Evaluación de las alteraciones morfológicas y morfométricas de las uniones neuromusculares del diafragma denervado en ratas
Autor(a) principal: | |
---|---|
Data de Publicação: | 2009 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | spa |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4067/S0717-95022009000400043 http://hdl.handle.net/11449/225886 |
Resumo: | The morphological and structural alterations that occur in the neuromuscular junctions of the denervated rat diaphragm were studied. Fifteen adult male albino rats (Rattus norvegicus) aged about 60 days and with a mean weight of 200 g were used. Chronically denervated diaphragms were obtained and the animals were sacrificed after 4, 8 and 12 weeks of denervation. The left antimere of the diaphragm was denervated by section of the phrenic nerve and the right antimere was used as control. Each antimere was divided into three fragments: one was used for histochemical (nonspecific esterase) and morphometric study of neuromuscular junctions, and the other two were used for transmission and scanning electron microscopy (SEM) analysis. Histochemical analysis of the diaphragm neuromuscular junctions after denervation showed only small changes in junction morphology. However, these junctions became smaller and elongated and presented less visible contours with increasing time of denervation. Ultrastructural analysis of neuromuscular junctions after 12 weeks showed more or less organized junctional folds on the muscle fiber surface. The junctional cytoplasm exhibited important alterations such as mitochondrial degeneration and the presence of numerous filaments. SEM revealed the presence of deep primary synaptic grooves with peripheral excavations which housed the nerve terminal boutons and exhibited internally the secondary synaptic clefts present among the junctional folds of the sarcolemma. This study showed that some of the morphological changes demonstrated in other denervated striated skeletal muscles are not repeated at the same intensity or in the same temporal pattern in the rat diaphragm. |
id |
UNSP_644224ed903b3687075cf64cea46428a |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/225886 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Evaluación de las alteraciones morfológicas y morfométricas de las uniones neuromusculares del diafragma denervado en ratasMorphometric and morphological analysis of neuromuscular junction alterations in the denervated rat diaphragmDenervationDiaphragmNeuromuscular junctionRatThe morphological and structural alterations that occur in the neuromuscular junctions of the denervated rat diaphragm were studied. Fifteen adult male albino rats (Rattus norvegicus) aged about 60 days and with a mean weight of 200 g were used. Chronically denervated diaphragms were obtained and the animals were sacrificed after 4, 8 and 12 weeks of denervation. The left antimere of the diaphragm was denervated by section of the phrenic nerve and the right antimere was used as control. Each antimere was divided into three fragments: one was used for histochemical (nonspecific esterase) and morphometric study of neuromuscular junctions, and the other two were used for transmission and scanning electron microscopy (SEM) analysis. Histochemical analysis of the diaphragm neuromuscular junctions after denervation showed only small changes in junction morphology. However, these junctions became smaller and elongated and presented less visible contours with increasing time of denervation. Ultrastructural analysis of neuromuscular junctions after 12 weeks showed more or less organized junctional folds on the muscle fiber surface. The junctional cytoplasm exhibited important alterations such as mitochondrial degeneration and the presence of numerous filaments. SEM revealed the presence of deep primary synaptic grooves with peripheral excavations which housed the nerve terminal boutons and exhibited internally the secondary synaptic clefts present among the junctional folds of the sarcolemma. This study showed that some of the morphological changes demonstrated in other denervated striated skeletal muscles are not repeated at the same intensity or in the same temporal pattern in the rat diaphragm.Medical and Pharmaceutic Sciences Center UNIOESTE Cascavel-ParanaDepartment of Anatomy IB-UNESP, Botucatu-Sao PauloDepartment of Morphophysiological Sciences UEM, Maringa-ParanaDepartment of Physiotherapy UFJF, Juiz de Fora-Minas GeraisDepartment of Anatomy IB-UNESP, Botucatu-Sao PauloCascavel-ParanaUniversidade Estadual Paulista (UNESP)Universidade Estadual de Maringá (UEM)UFJFTorrejais, M. M.Soares, J. C. [UNESP]Matheus, S. M.M. [UNESP]Mello, J. M.Francia-Farje, L. A.D. [UNESP]Vicente, E. J.D.2022-04-28T21:01:26Z2022-04-28T21:01:26Z2009-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1235-1242http://dx.doi.org/10.4067/S0717-95022009000400043International Journal of Morphology, v. 27, n. 4, p. 1235-1242, 2009.0717-95020717-9367http://hdl.handle.net/11449/22588610.4067/S0717-950220090004000432-s2.0-77953571606Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPspaInternational Journal of Morphologyinfo:eu-repo/semantics/openAccess2022-04-28T21:01:26Zoai:repositorio.unesp.br:11449/225886Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:17:19.185312Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Evaluación de las alteraciones morfológicas y morfométricas de las uniones neuromusculares del diafragma denervado en ratas Morphometric and morphological analysis of neuromuscular junction alterations in the denervated rat diaphragm |
title |
Evaluación de las alteraciones morfológicas y morfométricas de las uniones neuromusculares del diafragma denervado en ratas |
spellingShingle |
Evaluación de las alteraciones morfológicas y morfométricas de las uniones neuromusculares del diafragma denervado en ratas Torrejais, M. M. Denervation Diaphragm Neuromuscular junction Rat |
title_short |
Evaluación de las alteraciones morfológicas y morfométricas de las uniones neuromusculares del diafragma denervado en ratas |
title_full |
Evaluación de las alteraciones morfológicas y morfométricas de las uniones neuromusculares del diafragma denervado en ratas |
title_fullStr |
Evaluación de las alteraciones morfológicas y morfométricas de las uniones neuromusculares del diafragma denervado en ratas |
title_full_unstemmed |
Evaluación de las alteraciones morfológicas y morfométricas de las uniones neuromusculares del diafragma denervado en ratas |
title_sort |
Evaluación de las alteraciones morfológicas y morfométricas de las uniones neuromusculares del diafragma denervado en ratas |
author |
Torrejais, M. M. |
author_facet |
Torrejais, M. M. Soares, J. C. [UNESP] Matheus, S. M.M. [UNESP] Mello, J. M. Francia-Farje, L. A.D. [UNESP] Vicente, E. J.D. |
author_role |
author |
author2 |
Soares, J. C. [UNESP] Matheus, S. M.M. [UNESP] Mello, J. M. Francia-Farje, L. A.D. [UNESP] Vicente, E. J.D. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Cascavel-Parana Universidade Estadual Paulista (UNESP) Universidade Estadual de Maringá (UEM) UFJF |
dc.contributor.author.fl_str_mv |
Torrejais, M. M. Soares, J. C. [UNESP] Matheus, S. M.M. [UNESP] Mello, J. M. Francia-Farje, L. A.D. [UNESP] Vicente, E. J.D. |
dc.subject.por.fl_str_mv |
Denervation Diaphragm Neuromuscular junction Rat |
topic |
Denervation Diaphragm Neuromuscular junction Rat |
description |
The morphological and structural alterations that occur in the neuromuscular junctions of the denervated rat diaphragm were studied. Fifteen adult male albino rats (Rattus norvegicus) aged about 60 days and with a mean weight of 200 g were used. Chronically denervated diaphragms were obtained and the animals were sacrificed after 4, 8 and 12 weeks of denervation. The left antimere of the diaphragm was denervated by section of the phrenic nerve and the right antimere was used as control. Each antimere was divided into three fragments: one was used for histochemical (nonspecific esterase) and morphometric study of neuromuscular junctions, and the other two were used for transmission and scanning electron microscopy (SEM) analysis. Histochemical analysis of the diaphragm neuromuscular junctions after denervation showed only small changes in junction morphology. However, these junctions became smaller and elongated and presented less visible contours with increasing time of denervation. Ultrastructural analysis of neuromuscular junctions after 12 weeks showed more or less organized junctional folds on the muscle fiber surface. The junctional cytoplasm exhibited important alterations such as mitochondrial degeneration and the presence of numerous filaments. SEM revealed the presence of deep primary synaptic grooves with peripheral excavations which housed the nerve terminal boutons and exhibited internally the secondary synaptic clefts present among the junctional folds of the sarcolemma. This study showed that some of the morphological changes demonstrated in other denervated striated skeletal muscles are not repeated at the same intensity or in the same temporal pattern in the rat diaphragm. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-01-01 2022-04-28T21:01:26Z 2022-04-28T21:01:26Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4067/S0717-95022009000400043 International Journal of Morphology, v. 27, n. 4, p. 1235-1242, 2009. 0717-9502 0717-9367 http://hdl.handle.net/11449/225886 10.4067/S0717-95022009000400043 2-s2.0-77953571606 |
url |
http://dx.doi.org/10.4067/S0717-95022009000400043 http://hdl.handle.net/11449/225886 |
identifier_str_mv |
International Journal of Morphology, v. 27, n. 4, p. 1235-1242, 2009. 0717-9502 0717-9367 10.4067/S0717-95022009000400043 2-s2.0-77953571606 |
dc.language.iso.fl_str_mv |
spa |
language |
spa |
dc.relation.none.fl_str_mv |
International Journal of Morphology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1235-1242 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128785124425728 |