Effects of paternal diet and antioxidant addition to the semen extender on bovine semen characteristics and on the phenotype of the resulting embryo
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.theriogenology.2021.08.031 http://hdl.handle.net/11449/229433 |
Resumo: | The aim of this study was to examine the effects of long-term dietary supplementation of young Nellore bulls with rumen-protected polyunsaturated fatty acids (PUFAs) and of the inclusion of catalase in the semen extender on semen quality, in vitro sperm fertilizing ability, and intracytoplasmic lipid content in the resulting embryos. Twelve Nellore bulls were supplemented with rumen-protected PUFAs or with a basal diet from 14 to 24 months of age. The semen was collected at the end of supplementation. For cryopreservation, the ejaculate was divided into two equal volumes and catalase was added to the extender in one of the fractions. Thus, the experimental design consisted of a 2 × 2 factorial scheme with two diets (control and PUFA) and two extenders (Cat+ and Cat-). Total motility and the percentage of rapid cells in fresh semen were negatively affected by dietary supplementation with PUFAs (P < 0.05), but these effects did not persist after freezing. The frozen/thawed semen of animals fed PUFAs exhibited an increase in the percentages of damaged plasma and acrosomal membranes, as well as an increase in the proportion of lipids ions at m/z 578 and m/z 757 detected by MALDI-MS. Nevertheless, there was no effect of the treatments on in vitro embryo development. However, embryos derived from bulls supplemented with PUFAs exhibited higher lipid accumulation compared to control (P < 0.05). In conclusion, PUFA supplementation promoted worsening of semen quality without affecting the in vitro sperm fertilizing ability; however, the paternal diet affected the intracytoplasmic lipid content in the resulting embryos. |
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Repositório Institucional da UNESP |
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2946 |
spelling |
Effects of paternal diet and antioxidant addition to the semen extender on bovine semen characteristics and on the phenotype of the resulting embryoBullLipidNongenetic inheritanceNutritionPaternal effectPolyunsaturated fatty acidsThe aim of this study was to examine the effects of long-term dietary supplementation of young Nellore bulls with rumen-protected polyunsaturated fatty acids (PUFAs) and of the inclusion of catalase in the semen extender on semen quality, in vitro sperm fertilizing ability, and intracytoplasmic lipid content in the resulting embryos. Twelve Nellore bulls were supplemented with rumen-protected PUFAs or with a basal diet from 14 to 24 months of age. The semen was collected at the end of supplementation. For cryopreservation, the ejaculate was divided into two equal volumes and catalase was added to the extender in one of the fractions. Thus, the experimental design consisted of a 2 × 2 factorial scheme with two diets (control and PUFA) and two extenders (Cat+ and Cat-). Total motility and the percentage of rapid cells in fresh semen were negatively affected by dietary supplementation with PUFAs (P < 0.05), but these effects did not persist after freezing. The frozen/thawed semen of animals fed PUFAs exhibited an increase in the percentages of damaged plasma and acrosomal membranes, as well as an increase in the proportion of lipids ions at m/z 578 and m/z 757 detected by MALDI-MS. Nevertheless, there was no effect of the treatments on in vitro embryo development. However, embryos derived from bulls supplemented with PUFAs exhibited higher lipid accumulation compared to control (P < 0.05). In conclusion, PUFA supplementation promoted worsening of semen quality without affecting the in vitro sperm fertilizing ability; however, the paternal diet affected the intracytoplasmic lipid content in the resulting embryos.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)São Paulo State University (UNESP) Department of Animal Reproduction Graduate Program in Genetics and Animal Breeding School of Agrarian and Veterinary Sciences Campus JaboticabalSão Paulo State University (UNESP) Department of Animal Reproduction Graduate Program in Veterinary Medicine School of Agrarian and Veterinary Sciences Campus JaboticabalProteomics Center of Excellence Northwestern University, 2170 Campus DrThoMSon Mass Spectrometry Laboratory Institute of Chemistry University of Campinas Cidade Universitaária Zeferino Vaz, CampinasDepartment of Chemistry Purdue UniversityInstituto de Zootecnia (IZ/APTA), SertãozinhoSão Paulo State University (UNESP) School of Veterinary Medicine and Animal Science, Campus BotucatuSão Paulo State University (UNESP) School of Veterinary Medicine Laboratory of Reproductive Physiology, Campus AraçatubaSão Paulo State University (UNESP) Department of Animal Reproduction Graduate Program in Genetics and Animal Breeding School of Agrarian and Veterinary Sciences Campus JaboticabalSão Paulo State University (UNESP) Department of Animal Reproduction Graduate Program in Veterinary Medicine School of Agrarian and Veterinary Sciences Campus JaboticabalSão Paulo State University (UNESP) School of Veterinary Medicine and Animal Science, Campus BotucatuSão Paulo State University (UNESP) School of Veterinary Medicine Laboratory of Reproductive Physiology, Campus AraçatubaFAPESP: #2013/13696–3FAPESP: #2015/06733–5FAPESP: #2019/11174–6CNPq: #307416/2015–1CNPq: #314136/2018–5CAPES: 001Universidade Estadual Paulista (UNESP)Northwestern UniversityUniversidade Estadual de Campinas (UNICAMP)Purdue UniversityInstituto de Zootecnia (IZ/APTA)Bastos, Natália Marins [UNESP]Rossi, Guilherme Fazan [UNESP]da Silva Leão, Beatriz Caetano [UNESP]Negrão, FernandaFerreira, Christina RamiresVrisman, Dayane Priscila [UNESP]Rodrigues, Naiara Nantes [UNESP]Zorzetto, Mariana Furtadode Paula Freitas-Dell'Aqua, Camila [UNESP]Vantini, Roberta [UNESP]Monteiro, Fabio Morato [UNESP]Mercadante, Maria Eugênia ZerlottiEberlin, Marcos Nogueirade Lima, Vera Fernanda Martins Hossepian [UNESP]Mingoti, Gisele Zoccal [UNESP]2022-04-29T08:32:33Z2022-04-29T08:32:33Z2021-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article23-33http://dx.doi.org/10.1016/j.theriogenology.2021.08.031Theriogenology, v. 175, p. 23-33.0093-691Xhttp://hdl.handle.net/11449/22943310.1016/j.theriogenology.2021.08.0312-s2.0-85113912213Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTheriogenologyinfo:eu-repo/semantics/openAccess2022-04-29T08:32:33Zoai:repositorio.unesp.br:11449/229433Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-29T08:32:33Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effects of paternal diet and antioxidant addition to the semen extender on bovine semen characteristics and on the phenotype of the resulting embryo |
title |
Effects of paternal diet and antioxidant addition to the semen extender on bovine semen characteristics and on the phenotype of the resulting embryo |
spellingShingle |
Effects of paternal diet and antioxidant addition to the semen extender on bovine semen characteristics and on the phenotype of the resulting embryo Bastos, Natália Marins [UNESP] Bull Lipid Nongenetic inheritance Nutrition Paternal effect Polyunsaturated fatty acids |
title_short |
Effects of paternal diet and antioxidant addition to the semen extender on bovine semen characteristics and on the phenotype of the resulting embryo |
title_full |
Effects of paternal diet and antioxidant addition to the semen extender on bovine semen characteristics and on the phenotype of the resulting embryo |
title_fullStr |
Effects of paternal diet and antioxidant addition to the semen extender on bovine semen characteristics and on the phenotype of the resulting embryo |
title_full_unstemmed |
Effects of paternal diet and antioxidant addition to the semen extender on bovine semen characteristics and on the phenotype of the resulting embryo |
title_sort |
Effects of paternal diet and antioxidant addition to the semen extender on bovine semen characteristics and on the phenotype of the resulting embryo |
author |
Bastos, Natália Marins [UNESP] |
author_facet |
Bastos, Natália Marins [UNESP] Rossi, Guilherme Fazan [UNESP] da Silva Leão, Beatriz Caetano [UNESP] Negrão, Fernanda Ferreira, Christina Ramires Vrisman, Dayane Priscila [UNESP] Rodrigues, Naiara Nantes [UNESP] Zorzetto, Mariana Furtado de Paula Freitas-Dell'Aqua, Camila [UNESP] Vantini, Roberta [UNESP] Monteiro, Fabio Morato [UNESP] Mercadante, Maria Eugênia Zerlotti Eberlin, Marcos Nogueira de Lima, Vera Fernanda Martins Hossepian [UNESP] Mingoti, Gisele Zoccal [UNESP] |
author_role |
author |
author2 |
Rossi, Guilherme Fazan [UNESP] da Silva Leão, Beatriz Caetano [UNESP] Negrão, Fernanda Ferreira, Christina Ramires Vrisman, Dayane Priscila [UNESP] Rodrigues, Naiara Nantes [UNESP] Zorzetto, Mariana Furtado de Paula Freitas-Dell'Aqua, Camila [UNESP] Vantini, Roberta [UNESP] Monteiro, Fabio Morato [UNESP] Mercadante, Maria Eugênia Zerlotti Eberlin, Marcos Nogueira de Lima, Vera Fernanda Martins Hossepian [UNESP] Mingoti, Gisele Zoccal [UNESP] |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Northwestern University Universidade Estadual de Campinas (UNICAMP) Purdue University Instituto de Zootecnia (IZ/APTA) |
dc.contributor.author.fl_str_mv |
Bastos, Natália Marins [UNESP] Rossi, Guilherme Fazan [UNESP] da Silva Leão, Beatriz Caetano [UNESP] Negrão, Fernanda Ferreira, Christina Ramires Vrisman, Dayane Priscila [UNESP] Rodrigues, Naiara Nantes [UNESP] Zorzetto, Mariana Furtado de Paula Freitas-Dell'Aqua, Camila [UNESP] Vantini, Roberta [UNESP] Monteiro, Fabio Morato [UNESP] Mercadante, Maria Eugênia Zerlotti Eberlin, Marcos Nogueira de Lima, Vera Fernanda Martins Hossepian [UNESP] Mingoti, Gisele Zoccal [UNESP] |
dc.subject.por.fl_str_mv |
Bull Lipid Nongenetic inheritance Nutrition Paternal effect Polyunsaturated fatty acids |
topic |
Bull Lipid Nongenetic inheritance Nutrition Paternal effect Polyunsaturated fatty acids |
description |
The aim of this study was to examine the effects of long-term dietary supplementation of young Nellore bulls with rumen-protected polyunsaturated fatty acids (PUFAs) and of the inclusion of catalase in the semen extender on semen quality, in vitro sperm fertilizing ability, and intracytoplasmic lipid content in the resulting embryos. Twelve Nellore bulls were supplemented with rumen-protected PUFAs or with a basal diet from 14 to 24 months of age. The semen was collected at the end of supplementation. For cryopreservation, the ejaculate was divided into two equal volumes and catalase was added to the extender in one of the fractions. Thus, the experimental design consisted of a 2 × 2 factorial scheme with two diets (control and PUFA) and two extenders (Cat+ and Cat-). Total motility and the percentage of rapid cells in fresh semen were negatively affected by dietary supplementation with PUFAs (P < 0.05), but these effects did not persist after freezing. The frozen/thawed semen of animals fed PUFAs exhibited an increase in the percentages of damaged plasma and acrosomal membranes, as well as an increase in the proportion of lipids ions at m/z 578 and m/z 757 detected by MALDI-MS. Nevertheless, there was no effect of the treatments on in vitro embryo development. However, embryos derived from bulls supplemented with PUFAs exhibited higher lipid accumulation compared to control (P < 0.05). In conclusion, PUFA supplementation promoted worsening of semen quality without affecting the in vitro sperm fertilizing ability; however, the paternal diet affected the intracytoplasmic lipid content in the resulting embryos. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-11-01 2022-04-29T08:32:33Z 2022-04-29T08:32:33Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.theriogenology.2021.08.031 Theriogenology, v. 175, p. 23-33. 0093-691X http://hdl.handle.net/11449/229433 10.1016/j.theriogenology.2021.08.031 2-s2.0-85113912213 |
url |
http://dx.doi.org/10.1016/j.theriogenology.2021.08.031 http://hdl.handle.net/11449/229433 |
identifier_str_mv |
Theriogenology, v. 175, p. 23-33. 0093-691X 10.1016/j.theriogenology.2021.08.031 2-s2.0-85113912213 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Theriogenology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
23-33 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965750432104448 |