Effectiveness in the Removal of Endotoxins and Microbiological Profile in Primary Endodontic Infections Using 3 Different Instrumentation Systems: A Randomized Clinical Study
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.joen.2017.03.032 http://hdl.handle.net/11449/174734 |
Resumo: | Introduction This clinical study was conducted to correlate the microbiological profile and levels of endotoxins found in primary endodontic infection with the presence of clinical features and to evaluate the removal of microorganisms and endotoxins using rotary, reciprocating, and hybrid systems for biomechanical preparation. Methods Thirty single root canals with primary endodontic infection were evaluated with signs and symptoms and were randomly divided into 3 groups according to the instrumentation system used (n = 10) as follows: rotary Mtwo instruments (VDW, Munich, Germany) with 8 files, the reciprocating Reciproc system (VDW) with a single file, and Genius hybrid instruments with 3 files (1 rotary and 2 reciprocating files) with irrigation using 24 mL 2.5% sodium hypochlorite. Samples were collected before (S1) and after instrumentation (S2) before being submitted to microbiological culture (colony-forming units/mL) and the checkerboard DNA-DNA hybridization test. Endotoxins were quantified using the limulus amebocyte lysate assay. Results Microbiological culture showed statistical differences in the reduction of colony-forming units/mL with all systems tested (P <.05), but no statistical difference was found among the groups. The most frequently detected species were Capnocytophaga ochracea (53%) and Fusobacterium nucleatum (53%) at S1 and F. nucleatum (50%) and Leptotrichia buccalis (50%) at S2. As for the reduction of endotoxins at S2, Mtwo presented the best results (95.05%) followed by the Genius (91.85%) and Reciproc (64.68%) groups, but no statistical difference was found among the groups. Previous pain, tenderness to percussion, and presence of a sinus tract were associated with specific microorganisms (P <.05). Conclusions Signs and symptoms were correlated with microorganisms. Endodontic treatment was effective in reducing bacteria and endotoxins but was not capable of completely removing them from the root canal. |
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Effectiveness in the Removal of Endotoxins and Microbiological Profile in Primary Endodontic Infections Using 3 Different Instrumentation Systems: A Randomized Clinical StudyCheckerboard DNA-DNA hybridizationinstrumentationprimary endodontic infectionIntroduction This clinical study was conducted to correlate the microbiological profile and levels of endotoxins found in primary endodontic infection with the presence of clinical features and to evaluate the removal of microorganisms and endotoxins using rotary, reciprocating, and hybrid systems for biomechanical preparation. Methods Thirty single root canals with primary endodontic infection were evaluated with signs and symptoms and were randomly divided into 3 groups according to the instrumentation system used (n = 10) as follows: rotary Mtwo instruments (VDW, Munich, Germany) with 8 files, the reciprocating Reciproc system (VDW) with a single file, and Genius hybrid instruments with 3 files (1 rotary and 2 reciprocating files) with irrigation using 24 mL 2.5% sodium hypochlorite. Samples were collected before (S1) and after instrumentation (S2) before being submitted to microbiological culture (colony-forming units/mL) and the checkerboard DNA-DNA hybridization test. Endotoxins were quantified using the limulus amebocyte lysate assay. Results Microbiological culture showed statistical differences in the reduction of colony-forming units/mL with all systems tested (P <.05), but no statistical difference was found among the groups. The most frequently detected species were Capnocytophaga ochracea (53%) and Fusobacterium nucleatum (53%) at S1 and F. nucleatum (50%) and Leptotrichia buccalis (50%) at S2. As for the reduction of endotoxins at S2, Mtwo presented the best results (95.05%) followed by the Genius (91.85%) and Reciproc (64.68%) groups, but no statistical difference was found among the groups. Previous pain, tenderness to percussion, and presence of a sinus tract were associated with specific microorganisms (P <.05). Conclusions Signs and symptoms were correlated with microorganisms. Endodontic treatment was effective in reducing bacteria and endotoxins but was not capable of completely removing them from the root canal.Department of Restorative Dentistry Endodontic Division São Paulo State University (UNESP) Institute of Science and Technology São José dos CamposSuperior School of Agriculture “Luiz de Queiroz” Technical Section of Information Technology University of São PauloDepartment of Restorative Dentistry Endodontic Division São Paulo State University (UNESP) Institute of Science and Technology São José dos CamposUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Cavalli, Daiana [UNESP]Toia, Cássia Cestari [UNESP]Flores Orozco, Esteban Isai [UNESP]Khoury, Rayana Duarte [UNESP]Cardoso, Flávia Goulart da Rosa [UNESP]Alves, Marcelo CorrêaCarvalho, Cláudio Antônio Talge [UNESP]Valera, Marcia Carneiro [UNESP]2018-12-11T17:12:37Z2018-12-11T17:12:37Z2017-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1237-1245application/pdfhttp://dx.doi.org/10.1016/j.joen.2017.03.032Journal of Endodontics, v. 43, n. 8, p. 1237-1245, 2017.0099-2399http://hdl.handle.net/11449/17473410.1016/j.joen.2017.03.0322-s2.0-850204143362-s2.0-85020414336.pdf93046001665831000000-0003-0987-5594Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Endodontics1,585info:eu-repo/semantics/openAccess2023-12-03T06:17:28Zoai:repositorio.unesp.br:11449/174734Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:25:24.876374Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effectiveness in the Removal of Endotoxins and Microbiological Profile in Primary Endodontic Infections Using 3 Different Instrumentation Systems: A Randomized Clinical Study |
title |
Effectiveness in the Removal of Endotoxins and Microbiological Profile in Primary Endodontic Infections Using 3 Different Instrumentation Systems: A Randomized Clinical Study |
spellingShingle |
Effectiveness in the Removal of Endotoxins and Microbiological Profile in Primary Endodontic Infections Using 3 Different Instrumentation Systems: A Randomized Clinical Study Cavalli, Daiana [UNESP] Checkerboard DNA-DNA hybridization instrumentation primary endodontic infection |
title_short |
Effectiveness in the Removal of Endotoxins and Microbiological Profile in Primary Endodontic Infections Using 3 Different Instrumentation Systems: A Randomized Clinical Study |
title_full |
Effectiveness in the Removal of Endotoxins and Microbiological Profile in Primary Endodontic Infections Using 3 Different Instrumentation Systems: A Randomized Clinical Study |
title_fullStr |
Effectiveness in the Removal of Endotoxins and Microbiological Profile in Primary Endodontic Infections Using 3 Different Instrumentation Systems: A Randomized Clinical Study |
title_full_unstemmed |
Effectiveness in the Removal of Endotoxins and Microbiological Profile in Primary Endodontic Infections Using 3 Different Instrumentation Systems: A Randomized Clinical Study |
title_sort |
Effectiveness in the Removal of Endotoxins and Microbiological Profile in Primary Endodontic Infections Using 3 Different Instrumentation Systems: A Randomized Clinical Study |
author |
Cavalli, Daiana [UNESP] |
author_facet |
Cavalli, Daiana [UNESP] Toia, Cássia Cestari [UNESP] Flores Orozco, Esteban Isai [UNESP] Khoury, Rayana Duarte [UNESP] Cardoso, Flávia Goulart da Rosa [UNESP] Alves, Marcelo Corrêa Carvalho, Cláudio Antônio Talge [UNESP] Valera, Marcia Carneiro [UNESP] |
author_role |
author |
author2 |
Toia, Cássia Cestari [UNESP] Flores Orozco, Esteban Isai [UNESP] Khoury, Rayana Duarte [UNESP] Cardoso, Flávia Goulart da Rosa [UNESP] Alves, Marcelo Corrêa Carvalho, Cláudio Antônio Talge [UNESP] Valera, Marcia Carneiro [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Cavalli, Daiana [UNESP] Toia, Cássia Cestari [UNESP] Flores Orozco, Esteban Isai [UNESP] Khoury, Rayana Duarte [UNESP] Cardoso, Flávia Goulart da Rosa [UNESP] Alves, Marcelo Corrêa Carvalho, Cláudio Antônio Talge [UNESP] Valera, Marcia Carneiro [UNESP] |
dc.subject.por.fl_str_mv |
Checkerboard DNA-DNA hybridization instrumentation primary endodontic infection |
topic |
Checkerboard DNA-DNA hybridization instrumentation primary endodontic infection |
description |
Introduction This clinical study was conducted to correlate the microbiological profile and levels of endotoxins found in primary endodontic infection with the presence of clinical features and to evaluate the removal of microorganisms and endotoxins using rotary, reciprocating, and hybrid systems for biomechanical preparation. Methods Thirty single root canals with primary endodontic infection were evaluated with signs and symptoms and were randomly divided into 3 groups according to the instrumentation system used (n = 10) as follows: rotary Mtwo instruments (VDW, Munich, Germany) with 8 files, the reciprocating Reciproc system (VDW) with a single file, and Genius hybrid instruments with 3 files (1 rotary and 2 reciprocating files) with irrigation using 24 mL 2.5% sodium hypochlorite. Samples were collected before (S1) and after instrumentation (S2) before being submitted to microbiological culture (colony-forming units/mL) and the checkerboard DNA-DNA hybridization test. Endotoxins were quantified using the limulus amebocyte lysate assay. Results Microbiological culture showed statistical differences in the reduction of colony-forming units/mL with all systems tested (P <.05), but no statistical difference was found among the groups. The most frequently detected species were Capnocytophaga ochracea (53%) and Fusobacterium nucleatum (53%) at S1 and F. nucleatum (50%) and Leptotrichia buccalis (50%) at S2. As for the reduction of endotoxins at S2, Mtwo presented the best results (95.05%) followed by the Genius (91.85%) and Reciproc (64.68%) groups, but no statistical difference was found among the groups. Previous pain, tenderness to percussion, and presence of a sinus tract were associated with specific microorganisms (P <.05). Conclusions Signs and symptoms were correlated with microorganisms. Endodontic treatment was effective in reducing bacteria and endotoxins but was not capable of completely removing them from the root canal. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-08-01 2018-12-11T17:12:37Z 2018-12-11T17:12:37Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.joen.2017.03.032 Journal of Endodontics, v. 43, n. 8, p. 1237-1245, 2017. 0099-2399 http://hdl.handle.net/11449/174734 10.1016/j.joen.2017.03.032 2-s2.0-85020414336 2-s2.0-85020414336.pdf 9304600166583100 0000-0003-0987-5594 |
url |
http://dx.doi.org/10.1016/j.joen.2017.03.032 http://hdl.handle.net/11449/174734 |
identifier_str_mv |
Journal of Endodontics, v. 43, n. 8, p. 1237-1245, 2017. 0099-2399 10.1016/j.joen.2017.03.032 2-s2.0-85020414336 2-s2.0-85020414336.pdf 9304600166583100 0000-0003-0987-5594 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Endodontics 1,585 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1237-1245 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129065118334976 |