Regenerated cellulose scaffolds: Preparation, characterization and toxicological evaluation
Autor(a) principal: | |
---|---|
Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.carbpol.2015.09.066 http://hdl.handle.net/11449/177536 |
Resumo: | Regenerated cellulose scaffolds (RCS) may be used as alloplastic materials for tissue repair. In this work, the RCS were obtained by viscose process and characterized by scanning electron microscopy (SEM), wide angle X-ray diffraction (WAXD), Fourier transform infrared spectroscopy (FTIR) and thermogravimetry analysis (TG). In vitro enzymatic degradation assay and toxicological assays were also evaluated. The physicochemical characterizations revealed the formation of a porous material with distinct thermal profile and crystallinity compared to pristine cellulose pulp. Enzymatic degradation assay revealed that lysozyme showed a mildest catalytic action when compared to cellulase, Tricoderma reesei (Tr). Nevertheless, both enzymes were efficient for degrading the RCS. RCS did not show cytotoxicity, mutagenic or genotoxic effects. The systematically characterization of this work suggests that RCS presented distinct features that make it a viable material for future studies related to the development of scaffolds for biological applications. |
id |
UNSP_63a9a5981e374b8bbaf4e6b639a4bb42 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/177536 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Regenerated cellulose scaffolds: Preparation, characterization and toxicological evaluationCytotoxicityGenotoxicityMutagenicityRegenerated celluloseScaffoldTissue repairRegenerated cellulose scaffolds (RCS) may be used as alloplastic materials for tissue repair. In this work, the RCS were obtained by viscose process and characterized by scanning electron microscopy (SEM), wide angle X-ray diffraction (WAXD), Fourier transform infrared spectroscopy (FTIR) and thermogravimetry analysis (TG). In vitro enzymatic degradation assay and toxicological assays were also evaluated. The physicochemical characterizations revealed the formation of a porous material with distinct thermal profile and crystallinity compared to pristine cellulose pulp. Enzymatic degradation assay revealed that lysozyme showed a mildest catalytic action when compared to cellulase, Tricoderma reesei (Tr). Nevertheless, both enzymes were efficient for degrading the RCS. RCS did not show cytotoxicity, mutagenic or genotoxic effects. The systematically characterization of this work suggests that RCS presented distinct features that make it a viable material for future studies related to the development of scaffolds for biological applications.Institute of Chemistry, São Paulo State University, UNESP, PO Box 355Dental School at Araraquara, São Paulo State University, UNESP, Rua Humaitá, 1680Laboratório de Química Medicinal e Medinica Regenerativa (QUIMMERA), Centro Universitário de Araraquara (UNIARA)Ribeirão Preto Medical School, University of São Paulo, USP, Av. BandeirantesFaculdade de Ciências Integradas Do Pontal, Universidade Federal de Uberlândia, Campus Do Pontal, Rua Vinte, 1600Institute of Chemistry, São Paulo State University, UNESP, PO Box 355Dental School at Araraquara, São Paulo State University, UNESP, Rua Humaitá, 1680Universidade Estadual Paulista (Unesp)Laboratório de Química Medicinal e Medinica Regenerativa (QUIMMERA), Centro Universitário de Araraquara (UNIARA)Universidade de São Paulo (USP)Universidade Federal de Uberlândia (UFU)De Araújo Júnior, Adalberto M. [UNESP]Braido, Guilherme [UNESP]Saska, Sybele [UNESP]Barud, Hernane S. [UNESP]Franchi, Leonardo P.Assunção, Rosana M.N.Scarel-Caminaga, Raquel M. [UNESP]Capote, Ticiana S.O. [UNESP]Messaddeq, Younès [UNESP]Ribeiro, Sidney J.L. [UNESP]2018-12-11T17:25:53Z2018-12-11T17:25:53Z2016-01-20info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article892-898application/pdfhttp://dx.doi.org/10.1016/j.carbpol.2015.09.066Carbohydrate Polymers, v. 136, p. 892-898.0144-8617http://hdl.handle.net/11449/17753610.1016/j.carbpol.2015.09.0662-s2.0-849438127822-s2.0-84943812782.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCarbohydrate Polymers1,428info:eu-repo/semantics/openAccess2023-11-29T06:15:55Zoai:repositorio.unesp.br:11449/177536Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:04:42.179493Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Regenerated cellulose scaffolds: Preparation, characterization and toxicological evaluation |
title |
Regenerated cellulose scaffolds: Preparation, characterization and toxicological evaluation |
spellingShingle |
Regenerated cellulose scaffolds: Preparation, characterization and toxicological evaluation De Araújo Júnior, Adalberto M. [UNESP] Cytotoxicity Genotoxicity Mutagenicity Regenerated cellulose Scaffold Tissue repair |
title_short |
Regenerated cellulose scaffolds: Preparation, characterization and toxicological evaluation |
title_full |
Regenerated cellulose scaffolds: Preparation, characterization and toxicological evaluation |
title_fullStr |
Regenerated cellulose scaffolds: Preparation, characterization and toxicological evaluation |
title_full_unstemmed |
Regenerated cellulose scaffolds: Preparation, characterization and toxicological evaluation |
title_sort |
Regenerated cellulose scaffolds: Preparation, characterization and toxicological evaluation |
author |
De Araújo Júnior, Adalberto M. [UNESP] |
author_facet |
De Araújo Júnior, Adalberto M. [UNESP] Braido, Guilherme [UNESP] Saska, Sybele [UNESP] Barud, Hernane S. [UNESP] Franchi, Leonardo P. Assunção, Rosana M.N. Scarel-Caminaga, Raquel M. [UNESP] Capote, Ticiana S.O. [UNESP] Messaddeq, Younès [UNESP] Ribeiro, Sidney J.L. [UNESP] |
author_role |
author |
author2 |
Braido, Guilherme [UNESP] Saska, Sybele [UNESP] Barud, Hernane S. [UNESP] Franchi, Leonardo P. Assunção, Rosana M.N. Scarel-Caminaga, Raquel M. [UNESP] Capote, Ticiana S.O. [UNESP] Messaddeq, Younès [UNESP] Ribeiro, Sidney J.L. [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Laboratório de Química Medicinal e Medinica Regenerativa (QUIMMERA), Centro Universitário de Araraquara (UNIARA) Universidade de São Paulo (USP) Universidade Federal de Uberlândia (UFU) |
dc.contributor.author.fl_str_mv |
De Araújo Júnior, Adalberto M. [UNESP] Braido, Guilherme [UNESP] Saska, Sybele [UNESP] Barud, Hernane S. [UNESP] Franchi, Leonardo P. Assunção, Rosana M.N. Scarel-Caminaga, Raquel M. [UNESP] Capote, Ticiana S.O. [UNESP] Messaddeq, Younès [UNESP] Ribeiro, Sidney J.L. [UNESP] |
dc.subject.por.fl_str_mv |
Cytotoxicity Genotoxicity Mutagenicity Regenerated cellulose Scaffold Tissue repair |
topic |
Cytotoxicity Genotoxicity Mutagenicity Regenerated cellulose Scaffold Tissue repair |
description |
Regenerated cellulose scaffolds (RCS) may be used as alloplastic materials for tissue repair. In this work, the RCS were obtained by viscose process and characterized by scanning electron microscopy (SEM), wide angle X-ray diffraction (WAXD), Fourier transform infrared spectroscopy (FTIR) and thermogravimetry analysis (TG). In vitro enzymatic degradation assay and toxicological assays were also evaluated. The physicochemical characterizations revealed the formation of a porous material with distinct thermal profile and crystallinity compared to pristine cellulose pulp. Enzymatic degradation assay revealed that lysozyme showed a mildest catalytic action when compared to cellulase, Tricoderma reesei (Tr). Nevertheless, both enzymes were efficient for degrading the RCS. RCS did not show cytotoxicity, mutagenic or genotoxic effects. The systematically characterization of this work suggests that RCS presented distinct features that make it a viable material for future studies related to the development of scaffolds for biological applications. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-01-20 2018-12-11T17:25:53Z 2018-12-11T17:25:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.carbpol.2015.09.066 Carbohydrate Polymers, v. 136, p. 892-898. 0144-8617 http://hdl.handle.net/11449/177536 10.1016/j.carbpol.2015.09.066 2-s2.0-84943812782 2-s2.0-84943812782.pdf |
url |
http://dx.doi.org/10.1016/j.carbpol.2015.09.066 http://hdl.handle.net/11449/177536 |
identifier_str_mv |
Carbohydrate Polymers, v. 136, p. 892-898. 0144-8617 10.1016/j.carbpol.2015.09.066 2-s2.0-84943812782 2-s2.0-84943812782.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Carbohydrate Polymers 1,428 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
892-898 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129016516837376 |