Gene and Blood Analysis Reveal That Transfer from Brackish Water to Freshwater Is Less Stressful to the Silverside Odontesthes humensis
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3389/fgene.2018.00028 http://hdl.handle.net/11449/163813 |
Resumo: | Silversides are fish that inhabit marine coastal waters, coastal lagoons, and estuarine regions in southern South America. The freshwater (FW) silversides have the ability to tolerate salinity variations. Odontesthes humensis have similar habitats and biological characteristics of congeneric O. bonariensis, the most studied silverside species and with great economic importance. Studies revealed that O. bonariensis is not fully adapted to FW, despite inhabiting hyposmotic environments in nature. However, there is little information about stressful environments for cultivation of silverside O. humensis. Thus, the aim of this study was to evaluate the stress and osmoregulation responses triggered by the osmotic transfers on silverside O. humensis. Silversides were acclimated to FW (0 ppt) and to brackish water (BW, 10 ppt) and then they were exposed to opposite salinity treatment. Silverside gills and blood were sampled on pre-transfer (D0) and 1, 7, and 15 days (D1, D7, and D15) after changes in environmental salinity, the expression levels of genes atp1a3a, slc12a2b, kcnh1, and hspa1a were determined by quantitative reverse transcription-PCR for evaluation of osmoregulatory and stress responses. Furthermore, glycemia, hematocrit, and osmolality were also evaluated. The expression of atp1a3a was up-and down-regulated at D1 after the FW-BW and BW-FW transfers, respectively. Slc12a2b was up-regulated after FW-BW transfer. Similarly, kcnh1 and hspa1a were up-regulated at D1 after the BW-FW transfer. O. humensis blood osmolality decreased after the exposure to FW. It remained stable after exposure to BW, indicating an efficient hyposmoregulation. The glycemia had a peak at D1 after BW-FW transfer. No changes were observed in hematocrit. The return to the pre-transfer levels at D7 after the significant increases in responses of almost all evaluated molecular and blood parameters indicated that this period is enough for acclimation to the experimental conditions. In conclusion, our results suggest that BW-FW transfer is more stressful to O. humensis than FW-BW transfer and the physiology of O. humensis is only partially adapted to FW. |
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Gene and Blood Analysis Reveal That Transfer from Brackish Water to Freshwater Is Less Stressful to the Silverside Odontesthes humensisacclimationbloodbrackish waterfishfreshwatergenessalttransferSilversides are fish that inhabit marine coastal waters, coastal lagoons, and estuarine regions in southern South America. The freshwater (FW) silversides have the ability to tolerate salinity variations. Odontesthes humensis have similar habitats and biological characteristics of congeneric O. bonariensis, the most studied silverside species and with great economic importance. Studies revealed that O. bonariensis is not fully adapted to FW, despite inhabiting hyposmotic environments in nature. However, there is little information about stressful environments for cultivation of silverside O. humensis. Thus, the aim of this study was to evaluate the stress and osmoregulation responses triggered by the osmotic transfers on silverside O. humensis. Silversides were acclimated to FW (0 ppt) and to brackish water (BW, 10 ppt) and then they were exposed to opposite salinity treatment. Silverside gills and blood were sampled on pre-transfer (D0) and 1, 7, and 15 days (D1, D7, and D15) after changes in environmental salinity, the expression levels of genes atp1a3a, slc12a2b, kcnh1, and hspa1a were determined by quantitative reverse transcription-PCR for evaluation of osmoregulatory and stress responses. Furthermore, glycemia, hematocrit, and osmolality were also evaluated. The expression of atp1a3a was up-and down-regulated at D1 after the FW-BW and BW-FW transfers, respectively. Slc12a2b was up-regulated after FW-BW transfer. Similarly, kcnh1 and hspa1a were up-regulated at D1 after the BW-FW transfer. O. humensis blood osmolality decreased after the exposure to FW. It remained stable after exposure to BW, indicating an efficient hyposmoregulation. The glycemia had a peak at D1 after BW-FW transfer. No changes were observed in hematocrit. The return to the pre-transfer levels at D7 after the significant increases in responses of almost all evaluated molecular and blood parameters indicated that this period is enough for acclimation to the experimental conditions. In conclusion, our results suggest that BW-FW transfer is more stressful to O. humensis than FW-BW transfer and the physiology of O. humensis is only partially adapted to FW.Ministerio da CienciaConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Fed Pelotas, Technol Dev Ctr, Lab Struct Genom, Pelotas, BrazilUniv Fed Pelotas, Inst Biol, Lab Physiol, Pelotas, BrazilUniv Fed Pelotas, Technol Dev Ctr, Lab Canc Biotechnol, Pelotas, BrazilSao Paulo State Univ, Inst Biosci Botucatu, Dept Genet, Genom & Mol Evolut Lab, Botucatu, SP, BrazilUniv Fed Pelotas, Technol Dev Ctr, Lab Vaccinol, Pelotas, BrazilSao Paulo State Univ, Inst Biosci Botucatu, Dept Genet, Genom & Mol Evolut Lab, Botucatu, SP, BrazilMinisterio da Ciencia: 422292/2016-8CNPq: 472210/2013-0CAPES: AUXPE 2900/2014Frontiers Media SaUniv Fed PelotasUniversidade Estadual Paulista (Unesp)Silveira, Tony L. R.Martins, Gabriel B.Domingues, William B.Remiao, Mariana H.Barreto, Bruna F.Lessa, Ingrid M.Santos, LucasPinhal, Danillo [UNESP]Dellagostin, Odir A.Seixas, Fabiana K.Collares, TiagoRobaldo, Ricardo B.Campos, Vinicius F.2018-11-26T17:45:04Z2018-11-26T17:45:04Z2018-02-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10application/pdfhttp://dx.doi.org/10.3389/fgene.2018.00028Frontiers In Genetics. Lausanne: Frontiers Media Sa, v. 9, 10 p., 2018.1664-8021http://hdl.handle.net/11449/16381310.3389/fgene.2018.00028WOS:000424244300002WOS000424244300002.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers In Genetics2,274info:eu-repo/semantics/openAccess2023-12-18T06:17:27Zoai:repositorio.unesp.br:11449/163813Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-18T06:17:27Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Gene and Blood Analysis Reveal That Transfer from Brackish Water to Freshwater Is Less Stressful to the Silverside Odontesthes humensis |
title |
Gene and Blood Analysis Reveal That Transfer from Brackish Water to Freshwater Is Less Stressful to the Silverside Odontesthes humensis |
spellingShingle |
Gene and Blood Analysis Reveal That Transfer from Brackish Water to Freshwater Is Less Stressful to the Silverside Odontesthes humensis Silveira, Tony L. R. acclimation blood brackish water fish freshwater genes salt transfer |
title_short |
Gene and Blood Analysis Reveal That Transfer from Brackish Water to Freshwater Is Less Stressful to the Silverside Odontesthes humensis |
title_full |
Gene and Blood Analysis Reveal That Transfer from Brackish Water to Freshwater Is Less Stressful to the Silverside Odontesthes humensis |
title_fullStr |
Gene and Blood Analysis Reveal That Transfer from Brackish Water to Freshwater Is Less Stressful to the Silverside Odontesthes humensis |
title_full_unstemmed |
Gene and Blood Analysis Reveal That Transfer from Brackish Water to Freshwater Is Less Stressful to the Silverside Odontesthes humensis |
title_sort |
Gene and Blood Analysis Reveal That Transfer from Brackish Water to Freshwater Is Less Stressful to the Silverside Odontesthes humensis |
author |
Silveira, Tony L. R. |
author_facet |
Silveira, Tony L. R. Martins, Gabriel B. Domingues, William B. Remiao, Mariana H. Barreto, Bruna F. Lessa, Ingrid M. Santos, Lucas Pinhal, Danillo [UNESP] Dellagostin, Odir A. Seixas, Fabiana K. Collares, Tiago Robaldo, Ricardo B. Campos, Vinicius F. |
author_role |
author |
author2 |
Martins, Gabriel B. Domingues, William B. Remiao, Mariana H. Barreto, Bruna F. Lessa, Ingrid M. Santos, Lucas Pinhal, Danillo [UNESP] Dellagostin, Odir A. Seixas, Fabiana K. Collares, Tiago Robaldo, Ricardo B. Campos, Vinicius F. |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Univ Fed Pelotas Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Silveira, Tony L. R. Martins, Gabriel B. Domingues, William B. Remiao, Mariana H. Barreto, Bruna F. Lessa, Ingrid M. Santos, Lucas Pinhal, Danillo [UNESP] Dellagostin, Odir A. Seixas, Fabiana K. Collares, Tiago Robaldo, Ricardo B. Campos, Vinicius F. |
dc.subject.por.fl_str_mv |
acclimation blood brackish water fish freshwater genes salt transfer |
topic |
acclimation blood brackish water fish freshwater genes salt transfer |
description |
Silversides are fish that inhabit marine coastal waters, coastal lagoons, and estuarine regions in southern South America. The freshwater (FW) silversides have the ability to tolerate salinity variations. Odontesthes humensis have similar habitats and biological characteristics of congeneric O. bonariensis, the most studied silverside species and with great economic importance. Studies revealed that O. bonariensis is not fully adapted to FW, despite inhabiting hyposmotic environments in nature. However, there is little information about stressful environments for cultivation of silverside O. humensis. Thus, the aim of this study was to evaluate the stress and osmoregulation responses triggered by the osmotic transfers on silverside O. humensis. Silversides were acclimated to FW (0 ppt) and to brackish water (BW, 10 ppt) and then they were exposed to opposite salinity treatment. Silverside gills and blood were sampled on pre-transfer (D0) and 1, 7, and 15 days (D1, D7, and D15) after changes in environmental salinity, the expression levels of genes atp1a3a, slc12a2b, kcnh1, and hspa1a were determined by quantitative reverse transcription-PCR for evaluation of osmoregulatory and stress responses. Furthermore, glycemia, hematocrit, and osmolality were also evaluated. The expression of atp1a3a was up-and down-regulated at D1 after the FW-BW and BW-FW transfers, respectively. Slc12a2b was up-regulated after FW-BW transfer. Similarly, kcnh1 and hspa1a were up-regulated at D1 after the BW-FW transfer. O. humensis blood osmolality decreased after the exposure to FW. It remained stable after exposure to BW, indicating an efficient hyposmoregulation. The glycemia had a peak at D1 after BW-FW transfer. No changes were observed in hematocrit. The return to the pre-transfer levels at D7 after the significant increases in responses of almost all evaluated molecular and blood parameters indicated that this period is enough for acclimation to the experimental conditions. In conclusion, our results suggest that BW-FW transfer is more stressful to O. humensis than FW-BW transfer and the physiology of O. humensis is only partially adapted to FW. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-11-26T17:45:04Z 2018-11-26T17:45:04Z 2018-02-06 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3389/fgene.2018.00028 Frontiers In Genetics. Lausanne: Frontiers Media Sa, v. 9, 10 p., 2018. 1664-8021 http://hdl.handle.net/11449/163813 10.3389/fgene.2018.00028 WOS:000424244300002 WOS000424244300002.pdf |
url |
http://dx.doi.org/10.3389/fgene.2018.00028 http://hdl.handle.net/11449/163813 |
identifier_str_mv |
Frontiers In Genetics. Lausanne: Frontiers Media Sa, v. 9, 10 p., 2018. 1664-8021 10.3389/fgene.2018.00028 WOS:000424244300002 WOS000424244300002.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Frontiers In Genetics 2,274 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
10 application/pdf |
dc.publisher.none.fl_str_mv |
Frontiers Media Sa |
publisher.none.fl_str_mv |
Frontiers Media Sa |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799965326982512640 |