Benomyl sensitivity of isolates of Colletotrichum acutatum and C. gloeosporioides from citrus

Detalhes bibliográficos
Autor(a) principal: Peres, N. A R [UNESP]
Data de Publicação: 2004
Outros Autores: Souza, N. L. [UNESP], Peever, T. L., Timmer, L. W.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1094/PDIS.2004.88.2.125
http://hdl.handle.net/11449/67638
Resumo: Postbloom fruit drop (PFD) of citrus, caused by Colletotrichum acutatum, produces orange-brown lesions on petals and results in premature fruit drop and the retention of calyces. C. gloeosporioides is common in groves and causes postharvest anthracnose on fruit. Both diseases are controlled effectively by the fungicide benomyl in research fields and commercial orchards. Highly sensitive and resistant isolates of C. gloeosporioides were found, whereas all isolates of C. acutatum tested were moderately resistant. In preliminary studies conducted in vitro with three isolates of each, mycelial growth of sensitive isolates of C. gloeosporioides was inhibited completely by benomyl (Benlate 50 WP) at 1.0 μg/ml, whereas resistant isolates grew well at 10 μg/ml. Growth of all isolates of C. acutatum was inhibited by about 55% at 0.1 μg/ml and by 80% at 1.0 μg/ml. Spore germination of C. acutatum was inhibited more at 0.1 μg/ml than at 1.0 μg/ml or higher concentrations. In all, 20 isolates of C. acutatum from 17 groves and 20 isolates of C. gloeosporioides from 7 groves were collected from locations with different histories of benomyl usage in São Paulo, Brazil, and Florida, United States. Benomyl at 1.0 μ.g/ml completely inhibited growth of 133 isolates of C. gloeosporioides, with the exception of 7 isolates that were highly resistant to the fungicide, whereas all isolates of C. acutatum were only partially inhibited at 0.1 and 1.0 μg/ml. Analysis of variance indicated that the sensitivity of the isolates of C. acutatum was not affected by benomyl usage or grove of origin, and country of origin had only minor effects. No highly resistant or sensitive isolate of C. acutatum was recovered. Partial sequencing of the β-tubulin gene did not reveal nucleotide substitutions in codons 198 or 200 in C. acutatum that usually are associated with benomyl resistance in other fungi.
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spelling Benomyl sensitivity of isolates of Colletotrichum acutatum and C. gloeosporioides from citrusDisease controlFruitsGenesHarvestingSubstitution reactionsPetalsPlants (botany)Bacteria (microorganisms)CitrusColletotrichumFungiGlomerella acutataGlomerella cingulataPostbloom fruit drop (PFD) of citrus, caused by Colletotrichum acutatum, produces orange-brown lesions on petals and results in premature fruit drop and the retention of calyces. C. gloeosporioides is common in groves and causes postharvest anthracnose on fruit. Both diseases are controlled effectively by the fungicide benomyl in research fields and commercial orchards. Highly sensitive and resistant isolates of C. gloeosporioides were found, whereas all isolates of C. acutatum tested were moderately resistant. In preliminary studies conducted in vitro with three isolates of each, mycelial growth of sensitive isolates of C. gloeosporioides was inhibited completely by benomyl (Benlate 50 WP) at 1.0 μg/ml, whereas resistant isolates grew well at 10 μg/ml. Growth of all isolates of C. acutatum was inhibited by about 55% at 0.1 μg/ml and by 80% at 1.0 μg/ml. Spore germination of C. acutatum was inhibited more at 0.1 μg/ml than at 1.0 μg/ml or higher concentrations. In all, 20 isolates of C. acutatum from 17 groves and 20 isolates of C. gloeosporioides from 7 groves were collected from locations with different histories of benomyl usage in São Paulo, Brazil, and Florida, United States. Benomyl at 1.0 μ.g/ml completely inhibited growth of 133 isolates of C. gloeosporioides, with the exception of 7 isolates that were highly resistant to the fungicide, whereas all isolates of C. acutatum were only partially inhibited at 0.1 and 1.0 μg/ml. Analysis of variance indicated that the sensitivity of the isolates of C. acutatum was not affected by benomyl usage or grove of origin, and country of origin had only minor effects. No highly resistant or sensitive isolate of C. acutatum was recovered. Partial sequencing of the β-tubulin gene did not reveal nucleotide substitutions in codons 198 or 200 in C. acutatum that usually are associated with benomyl resistance in other fungi.Universidade Estadual Paulista FCA, Botucatu, SP, 18603-970Washington State University, Pullman, WA 99164-6430University of Florida Citrus Research and Education Center, Lake Alfred, FL 33850Instituto Biológico, Av. Conselheiro Rodrigues Alves 1252, São Paulo, SP, 04014-002Universidade Estadual Paulista FCA, Botucatu, SP, 18603-970Universidade Estadual Paulista (Unesp)Washington State UniversityCitrus Research and Education CenterInstituto BiológicoPeres, N. A R [UNESP]Souza, N. L. [UNESP]Peever, T. L.Timmer, L. W.2014-05-27T11:21:02Z2014-05-27T11:21:02Z2004-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article125-130http://dx.doi.org/10.1094/PDIS.2004.88.2.125Plant Disease, v. 88, n. 2, p. 125-130, 2004.0191-2917http://hdl.handle.net/11449/6763810.1094/PDIS.2004.88.2.125WOS:0002203135000042-s2.0-1642293895Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPlant Disease2.9410,575info:eu-repo/semantics/openAccess2021-10-23T11:43:35Zoai:repositorio.unesp.br:11449/67638Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:16:37.723597Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Benomyl sensitivity of isolates of Colletotrichum acutatum and C. gloeosporioides from citrus
title Benomyl sensitivity of isolates of Colletotrichum acutatum and C. gloeosporioides from citrus
spellingShingle Benomyl sensitivity of isolates of Colletotrichum acutatum and C. gloeosporioides from citrus
Peres, N. A R [UNESP]
Disease control
Fruits
Genes
Harvesting
Substitution reactions
Petals
Plants (botany)
Bacteria (microorganisms)
Citrus
Colletotrichum
Fungi
Glomerella acutata
Glomerella cingulata
title_short Benomyl sensitivity of isolates of Colletotrichum acutatum and C. gloeosporioides from citrus
title_full Benomyl sensitivity of isolates of Colletotrichum acutatum and C. gloeosporioides from citrus
title_fullStr Benomyl sensitivity of isolates of Colletotrichum acutatum and C. gloeosporioides from citrus
title_full_unstemmed Benomyl sensitivity of isolates of Colletotrichum acutatum and C. gloeosporioides from citrus
title_sort Benomyl sensitivity of isolates of Colletotrichum acutatum and C. gloeosporioides from citrus
author Peres, N. A R [UNESP]
author_facet Peres, N. A R [UNESP]
Souza, N. L. [UNESP]
Peever, T. L.
Timmer, L. W.
author_role author
author2 Souza, N. L. [UNESP]
Peever, T. L.
Timmer, L. W.
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Washington State University
Citrus Research and Education Center
Instituto Biológico
dc.contributor.author.fl_str_mv Peres, N. A R [UNESP]
Souza, N. L. [UNESP]
Peever, T. L.
Timmer, L. W.
dc.subject.por.fl_str_mv Disease control
Fruits
Genes
Harvesting
Substitution reactions
Petals
Plants (botany)
Bacteria (microorganisms)
Citrus
Colletotrichum
Fungi
Glomerella acutata
Glomerella cingulata
topic Disease control
Fruits
Genes
Harvesting
Substitution reactions
Petals
Plants (botany)
Bacteria (microorganisms)
Citrus
Colletotrichum
Fungi
Glomerella acutata
Glomerella cingulata
description Postbloom fruit drop (PFD) of citrus, caused by Colletotrichum acutatum, produces orange-brown lesions on petals and results in premature fruit drop and the retention of calyces. C. gloeosporioides is common in groves and causes postharvest anthracnose on fruit. Both diseases are controlled effectively by the fungicide benomyl in research fields and commercial orchards. Highly sensitive and resistant isolates of C. gloeosporioides were found, whereas all isolates of C. acutatum tested were moderately resistant. In preliminary studies conducted in vitro with three isolates of each, mycelial growth of sensitive isolates of C. gloeosporioides was inhibited completely by benomyl (Benlate 50 WP) at 1.0 μg/ml, whereas resistant isolates grew well at 10 μg/ml. Growth of all isolates of C. acutatum was inhibited by about 55% at 0.1 μg/ml and by 80% at 1.0 μg/ml. Spore germination of C. acutatum was inhibited more at 0.1 μg/ml than at 1.0 μg/ml or higher concentrations. In all, 20 isolates of C. acutatum from 17 groves and 20 isolates of C. gloeosporioides from 7 groves were collected from locations with different histories of benomyl usage in São Paulo, Brazil, and Florida, United States. Benomyl at 1.0 μ.g/ml completely inhibited growth of 133 isolates of C. gloeosporioides, with the exception of 7 isolates that were highly resistant to the fungicide, whereas all isolates of C. acutatum were only partially inhibited at 0.1 and 1.0 μg/ml. Analysis of variance indicated that the sensitivity of the isolates of C. acutatum was not affected by benomyl usage or grove of origin, and country of origin had only minor effects. No highly resistant or sensitive isolate of C. acutatum was recovered. Partial sequencing of the β-tubulin gene did not reveal nucleotide substitutions in codons 198 or 200 in C. acutatum that usually are associated with benomyl resistance in other fungi.
publishDate 2004
dc.date.none.fl_str_mv 2004-02-01
2014-05-27T11:21:02Z
2014-05-27T11:21:02Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1094/PDIS.2004.88.2.125
Plant Disease, v. 88, n. 2, p. 125-130, 2004.
0191-2917
http://hdl.handle.net/11449/67638
10.1094/PDIS.2004.88.2.125
WOS:000220313500004
2-s2.0-1642293895
url http://dx.doi.org/10.1094/PDIS.2004.88.2.125
http://hdl.handle.net/11449/67638
identifier_str_mv Plant Disease, v. 88, n. 2, p. 125-130, 2004.
0191-2917
10.1094/PDIS.2004.88.2.125
WOS:000220313500004
2-s2.0-1642293895
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Plant Disease
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dc.format.none.fl_str_mv 125-130
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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