Linear Mitochondrial Genome in Anthozoa (Cnidaria): A Case Study in Ceriantharia
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1038/s41598-019-42621-z http://hdl.handle.net/11449/187565 |
Resumo: | Sequences and structural attributes of mitochondrial genomes have played a critical role in the clarification of relationships among Cnidaria, a key phylum of early-diverging animals. Among the major lineages of Cnidaria, Ceriantharia (“tube anemones”) remains one of the most enigmatic in terms of its phylogenetic position. We sequenced the mitochondrial genomes of two ceriantharians to see whether the complete organellar genome would provide more support for the phylogenetic placement of Ceriantharia. For both Isarachnanthus nocturnus and Pachycerianthus magnus, the mitochondrial gene sequences could not be assembled into a single circular genome. Instead, our analyses suggest that both species have mitochondrial genomes consisting of multiple linear fragments. Linear mitogenomes are characteristic of members of Medusozoa, one of the major lineages of Cnidaria, but are unreported for Anthozoa, which includes the Ceriantharia. The inferred number of fragments and variation in gene order between species is much greater within Ceriantharia than among the lineages of Medusozoa. We identify origins of replication for each of the five putative chromosomes of the Isarachnanthus nocturnus mitogenome and for each of the eight putative chromosomes of the Pachycerianthus magnus mitogenome. At 80,923 bp, I. nocturnus now holds the record for the largest animal mitochondrial genome reported to date. The novelty of the mitogenomic structure in Ceriantharia highlights the distinctiveness of this lineage but, because it appears to be both unique to and diverse within Ceriantharia, it is uninformative about the phylogenetic position of Ceriantharia relative to other Anthozoa. The presence of tRNA Met and tRNA Trp in both ceriantharian mitogenomes supports a closer relationship between Ceriantharia and Hexacorallia than between Ceriantharia and any other cnidarian lineage, but phylogenetic analysis of the genes contained in the mitogenomes suggests that Ceriantharia is sister to a clade containing Octocorallia + Hexacorallia indicating a possible suppression of tRNA Trp in Octocorallia. |
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Linear Mitochondrial Genome in Anthozoa (Cnidaria): A Case Study in CerianthariaSequences and structural attributes of mitochondrial genomes have played a critical role in the clarification of relationships among Cnidaria, a key phylum of early-diverging animals. Among the major lineages of Cnidaria, Ceriantharia (“tube anemones”) remains one of the most enigmatic in terms of its phylogenetic position. We sequenced the mitochondrial genomes of two ceriantharians to see whether the complete organellar genome would provide more support for the phylogenetic placement of Ceriantharia. For both Isarachnanthus nocturnus and Pachycerianthus magnus, the mitochondrial gene sequences could not be assembled into a single circular genome. Instead, our analyses suggest that both species have mitochondrial genomes consisting of multiple linear fragments. Linear mitogenomes are characteristic of members of Medusozoa, one of the major lineages of Cnidaria, but are unreported for Anthozoa, which includes the Ceriantharia. The inferred number of fragments and variation in gene order between species is much greater within Ceriantharia than among the lineages of Medusozoa. We identify origins of replication for each of the five putative chromosomes of the Isarachnanthus nocturnus mitogenome and for each of the eight putative chromosomes of the Pachycerianthus magnus mitogenome. At 80,923 bp, I. nocturnus now holds the record for the largest animal mitochondrial genome reported to date. The novelty of the mitogenomic structure in Ceriantharia highlights the distinctiveness of this lineage but, because it appears to be both unique to and diverse within Ceriantharia, it is uninformative about the phylogenetic position of Ceriantharia relative to other Anthozoa. The presence of tRNA Met and tRNA Trp in both ceriantharian mitogenomes supports a closer relationship between Ceriantharia and Hexacorallia than between Ceriantharia and any other cnidarian lineage, but phylogenetic analysis of the genes contained in the mitogenomes suggests that Ceriantharia is sister to a clade containing Octocorallia + Hexacorallia indicating a possible suppression of tRNA Trp in Octocorallia.National Science FoundationDepartamento de Ciências Biológicas Faculdade de Ciências e Letras UNESP – Universidade Estadual PaulistaDepartment of Evolution Ecology and Organismal Biology The Ohio State UniversityDepartment of Biological Sciences University of North Carolina at CharlotteDepartment of Biology Florida Southern CollegeBiological Sciences Department NYC College of Technology City University of New York, 285 Jay StreetDepartment of Invertebrate Zoology American Museum of Natural History, Central Park West at 79th StreetDepartamento de Ciências Biológicas Faculdade de Ciências e Letras UNESP – Universidade Estadual PaulistaNational Science Foundation: DEB-1257796Universidade Estadual Paulista (Unesp)The Ohio State UniversityUniversity of North Carolina at CharlotteFlorida Southern CollegeCity University of New YorkAmerican Museum of Natural HistoryStampar, Sérgio N. [UNESP]Broe, Michael B.Macrander, JasonReitzel, Adam M.Brugler, Mercer R.Daly, Marymegan2019-10-06T15:40:18Z2019-10-06T15:40:18Z2019-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1038/s41598-019-42621-zScientific Reports, v. 9, n. 1, 2019.2045-2322http://hdl.handle.net/11449/18756510.1038/s41598-019-42621-z2-s2.0-85064466081Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientific Reportsinfo:eu-repo/semantics/openAccess2021-10-23T11:59:48Zoai:repositorio.unesp.br:11449/187565Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:18:14.659578Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Linear Mitochondrial Genome in Anthozoa (Cnidaria): A Case Study in Ceriantharia |
title |
Linear Mitochondrial Genome in Anthozoa (Cnidaria): A Case Study in Ceriantharia |
spellingShingle |
Linear Mitochondrial Genome in Anthozoa (Cnidaria): A Case Study in Ceriantharia Stampar, Sérgio N. [UNESP] |
title_short |
Linear Mitochondrial Genome in Anthozoa (Cnidaria): A Case Study in Ceriantharia |
title_full |
Linear Mitochondrial Genome in Anthozoa (Cnidaria): A Case Study in Ceriantharia |
title_fullStr |
Linear Mitochondrial Genome in Anthozoa (Cnidaria): A Case Study in Ceriantharia |
title_full_unstemmed |
Linear Mitochondrial Genome in Anthozoa (Cnidaria): A Case Study in Ceriantharia |
title_sort |
Linear Mitochondrial Genome in Anthozoa (Cnidaria): A Case Study in Ceriantharia |
author |
Stampar, Sérgio N. [UNESP] |
author_facet |
Stampar, Sérgio N. [UNESP] Broe, Michael B. Macrander, Jason Reitzel, Adam M. Brugler, Mercer R. Daly, Marymegan |
author_role |
author |
author2 |
Broe, Michael B. Macrander, Jason Reitzel, Adam M. Brugler, Mercer R. Daly, Marymegan |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) The Ohio State University University of North Carolina at Charlotte Florida Southern College City University of New York American Museum of Natural History |
dc.contributor.author.fl_str_mv |
Stampar, Sérgio N. [UNESP] Broe, Michael B. Macrander, Jason Reitzel, Adam M. Brugler, Mercer R. Daly, Marymegan |
description |
Sequences and structural attributes of mitochondrial genomes have played a critical role in the clarification of relationships among Cnidaria, a key phylum of early-diverging animals. Among the major lineages of Cnidaria, Ceriantharia (“tube anemones”) remains one of the most enigmatic in terms of its phylogenetic position. We sequenced the mitochondrial genomes of two ceriantharians to see whether the complete organellar genome would provide more support for the phylogenetic placement of Ceriantharia. For both Isarachnanthus nocturnus and Pachycerianthus magnus, the mitochondrial gene sequences could not be assembled into a single circular genome. Instead, our analyses suggest that both species have mitochondrial genomes consisting of multiple linear fragments. Linear mitogenomes are characteristic of members of Medusozoa, one of the major lineages of Cnidaria, but are unreported for Anthozoa, which includes the Ceriantharia. The inferred number of fragments and variation in gene order between species is much greater within Ceriantharia than among the lineages of Medusozoa. We identify origins of replication for each of the five putative chromosomes of the Isarachnanthus nocturnus mitogenome and for each of the eight putative chromosomes of the Pachycerianthus magnus mitogenome. At 80,923 bp, I. nocturnus now holds the record for the largest animal mitochondrial genome reported to date. The novelty of the mitogenomic structure in Ceriantharia highlights the distinctiveness of this lineage but, because it appears to be both unique to and diverse within Ceriantharia, it is uninformative about the phylogenetic position of Ceriantharia relative to other Anthozoa. The presence of tRNA Met and tRNA Trp in both ceriantharian mitogenomes supports a closer relationship between Ceriantharia and Hexacorallia than between Ceriantharia and any other cnidarian lineage, but phylogenetic analysis of the genes contained in the mitogenomes suggests that Ceriantharia is sister to a clade containing Octocorallia + Hexacorallia indicating a possible suppression of tRNA Trp in Octocorallia. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T15:40:18Z 2019-10-06T15:40:18Z 2019-12-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1038/s41598-019-42621-z Scientific Reports, v. 9, n. 1, 2019. 2045-2322 http://hdl.handle.net/11449/187565 10.1038/s41598-019-42621-z 2-s2.0-85064466081 |
url |
http://dx.doi.org/10.1038/s41598-019-42621-z http://hdl.handle.net/11449/187565 |
identifier_str_mv |
Scientific Reports, v. 9, n. 1, 2019. 2045-2322 10.1038/s41598-019-42621-z 2-s2.0-85064466081 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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Scientific Reports |
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info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
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Universidade Estadual Paulista (UNESP) |
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UNESP |
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UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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1808129049206194176 |