Descrição do método de indução de diabetes mellitus para testes de novas drogas e procedimentos específicos

• Autor(a) principal: Araújo, Thalles Mayckon Vieira [UNESP]
• Data de Publicação: 2010
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/149430; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2017-01-26/000855239.pdf
• topic: Diabetes mellitus; Estreptozocina; Ratos

Diabetes mellitus is one of the commonest diseases found in the population, reaching 8 % of the world-wide population. With the growing longevity, obesity and sedentary of the population, the predominance of diabetes increases constantly. The term diabetes mellitus is characterized for hyperglycemia, resultant force of defects in the secretion or action of the insulin that has a critical paper in the regulation of the glucose in the blood. His deficiency provokes alterations in the metabolism of the carbohydrates and secundarily the metabolism of the proteins and lipids. These alterations can lead to the delay of the scarring of the wounds, to the increase of the predisposition to the infections and incidence of complications micro and macro-vascular. For the exposed one, preparation and balance is necessary between cost and benefit for surgical proceedings in the patient bearers of this pathology. So, completely I make an effort in order to investigate new technologies that minimize the harms brought by the disease it is of great importance. Through the induction of experimental diabetes we can test the action of new medicines and driving of therapeutic proceedings special for diabetics. The present work exposes the experimental methodology of induction of diabetes mellitus and application in mice. It consists of the use of male mice, Wistar, with 2 months and above 250 grammes fed with solid ration, except of the period of fast that precedes the administration of Streptozotocina. This drug causes the reduction in the synthesis of nicotinamida adenina dinucleotide - NAD, leading to the destruction of the cells metal vein of the pancreas and inhibiting the production of insulin. The only dose, when road was administered penile in the proportion of 35mg for each 1000g of physical animal weight, citrate is dissolved in tampon 0,01M and pH 4,5, after...