Biodisponibilidade de flavanonas e atividade antioxidante do suco de laranja fresco versus suco de laranja pasteurizado em humanos saudáveis

• Autor(a) principal: Silveira, Jacqueline Queiroz da [UNESP]
• Data de Publicação: 2014
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/114027
• topic: Suco de laranja; Antioxidantes; Pasteurização; Hesperidina; Farmacocinetica; Orange juice

Flavanones (hesperidin and narirutin) present almost exclusively in oranges, are associated with various health benefits. The aim of this study was to analyze the differences in the pharmacokinetic parameters of the flavanones metabolites and antioxidant activity in humans after consumption of two types of orange juice: fresh and pasteurized. Fresh orange juice (FOJ) is extracted with the aid of a squeezer MJ-20 Basic and pasteurized (95°C/ 15s) was provided by Citrosuco Matão SP. Both juices were obtained from Pera Rio oranges from the same batch. Participated 12 men and 12 healthy women, 27 ± 6 years, BMI 24 ± 3 kg/m2, and non-smokers who did not use any hormones or dietary supplements. Volunteers ingested 11.5 ml/kg body weight of fresh orange juice and after washout of 30 days, they ingested the same amount of pasteurized juice. Volunteers were asked to avoid consumption of citrus foods in the preceding three days to the experiment and meals of the day (breakfast, lunch and afternoon snack) were provided to ensure the avoidance of foods rich in flavonoids. Urine was collected during 24 h and the blood was collected from fasting, 3, 4, 5, 6, 7, 8 and 24 h after the consumption of the juice. The metabolites were analyzed in plasma and urine by high-performance liquid chromatography- electrospray ionization- mass spectrometry (HPLC-ESI-MS). The antioxidant activity was evaluated in blood serum by ABTS and TBARS, at fasting, 4, 8 and 24 h. The concentrations of hesperidin and narirutina after the ingestion of commercially pasteurized orange juice were 3.2 and 6.6 times higher than after fresh juice, respectively, and these compounds have been found largely in the insoluble fraction (pellet) in pasteurized juice, while occurred almost entirely in the soluble fraction (supernatant) in fresh juice. The urine chromatographic analysis showed concentrations of conjugated of glucuronic acid and sulfate groups of hesperetin and naringenin ...