Generation and Characterization of a Soybean Line with a Vernonia galamensis Diacylglycerol Acyltransferase-1 Gene and a myo-Inositol 1-Phosphate Synthase Knockout Mutation

Detalhes bibliográficos
Autor(a) principal: AL-Amery, Maythem
Data de Publicação: 2020
Outros Autores: Fowler, Ashley, Unrine, Jason M., Armstrong, Paul, Maghirang, Elizabeth, Su, Kai, de Melo, Julia [UNESP], Yuan, Feng-Jie, Shu, Qing-Yao, Hildebrand, David
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1002/lipd.12253
http://hdl.handle.net/11449/198985
Resumo: Soybean (Glycine max) meal is an important protein source. Soybean meal with lower phytate and oligosaccharides improves meal quality. A single recessive mutation in soybean myo-inositol 1-phosphate synthase (Gm-lpa-TW75-1) confers a seed phenotype with low phytate and increased inorganic phosphate. The mutant was crossed with high oil lines expressing a diacylglycerol acyltransferase1 (DGAT) gene from Vernonia galamensis (VgD). Gm-lpa-TW75-1 X VgD, designated GV, has 21%, and 22% oil and 41% and 43% protein from field and greenhouse seed production, respectively. No significant differences were found in mineral concentrations except for Fe which was 229 μg/g dry mass for GV followed by 174.3 for VgD and 162 for Gm-lpa-TW75-1. Phosphate (Pi) is higher in Gm-lpa-TW75-1 as expected at 5 mg/g, followed by GV at 1.6 mg/g whereas Jack, VgD, and Taiwan75 have about 0.3 mg/g. The Gm-lpa-TW75-1 line has the lowest phytate concentration at 1.4 mg/g followed by GV with 1.8 mg/g compared to Taiwan75, VgD, and Jack with 2.5 mg/g. This work describes a high oil and protein soybean line, GV, with increased Pi and lower phytate which will increase the nutritional value for human and animal feed.
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spelling Generation and Characterization of a Soybean Line with a Vernonia galamensis Diacylglycerol Acyltransferase-1 Gene and a myo-Inositol 1-Phosphate Synthase Knockout MutationGlycine maxHigh oilHigh phosphateHigh proteinLow phytateSoybean (Glycine max) meal is an important protein source. Soybean meal with lower phytate and oligosaccharides improves meal quality. A single recessive mutation in soybean myo-inositol 1-phosphate synthase (Gm-lpa-TW75-1) confers a seed phenotype with low phytate and increased inorganic phosphate. The mutant was crossed with high oil lines expressing a diacylglycerol acyltransferase1 (DGAT) gene from Vernonia galamensis (VgD). Gm-lpa-TW75-1 X VgD, designated GV, has 21%, and 22% oil and 41% and 43% protein from field and greenhouse seed production, respectively. No significant differences were found in mineral concentrations except for Fe which was 229 μg/g dry mass for GV followed by 174.3 for VgD and 162 for Gm-lpa-TW75-1. Phosphate (Pi) is higher in Gm-lpa-TW75-1 as expected at 5 mg/g, followed by GV at 1.6 mg/g whereas Jack, VgD, and Taiwan75 have about 0.3 mg/g. The Gm-lpa-TW75-1 line has the lowest phytate concentration at 1.4 mg/g followed by GV with 1.8 mg/g compared to Taiwan75, VgD, and Jack with 2.5 mg/g. This work describes a high oil and protein soybean line, GV, with increased Pi and lower phytate which will increase the nutritional value for human and animal feed.Department of Biology College of Science for Women University of Baghdad, Karrada, Al-Jadriya, 10071Department of Plant and Soil Sciences University of Kentucky, Plant Science Building, 1405 Veterans DriveDepartment of Animal and Food Sciences University of KentuckyUSDA-ARS SPIERU-CGAHRUNESP Campus Jaboticabal State of Sao Paulo 295 9 de julho street ConchalZhejiang Provincial Key Laboratory for Germplasm Resources Institute of Crop Science Zhejiang UniversityUNESP Campus Jaboticabal State of Sao Paulo 295 9 de julho street ConchalUniversity of BaghdadUniversity of KentuckySPIERU-CGAHRUniversidade Estadual Paulista (Unesp)Zhejiang UniversityAL-Amery, MaythemFowler, AshleyUnrine, Jason M.Armstrong, PaulMaghirang, ElizabethSu, Kaide Melo, Julia [UNESP]Yuan, Feng-JieShu, Qing-YaoHildebrand, David2020-12-12T01:27:33Z2020-12-12T01:27:33Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1002/lipd.12253Lipids.1558-93070024-4201http://hdl.handle.net/11449/19898510.1002/lipd.122532-s2.0-85086442106Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLipidsinfo:eu-repo/semantics/openAccess2021-10-22T21:54:27Zoai:repositorio.unesp.br:11449/198985Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:32:30.879500Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Generation and Characterization of a Soybean Line with a Vernonia galamensis Diacylglycerol Acyltransferase-1 Gene and a myo-Inositol 1-Phosphate Synthase Knockout Mutation
title Generation and Characterization of a Soybean Line with a Vernonia galamensis Diacylglycerol Acyltransferase-1 Gene and a myo-Inositol 1-Phosphate Synthase Knockout Mutation
spellingShingle Generation and Characterization of a Soybean Line with a Vernonia galamensis Diacylglycerol Acyltransferase-1 Gene and a myo-Inositol 1-Phosphate Synthase Knockout Mutation
AL-Amery, Maythem
Glycine max
High oil
High phosphate
High protein
Low phytate
title_short Generation and Characterization of a Soybean Line with a Vernonia galamensis Diacylglycerol Acyltransferase-1 Gene and a myo-Inositol 1-Phosphate Synthase Knockout Mutation
title_full Generation and Characterization of a Soybean Line with a Vernonia galamensis Diacylglycerol Acyltransferase-1 Gene and a myo-Inositol 1-Phosphate Synthase Knockout Mutation
title_fullStr Generation and Characterization of a Soybean Line with a Vernonia galamensis Diacylglycerol Acyltransferase-1 Gene and a myo-Inositol 1-Phosphate Synthase Knockout Mutation
title_full_unstemmed Generation and Characterization of a Soybean Line with a Vernonia galamensis Diacylglycerol Acyltransferase-1 Gene and a myo-Inositol 1-Phosphate Synthase Knockout Mutation
title_sort Generation and Characterization of a Soybean Line with a Vernonia galamensis Diacylglycerol Acyltransferase-1 Gene and a myo-Inositol 1-Phosphate Synthase Knockout Mutation
author AL-Amery, Maythem
author_facet AL-Amery, Maythem
Fowler, Ashley
Unrine, Jason M.
Armstrong, Paul
Maghirang, Elizabeth
Su, Kai
de Melo, Julia [UNESP]
Yuan, Feng-Jie
Shu, Qing-Yao
Hildebrand, David
author_role author
author2 Fowler, Ashley
Unrine, Jason M.
Armstrong, Paul
Maghirang, Elizabeth
Su, Kai
de Melo, Julia [UNESP]
Yuan, Feng-Jie
Shu, Qing-Yao
Hildebrand, David
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv University of Baghdad
University of Kentucky
SPIERU-CGAHR
Universidade Estadual Paulista (Unesp)
Zhejiang University
dc.contributor.author.fl_str_mv AL-Amery, Maythem
Fowler, Ashley
Unrine, Jason M.
Armstrong, Paul
Maghirang, Elizabeth
Su, Kai
de Melo, Julia [UNESP]
Yuan, Feng-Jie
Shu, Qing-Yao
Hildebrand, David
dc.subject.por.fl_str_mv Glycine max
High oil
High phosphate
High protein
Low phytate
topic Glycine max
High oil
High phosphate
High protein
Low phytate
description Soybean (Glycine max) meal is an important protein source. Soybean meal with lower phytate and oligosaccharides improves meal quality. A single recessive mutation in soybean myo-inositol 1-phosphate synthase (Gm-lpa-TW75-1) confers a seed phenotype with low phytate and increased inorganic phosphate. The mutant was crossed with high oil lines expressing a diacylglycerol acyltransferase1 (DGAT) gene from Vernonia galamensis (VgD). Gm-lpa-TW75-1 X VgD, designated GV, has 21%, and 22% oil and 41% and 43% protein from field and greenhouse seed production, respectively. No significant differences were found in mineral concentrations except for Fe which was 229 μg/g dry mass for GV followed by 174.3 for VgD and 162 for Gm-lpa-TW75-1. Phosphate (Pi) is higher in Gm-lpa-TW75-1 as expected at 5 mg/g, followed by GV at 1.6 mg/g whereas Jack, VgD, and Taiwan75 have about 0.3 mg/g. The Gm-lpa-TW75-1 line has the lowest phytate concentration at 1.4 mg/g followed by GV with 1.8 mg/g compared to Taiwan75, VgD, and Jack with 2.5 mg/g. This work describes a high oil and protein soybean line, GV, with increased Pi and lower phytate which will increase the nutritional value for human and animal feed.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T01:27:33Z
2020-12-12T01:27:33Z
2020-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1002/lipd.12253
Lipids.
1558-9307
0024-4201
http://hdl.handle.net/11449/198985
10.1002/lipd.12253
2-s2.0-85086442106
url http://dx.doi.org/10.1002/lipd.12253
http://hdl.handle.net/11449/198985
identifier_str_mv Lipids.
1558-9307
0024-4201
10.1002/lipd.12253
2-s2.0-85086442106
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Lipids
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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