The synthetic antimicrobial peptide IKR18 displays anti-infectious properties in Galleria mellonella in vivo model
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.bbagen.2022.130244 http://hdl.handle.net/11449/247676 |
Resumo: | Antimicrobial peptides (AMPs) are promising tools for developing new antibiotics. We described the design of IKR18, an AMP designed with the aid of computational tools. IKR18 showed antimicrobial activity against Gram-negative and Gram-positive bacteria, including methicillin-resistant Staphylococcus aureus (MRSA). CD studies revealed that IKR18 assumes an alpha-helical structure in the membrane-mimetic environment. The action mechanism IKR18 involves damage to the bacteria membrane, as demonstrated by Sytox green uptake. Furthermore, IKR18 displayed synergic and additive effects in combination with antibiotics ciprofloxacin and vancomycin. The peptide showed anti-biofilm activity in concentration and efficiency compared with commercial antibiotics, involving the direct death of bacteria, as confirmed by scanning electron microscopy. The anti-infective activity of IKR18 was demonstrated in the Galleria mellonella model infected with S. aureus, MRSA, and Acinetobacter baumannii. The novel bioinspired peptide, IKR18, proved to be effective in the control of bacterial infection, opening opportunities for the development of further assays, including preclinical models. |
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Repositório Institucional da UNESP |
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The synthetic antimicrobial peptide IKR18 displays anti-infectious properties in Galleria mellonella in vivo modelAnti-infective activityAntibiofilmBacteriaInfection modelMRSAAntimicrobial peptides (AMPs) are promising tools for developing new antibiotics. We described the design of IKR18, an AMP designed with the aid of computational tools. IKR18 showed antimicrobial activity against Gram-negative and Gram-positive bacteria, including methicillin-resistant Staphylococcus aureus (MRSA). CD studies revealed that IKR18 assumes an alpha-helical structure in the membrane-mimetic environment. The action mechanism IKR18 involves damage to the bacteria membrane, as demonstrated by Sytox green uptake. Furthermore, IKR18 displayed synergic and additive effects in combination with antibiotics ciprofloxacin and vancomycin. The peptide showed anti-biofilm activity in concentration and efficiency compared with commercial antibiotics, involving the direct death of bacteria, as confirmed by scanning electron microscopy. The anti-infective activity of IKR18 was demonstrated in the Galleria mellonella model infected with S. aureus, MRSA, and Acinetobacter baumannii. The novel bioinspired peptide, IKR18, proved to be effective in the control of bacterial infection, opening opportunities for the development of further assays, including preclinical models.Financiadora de Estudos e ProjetosConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Laboratório de Purificação de Proteínas e suas Funções Biológicas FACFAN Universidade Federal de Mato Grosso do SulInstituto de Química Departamento de Bioquímica e Química Tecnológica Universidade Estadual Paulista Júlio de Mesquita FilhoLaboratório de Nanomateriais e Nanotecnologia Aplicada (LNNA) Instituto de Física Universidade Federal de Mato Grosso do SulDepartamento de Biofísica Universidade Federal de São PauloInstituto de Química Departamento de Bioquímica e Química Tecnológica Universidade Estadual Paulista Júlio de Mesquita FilhoCNPq: 302175/2020–2CNPq: 305679/2016–3CNPq: 426912/2018–7CNPq: 430694/2016–4Universidade Federal de Mato Grosso do Sul (UFMS)Universidade Estadual Paulista (UNESP)Universidade Federal de São Paulo (UNIFESP)Ramalho, Suellen Rodriguesde Cássia Orlandi Sardi, JanainaJúnior, Edson Crusca [UNESP]Marchetto, Reinaldo [UNESP]Wender, HebertonVargas, Luiz Felipe Plaçade Miranda, AntonioAlmeida, Claudiane Vilharroelde Oliveira Almeida, Luís Henriquede Oliveira, Caio Fernando RamalhoMacedo, Maria Lígia Rodrigues2023-07-29T13:22:46Z2023-07-29T13:22:46Z2022-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.bbagen.2022.130244Biochimica et Biophysica Acta - General Subjects, v. 1866, n. 12, 2022.1872-80060304-4165http://hdl.handle.net/11449/24767610.1016/j.bbagen.2022.1302442-s2.0-85138820586Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiochimica et Biophysica Acta - General Subjectsinfo:eu-repo/semantics/openAccess2023-07-29T13:22:47Zoai:repositorio.unesp.br:11449/247676Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T13:22:47Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The synthetic antimicrobial peptide IKR18 displays anti-infectious properties in Galleria mellonella in vivo model |
title |
The synthetic antimicrobial peptide IKR18 displays anti-infectious properties in Galleria mellonella in vivo model |
spellingShingle |
The synthetic antimicrobial peptide IKR18 displays anti-infectious properties in Galleria mellonella in vivo model Ramalho, Suellen Rodrigues Anti-infective activity Antibiofilm Bacteria Infection model MRSA |
title_short |
The synthetic antimicrobial peptide IKR18 displays anti-infectious properties in Galleria mellonella in vivo model |
title_full |
The synthetic antimicrobial peptide IKR18 displays anti-infectious properties in Galleria mellonella in vivo model |
title_fullStr |
The synthetic antimicrobial peptide IKR18 displays anti-infectious properties in Galleria mellonella in vivo model |
title_full_unstemmed |
The synthetic antimicrobial peptide IKR18 displays anti-infectious properties in Galleria mellonella in vivo model |
title_sort |
The synthetic antimicrobial peptide IKR18 displays anti-infectious properties in Galleria mellonella in vivo model |
author |
Ramalho, Suellen Rodrigues |
author_facet |
Ramalho, Suellen Rodrigues de Cássia Orlandi Sardi, Janaina Júnior, Edson Crusca [UNESP] Marchetto, Reinaldo [UNESP] Wender, Heberton Vargas, Luiz Felipe Plaça de Miranda, Antonio Almeida, Claudiane Vilharroel de Oliveira Almeida, Luís Henrique de Oliveira, Caio Fernando Ramalho Macedo, Maria Lígia Rodrigues |
author_role |
author |
author2 |
de Cássia Orlandi Sardi, Janaina Júnior, Edson Crusca [UNESP] Marchetto, Reinaldo [UNESP] Wender, Heberton Vargas, Luiz Felipe Plaça de Miranda, Antonio Almeida, Claudiane Vilharroel de Oliveira Almeida, Luís Henrique de Oliveira, Caio Fernando Ramalho Macedo, Maria Lígia Rodrigues |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Mato Grosso do Sul (UFMS) Universidade Estadual Paulista (UNESP) Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Ramalho, Suellen Rodrigues de Cássia Orlandi Sardi, Janaina Júnior, Edson Crusca [UNESP] Marchetto, Reinaldo [UNESP] Wender, Heberton Vargas, Luiz Felipe Plaça de Miranda, Antonio Almeida, Claudiane Vilharroel de Oliveira Almeida, Luís Henrique de Oliveira, Caio Fernando Ramalho Macedo, Maria Lígia Rodrigues |
dc.subject.por.fl_str_mv |
Anti-infective activity Antibiofilm Bacteria Infection model MRSA |
topic |
Anti-infective activity Antibiofilm Bacteria Infection model MRSA |
description |
Antimicrobial peptides (AMPs) are promising tools for developing new antibiotics. We described the design of IKR18, an AMP designed with the aid of computational tools. IKR18 showed antimicrobial activity against Gram-negative and Gram-positive bacteria, including methicillin-resistant Staphylococcus aureus (MRSA). CD studies revealed that IKR18 assumes an alpha-helical structure in the membrane-mimetic environment. The action mechanism IKR18 involves damage to the bacteria membrane, as demonstrated by Sytox green uptake. Furthermore, IKR18 displayed synergic and additive effects in combination with antibiotics ciprofloxacin and vancomycin. The peptide showed anti-biofilm activity in concentration and efficiency compared with commercial antibiotics, involving the direct death of bacteria, as confirmed by scanning electron microscopy. The anti-infective activity of IKR18 was demonstrated in the Galleria mellonella model infected with S. aureus, MRSA, and Acinetobacter baumannii. The novel bioinspired peptide, IKR18, proved to be effective in the control of bacterial infection, opening opportunities for the development of further assays, including preclinical models. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-12-01 2023-07-29T13:22:46Z 2023-07-29T13:22:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.bbagen.2022.130244 Biochimica et Biophysica Acta - General Subjects, v. 1866, n. 12, 2022. 1872-8006 0304-4165 http://hdl.handle.net/11449/247676 10.1016/j.bbagen.2022.130244 2-s2.0-85138820586 |
url |
http://dx.doi.org/10.1016/j.bbagen.2022.130244 http://hdl.handle.net/11449/247676 |
identifier_str_mv |
Biochimica et Biophysica Acta - General Subjects, v. 1866, n. 12, 2022. 1872-8006 0304-4165 10.1016/j.bbagen.2022.130244 2-s2.0-85138820586 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biochimica et Biophysica Acta - General Subjects |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799964400630628352 |