Recurrence of COVID-19 associated with reduced T-cell responses in a monozygotic twin pair

Detalhes bibliográficos
Autor(a) principal: De Castro, Mateus V.
Data de Publicação: 2022
Outros Autores: Santos, Keity S., Apostolico, Juliana S., Fernandes, Edgar R., Almeida, Rafael R., Levin, Gabriel, Magawa, Jhosiene Y., Nunes, João Paulo S., Bruni, Mirian, Yamamoto, Marcio M., Lima, Ariane C., Silva, Monize V. R., Matos, Larissa R. B., Coria, Vivian R., Castelli, Erick C. [UNESP], Scliar, Marilia O., Kuramoto, Andreia, Bruno, Fernanda R., Jacintho, Lucas C., Nunes, Kelly, Wang, Jaqueline Y. T., Coelho, Veronica P., Neto, Miguel Mitne, Maciel, Rui M. B., Naslavsky, Michel S., Passos-Bueno, Maria Rita, Boscardin, Silvia B., Rosa, Daniela S., Kalil, Jorge, Zatz, Mayana, Cunha-Neto, Edecio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1098/rsob.210240
http://hdl.handle.net/11449/231612
Resumo: Recurrence of COVID-19 in recovered patients has been increasingly reported. However, the immune mechanisms behind the recurrence have not been thoroughly investigated. The presence of neutralizing antibodies (nAbs) in recurrence/reinfection cases suggests that other types of immune response are involved in protection against recurrence. Here, we investigated the innate type I/III interferon (IFN) response, binding and nAb assays and T-cell responses to severe acute respiratory distress syndrome coronavirus 2 (SARS-CoV-2) with IFN gamma (IFNγ) enzyme-linked spot assay (ELISPOT) in three pairs of young adult monozygotic (MZ) twins with previous confirmed COVID-19, one of them presenting a severe recurrence four months after the initial infection. Twin studies have been of paramount importance to comprehend the immunogenetics of infectious diseases. Each MZ twin pair was previously exposed to SARS-CoV-2, as seen by clinical reports. The six individuals presented similar overall recovered immune responses except for the recurrence case, who presented a drastically reduced number of recognized SARS-CoV-2 T-cell epitopes on ELISPOT as compared to her twin sister and the other twin pairs. Our results suggest that the lack of a broad T-cell response to initial infection may have led to recurrence, emphasizing that an effective SARS-CoV-2-specific T-cell immune response is key for complete viral control and avoidance of clinical recurrence of COVID-19.
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spelling Recurrence of COVID-19 associated with reduced T-cell responses in a monozygotic twin pairCOVID-19immunityrecurrencesevere acute respiratory distress syndrome coronavirus 2T celltwinsRecurrence of COVID-19 in recovered patients has been increasingly reported. However, the immune mechanisms behind the recurrence have not been thoroughly investigated. The presence of neutralizing antibodies (nAbs) in recurrence/reinfection cases suggests that other types of immune response are involved in protection against recurrence. Here, we investigated the innate type I/III interferon (IFN) response, binding and nAb assays and T-cell responses to severe acute respiratory distress syndrome coronavirus 2 (SARS-CoV-2) with IFN gamma (IFNγ) enzyme-linked spot assay (ELISPOT) in three pairs of young adult monozygotic (MZ) twins with previous confirmed COVID-19, one of them presenting a severe recurrence four months after the initial infection. Twin studies have been of paramount importance to comprehend the immunogenetics of infectious diseases. Each MZ twin pair was previously exposed to SARS-CoV-2, as seen by clinical reports. The six individuals presented similar overall recovered immune responses except for the recurrence case, who presented a drastically reduced number of recognized SARS-CoV-2 T-cell epitopes on ELISPOT as compared to her twin sister and the other twin pairs. Our results suggest that the lack of a broad T-cell response to initial infection may have led to recurrence, emphasizing that an effective SARS-CoV-2-specific T-cell immune response is key for complete viral control and avoidance of clinical recurrence of COVID-19.Human Genome and Stem Cell Research Center (HUG-CELL) Biosciences Institute Universidade de São Paulo, SPLaboratory of Immunology Heart Institute (InCor) Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo (HCFMUSP), SPInstitute for Investigation in Immunology Instituto Nacional de Ciência e Tecnologia - Iii-INCT, SPDivision of Clinical Immunology and Allergy Department of Medicine Faculdade de Medicina da Universidade de São Paulo (FMUSP), SPDepartment of Microbiology Immunology and Parasitology Universidade Federal de São Paulo (UNIFESP/EPM), SPDepartment of Parasitology Biosciences Institute Universidade de São Paulo, SPSchool of Medicine Universidade Estadual Paulista (UNESP), SPFleury Laboratory, SPSchool of Medicine Universidade Estadual Paulista (UNESP), SPUniversidade de São Paulo (USP)Instituto Nacional de Ciência e Tecnologia - Iii-INCTUniversidade Federal de São Paulo (UNIFESP)Universidade Estadual Paulista (UNESP)Fleury LaboratoryDe Castro, Mateus V.Santos, Keity S.Apostolico, Juliana S.Fernandes, Edgar R.Almeida, Rafael R.Levin, GabrielMagawa, Jhosiene Y.Nunes, João Paulo S.Bruni, MirianYamamoto, Marcio M.Lima, Ariane C.Silva, Monize V. R.Matos, Larissa R. B.Coria, Vivian R.Castelli, Erick C. [UNESP]Scliar, Marilia O.Kuramoto, AndreiaBruno, Fernanda R.Jacintho, Lucas C.Nunes, KellyWang, Jaqueline Y. T.Coelho, Veronica P.Neto, Miguel MitneMaciel, Rui M. B.Naslavsky, Michel S.Passos-Bueno, Maria RitaBoscardin, Silvia B.Rosa, Daniela S.Kalil, JorgeZatz, MayanaCunha-Neto, Edecio2022-04-29T08:46:34Z2022-04-29T08:46:34Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1098/rsob.210240Open Biology, v. 12, n. 2, 2022.2046-2441http://hdl.handle.net/11449/23161210.1098/rsob.2102402-s2.0-85124058318Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengOpen Biologyinfo:eu-repo/semantics/openAccess2022-04-29T08:46:35Zoai:repositorio.unesp.br:11449/231612Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:14:12.865587Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Recurrence of COVID-19 associated with reduced T-cell responses in a monozygotic twin pair
title Recurrence of COVID-19 associated with reduced T-cell responses in a monozygotic twin pair
spellingShingle Recurrence of COVID-19 associated with reduced T-cell responses in a monozygotic twin pair
De Castro, Mateus V.
COVID-19
immunity
recurrence
severe acute respiratory distress syndrome coronavirus 2
T cell
twins
title_short Recurrence of COVID-19 associated with reduced T-cell responses in a monozygotic twin pair
title_full Recurrence of COVID-19 associated with reduced T-cell responses in a monozygotic twin pair
title_fullStr Recurrence of COVID-19 associated with reduced T-cell responses in a monozygotic twin pair
title_full_unstemmed Recurrence of COVID-19 associated with reduced T-cell responses in a monozygotic twin pair
title_sort Recurrence of COVID-19 associated with reduced T-cell responses in a monozygotic twin pair
author De Castro, Mateus V.
author_facet De Castro, Mateus V.
Santos, Keity S.
Apostolico, Juliana S.
Fernandes, Edgar R.
Almeida, Rafael R.
Levin, Gabriel
Magawa, Jhosiene Y.
Nunes, João Paulo S.
Bruni, Mirian
Yamamoto, Marcio M.
Lima, Ariane C.
Silva, Monize V. R.
Matos, Larissa R. B.
Coria, Vivian R.
Castelli, Erick C. [UNESP]
Scliar, Marilia O.
Kuramoto, Andreia
Bruno, Fernanda R.
Jacintho, Lucas C.
Nunes, Kelly
Wang, Jaqueline Y. T.
Coelho, Veronica P.
Neto, Miguel Mitne
Maciel, Rui M. B.
Naslavsky, Michel S.
Passos-Bueno, Maria Rita
Boscardin, Silvia B.
Rosa, Daniela S.
Kalil, Jorge
Zatz, Mayana
Cunha-Neto, Edecio
author_role author
author2 Santos, Keity S.
Apostolico, Juliana S.
Fernandes, Edgar R.
Almeida, Rafael R.
Levin, Gabriel
Magawa, Jhosiene Y.
Nunes, João Paulo S.
Bruni, Mirian
Yamamoto, Marcio M.
Lima, Ariane C.
Silva, Monize V. R.
Matos, Larissa R. B.
Coria, Vivian R.
Castelli, Erick C. [UNESP]
Scliar, Marilia O.
Kuramoto, Andreia
Bruno, Fernanda R.
Jacintho, Lucas C.
Nunes, Kelly
Wang, Jaqueline Y. T.
Coelho, Veronica P.
Neto, Miguel Mitne
Maciel, Rui M. B.
Naslavsky, Michel S.
Passos-Bueno, Maria Rita
Boscardin, Silvia B.
Rosa, Daniela S.
Kalil, Jorge
Zatz, Mayana
Cunha-Neto, Edecio
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Instituto Nacional de Ciência e Tecnologia - Iii-INCT
Universidade Federal de São Paulo (UNIFESP)
Universidade Estadual Paulista (UNESP)
Fleury Laboratory
dc.contributor.author.fl_str_mv De Castro, Mateus V.
Santos, Keity S.
Apostolico, Juliana S.
Fernandes, Edgar R.
Almeida, Rafael R.
Levin, Gabriel
Magawa, Jhosiene Y.
Nunes, João Paulo S.
Bruni, Mirian
Yamamoto, Marcio M.
Lima, Ariane C.
Silva, Monize V. R.
Matos, Larissa R. B.
Coria, Vivian R.
Castelli, Erick C. [UNESP]
Scliar, Marilia O.
Kuramoto, Andreia
Bruno, Fernanda R.
Jacintho, Lucas C.
Nunes, Kelly
Wang, Jaqueline Y. T.
Coelho, Veronica P.
Neto, Miguel Mitne
Maciel, Rui M. B.
Naslavsky, Michel S.
Passos-Bueno, Maria Rita
Boscardin, Silvia B.
Rosa, Daniela S.
Kalil, Jorge
Zatz, Mayana
Cunha-Neto, Edecio
dc.subject.por.fl_str_mv COVID-19
immunity
recurrence
severe acute respiratory distress syndrome coronavirus 2
T cell
twins
topic COVID-19
immunity
recurrence
severe acute respiratory distress syndrome coronavirus 2
T cell
twins
description Recurrence of COVID-19 in recovered patients has been increasingly reported. However, the immune mechanisms behind the recurrence have not been thoroughly investigated. The presence of neutralizing antibodies (nAbs) in recurrence/reinfection cases suggests that other types of immune response are involved in protection against recurrence. Here, we investigated the innate type I/III interferon (IFN) response, binding and nAb assays and T-cell responses to severe acute respiratory distress syndrome coronavirus 2 (SARS-CoV-2) with IFN gamma (IFNγ) enzyme-linked spot assay (ELISPOT) in three pairs of young adult monozygotic (MZ) twins with previous confirmed COVID-19, one of them presenting a severe recurrence four months after the initial infection. Twin studies have been of paramount importance to comprehend the immunogenetics of infectious diseases. Each MZ twin pair was previously exposed to SARS-CoV-2, as seen by clinical reports. The six individuals presented similar overall recovered immune responses except for the recurrence case, who presented a drastically reduced number of recognized SARS-CoV-2 T-cell epitopes on ELISPOT as compared to her twin sister and the other twin pairs. Our results suggest that the lack of a broad T-cell response to initial infection may have led to recurrence, emphasizing that an effective SARS-CoV-2-specific T-cell immune response is key for complete viral control and avoidance of clinical recurrence of COVID-19.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-29T08:46:34Z
2022-04-29T08:46:34Z
2022-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1098/rsob.210240
Open Biology, v. 12, n. 2, 2022.
2046-2441
http://hdl.handle.net/11449/231612
10.1098/rsob.210240
2-s2.0-85124058318
url http://dx.doi.org/10.1098/rsob.210240
http://hdl.handle.net/11449/231612
identifier_str_mv Open Biology, v. 12, n. 2, 2022.
2046-2441
10.1098/rsob.210240
2-s2.0-85124058318
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Open Biology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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