Free radical scavenging activity of Kielmeyera variabilis (Clusiaceae)
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/molecules18022376 http://hdl.handle.net/11449/74545 |
Resumo: | As part of our ongoing research on antioxidant agents from Brazilian flora, we screened the free radical scavenging activity of two extracts and eight fractions of Kielmeyera variabilis (Clusiaceae) using DPPH (2,2-diphenyl-1- picrylhydrazyl-hydrate) and ABTS+ [2,2'-azinobis(3- ethylenebenzothiazoline-6-sulfonic acid)] colorimetric assays. The ethyl acetate and n-butanol fractions of the leaves of K. variabilis displayed the strongest activity (IC50 of 3.5 ± 0.3 and 4.4 ± 0.2 μg mL -1 for DPPH and 6.6 ± 0.4 and 3.1 ± 0.1 μg mL -1 for ABTS+, respectively). Chromatographic fractionation of the most potent fractions led to identification of three flavonols with previously described antioxidant activity, quercitrin (1), quercetin-3-O- glucoside (3), and quercetin-3-O-galactoside (4), and of one biflavone, podocarpusflavone A (2). This is the first time that the presence of these flavonoids in Kielmeyera variabilis has been reported. © 2013 by the authors. |
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Free radical scavenging activity of Kielmeyera variabilis (Clusiaceae)ABTS+AntioxidantClusiaceaeDPPHFlavonoidFlavonolFree radical scavenging activityKielmeyera variabilis1,1 diphenyl 2 picrylhydrazyl2,2' azino di (3 ethylbenzothiazoline) 6 sulfonic acid2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid2,2-diphenyl-1-picrylhydrazylbenzothiazole derivativebiphenyl derivativeflavonoidpicric acidscavengersulfonic acid derivativechemistryIC 50metabolismBenzothiazolesBiphenyl CompoundsFlavonoidsFree Radical ScavengersInhibitory Concentration 50PicratesSulfonic AcidsAs part of our ongoing research on antioxidant agents from Brazilian flora, we screened the free radical scavenging activity of two extracts and eight fractions of Kielmeyera variabilis (Clusiaceae) using DPPH (2,2-diphenyl-1- picrylhydrazyl-hydrate) and ABTS+ [2,2'-azinobis(3- ethylenebenzothiazoline-6-sulfonic acid)] colorimetric assays. The ethyl acetate and n-butanol fractions of the leaves of K. variabilis displayed the strongest activity (IC50 of 3.5 ± 0.3 and 4.4 ± 0.2 μg mL -1 for DPPH and 6.6 ± 0.4 and 3.1 ± 0.1 μg mL -1 for ABTS+, respectively). Chromatographic fractionation of the most potent fractions led to identification of three flavonols with previously described antioxidant activity, quercitrin (1), quercetin-3-O- glucoside (3), and quercetin-3-O-galactoside (4), and of one biflavone, podocarpusflavone A (2). This is the first time that the presence of these flavonoids in Kielmeyera variabilis has been reported. © 2013 by the authors.Department of Organic Chemistry Institute of Chemistry São Paulo State University, Araraquara 14800-900Department of Organic Chemistry Institute of Chemistry São Paulo State University, Araraquara 14800-900Universidade Estadual Paulista (Unesp)Coqueiro, Aline [UNESP]Regasini, Luis Octávio [UNESP]Skrzek, Scheila Cristina Gutkoski [UNESP]Queiroz, Marcos Marçal Ferreira [UNESP]Silva, Dulce Helena Siqueira [UNESP]Bolzani, Vanderlan da Silva [UNESP]2014-05-27T11:28:21Z2014-05-27T11:28:21Z2013-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2376-2385application/pdfhttp://dx.doi.org/10.3390/molecules18022376Molecules, v. 18, n. 2, p. 2376-2385, 2013.1420-3049http://hdl.handle.net/11449/7454510.3390/molecules18022376WOS:0003154006000762-s2.0-848746253752-s2.0-84874625375.pdf4702004904231248448408368525167309927364527645500000-0002-1516-7765Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMolecules3.0980,855info:eu-repo/semantics/openAccess2023-10-30T06:10:08Zoai:repositorio.unesp.br:11449/74545Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:27:13.631533Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Free radical scavenging activity of Kielmeyera variabilis (Clusiaceae) |
title |
Free radical scavenging activity of Kielmeyera variabilis (Clusiaceae) |
spellingShingle |
Free radical scavenging activity of Kielmeyera variabilis (Clusiaceae) Coqueiro, Aline [UNESP] ABTS+ Antioxidant Clusiaceae DPPH Flavonoid Flavonol Free radical scavenging activity Kielmeyera variabilis 1,1 diphenyl 2 picrylhydrazyl 2,2' azino di (3 ethylbenzothiazoline) 6 sulfonic acid 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid 2,2-diphenyl-1-picrylhydrazyl benzothiazole derivative biphenyl derivative flavonoid picric acid scavenger sulfonic acid derivative chemistry IC 50 metabolism Benzothiazoles Biphenyl Compounds Flavonoids Free Radical Scavengers Inhibitory Concentration 50 Picrates Sulfonic Acids |
title_short |
Free radical scavenging activity of Kielmeyera variabilis (Clusiaceae) |
title_full |
Free radical scavenging activity of Kielmeyera variabilis (Clusiaceae) |
title_fullStr |
Free radical scavenging activity of Kielmeyera variabilis (Clusiaceae) |
title_full_unstemmed |
Free radical scavenging activity of Kielmeyera variabilis (Clusiaceae) |
title_sort |
Free radical scavenging activity of Kielmeyera variabilis (Clusiaceae) |
author |
Coqueiro, Aline [UNESP] |
author_facet |
Coqueiro, Aline [UNESP] Regasini, Luis Octávio [UNESP] Skrzek, Scheila Cristina Gutkoski [UNESP] Queiroz, Marcos Marçal Ferreira [UNESP] Silva, Dulce Helena Siqueira [UNESP] Bolzani, Vanderlan da Silva [UNESP] |
author_role |
author |
author2 |
Regasini, Luis Octávio [UNESP] Skrzek, Scheila Cristina Gutkoski [UNESP] Queiroz, Marcos Marçal Ferreira [UNESP] Silva, Dulce Helena Siqueira [UNESP] Bolzani, Vanderlan da Silva [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Coqueiro, Aline [UNESP] Regasini, Luis Octávio [UNESP] Skrzek, Scheila Cristina Gutkoski [UNESP] Queiroz, Marcos Marçal Ferreira [UNESP] Silva, Dulce Helena Siqueira [UNESP] Bolzani, Vanderlan da Silva [UNESP] |
dc.subject.por.fl_str_mv |
ABTS+ Antioxidant Clusiaceae DPPH Flavonoid Flavonol Free radical scavenging activity Kielmeyera variabilis 1,1 diphenyl 2 picrylhydrazyl 2,2' azino di (3 ethylbenzothiazoline) 6 sulfonic acid 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid 2,2-diphenyl-1-picrylhydrazyl benzothiazole derivative biphenyl derivative flavonoid picric acid scavenger sulfonic acid derivative chemistry IC 50 metabolism Benzothiazoles Biphenyl Compounds Flavonoids Free Radical Scavengers Inhibitory Concentration 50 Picrates Sulfonic Acids |
topic |
ABTS+ Antioxidant Clusiaceae DPPH Flavonoid Flavonol Free radical scavenging activity Kielmeyera variabilis 1,1 diphenyl 2 picrylhydrazyl 2,2' azino di (3 ethylbenzothiazoline) 6 sulfonic acid 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid 2,2-diphenyl-1-picrylhydrazyl benzothiazole derivative biphenyl derivative flavonoid picric acid scavenger sulfonic acid derivative chemistry IC 50 metabolism Benzothiazoles Biphenyl Compounds Flavonoids Free Radical Scavengers Inhibitory Concentration 50 Picrates Sulfonic Acids |
description |
As part of our ongoing research on antioxidant agents from Brazilian flora, we screened the free radical scavenging activity of two extracts and eight fractions of Kielmeyera variabilis (Clusiaceae) using DPPH (2,2-diphenyl-1- picrylhydrazyl-hydrate) and ABTS+ [2,2'-azinobis(3- ethylenebenzothiazoline-6-sulfonic acid)] colorimetric assays. The ethyl acetate and n-butanol fractions of the leaves of K. variabilis displayed the strongest activity (IC50 of 3.5 ± 0.3 and 4.4 ± 0.2 μg mL -1 for DPPH and 6.6 ± 0.4 and 3.1 ± 0.1 μg mL -1 for ABTS+, respectively). Chromatographic fractionation of the most potent fractions led to identification of three flavonols with previously described antioxidant activity, quercitrin (1), quercetin-3-O- glucoside (3), and quercetin-3-O-galactoside (4), and of one biflavone, podocarpusflavone A (2). This is the first time that the presence of these flavonoids in Kielmeyera variabilis has been reported. © 2013 by the authors. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-02-01 2014-05-27T11:28:21Z 2014-05-27T11:28:21Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/molecules18022376 Molecules, v. 18, n. 2, p. 2376-2385, 2013. 1420-3049 http://hdl.handle.net/11449/74545 10.3390/molecules18022376 WOS:000315400600076 2-s2.0-84874625375 2-s2.0-84874625375.pdf 4702004904231248 4484083685251673 0992736452764550 0000-0002-1516-7765 |
url |
http://dx.doi.org/10.3390/molecules18022376 http://hdl.handle.net/11449/74545 |
identifier_str_mv |
Molecules, v. 18, n. 2, p. 2376-2385, 2013. 1420-3049 10.3390/molecules18022376 WOS:000315400600076 2-s2.0-84874625375 2-s2.0-84874625375.pdf 4702004904231248 4484083685251673 0992736452764550 0000-0002-1516-7765 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Molecules 3.098 0,855 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
2376-2385 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128655282405376 |