Effects of ovariectomy on periodontal tissues following tooth replantation
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.archoralbio.2016.01.006 http://hdl.handle.net/11449/172490 |
Resumo: | Objectives The aim of the study was to analyze the effects of ovariectomy on periodontal tissues following immediate tooth replantation by histomorphometric, immunohistochemistry, and μCT analysis. Materials and methods Eighty wistar rats (Rattus norvegicus albinos) with normal estrous cycles were randomly divided into two groups: ovariectomized (OVX) and Sham. Two months after surgery, the rats' upper right incisor was extracted followed by immediate reimplantation. The animals were sacrificed after 28, 45, and 60 days healing time. Histomorphometric and immunohistochemical analysis were performed by evaluation of PCNA and TRAP straining. Results The periodontal ligament was reinserted into the bone and cementum in the both groups. The immunohistochemical analysis revealed PCNA positive cells on the periodontal ligament in both groups at 28 days. Root resorption was noted at 45 days with immunoreactive cells for TRAP present in bone and tooth surface however no statistical differences between the groups were noticed. Histomorphometric analysis showed significant difference between groups in the periodontal ligament and root resorption parameters for the sub-items: intensity of chronic inflammatory infiltrate at 60 days (p < 0.01), the organization of the periodontal ligament at 28 days (p < 0.05), depth of root resorption at 45 days (p < 0.05) and at 60 days (p < 0.001). The μCT analysis showed multiple areas of bone resorption in association with OVX at 28 and 60 days with no significant differences between times in vivo. Conclusion The ovariectomy did not have significant influence in periodontal tissue parameters following tooth reimplantation. |
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Effects of ovariectomy on periodontal tissues following tooth replantationImmunohistochemistryMicro-ctOsteoclastOvariectomyTooth replantationObjectives The aim of the study was to analyze the effects of ovariectomy on periodontal tissues following immediate tooth replantation by histomorphometric, immunohistochemistry, and μCT analysis. Materials and methods Eighty wistar rats (Rattus norvegicus albinos) with normal estrous cycles were randomly divided into two groups: ovariectomized (OVX) and Sham. Two months after surgery, the rats' upper right incisor was extracted followed by immediate reimplantation. The animals were sacrificed after 28, 45, and 60 days healing time. Histomorphometric and immunohistochemical analysis were performed by evaluation of PCNA and TRAP straining. Results The periodontal ligament was reinserted into the bone and cementum in the both groups. The immunohistochemical analysis revealed PCNA positive cells on the periodontal ligament in both groups at 28 days. Root resorption was noted at 45 days with immunoreactive cells for TRAP present in bone and tooth surface however no statistical differences between the groups were noticed. Histomorphometric analysis showed significant difference between groups in the periodontal ligament and root resorption parameters for the sub-items: intensity of chronic inflammatory infiltrate at 60 days (p < 0.01), the organization of the periodontal ligament at 28 days (p < 0.05), depth of root resorption at 45 days (p < 0.05) and at 60 days (p < 0.001). The μCT analysis showed multiple areas of bone resorption in association with OVX at 28 and 60 days with no significant differences between times in vivo. Conclusion The ovariectomy did not have significant influence in periodontal tissue parameters following tooth reimplantation.Department of Surgery and Integrated Clinics Aracatuba Dental School Univ Estadual Paulista UNESP, 1193 - Vila Mendonca, Rua Jose BonifacioCenter for Craniofacial Regeneration College of Dental Medicine Columbia University, 630 West 168th St.Department of Basic Science Aracatuba Dental School Univ Estadual Paulista UNESP, KM 527/528 Campus Universitario, Rodovia Marechal RondonBiosciences Institute of Botucatu UNESP Univ Estadual PaulistaDepartment of Biomaterials and Biomimetics New York University College of Dentistry, 433 1st Ave.Department of Periodontology and Implant Dentistry New York University College of Dentistry, 345 E 24th StDivision of Engineering New York University Abu DhabiDepartment of Surgery and Integrated Clinics Aracatuba Dental School Univ Estadual Paulista UNESP, 1193 - Vila Mendonca, Rua Jose BonifacioDepartment of Basic Science Aracatuba Dental School Univ Estadual Paulista UNESP, KM 527/528 Campus Universitario, Rodovia Marechal RondonBiosciences Institute of Botucatu UNESP Univ Estadual PaulistaUniversidade Estadual Paulista (Unesp)Columbia UniversityNew York University College of DentistryNew York University Abu DhabiMarão, Heloisa Fonseca [UNESP]Mao, Jeremy J.Casatti, Cláudio Aparecido [UNESP]Coelho, Paulo G.Ervolino, Edilson [UNESP]Zhou, JianSilva, Vanessa Ferreira Da [UNESP]Panzarini, Sônia Regina [UNESP]2018-12-11T17:00:37Z2018-12-11T17:00:37Z2016-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9-16application/pdfhttp://dx.doi.org/10.1016/j.archoralbio.2016.01.006Archives of Oral Biology, v. 65, p. 9-16.1879-15060003-9969http://hdl.handle.net/11449/17249010.1016/j.archoralbio.2016.01.0062-s2.0-849567518432-s2.0-84956751843.pdf440809551734684684874626269318770000-0003-4859-05830000-0001-5650-7343Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Oral Biology0,752info:eu-repo/semantics/openAccess2023-11-25T06:14:04Zoai:repositorio.unesp.br:11449/172490Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:40:02.037604Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effects of ovariectomy on periodontal tissues following tooth replantation |
title |
Effects of ovariectomy on periodontal tissues following tooth replantation |
spellingShingle |
Effects of ovariectomy on periodontal tissues following tooth replantation Marão, Heloisa Fonseca [UNESP] Immunohistochemistry Micro-ct Osteoclast Ovariectomy Tooth replantation |
title_short |
Effects of ovariectomy on periodontal tissues following tooth replantation |
title_full |
Effects of ovariectomy on periodontal tissues following tooth replantation |
title_fullStr |
Effects of ovariectomy on periodontal tissues following tooth replantation |
title_full_unstemmed |
Effects of ovariectomy on periodontal tissues following tooth replantation |
title_sort |
Effects of ovariectomy on periodontal tissues following tooth replantation |
author |
Marão, Heloisa Fonseca [UNESP] |
author_facet |
Marão, Heloisa Fonseca [UNESP] Mao, Jeremy J. Casatti, Cláudio Aparecido [UNESP] Coelho, Paulo G. Ervolino, Edilson [UNESP] Zhou, Jian Silva, Vanessa Ferreira Da [UNESP] Panzarini, Sônia Regina [UNESP] |
author_role |
author |
author2 |
Mao, Jeremy J. Casatti, Cláudio Aparecido [UNESP] Coelho, Paulo G. Ervolino, Edilson [UNESP] Zhou, Jian Silva, Vanessa Ferreira Da [UNESP] Panzarini, Sônia Regina [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Columbia University New York University College of Dentistry New York University Abu Dhabi |
dc.contributor.author.fl_str_mv |
Marão, Heloisa Fonseca [UNESP] Mao, Jeremy J. Casatti, Cláudio Aparecido [UNESP] Coelho, Paulo G. Ervolino, Edilson [UNESP] Zhou, Jian Silva, Vanessa Ferreira Da [UNESP] Panzarini, Sônia Regina [UNESP] |
dc.subject.por.fl_str_mv |
Immunohistochemistry Micro-ct Osteoclast Ovariectomy Tooth replantation |
topic |
Immunohistochemistry Micro-ct Osteoclast Ovariectomy Tooth replantation |
description |
Objectives The aim of the study was to analyze the effects of ovariectomy on periodontal tissues following immediate tooth replantation by histomorphometric, immunohistochemistry, and μCT analysis. Materials and methods Eighty wistar rats (Rattus norvegicus albinos) with normal estrous cycles were randomly divided into two groups: ovariectomized (OVX) and Sham. Two months after surgery, the rats' upper right incisor was extracted followed by immediate reimplantation. The animals were sacrificed after 28, 45, and 60 days healing time. Histomorphometric and immunohistochemical analysis were performed by evaluation of PCNA and TRAP straining. Results The periodontal ligament was reinserted into the bone and cementum in the both groups. The immunohistochemical analysis revealed PCNA positive cells on the periodontal ligament in both groups at 28 days. Root resorption was noted at 45 days with immunoreactive cells for TRAP present in bone and tooth surface however no statistical differences between the groups were noticed. Histomorphometric analysis showed significant difference between groups in the periodontal ligament and root resorption parameters for the sub-items: intensity of chronic inflammatory infiltrate at 60 days (p < 0.01), the organization of the periodontal ligament at 28 days (p < 0.05), depth of root resorption at 45 days (p < 0.05) and at 60 days (p < 0.001). The μCT analysis showed multiple areas of bone resorption in association with OVX at 28 and 60 days with no significant differences between times in vivo. Conclusion The ovariectomy did not have significant influence in periodontal tissue parameters following tooth reimplantation. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-05-01 2018-12-11T17:00:37Z 2018-12-11T17:00:37Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.archoralbio.2016.01.006 Archives of Oral Biology, v. 65, p. 9-16. 1879-1506 0003-9969 http://hdl.handle.net/11449/172490 10.1016/j.archoralbio.2016.01.006 2-s2.0-84956751843 2-s2.0-84956751843.pdf 4408095517346846 8487462626931877 0000-0003-4859-0583 0000-0001-5650-7343 |
url |
http://dx.doi.org/10.1016/j.archoralbio.2016.01.006 http://hdl.handle.net/11449/172490 |
identifier_str_mv |
Archives of Oral Biology, v. 65, p. 9-16. 1879-1506 0003-9969 10.1016/j.archoralbio.2016.01.006 2-s2.0-84956751843 2-s2.0-84956751843.pdf 4408095517346846 8487462626931877 0000-0003-4859-0583 0000-0001-5650-7343 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Archives of Oral Biology 0,752 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
9-16 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128963773464576 |