The Combination of Fasting, Acute Resistance Exercise, and Protein Ingestion Led to Different Responses of Autophagy Markers in Gastrocnemius and Liver Samples
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/nu12030641 http://hdl.handle.net/11449/195367 |
Resumo: | The present study verified the responses of proteins related to the autophagy pathway after 10 h of fast with resistance exercise and protein ingestion in skeletal muscle and liver samples. The rats were distributed into five experimental groups: control (CT; sedentary and without gavage after fast), exercise immediately (EXE-imm; after fast, rats were submitted to the resistance protocol and received water by gavage immediately after exercise), exercise after 1 h (EXE-1h; after fast, rats were submitted to the resistance protocol and received water by gavage 1 h after exercise), exercise and supplementation immediately after exercise (EXE/Suppl-imm; after fast, rats were submitted to the resistance protocol and received a mix of casein: whey protein 1:1 (w/w) by gavage immediately after exercise), exercise and supplementation 1 h after exercise (EXE/Suppl-1h; after fast, rats were submitted to the resistance protocol and received a mix of casein: whey protein 1:1 (w/w) by gavage 1 h after exercise). In summary, the current findings show that the combination of fasting, acute resistance exercise, and protein blend ingestion (immediately or 1 h after the exercise stimulus) increased the serum levels of leucine, insulin, and glucose, as well as the autophagy protein contents in skeletal muscle, but decreased other proteins related to the autophagic pathway in the liver. These results deserve further mechanistic investigations since athletes are combining fasting with physical exercise to enhance health and performance outcomes. |
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The Combination of Fasting, Acute Resistance Exercise, and Protein Ingestion Led to Different Responses of Autophagy Markers in Gastrocnemius and Liver Samplesresistance exerciseautophagyleucinemammalian target of rapamycin (mTOR)livermuscleThe present study verified the responses of proteins related to the autophagy pathway after 10 h of fast with resistance exercise and protein ingestion in skeletal muscle and liver samples. The rats were distributed into five experimental groups: control (CT; sedentary and without gavage after fast), exercise immediately (EXE-imm; after fast, rats were submitted to the resistance protocol and received water by gavage immediately after exercise), exercise after 1 h (EXE-1h; after fast, rats were submitted to the resistance protocol and received water by gavage 1 h after exercise), exercise and supplementation immediately after exercise (EXE/Suppl-imm; after fast, rats were submitted to the resistance protocol and received a mix of casein: whey protein 1:1 (w/w) by gavage immediately after exercise), exercise and supplementation 1 h after exercise (EXE/Suppl-1h; after fast, rats were submitted to the resistance protocol and received a mix of casein: whey protein 1:1 (w/w) by gavage 1 h after exercise). In summary, the current findings show that the combination of fasting, acute resistance exercise, and protein blend ingestion (immediately or 1 h after the exercise stimulus) increased the serum levels of leucine, insulin, and glucose, as well as the autophagy protein contents in skeletal muscle, but decreased other proteins related to the autophagic pathway in the liver. These results deserve further mechanistic investigations since athletes are combining fasting with physical exercise to enhance health and performance outcomes.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Sao Paulo, Ribeirao Preto Med Sch, Postgrad Program Rehabil & Funct Performance, BR-14049900 Ribeirao Preto, SP, BrazilState Univ Sao Paulo Julio de Mesquita Filho Arar, Postgrad Program Nutr Sci, BR-14800903 Araraquara, SP, BrazilUniv Sao Paulo, Sch Phys Educ & Sport Ribeirao Preto, BR-14040907 Ribeirao Preto, SP, BrazilUniv Guadalajara, Hlth Sci Univ Ctr, Inst Translat Nutrigenet & Nutrigen, Dept Mol Biol & Genom, Guadalajara 44100, Jalisco, MexicoUniv Estadual Campinas, UNICAMP, Sch Appl Sci, Lab Mol Biol Exercise LaBMEx, BR-13484350 Limeira, SP, BrazilState Univ Sao Paulo Julio de Mesquita Filho Arar, Postgrad Program Nutr Sci, BR-14800903 Araraquara, SP, BrazilFAPESP: 2017/09038-1MdpiUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Univ GuadalajaraUniversidade Estadual de Campinas (UNICAMP)Pinto, Ana P.Vieira, Tales S. [UNESP]Marafon, Bruno B.Batitucci, Gabriela [UNESP]Cabrera, Elisa M. B.Rocha, Alisson L. daKohama, Eike B.Rodrigues, Kellen C. C.Moura, Leandro P. dePauli, Jose R.Cintra, Dennys E.Ropelle, Eduardo R.Freitas, Ellen C. de [UNESP]Silva, Adelino S. R. da2020-12-10T17:32:08Z2020-12-10T17:32:08Z2020-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article16http://dx.doi.org/10.3390/nu12030641Nutrients. Basel: Mdpi, v. 12, n. 3, 16 p., 2020.http://hdl.handle.net/11449/19536710.3390/nu12030641WOS:000531831000050Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengNutrientsinfo:eu-repo/semantics/openAccess2021-10-23T08:10:55Zoai:repositorio.unesp.br:11449/195367Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T08:10:55Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The Combination of Fasting, Acute Resistance Exercise, and Protein Ingestion Led to Different Responses of Autophagy Markers in Gastrocnemius and Liver Samples |
title |
The Combination of Fasting, Acute Resistance Exercise, and Protein Ingestion Led to Different Responses of Autophagy Markers in Gastrocnemius and Liver Samples |
spellingShingle |
The Combination of Fasting, Acute Resistance Exercise, and Protein Ingestion Led to Different Responses of Autophagy Markers in Gastrocnemius and Liver Samples Pinto, Ana P. resistance exercise autophagy leucine mammalian target of rapamycin (mTOR) liver muscle |
title_short |
The Combination of Fasting, Acute Resistance Exercise, and Protein Ingestion Led to Different Responses of Autophagy Markers in Gastrocnemius and Liver Samples |
title_full |
The Combination of Fasting, Acute Resistance Exercise, and Protein Ingestion Led to Different Responses of Autophagy Markers in Gastrocnemius and Liver Samples |
title_fullStr |
The Combination of Fasting, Acute Resistance Exercise, and Protein Ingestion Led to Different Responses of Autophagy Markers in Gastrocnemius and Liver Samples |
title_full_unstemmed |
The Combination of Fasting, Acute Resistance Exercise, and Protein Ingestion Led to Different Responses of Autophagy Markers in Gastrocnemius and Liver Samples |
title_sort |
The Combination of Fasting, Acute Resistance Exercise, and Protein Ingestion Led to Different Responses of Autophagy Markers in Gastrocnemius and Liver Samples |
author |
Pinto, Ana P. |
author_facet |
Pinto, Ana P. Vieira, Tales S. [UNESP] Marafon, Bruno B. Batitucci, Gabriela [UNESP] Cabrera, Elisa M. B. Rocha, Alisson L. da Kohama, Eike B. Rodrigues, Kellen C. C. Moura, Leandro P. de Pauli, Jose R. Cintra, Dennys E. Ropelle, Eduardo R. Freitas, Ellen C. de [UNESP] Silva, Adelino S. R. da |
author_role |
author |
author2 |
Vieira, Tales S. [UNESP] Marafon, Bruno B. Batitucci, Gabriela [UNESP] Cabrera, Elisa M. B. Rocha, Alisson L. da Kohama, Eike B. Rodrigues, Kellen C. C. Moura, Leandro P. de Pauli, Jose R. Cintra, Dennys E. Ropelle, Eduardo R. Freitas, Ellen C. de [UNESP] Silva, Adelino S. R. da |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) Univ Guadalajara Universidade Estadual de Campinas (UNICAMP) |
dc.contributor.author.fl_str_mv |
Pinto, Ana P. Vieira, Tales S. [UNESP] Marafon, Bruno B. Batitucci, Gabriela [UNESP] Cabrera, Elisa M. B. Rocha, Alisson L. da Kohama, Eike B. Rodrigues, Kellen C. C. Moura, Leandro P. de Pauli, Jose R. Cintra, Dennys E. Ropelle, Eduardo R. Freitas, Ellen C. de [UNESP] Silva, Adelino S. R. da |
dc.subject.por.fl_str_mv |
resistance exercise autophagy leucine mammalian target of rapamycin (mTOR) liver muscle |
topic |
resistance exercise autophagy leucine mammalian target of rapamycin (mTOR) liver muscle |
description |
The present study verified the responses of proteins related to the autophagy pathway after 10 h of fast with resistance exercise and protein ingestion in skeletal muscle and liver samples. The rats were distributed into five experimental groups: control (CT; sedentary and without gavage after fast), exercise immediately (EXE-imm; after fast, rats were submitted to the resistance protocol and received water by gavage immediately after exercise), exercise after 1 h (EXE-1h; after fast, rats were submitted to the resistance protocol and received water by gavage 1 h after exercise), exercise and supplementation immediately after exercise (EXE/Suppl-imm; after fast, rats were submitted to the resistance protocol and received a mix of casein: whey protein 1:1 (w/w) by gavage immediately after exercise), exercise and supplementation 1 h after exercise (EXE/Suppl-1h; after fast, rats were submitted to the resistance protocol and received a mix of casein: whey protein 1:1 (w/w) by gavage 1 h after exercise). In summary, the current findings show that the combination of fasting, acute resistance exercise, and protein blend ingestion (immediately or 1 h after the exercise stimulus) increased the serum levels of leucine, insulin, and glucose, as well as the autophagy protein contents in skeletal muscle, but decreased other proteins related to the autophagic pathway in the liver. These results deserve further mechanistic investigations since athletes are combining fasting with physical exercise to enhance health and performance outcomes. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-10T17:32:08Z 2020-12-10T17:32:08Z 2020-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/nu12030641 Nutrients. Basel: Mdpi, v. 12, n. 3, 16 p., 2020. http://hdl.handle.net/11449/195367 10.3390/nu12030641 WOS:000531831000050 |
url |
http://dx.doi.org/10.3390/nu12030641 http://hdl.handle.net/11449/195367 |
identifier_str_mv |
Nutrients. Basel: Mdpi, v. 12, n. 3, 16 p., 2020. 10.3390/nu12030641 WOS:000531831000050 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Nutrients |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
16 |
dc.publisher.none.fl_str_mv |
Mdpi |
publisher.none.fl_str_mv |
Mdpi |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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_version_ |
1799965567810011136 |