Environmental persistence and virulence of Salmonella spp. Isolated from a poultry slaughterhouse
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.foodres.2019.108835 http://hdl.handle.net/11449/196636 |
Resumo: | Salmonella spp. is responsible for severe foodborne disease, and is one of the main agents involved in foodborne outbreaks worldwide. Contamination occurs mainly as a result of poultry and egg consumption since they can carry some serotypes pathogenic to humans. The aim of the study was to evaluate the persistence and pathogenic potential of Salmonella spp. (n = 40) isolated from poultry slaughterhouse mats, using adhesion and invasion assays, antimicrobial susceptibility by disc diffusion, and biofilm production as phenotypic tests and genotypic analyses. Polystyrene mats presented 3.2 times greater chance of isolating Salmonella than canvas mats. Besides, we observed resistance to tetracycline (17.5%), ampicillin (10%), cefotaxime (7.5%), trimethoprim-sulfamethoxazole (5%), and chloramphenicol (2.5%). All strains possessed the invA, sipB, sipD, ssaR, sifA, sitC, iroN, tolC, flgK, fljB, and flgL genes. The genes sopB and sipA were both present in 92.5% of the isolates, while sopD and spvB were observed in 90% and 32.5% of strains, respectively. All strains adhered to and invaded HeLa cells. Regarding biofilm production, 31 (77.5%) strains were able to produce biofilm on polystyrene microplates. Using PFGE, we detected the persistence of clones in the environment for up to 18 from the 20 weeks. The ability of these strains to produce a biofilm and thus persist in the environment and disperse through contact surfaces in the processing plant favors the contamination of food, aggravated by the pathogenic potential of these isolates demonstrated by their adhesion capacity, invasion and resistance to various antibiotic agents. |
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Environmental persistence and virulence of Salmonella spp. Isolated from a poultry slaughterhouseAntibiotic resistanceBiofilmInvasionPFGEVirulence genesSalmonella spp. is responsible for severe foodborne disease, and is one of the main agents involved in foodborne outbreaks worldwide. Contamination occurs mainly as a result of poultry and egg consumption since they can carry some serotypes pathogenic to humans. The aim of the study was to evaluate the persistence and pathogenic potential of Salmonella spp. (n = 40) isolated from poultry slaughterhouse mats, using adhesion and invasion assays, antimicrobial susceptibility by disc diffusion, and biofilm production as phenotypic tests and genotypic analyses. Polystyrene mats presented 3.2 times greater chance of isolating Salmonella than canvas mats. Besides, we observed resistance to tetracycline (17.5%), ampicillin (10%), cefotaxime (7.5%), trimethoprim-sulfamethoxazole (5%), and chloramphenicol (2.5%). All strains possessed the invA, sipB, sipD, ssaR, sifA, sitC, iroN, tolC, flgK, fljB, and flgL genes. The genes sopB and sipA were both present in 92.5% of the isolates, while sopD and spvB were observed in 90% and 32.5% of strains, respectively. All strains adhered to and invaded HeLa cells. Regarding biofilm production, 31 (77.5%) strains were able to produce biofilm on polystyrene microplates. Using PFGE, we detected the persistence of clones in the environment for up to 18 from the 20 weeks. The ability of these strains to produce a biofilm and thus persist in the environment and disperse through contact surfaces in the processing plant favors the contamination of food, aggravated by the pathogenic potential of these isolates demonstrated by their adhesion capacity, invasion and resistance to various antibiotic agents.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Sao Paulo State Univ, Inst Biosci, Dept Microbiol & Immunol, Botucatu, SP, BrazilAdolfo Lutz Inst, Bacteriol Div, Sao Paulo, SP, BrazilSao Paulo State Univ, Fac Vet Med, Dept Vet Hyg & Publ Hlth, Botucatu, SP, BrazilSao Paulo State Univ, Inst Biosci, Dept Microbiol & Immunol, Botucatu, SP, BrazilSao Paulo State Univ, Fac Vet Med, Dept Vet Hyg & Publ Hlth, Botucatu, SP, BrazilCAPES: 001Elsevier B.V.Universidade Estadual Paulista (Unesp)Adolfo Lutz InstDantas, Stefani T. A. [UNESP]Camargo, Carlos H.Tiba-Casas, Monique R.Vivian, Ricardo C. [UNESP]Pinto, Jose P. A. N. [UNESP]Pantoja, Jose C. F. [UNESP]Hernandes, Rodrigo T. [UNESP]Fernandes Junior, Ary [UNESP]Rall, Vera L. M. [UNESP]2020-12-10T19:51:18Z2020-12-10T19:51:18Z2020-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article8http://dx.doi.org/10.1016/j.foodres.2019.108835Food Research International. Amsterdam: Elsevier, v. 129, 8 p., 2020.0963-9969http://hdl.handle.net/11449/19663610.1016/j.foodres.2019.108835WOS:000517665500024Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFood Research Internationalinfo:eu-repo/semantics/openAccess2021-10-23T08:53:39Zoai:repositorio.unesp.br:11449/196636Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T08:53:39Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Environmental persistence and virulence of Salmonella spp. Isolated from a poultry slaughterhouse |
title |
Environmental persistence and virulence of Salmonella spp. Isolated from a poultry slaughterhouse |
spellingShingle |
Environmental persistence and virulence of Salmonella spp. Isolated from a poultry slaughterhouse Dantas, Stefani T. A. [UNESP] Antibiotic resistance Biofilm Invasion PFGE Virulence genes |
title_short |
Environmental persistence and virulence of Salmonella spp. Isolated from a poultry slaughterhouse |
title_full |
Environmental persistence and virulence of Salmonella spp. Isolated from a poultry slaughterhouse |
title_fullStr |
Environmental persistence and virulence of Salmonella spp. Isolated from a poultry slaughterhouse |
title_full_unstemmed |
Environmental persistence and virulence of Salmonella spp. Isolated from a poultry slaughterhouse |
title_sort |
Environmental persistence and virulence of Salmonella spp. Isolated from a poultry slaughterhouse |
author |
Dantas, Stefani T. A. [UNESP] |
author_facet |
Dantas, Stefani T. A. [UNESP] Camargo, Carlos H. Tiba-Casas, Monique R. Vivian, Ricardo C. [UNESP] Pinto, Jose P. A. N. [UNESP] Pantoja, Jose C. F. [UNESP] Hernandes, Rodrigo T. [UNESP] Fernandes Junior, Ary [UNESP] Rall, Vera L. M. [UNESP] |
author_role |
author |
author2 |
Camargo, Carlos H. Tiba-Casas, Monique R. Vivian, Ricardo C. [UNESP] Pinto, Jose P. A. N. [UNESP] Pantoja, Jose C. F. [UNESP] Hernandes, Rodrigo T. [UNESP] Fernandes Junior, Ary [UNESP] Rall, Vera L. M. [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Adolfo Lutz Inst |
dc.contributor.author.fl_str_mv |
Dantas, Stefani T. A. [UNESP] Camargo, Carlos H. Tiba-Casas, Monique R. Vivian, Ricardo C. [UNESP] Pinto, Jose P. A. N. [UNESP] Pantoja, Jose C. F. [UNESP] Hernandes, Rodrigo T. [UNESP] Fernandes Junior, Ary [UNESP] Rall, Vera L. M. [UNESP] |
dc.subject.por.fl_str_mv |
Antibiotic resistance Biofilm Invasion PFGE Virulence genes |
topic |
Antibiotic resistance Biofilm Invasion PFGE Virulence genes |
description |
Salmonella spp. is responsible for severe foodborne disease, and is one of the main agents involved in foodborne outbreaks worldwide. Contamination occurs mainly as a result of poultry and egg consumption since they can carry some serotypes pathogenic to humans. The aim of the study was to evaluate the persistence and pathogenic potential of Salmonella spp. (n = 40) isolated from poultry slaughterhouse mats, using adhesion and invasion assays, antimicrobial susceptibility by disc diffusion, and biofilm production as phenotypic tests and genotypic analyses. Polystyrene mats presented 3.2 times greater chance of isolating Salmonella than canvas mats. Besides, we observed resistance to tetracycline (17.5%), ampicillin (10%), cefotaxime (7.5%), trimethoprim-sulfamethoxazole (5%), and chloramphenicol (2.5%). All strains possessed the invA, sipB, sipD, ssaR, sifA, sitC, iroN, tolC, flgK, fljB, and flgL genes. The genes sopB and sipA were both present in 92.5% of the isolates, while sopD and spvB were observed in 90% and 32.5% of strains, respectively. All strains adhered to and invaded HeLa cells. Regarding biofilm production, 31 (77.5%) strains were able to produce biofilm on polystyrene microplates. Using PFGE, we detected the persistence of clones in the environment for up to 18 from the 20 weeks. The ability of these strains to produce a biofilm and thus persist in the environment and disperse through contact surfaces in the processing plant favors the contamination of food, aggravated by the pathogenic potential of these isolates demonstrated by their adhesion capacity, invasion and resistance to various antibiotic agents. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-10T19:51:18Z 2020-12-10T19:51:18Z 2020-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.foodres.2019.108835 Food Research International. Amsterdam: Elsevier, v. 129, 8 p., 2020. 0963-9969 http://hdl.handle.net/11449/196636 10.1016/j.foodres.2019.108835 WOS:000517665500024 |
url |
http://dx.doi.org/10.1016/j.foodres.2019.108835 http://hdl.handle.net/11449/196636 |
identifier_str_mv |
Food Research International. Amsterdam: Elsevier, v. 129, 8 p., 2020. 0963-9969 10.1016/j.foodres.2019.108835 WOS:000517665500024 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Food Research International |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
8 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965297677959168 |