Evaluation of graphene oxide and reduced graphene oxide in the immobilization of laccase enzyme and its application in the determination of dopamine

Detalhes bibliográficos
Autor(a) principal: Kohori, Natália Akemi [UNESP]
Data de Publicação: 2018
Outros Autores: da Silva, Martin Kássio Leme [UNESP], Cesarino, Ivana [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s10008-017-3738-5
http://hdl.handle.net/11449/170063
Resumo: Bioelectrodes were developed based on a simple deposition of graphene oxide (GO) or reduced graphed oxide (rGO) and laccase (Lac) on a glassy carbon (GC) electrode surface. The morphology and electrochemical behavior of the biosensors were characterized by scanning electron microscopy and cyclic voltammetry. These results demonstrated that only rGO was successfully applied for the immobilization of the laccase enzyme, improving the analytical signal for the determination of dopamine. The GC/rGO/Lac biosensor was applied to the detection of dopamine in synthetic urine and plasmatic serum samples, achieving a detection limit of 91.0 nmol L−1.
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spelling Evaluation of graphene oxide and reduced graphene oxide in the immobilization of laccase enzyme and its application in the determination of dopamineBiosensorsDopamineGrapheneLaccaseBioelectrodes were developed based on a simple deposition of graphene oxide (GO) or reduced graphed oxide (rGO) and laccase (Lac) on a glassy carbon (GC) electrode surface. The morphology and electrochemical behavior of the biosensors were characterized by scanning electron microscopy and cyclic voltammetry. These results demonstrated that only rGO was successfully applied for the immobilization of the laccase enzyme, improving the analytical signal for the determination of dopamine. The GC/rGO/Lac biosensor was applied to the detection of dopamine in synthetic urine and plasmatic serum samples, achieving a detection limit of 91.0 nmol L−1.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)School of Agriculture Sao Paulo State University (UNESP)School of Agriculture Sao Paulo State University (UNESP)FAPESP: 2015/02136-2Universidade Estadual Paulista (Unesp)Kohori, Natália Akemi [UNESP]da Silva, Martin Kássio Leme [UNESP]Cesarino, Ivana [UNESP]2018-12-11T16:48:58Z2018-12-11T16:48:58Z2018-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article141-148application/pdfhttp://dx.doi.org/10.1007/s10008-017-3738-5Journal of Solid State Electrochemistry, v. 22, n. 1, p. 141-148, 2018.1432-8488http://hdl.handle.net/11449/17006310.1007/s10008-017-3738-52-s2.0-850283330772-s2.0-85028333077.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Solid State Electrochemistry0,661info:eu-repo/semantics/openAccess2024-01-02T06:21:47Zoai:repositorio.unesp.br:11449/170063Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:56:44.177722Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Evaluation of graphene oxide and reduced graphene oxide in the immobilization of laccase enzyme and its application in the determination of dopamine
title Evaluation of graphene oxide and reduced graphene oxide in the immobilization of laccase enzyme and its application in the determination of dopamine
spellingShingle Evaluation of graphene oxide and reduced graphene oxide in the immobilization of laccase enzyme and its application in the determination of dopamine
Kohori, Natália Akemi [UNESP]
Biosensors
Dopamine
Graphene
Laccase
title_short Evaluation of graphene oxide and reduced graphene oxide in the immobilization of laccase enzyme and its application in the determination of dopamine
title_full Evaluation of graphene oxide and reduced graphene oxide in the immobilization of laccase enzyme and its application in the determination of dopamine
title_fullStr Evaluation of graphene oxide and reduced graphene oxide in the immobilization of laccase enzyme and its application in the determination of dopamine
title_full_unstemmed Evaluation of graphene oxide and reduced graphene oxide in the immobilization of laccase enzyme and its application in the determination of dopamine
title_sort Evaluation of graphene oxide and reduced graphene oxide in the immobilization of laccase enzyme and its application in the determination of dopamine
author Kohori, Natália Akemi [UNESP]
author_facet Kohori, Natália Akemi [UNESP]
da Silva, Martin Kássio Leme [UNESP]
Cesarino, Ivana [UNESP]
author_role author
author2 da Silva, Martin Kássio Leme [UNESP]
Cesarino, Ivana [UNESP]
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Kohori, Natália Akemi [UNESP]
da Silva, Martin Kássio Leme [UNESP]
Cesarino, Ivana [UNESP]
dc.subject.por.fl_str_mv Biosensors
Dopamine
Graphene
Laccase
topic Biosensors
Dopamine
Graphene
Laccase
description Bioelectrodes were developed based on a simple deposition of graphene oxide (GO) or reduced graphed oxide (rGO) and laccase (Lac) on a glassy carbon (GC) electrode surface. The morphology and electrochemical behavior of the biosensors were characterized by scanning electron microscopy and cyclic voltammetry. These results demonstrated that only rGO was successfully applied for the immobilization of the laccase enzyme, improving the analytical signal for the determination of dopamine. The GC/rGO/Lac biosensor was applied to the detection of dopamine in synthetic urine and plasmatic serum samples, achieving a detection limit of 91.0 nmol L−1.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-11T16:48:58Z
2018-12-11T16:48:58Z
2018-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s10008-017-3738-5
Journal of Solid State Electrochemistry, v. 22, n. 1, p. 141-148, 2018.
1432-8488
http://hdl.handle.net/11449/170063
10.1007/s10008-017-3738-5
2-s2.0-85028333077
2-s2.0-85028333077.pdf
url http://dx.doi.org/10.1007/s10008-017-3738-5
http://hdl.handle.net/11449/170063
identifier_str_mv Journal of Solid State Electrochemistry, v. 22, n. 1, p. 141-148, 2018.
1432-8488
10.1007/s10008-017-3738-5
2-s2.0-85028333077
2-s2.0-85028333077.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Solid State Electrochemistry
0,661
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 141-148
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129376662847488

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