203; http://hdl.handle.net/11449/36637
• topic: SEED QUALITY; CO2 FIXING; GERMINATION; GERMINATOR; VIGOR

This trial was carried out under laboratory conditions, with three lots of Iguacu soybean seeds, to determine the respiration rate by the titulation method. A randomized complete block design was used, with four repetitions, each one of them with 50 seeds and a control without seeds for each lot. The seeds were placed in gerbox with 40 mi of KOH 0.1N, on distilled water wet blotting paper for fixing the CO2 produced by seeds respiration. The material was placed in germinator at constant 25 degrees C for 16, 24 and 48 hours. After these periods, the titulation of the fixative solution was performed with HCl 0.1N to check the respiration rate. The most deteriorated seeds were those that presented the highest respiration rates, which were measured in mg of CO2/g of dry matter, when compared with the most vigorous lots. The titulation method was suitable for the evaluation of soybean seed respiration rate because the final results separated the lots in three different quality levels that were compatible with the results obtained from the accelerated aging and emergence.