Atlantic Salmon (Salmo salar) bacterial and viral innate immune responses are not impaired by florfenicol or tetracycline administration
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.fsi.2022.02.034 http://hdl.handle.net/11449/237728 |
Resumo: | Antibiotics are used to treat bacterial infections in fish aquaculture, and these drugs can interact with immune cells/the immune system and potentially leave fish vulnerable to viral, fungal, parasitic, or other bacterial infections. However, the effects of antibiotics on fish immunity have largely been overlooked by the aquaculture industry. We tested, at 12 and 20 degrees C, whether tetracycline and florfenicol (the most commonly used antibiotics in commercial aquaculture), affected the Atlantic salmon's capacity to respond to bacterial or viral stimulation. Atlantic salmon were acclimated to 12 or 20 degrees C and fed with tetracycline or florfenicol (100 and 10 mg kg of body weight-1 day(-1), respectively) medicated feed for 15 or 10 days, respectively. Thereafter, we evaluated their immune function prior to, and after, an intraperitoneal injection of Forte Micro (containing inactivated cultures of Aeromonas salmonicida, Vibrio anguillarum, Vibrio ordalii and Vibrio salmonicida) or the viral mimic polyriboinosinic polyribocytidylic acid (pIC). We measured the transcript expression levels of 8 anti-bacterial and 8 anti-viral putative biomarker genes, and the innate (leukocyte respiratory burst, plasma lysozyme activity and hemolytic activity of the alternative complement pathway) and cellular (relative number of erythrocytes, lymphocytes and thrombocytes, and granulocytes such as monocytes and neutrophils) responses to these challenges. Overall, we only found a few minor effects of either tetracycline or florfenicol on immune gene expression or function at either temperature. Although several studies have reported that antibiotics may negatively affect fish immune responses, our results show that industry-relevant dietary tetracycline and florfenicol treatments do not substantially impact the salmon's innate immune responses. Currently, this is the most comprehensive study on the effects of antibiotics administrated according to industry protocols on immune function in Atlantic salmon. |
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Atlantic Salmon (Salmo salar) bacterial and viral innate immune responses are not impaired by florfenicol or tetracycline administrationTemperatureImmunityAntibiotic treatmentClimate changeGlobal warmingSalmon aquacultureAntibiotics are used to treat bacterial infections in fish aquaculture, and these drugs can interact with immune cells/the immune system and potentially leave fish vulnerable to viral, fungal, parasitic, or other bacterial infections. However, the effects of antibiotics on fish immunity have largely been overlooked by the aquaculture industry. We tested, at 12 and 20 degrees C, whether tetracycline and florfenicol (the most commonly used antibiotics in commercial aquaculture), affected the Atlantic salmon's capacity to respond to bacterial or viral stimulation. Atlantic salmon were acclimated to 12 or 20 degrees C and fed with tetracycline or florfenicol (100 and 10 mg kg of body weight-1 day(-1), respectively) medicated feed for 15 or 10 days, respectively. Thereafter, we evaluated their immune function prior to, and after, an intraperitoneal injection of Forte Micro (containing inactivated cultures of Aeromonas salmonicida, Vibrio anguillarum, Vibrio ordalii and Vibrio salmonicida) or the viral mimic polyriboinosinic polyribocytidylic acid (pIC). We measured the transcript expression levels of 8 anti-bacterial and 8 anti-viral putative biomarker genes, and the innate (leukocyte respiratory burst, plasma lysozyme activity and hemolytic activity of the alternative complement pathway) and cellular (relative number of erythrocytes, lymphocytes and thrombocytes, and granulocytes such as monocytes and neutrophils) responses to these challenges. Overall, we only found a few minor effects of either tetracycline or florfenicol on immune gene expression or function at either temperature. Although several studies have reported that antibiotics may negatively affect fish immune responses, our results show that industry-relevant dietary tetracycline and florfenicol treatments do not substantially impact the salmon's innate immune responses. Currently, this is the most comprehensive study on the effects of antibiotics administrated according to industry protocols on immune function in Atlantic salmon.Ocean Frontier Institute through an award from the Canada First Research Excellence FundMem Univ Newfoundland & Labrador, Dept Ocean Sci, St John, NF A1C 5S7, CanadaMem Univ Newfoundland & Labrador, Ocean Sci Ctr, CREAIT Network, Aquat Res Cluster, St John, NF A1C 5S7, CanadaSao Paulo State Univ Unesp, Aquaculture Ctr Unesp, Via Acesso Prof Paulo Donato Castellane, BR-14884900 Jaboticabal, SP, BrazilSao Paulo State Univ Unesp, Aquaculture Ctr Unesp, Via Acesso Prof Paulo Donato Castellane, BR-14884900 Jaboticabal, SP, Brazil: 6604Elsevier B.V.Mem Univ Newfoundland & LabradorUniversidade Estadual Paulista (UNESP)Zanuzzo, Fabio S. [UNESP]Sandrelli, Rebeccah M.Peroni, Ellen de Fatima C.Hall, Jennifer R.Rise, Matthew L.Gamperl, Anthony K.2022-11-30T13:43:04Z2022-11-30T13:43:04Z2022-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article298-313http://dx.doi.org/10.1016/j.fsi.2022.02.034Fish & Shellfish Immunology. London: Academic Press Ltd- Elsevier Science Ltd, v. 123, p. 298-313, 2022.1050-4648http://hdl.handle.net/11449/23772810.1016/j.fsi.2022.02.034WOS:000792809100004Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFish & Shellfish Immunologyinfo:eu-repo/semantics/openAccess2024-04-09T15:10:26Zoai:repositorio.unesp.br:11449/237728Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T13:58:46.187109Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Atlantic Salmon (Salmo salar) bacterial and viral innate immune responses are not impaired by florfenicol or tetracycline administration |
title |
Atlantic Salmon (Salmo salar) bacterial and viral innate immune responses are not impaired by florfenicol or tetracycline administration |
spellingShingle |
Atlantic Salmon (Salmo salar) bacterial and viral innate immune responses are not impaired by florfenicol or tetracycline administration Zanuzzo, Fabio S. [UNESP] Temperature Immunity Antibiotic treatment Climate change Global warming Salmon aquaculture |
title_short |
Atlantic Salmon (Salmo salar) bacterial and viral innate immune responses are not impaired by florfenicol or tetracycline administration |
title_full |
Atlantic Salmon (Salmo salar) bacterial and viral innate immune responses are not impaired by florfenicol or tetracycline administration |
title_fullStr |
Atlantic Salmon (Salmo salar) bacterial and viral innate immune responses are not impaired by florfenicol or tetracycline administration |
title_full_unstemmed |
Atlantic Salmon (Salmo salar) bacterial and viral innate immune responses are not impaired by florfenicol or tetracycline administration |
title_sort |
Atlantic Salmon (Salmo salar) bacterial and viral innate immune responses are not impaired by florfenicol or tetracycline administration |
author |
Zanuzzo, Fabio S. [UNESP] |
author_facet |
Zanuzzo, Fabio S. [UNESP] Sandrelli, Rebeccah M. Peroni, Ellen de Fatima C. Hall, Jennifer R. Rise, Matthew L. Gamperl, Anthony K. |
author_role |
author |
author2 |
Sandrelli, Rebeccah M. Peroni, Ellen de Fatima C. Hall, Jennifer R. Rise, Matthew L. Gamperl, Anthony K. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Mem Univ Newfoundland & Labrador Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Zanuzzo, Fabio S. [UNESP] Sandrelli, Rebeccah M. Peroni, Ellen de Fatima C. Hall, Jennifer R. Rise, Matthew L. Gamperl, Anthony K. |
dc.subject.por.fl_str_mv |
Temperature Immunity Antibiotic treatment Climate change Global warming Salmon aquaculture |
topic |
Temperature Immunity Antibiotic treatment Climate change Global warming Salmon aquaculture |
description |
Antibiotics are used to treat bacterial infections in fish aquaculture, and these drugs can interact with immune cells/the immune system and potentially leave fish vulnerable to viral, fungal, parasitic, or other bacterial infections. However, the effects of antibiotics on fish immunity have largely been overlooked by the aquaculture industry. We tested, at 12 and 20 degrees C, whether tetracycline and florfenicol (the most commonly used antibiotics in commercial aquaculture), affected the Atlantic salmon's capacity to respond to bacterial or viral stimulation. Atlantic salmon were acclimated to 12 or 20 degrees C and fed with tetracycline or florfenicol (100 and 10 mg kg of body weight-1 day(-1), respectively) medicated feed for 15 or 10 days, respectively. Thereafter, we evaluated their immune function prior to, and after, an intraperitoneal injection of Forte Micro (containing inactivated cultures of Aeromonas salmonicida, Vibrio anguillarum, Vibrio ordalii and Vibrio salmonicida) or the viral mimic polyriboinosinic polyribocytidylic acid (pIC). We measured the transcript expression levels of 8 anti-bacterial and 8 anti-viral putative biomarker genes, and the innate (leukocyte respiratory burst, plasma lysozyme activity and hemolytic activity of the alternative complement pathway) and cellular (relative number of erythrocytes, lymphocytes and thrombocytes, and granulocytes such as monocytes and neutrophils) responses to these challenges. Overall, we only found a few minor effects of either tetracycline or florfenicol on immune gene expression or function at either temperature. Although several studies have reported that antibiotics may negatively affect fish immune responses, our results show that industry-relevant dietary tetracycline and florfenicol treatments do not substantially impact the salmon's innate immune responses. Currently, this is the most comprehensive study on the effects of antibiotics administrated according to industry protocols on immune function in Atlantic salmon. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-30T13:43:04Z 2022-11-30T13:43:04Z 2022-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.fsi.2022.02.034 Fish & Shellfish Immunology. London: Academic Press Ltd- Elsevier Science Ltd, v. 123, p. 298-313, 2022. 1050-4648 http://hdl.handle.net/11449/237728 10.1016/j.fsi.2022.02.034 WOS:000792809100004 |
url |
http://dx.doi.org/10.1016/j.fsi.2022.02.034 http://hdl.handle.net/11449/237728 |
identifier_str_mv |
Fish & Shellfish Immunology. London: Academic Press Ltd- Elsevier Science Ltd, v. 123, p. 298-313, 2022. 1050-4648 10.1016/j.fsi.2022.02.034 WOS:000792809100004 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Fish & Shellfish Immunology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
298-313 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128218136313856 |