The influence of adrenergic blockade in rats with apical periodontitis under chronic stress conditions

Detalhes bibliográficos
Autor(a) principal: Khoury, Rayana Duarte [UNESP]
Data de Publicação: 2020
Outros Autores: Prado, Renata Falchete do [UNESP], Matos, Felipe de Souza [UNESP], Meireles, Bruna Ribas de [UNESP], Cardoso, Flávia Goulart da Rosa [UNESP], Oliveira, Luciane Dias de [UNESP], Carvalho, Cláudio Antonio Talge [UNESP], Valera, Marcia Carneiro [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.archoralbio.2019.104590
http://hdl.handle.net/11449/201326
Resumo: Objective: To investigate the influence of chronic stress and adrenergic blockade in a rat model of apical periodontitis. Methods: Thirty-two Wistar rats were submitted to an animal model of periapical lesion and randomly divided into 4 groups (n = 8): no stress (NS); stress + saline solution (SS); stress + β-adrenergic blocker (Sβ); stress + α-adrenergic blocker (Sα). The SS, Sβ and Sα groups were submitted to an animal model of chronic stress for 28 days and received daily injections of saline solution, propranolol (β adrenergic blocker) and phentolamine (α adrenergic blocker), respectively. After 28 days the animals were euthanized and the following analyses were carried out: a) serum corticosterone levels through Radioimmunoassay; b) measurement of serum levels of IL-1B, IL-6, IL-10 and IL-17 by enzyme-linked immunosorbent assay (ELISA); c) volume of periapical bone resorption by micro-computed tomography; d) histomorphometric analysis by staining with hematoxylin and eosin; e) expression of β-AR, α-AR, receptor activator of nuclear factor kappa-B ligand (RANKL) and osteoprotegerin (OPG) by immunohistochemistry; f) tartrate-resistant acid phosphatase (TRAP) staining; g) ex-vivo cytokine release followed by the stimulation with LPS in superfusion system, by ELISA. Results: SS group displayed significantly higher corticosterone levels than NS group (non-stressed). Higher IL-1β serum level was observed in the NS group (p <.05); compared to all stressed groups. Other cytokines were present in similar amounts in the serum of all groups. All groups presented similar periapical lesions. All groups presented moderate inflammatory infiltrate, without statistically significant differences between them. No differences were observed regarding β-AR, α-AR, Rank-L and OPG expression. The number of TRAP-positive cells was significantly decreased in the groups that received daily injections of adrenergic blockers. The IL-1β release followed LPS stimulation was significantly suppressed when the superfusion media contained propranolol (p <.05). Perfusion containing phentolamine induced a greater release of IL-10. TGF-β was significantly suppressed by phentolamine perfusion in the NS group (p <.05). Conclusions: Chronic stress can significantly change the inflammatory cytokines release. Rank-L/OPG system and periapical lesion volume were not affected following the current method applied. The administration of adrenergic blockers was not able to modulate the inflammatory response but presented effectivity in reducing the number of osteoclasts in the periapical region.
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spelling The influence of adrenergic blockade in rats with apical periodontitis under chronic stress conditionsAdrenergic blockersApical periodontitisChronic stressPhentolaminePropranololObjective: To investigate the influence of chronic stress and adrenergic blockade in a rat model of apical periodontitis. Methods: Thirty-two Wistar rats were submitted to an animal model of periapical lesion and randomly divided into 4 groups (n = 8): no stress (NS); stress + saline solution (SS); stress + β-adrenergic blocker (Sβ); stress + α-adrenergic blocker (Sα). The SS, Sβ and Sα groups were submitted to an animal model of chronic stress for 28 days and received daily injections of saline solution, propranolol (β adrenergic blocker) and phentolamine (α adrenergic blocker), respectively. After 28 days the animals were euthanized and the following analyses were carried out: a) serum corticosterone levels through Radioimmunoassay; b) measurement of serum levels of IL-1B, IL-6, IL-10 and IL-17 by enzyme-linked immunosorbent assay (ELISA); c) volume of periapical bone resorption by micro-computed tomography; d) histomorphometric analysis by staining with hematoxylin and eosin; e) expression of β-AR, α-AR, receptor activator of nuclear factor kappa-B ligand (RANKL) and osteoprotegerin (OPG) by immunohistochemistry; f) tartrate-resistant acid phosphatase (TRAP) staining; g) ex-vivo cytokine release followed by the stimulation with LPS in superfusion system, by ELISA. Results: SS group displayed significantly higher corticosterone levels than NS group (non-stressed). Higher IL-1β serum level was observed in the NS group (p <.05); compared to all stressed groups. Other cytokines were present in similar amounts in the serum of all groups. All groups presented similar periapical lesions. All groups presented moderate inflammatory infiltrate, without statistically significant differences between them. No differences were observed regarding β-AR, α-AR, Rank-L and OPG expression. The number of TRAP-positive cells was significantly decreased in the groups that received daily injections of adrenergic blockers. The IL-1β release followed LPS stimulation was significantly suppressed when the superfusion media contained propranolol (p <.05). Perfusion containing phentolamine induced a greater release of IL-10. TGF-β was significantly suppressed by phentolamine perfusion in the NS group (p <.05). Conclusions: Chronic stress can significantly change the inflammatory cytokines release. Rank-L/OPG system and periapical lesion volume were not affected following the current method applied. The administration of adrenergic blockers was not able to modulate the inflammatory response but presented effectivity in reducing the number of osteoclasts in the periapical region.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Restorative Dentistry Institute of Science and Technology São Paulo State University (Unesp)Department of Restorative Dentistry Institute of Science and Technology São Paulo State University (Unesp)FAPESP: 2016/04711-7FAPESP: 2016/05821-0Universidade Estadual Paulista (Unesp)Khoury, Rayana Duarte [UNESP]Prado, Renata Falchete do [UNESP]Matos, Felipe de Souza [UNESP]Meireles, Bruna Ribas de [UNESP]Cardoso, Flávia Goulart da Rosa [UNESP]Oliveira, Luciane Dias de [UNESP]Carvalho, Cláudio Antonio Talge [UNESP]Valera, Marcia Carneiro [UNESP]2020-12-12T02:29:44Z2020-12-12T02:29:44Z2020-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.archoralbio.2019.104590Archives of Oral Biology, v. 110.1879-15060003-9969http://hdl.handle.net/11449/20132610.1016/j.archoralbio.2019.1045902-s2.0-8507491147493046001665831000000-0003-0987-5594Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Oral Biologyinfo:eu-repo/semantics/openAccess2021-12-14T10:54:37Zoai:repositorio.unesp.br:11449/201326Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:03:58.801940Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv The influence of adrenergic blockade in rats with apical periodontitis under chronic stress conditions
title The influence of adrenergic blockade in rats with apical periodontitis under chronic stress conditions
spellingShingle The influence of adrenergic blockade in rats with apical periodontitis under chronic stress conditions
Khoury, Rayana Duarte [UNESP]
Adrenergic blockers
Apical periodontitis
Chronic stress
Phentolamine
Propranolol
title_short The influence of adrenergic blockade in rats with apical periodontitis under chronic stress conditions
title_full The influence of adrenergic blockade in rats with apical periodontitis under chronic stress conditions
title_fullStr The influence of adrenergic blockade in rats with apical periodontitis under chronic stress conditions
title_full_unstemmed The influence of adrenergic blockade in rats with apical periodontitis under chronic stress conditions
title_sort The influence of adrenergic blockade in rats with apical periodontitis under chronic stress conditions
author Khoury, Rayana Duarte [UNESP]
author_facet Khoury, Rayana Duarte [UNESP]
Prado, Renata Falchete do [UNESP]
Matos, Felipe de Souza [UNESP]
Meireles, Bruna Ribas de [UNESP]
Cardoso, Flávia Goulart da Rosa [UNESP]
Oliveira, Luciane Dias de [UNESP]
Carvalho, Cláudio Antonio Talge [UNESP]
Valera, Marcia Carneiro [UNESP]
author_role author
author2 Prado, Renata Falchete do [UNESP]
Matos, Felipe de Souza [UNESP]
Meireles, Bruna Ribas de [UNESP]
Cardoso, Flávia Goulart da Rosa [UNESP]
Oliveira, Luciane Dias de [UNESP]
Carvalho, Cláudio Antonio Talge [UNESP]
Valera, Marcia Carneiro [UNESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Khoury, Rayana Duarte [UNESP]
Prado, Renata Falchete do [UNESP]
Matos, Felipe de Souza [UNESP]
Meireles, Bruna Ribas de [UNESP]
Cardoso, Flávia Goulart da Rosa [UNESP]
Oliveira, Luciane Dias de [UNESP]
Carvalho, Cláudio Antonio Talge [UNESP]
Valera, Marcia Carneiro [UNESP]
dc.subject.por.fl_str_mv Adrenergic blockers
Apical periodontitis
Chronic stress
Phentolamine
Propranolol
topic Adrenergic blockers
Apical periodontitis
Chronic stress
Phentolamine
Propranolol
description Objective: To investigate the influence of chronic stress and adrenergic blockade in a rat model of apical periodontitis. Methods: Thirty-two Wistar rats were submitted to an animal model of periapical lesion and randomly divided into 4 groups (n = 8): no stress (NS); stress + saline solution (SS); stress + β-adrenergic blocker (Sβ); stress + α-adrenergic blocker (Sα). The SS, Sβ and Sα groups were submitted to an animal model of chronic stress for 28 days and received daily injections of saline solution, propranolol (β adrenergic blocker) and phentolamine (α adrenergic blocker), respectively. After 28 days the animals were euthanized and the following analyses were carried out: a) serum corticosterone levels through Radioimmunoassay; b) measurement of serum levels of IL-1B, IL-6, IL-10 and IL-17 by enzyme-linked immunosorbent assay (ELISA); c) volume of periapical bone resorption by micro-computed tomography; d) histomorphometric analysis by staining with hematoxylin and eosin; e) expression of β-AR, α-AR, receptor activator of nuclear factor kappa-B ligand (RANKL) and osteoprotegerin (OPG) by immunohistochemistry; f) tartrate-resistant acid phosphatase (TRAP) staining; g) ex-vivo cytokine release followed by the stimulation with LPS in superfusion system, by ELISA. Results: SS group displayed significantly higher corticosterone levels than NS group (non-stressed). Higher IL-1β serum level was observed in the NS group (p <.05); compared to all stressed groups. Other cytokines were present in similar amounts in the serum of all groups. All groups presented similar periapical lesions. All groups presented moderate inflammatory infiltrate, without statistically significant differences between them. No differences were observed regarding β-AR, α-AR, Rank-L and OPG expression. The number of TRAP-positive cells was significantly decreased in the groups that received daily injections of adrenergic blockers. The IL-1β release followed LPS stimulation was significantly suppressed when the superfusion media contained propranolol (p <.05). Perfusion containing phentolamine induced a greater release of IL-10. TGF-β was significantly suppressed by phentolamine perfusion in the NS group (p <.05). Conclusions: Chronic stress can significantly change the inflammatory cytokines release. Rank-L/OPG system and periapical lesion volume were not affected following the current method applied. The administration of adrenergic blockers was not able to modulate the inflammatory response but presented effectivity in reducing the number of osteoclasts in the periapical region.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T02:29:44Z
2020-12-12T02:29:44Z
2020-02-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.archoralbio.2019.104590
Archives of Oral Biology, v. 110.
1879-1506
0003-9969
http://hdl.handle.net/11449/201326
10.1016/j.archoralbio.2019.104590
2-s2.0-85074911474
9304600166583100
0000-0003-0987-5594
url http://dx.doi.org/10.1016/j.archoralbio.2019.104590
http://hdl.handle.net/11449/201326
identifier_str_mv Archives of Oral Biology, v. 110.
1879-1506
0003-9969
10.1016/j.archoralbio.2019.104590
2-s2.0-85074911474
9304600166583100
0000-0003-0987-5594
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Archives of Oral Biology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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