Recurrence of COVID-19 associated with reduced T-cell responses in a monozygotic twin pair
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1098/rsob.210240 http://hdl.handle.net/11449/231612 |
Resumo: | Recurrence of COVID-19 in recovered patients has been increasingly reported. However, the immune mechanisms behind the recurrence have not been thoroughly investigated. The presence of neutralizing antibodies (nAbs) in recurrence/reinfection cases suggests that other types of immune response are involved in protection against recurrence. Here, we investigated the innate type I/III interferon (IFN) response, binding and nAb assays and T-cell responses to severe acute respiratory distress syndrome coronavirus 2 (SARS-CoV-2) with IFN gamma (IFNγ) enzyme-linked spot assay (ELISPOT) in three pairs of young adult monozygotic (MZ) twins with previous confirmed COVID-19, one of them presenting a severe recurrence four months after the initial infection. Twin studies have been of paramount importance to comprehend the immunogenetics of infectious diseases. Each MZ twin pair was previously exposed to SARS-CoV-2, as seen by clinical reports. The six individuals presented similar overall recovered immune responses except for the recurrence case, who presented a drastically reduced number of recognized SARS-CoV-2 T-cell epitopes on ELISPOT as compared to her twin sister and the other twin pairs. Our results suggest that the lack of a broad T-cell response to initial infection may have led to recurrence, emphasizing that an effective SARS-CoV-2-specific T-cell immune response is key for complete viral control and avoidance of clinical recurrence of COVID-19. |
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Recurrence of COVID-19 associated with reduced T-cell responses in a monozygotic twin pairCOVID-19immunityrecurrencesevere acute respiratory distress syndrome coronavirus 2T celltwinsRecurrence of COVID-19 in recovered patients has been increasingly reported. However, the immune mechanisms behind the recurrence have not been thoroughly investigated. The presence of neutralizing antibodies (nAbs) in recurrence/reinfection cases suggests that other types of immune response are involved in protection against recurrence. Here, we investigated the innate type I/III interferon (IFN) response, binding and nAb assays and T-cell responses to severe acute respiratory distress syndrome coronavirus 2 (SARS-CoV-2) with IFN gamma (IFNγ) enzyme-linked spot assay (ELISPOT) in three pairs of young adult monozygotic (MZ) twins with previous confirmed COVID-19, one of them presenting a severe recurrence four months after the initial infection. Twin studies have been of paramount importance to comprehend the immunogenetics of infectious diseases. Each MZ twin pair was previously exposed to SARS-CoV-2, as seen by clinical reports. The six individuals presented similar overall recovered immune responses except for the recurrence case, who presented a drastically reduced number of recognized SARS-CoV-2 T-cell epitopes on ELISPOT as compared to her twin sister and the other twin pairs. Our results suggest that the lack of a broad T-cell response to initial infection may have led to recurrence, emphasizing that an effective SARS-CoV-2-specific T-cell immune response is key for complete viral control and avoidance of clinical recurrence of COVID-19.Human Genome and Stem Cell Research Center (HUG-CELL) Biosciences Institute Universidade de São Paulo, SPLaboratory of Immunology Heart Institute (InCor) Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo (HCFMUSP), SPInstitute for Investigation in Immunology Instituto Nacional de Ciência e Tecnologia - Iii-INCT, SPDivision of Clinical Immunology and Allergy Department of Medicine Faculdade de Medicina da Universidade de São Paulo (FMUSP), SPDepartment of Microbiology Immunology and Parasitology Universidade Federal de São Paulo (UNIFESP/EPM), SPDepartment of Parasitology Biosciences Institute Universidade de São Paulo, SPSchool of Medicine Universidade Estadual Paulista (UNESP), SPFleury Laboratory, SPSchool of Medicine Universidade Estadual Paulista (UNESP), SPUniversidade de São Paulo (USP)Instituto Nacional de Ciência e Tecnologia - Iii-INCTUniversidade Federal de São Paulo (UNIFESP)Universidade Estadual Paulista (UNESP)Fleury LaboratoryDe Castro, Mateus V.Santos, Keity S.Apostolico, Juliana S.Fernandes, Edgar R.Almeida, Rafael R.Levin, GabrielMagawa, Jhosiene Y.Nunes, João Paulo S.Bruni, MirianYamamoto, Marcio M.Lima, Ariane C.Silva, Monize V. R.Matos, Larissa R. B.Coria, Vivian R.Castelli, Erick C. [UNESP]Scliar, Marilia O.Kuramoto, AndreiaBruno, Fernanda R.Jacintho, Lucas C.Nunes, KellyWang, Jaqueline Y. T.Coelho, Veronica P.Neto, Miguel MitneMaciel, Rui M. B.Naslavsky, Michel S.Passos-Bueno, Maria RitaBoscardin, Silvia B.Rosa, Daniela S.Kalil, JorgeZatz, MayanaCunha-Neto, Edecio2022-04-29T08:46:34Z2022-04-29T08:46:34Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1098/rsob.210240Open Biology, v. 12, n. 2, 2022.2046-2441http://hdl.handle.net/11449/23161210.1098/rsob.2102402-s2.0-85124058318Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengOpen Biologyinfo:eu-repo/semantics/openAccess2022-04-29T08:46:35Zoai:repositorio.unesp.br:11449/231612Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:14:12.865587Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Recurrence of COVID-19 associated with reduced T-cell responses in a monozygotic twin pair |
title |
Recurrence of COVID-19 associated with reduced T-cell responses in a monozygotic twin pair |
spellingShingle |
Recurrence of COVID-19 associated with reduced T-cell responses in a monozygotic twin pair De Castro, Mateus V. COVID-19 immunity recurrence severe acute respiratory distress syndrome coronavirus 2 T cell twins |
title_short |
Recurrence of COVID-19 associated with reduced T-cell responses in a monozygotic twin pair |
title_full |
Recurrence of COVID-19 associated with reduced T-cell responses in a monozygotic twin pair |
title_fullStr |
Recurrence of COVID-19 associated with reduced T-cell responses in a monozygotic twin pair |
title_full_unstemmed |
Recurrence of COVID-19 associated with reduced T-cell responses in a monozygotic twin pair |
title_sort |
Recurrence of COVID-19 associated with reduced T-cell responses in a monozygotic twin pair |
author |
De Castro, Mateus V. |
author_facet |
De Castro, Mateus V. Santos, Keity S. Apostolico, Juliana S. Fernandes, Edgar R. Almeida, Rafael R. Levin, Gabriel Magawa, Jhosiene Y. Nunes, João Paulo S. Bruni, Mirian Yamamoto, Marcio M. Lima, Ariane C. Silva, Monize V. R. Matos, Larissa R. B. Coria, Vivian R. Castelli, Erick C. [UNESP] Scliar, Marilia O. Kuramoto, Andreia Bruno, Fernanda R. Jacintho, Lucas C. Nunes, Kelly Wang, Jaqueline Y. T. Coelho, Veronica P. Neto, Miguel Mitne Maciel, Rui M. B. Naslavsky, Michel S. Passos-Bueno, Maria Rita Boscardin, Silvia B. Rosa, Daniela S. Kalil, Jorge Zatz, Mayana Cunha-Neto, Edecio |
author_role |
author |
author2 |
Santos, Keity S. Apostolico, Juliana S. Fernandes, Edgar R. Almeida, Rafael R. Levin, Gabriel Magawa, Jhosiene Y. Nunes, João Paulo S. Bruni, Mirian Yamamoto, Marcio M. Lima, Ariane C. Silva, Monize V. R. Matos, Larissa R. B. Coria, Vivian R. Castelli, Erick C. [UNESP] Scliar, Marilia O. Kuramoto, Andreia Bruno, Fernanda R. Jacintho, Lucas C. Nunes, Kelly Wang, Jaqueline Y. T. Coelho, Veronica P. Neto, Miguel Mitne Maciel, Rui M. B. Naslavsky, Michel S. Passos-Bueno, Maria Rita Boscardin, Silvia B. Rosa, Daniela S. Kalil, Jorge Zatz, Mayana Cunha-Neto, Edecio |
author2_role |
author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Instituto Nacional de Ciência e Tecnologia - Iii-INCT Universidade Federal de São Paulo (UNIFESP) Universidade Estadual Paulista (UNESP) Fleury Laboratory |
dc.contributor.author.fl_str_mv |
De Castro, Mateus V. Santos, Keity S. Apostolico, Juliana S. Fernandes, Edgar R. Almeida, Rafael R. Levin, Gabriel Magawa, Jhosiene Y. Nunes, João Paulo S. Bruni, Mirian Yamamoto, Marcio M. Lima, Ariane C. Silva, Monize V. R. Matos, Larissa R. B. Coria, Vivian R. Castelli, Erick C. [UNESP] Scliar, Marilia O. Kuramoto, Andreia Bruno, Fernanda R. Jacintho, Lucas C. Nunes, Kelly Wang, Jaqueline Y. T. Coelho, Veronica P. Neto, Miguel Mitne Maciel, Rui M. B. Naslavsky, Michel S. Passos-Bueno, Maria Rita Boscardin, Silvia B. Rosa, Daniela S. Kalil, Jorge Zatz, Mayana Cunha-Neto, Edecio |
dc.subject.por.fl_str_mv |
COVID-19 immunity recurrence severe acute respiratory distress syndrome coronavirus 2 T cell twins |
topic |
COVID-19 immunity recurrence severe acute respiratory distress syndrome coronavirus 2 T cell twins |
description |
Recurrence of COVID-19 in recovered patients has been increasingly reported. However, the immune mechanisms behind the recurrence have not been thoroughly investigated. The presence of neutralizing antibodies (nAbs) in recurrence/reinfection cases suggests that other types of immune response are involved in protection against recurrence. Here, we investigated the innate type I/III interferon (IFN) response, binding and nAb assays and T-cell responses to severe acute respiratory distress syndrome coronavirus 2 (SARS-CoV-2) with IFN gamma (IFNγ) enzyme-linked spot assay (ELISPOT) in three pairs of young adult monozygotic (MZ) twins with previous confirmed COVID-19, one of them presenting a severe recurrence four months after the initial infection. Twin studies have been of paramount importance to comprehend the immunogenetics of infectious diseases. Each MZ twin pair was previously exposed to SARS-CoV-2, as seen by clinical reports. The six individuals presented similar overall recovered immune responses except for the recurrence case, who presented a drastically reduced number of recognized SARS-CoV-2 T-cell epitopes on ELISPOT as compared to her twin sister and the other twin pairs. Our results suggest that the lack of a broad T-cell response to initial infection may have led to recurrence, emphasizing that an effective SARS-CoV-2-specific T-cell immune response is key for complete viral control and avoidance of clinical recurrence of COVID-19. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-29T08:46:34Z 2022-04-29T08:46:34Z 2022-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1098/rsob.210240 Open Biology, v. 12, n. 2, 2022. 2046-2441 http://hdl.handle.net/11449/231612 10.1098/rsob.210240 2-s2.0-85124058318 |
url |
http://dx.doi.org/10.1098/rsob.210240 http://hdl.handle.net/11449/231612 |
identifier_str_mv |
Open Biology, v. 12, n. 2, 2022. 2046-2441 10.1098/rsob.210240 2-s2.0-85124058318 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Open Biology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128485467619328 |