A trypsin inhibitor from Moringa oleifera flower extract is cytotoxic to Trypanosoma cruzi with high selectivity over mammalian cells
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UNESP |
| Texto Completo: | http://dx.doi.org/10.1080/14786419.2017.1389932 http://hdl.handle.net/11449/185484 |
Resumo: | In this study, Moringa oleifera flower extract and a trypsin inhibitor (MoFTI) isolated from it were evaluated for anti-protozoal activity against Trypanosoma cruzi and cytotoxicity to mammalian cells. The presence of flavonoids was remarkable in the HPLC fingerprints of the extract at 254 and 360 nm. Amino acid sequences of peptides derived from in-gel digestion of MoFTI were determined. Both the extract and MoFTI caused lysis of T. cruzi trypomastigotes with LC50/24 h of 54.18 +/- 6.62 and 41.20 +/- 4.28 mu g/mL, respectively. High selectivity indices (7.9 to >12) for T. cruzi cells over murine peritoneal macrophages and Vero cells were found for the extract and MoFTI. The results show that MoFTI is a trypanocidal principle of the flower extract. [GRAPHICS] . |
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A trypsin inhibitor from Moringa oleifera flower extract is cytotoxic to Trypanosoma cruzi with high selectivity over mammalian cellsMoringa oleiferachagas diseaseprotease inhibitortrypomastigotecytotoxicityIn this study, Moringa oleifera flower extract and a trypsin inhibitor (MoFTI) isolated from it were evaluated for anti-protozoal activity against Trypanosoma cruzi and cytotoxicity to mammalian cells. The presence of flavonoids was remarkable in the HPLC fingerprints of the extract at 254 and 360 nm. Amino acid sequences of peptides derived from in-gel digestion of MoFTI were determined. Both the extract and MoFTI caused lysis of T. cruzi trypomastigotes with LC50/24 h of 54.18 +/- 6.62 and 41.20 +/- 4.28 mu g/mL, respectively. High selectivity indices (7.9 to >12) for T. cruzi cells over murine peritoneal macrophages and Vero cells were found for the extract and MoFTI. The results show that MoFTI is a trypanocidal principle of the flower extract. [GRAPHICS] .Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundacao de Amparo a Ciencia e Tecnologia do Estado de Pernambuco (FACEPE)Univ Fed Rural Pernambuco, Dept Morfol & Fisiol Anim, Recife, PE, BrazilFundacao Oswaldo Cruz, Ctr Pesquisas Aggeu Magalhaes, Dept Microbiol, Recife, PE, BrazilUniv Estadual Paulista, Inst Quim, Dept Quim Organ, Araraquara, BrazilUniv Fed Pernambuco, Ctr Ciencias Biol, Dept Bioquim, Recife, PE, BrazilUniv Fed Rio Janeiro, Inst Bioquim Med Leopoldo de Meis, Rio De Janeiro, BrazilUniv Estadual Paulista, Inst Quim, Dept Quim Organ, Araraquara, BrazilCNPq: 472546/2012-0CNPq: 446902/2014-4Fundacao de Amparo a Ciencia e Tecnologia do Estado de Pernambuco (FACEPE): APQ-0108-2.08/14Taylor & Francis LtdUniv Fed Rural PernambucoFundacao Oswaldo CruzUniversidade Estadual Paulista (Unesp)Universidade Federal de Pernambuco (UFPE)Univ Fed Rio JaneiroPontual, Emmanuel V.Pires-Neto, Divar F.Fraige, Karina [UNESP]Higino, Taciana M. M.Carvalho, Belany E. A.Alves, Neyla M. P.Lima, Thamarah A.Zingali, Russolina B.Coelho, Luana C. B. B.Bolzani, Vanderlan S. [UNESP]Figueiredo, Regina C. B. Q.Napoleao, Thiago H.Paiva, Patricia M. G.2019-10-04T12:35:55Z2019-10-04T12:35:55Z2018-12-17info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2940-2944http://dx.doi.org/10.1080/14786419.2017.1389932Natural Product Research. Abingdon: Taylor & Francis Ltd, v. 32, n. 24, p. 2940-2944, 2018.1478-6419http://hdl.handle.net/11449/18548410.1080/14786419.2017.1389932WOS:000459884600012Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengNatural Product Researchinfo:eu-repo/semantics/openAccess2021-10-23T19:23:18Zoai:repositorio.unesp.br:11449/185484Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T19:23:18Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
| dc.title.none.fl_str_mv |
A trypsin inhibitor from Moringa oleifera flower extract is cytotoxic to Trypanosoma cruzi with high selectivity over mammalian cells |
| title |
A trypsin inhibitor from Moringa oleifera flower extract is cytotoxic to Trypanosoma cruzi with high selectivity over mammalian cells |
| spellingShingle |
A trypsin inhibitor from Moringa oleifera flower extract is cytotoxic to Trypanosoma cruzi with high selectivity over mammalian cells Pontual, Emmanuel V. Moringa oleifera chagas disease protease inhibitor trypomastigote cytotoxicity |
| title_short |
A trypsin inhibitor from Moringa oleifera flower extract is cytotoxic to Trypanosoma cruzi with high selectivity over mammalian cells |
| title_full |
A trypsin inhibitor from Moringa oleifera flower extract is cytotoxic to Trypanosoma cruzi with high selectivity over mammalian cells |
| title_fullStr |
A trypsin inhibitor from Moringa oleifera flower extract is cytotoxic to Trypanosoma cruzi with high selectivity over mammalian cells |
| title_full_unstemmed |
A trypsin inhibitor from Moringa oleifera flower extract is cytotoxic to Trypanosoma cruzi with high selectivity over mammalian cells |
| title_sort |
A trypsin inhibitor from Moringa oleifera flower extract is cytotoxic to Trypanosoma cruzi with high selectivity over mammalian cells |
| author |
Pontual, Emmanuel V. |
| author_facet |
Pontual, Emmanuel V. Pires-Neto, Divar F. Fraige, Karina [UNESP] Higino, Taciana M. M. Carvalho, Belany E. A. Alves, Neyla M. P. Lima, Thamarah A. Zingali, Russolina B. Coelho, Luana C. B. B. Bolzani, Vanderlan S. [UNESP] Figueiredo, Regina C. B. Q. Napoleao, Thiago H. Paiva, Patricia M. G. |
| author_role |
author |
| author2 |
Pires-Neto, Divar F. Fraige, Karina [UNESP] Higino, Taciana M. M. Carvalho, Belany E. A. Alves, Neyla M. P. Lima, Thamarah A. Zingali, Russolina B. Coelho, Luana C. B. B. Bolzani, Vanderlan S. [UNESP] Figueiredo, Regina C. B. Q. Napoleao, Thiago H. Paiva, Patricia M. G. |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Univ Fed Rural Pernambuco Fundacao Oswaldo Cruz Universidade Estadual Paulista (Unesp) Universidade Federal de Pernambuco (UFPE) Univ Fed Rio Janeiro |
| dc.contributor.author.fl_str_mv |
Pontual, Emmanuel V. Pires-Neto, Divar F. Fraige, Karina [UNESP] Higino, Taciana M. M. Carvalho, Belany E. A. Alves, Neyla M. P. Lima, Thamarah A. Zingali, Russolina B. Coelho, Luana C. B. B. Bolzani, Vanderlan S. [UNESP] Figueiredo, Regina C. B. Q. Napoleao, Thiago H. Paiva, Patricia M. G. |
| dc.subject.por.fl_str_mv |
Moringa oleifera chagas disease protease inhibitor trypomastigote cytotoxicity |
| topic |
Moringa oleifera chagas disease protease inhibitor trypomastigote cytotoxicity |
| description |
In this study, Moringa oleifera flower extract and a trypsin inhibitor (MoFTI) isolated from it were evaluated for anti-protozoal activity against Trypanosoma cruzi and cytotoxicity to mammalian cells. The presence of flavonoids was remarkable in the HPLC fingerprints of the extract at 254 and 360 nm. Amino acid sequences of peptides derived from in-gel digestion of MoFTI were determined. Both the extract and MoFTI caused lysis of T. cruzi trypomastigotes with LC50/24 h of 54.18 +/- 6.62 and 41.20 +/- 4.28 mu g/mL, respectively. High selectivity indices (7.9 to >12) for T. cruzi cells over murine peritoneal macrophages and Vero cells were found for the extract and MoFTI. The results show that MoFTI is a trypanocidal principle of the flower extract. [GRAPHICS] . |
| publishDate |
2018 |
| dc.date.none.fl_str_mv |
2018-12-17 2019-10-04T12:35:55Z 2019-10-04T12:35:55Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1080/14786419.2017.1389932 Natural Product Research. Abingdon: Taylor & Francis Ltd, v. 32, n. 24, p. 2940-2944, 2018. 1478-6419 http://hdl.handle.net/11449/185484 10.1080/14786419.2017.1389932 WOS:000459884600012 |
| url |
http://dx.doi.org/10.1080/14786419.2017.1389932 http://hdl.handle.net/11449/185484 |
| identifier_str_mv |
Natural Product Research. Abingdon: Taylor & Francis Ltd, v. 32, n. 24, p. 2940-2944, 2018. 1478-6419 10.1080/14786419.2017.1389932 WOS:000459884600012 |
| dc.language.iso.fl_str_mv |
eng |
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eng |
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Natural Product Research |
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info:eu-repo/semantics/openAccess |
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openAccess |
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2940-2944 |
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Taylor & Francis Ltd |
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Taylor & Francis Ltd |
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Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
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Universidade Estadual Paulista (UNESP) |
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UNESP |
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UNESP |
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Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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