Systemic Administration of Proteoglycan Protects BALB/c Retired Breeder Mice from Experimental Arthritis
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1155/2016/6765134 http://hdl.handle.net/11449/173034 |
Resumo: | This study was undertaken to evaluate the prophylactic potential of proteoglycan (PG) administration in experimental arthritis. Female BALB/c retired breeder mice received two (2xPG50 and 2xPG100 groups) or three (3xPG50 group) intraperitoneal doses of bovine PG (50 μg or 100 μg) every three days. A week later the animals were submitted to arthritis induction by immunization with three i.p. doses of bovine PG associated with dimethyldioctadecylammonium bromide adjuvant at intervals of 21 days. Disease severity was daily assessed after the third dose by score evaluation. The 3xPG50 group showed significant reduction in prevalence and clinical scores. This protective effect was associated with lower production of IFN-γ and IL-17 and increased production of IL-5 and IL-10 by spleen cells restimulated in vitro with PG. Even though previous PG administration restrained dendritic cells maturation this procedure did not alter the frequency of regulatory Foxp3+ T cells. Lower TNF-α and IL-6 levels and higher expression of ROR-γ and GATA-3 were detected in the paws of protected animals. A delayed-type hypersensitivity reaction confirmed specific tolerance induction. Taken together, these results indicate that previous PG inoculation determines a specific tolerogenic effect that is able to decrease severity of subsequently induced arthritis. |
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Repositório Institucional da UNESP |
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Systemic Administration of Proteoglycan Protects BALB/c Retired Breeder Mice from Experimental ArthritisThis study was undertaken to evaluate the prophylactic potential of proteoglycan (PG) administration in experimental arthritis. Female BALB/c retired breeder mice received two (2xPG50 and 2xPG100 groups) or three (3xPG50 group) intraperitoneal doses of bovine PG (50 μg or 100 μg) every three days. A week later the animals were submitted to arthritis induction by immunization with three i.p. doses of bovine PG associated with dimethyldioctadecylammonium bromide adjuvant at intervals of 21 days. Disease severity was daily assessed after the third dose by score evaluation. The 3xPG50 group showed significant reduction in prevalence and clinical scores. This protective effect was associated with lower production of IFN-γ and IL-17 and increased production of IL-5 and IL-10 by spleen cells restimulated in vitro with PG. Even though previous PG administration restrained dendritic cells maturation this procedure did not alter the frequency of regulatory Foxp3+ T cells. Lower TNF-α and IL-6 levels and higher expression of ROR-γ and GATA-3 were detected in the paws of protected animals. A delayed-type hypersensitivity reaction confirmed specific tolerance induction. Taken together, these results indicate that previous PG inoculation determines a specific tolerogenic effect that is able to decrease severity of subsequently induced arthritis.Department of Microbiology and Immunology Institute of Biosciences of Botucatu S�o Paulo State University (UNESP)Department of Pathology Botucatu Medical SchoolLaboratory of Flow Cytometry Amaral Carvalho FoundationDepartment of Microbiology and Immunology Institute of Biosciences of Botucatu S�o Paulo State University (UNESP)Department of Pathology Botucatu Medical SchoolUniversidade Estadual Paulista (Unesp)Amaral Carvalho FoundationIshikawa, Larissa Lumi Watanabe [UNESP]Colavite, Priscila Maria [UNESP]Fraga-Silva, Thais Fernanda De Campos [UNESP]Mimura, Luiza Ayumi Nishiyama [UNESP]Fran�a, Thais Graziela Doneg� [UNESP]Zorzella-Pezavento, Sofia Fernanda Gon�alves [UNESP]Chiuso-Minicucci, Fernanda [UNESP]Marcolino, Larissa Doddi [UNESP]Marques, CamilaIkoma, Maura Rosane ValerioSartori, Alexandrina [UNESP]2018-12-11T17:03:13Z2018-12-11T17:03:13Z2016-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1155/2016/6765134Journal of Immunology Research, v. 2016.2314-71562314-8861http://hdl.handle.net/11449/17303410.1155/2016/67651342-s2.0-849731010292-s2.0-84973101029.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Immunology Research1,3521,352info:eu-repo/semantics/openAccess2023-11-10T06:08:45Zoai:repositorio.unesp.br:11449/173034Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-10T06:08:45Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Systemic Administration of Proteoglycan Protects BALB/c Retired Breeder Mice from Experimental Arthritis |
title |
Systemic Administration of Proteoglycan Protects BALB/c Retired Breeder Mice from Experimental Arthritis |
spellingShingle |
Systemic Administration of Proteoglycan Protects BALB/c Retired Breeder Mice from Experimental Arthritis Ishikawa, Larissa Lumi Watanabe [UNESP] |
title_short |
Systemic Administration of Proteoglycan Protects BALB/c Retired Breeder Mice from Experimental Arthritis |
title_full |
Systemic Administration of Proteoglycan Protects BALB/c Retired Breeder Mice from Experimental Arthritis |
title_fullStr |
Systemic Administration of Proteoglycan Protects BALB/c Retired Breeder Mice from Experimental Arthritis |
title_full_unstemmed |
Systemic Administration of Proteoglycan Protects BALB/c Retired Breeder Mice from Experimental Arthritis |
title_sort |
Systemic Administration of Proteoglycan Protects BALB/c Retired Breeder Mice from Experimental Arthritis |
author |
Ishikawa, Larissa Lumi Watanabe [UNESP] |
author_facet |
Ishikawa, Larissa Lumi Watanabe [UNESP] Colavite, Priscila Maria [UNESP] Fraga-Silva, Thais Fernanda De Campos [UNESP] Mimura, Luiza Ayumi Nishiyama [UNESP] Fran�a, Thais Graziela Doneg� [UNESP] Zorzella-Pezavento, Sofia Fernanda Gon�alves [UNESP] Chiuso-Minicucci, Fernanda [UNESP] Marcolino, Larissa Doddi [UNESP] Marques, Camila Ikoma, Maura Rosane Valerio Sartori, Alexandrina [UNESP] |
author_role |
author |
author2 |
Colavite, Priscila Maria [UNESP] Fraga-Silva, Thais Fernanda De Campos [UNESP] Mimura, Luiza Ayumi Nishiyama [UNESP] Fran�a, Thais Graziela Doneg� [UNESP] Zorzella-Pezavento, Sofia Fernanda Gon�alves [UNESP] Chiuso-Minicucci, Fernanda [UNESP] Marcolino, Larissa Doddi [UNESP] Marques, Camila Ikoma, Maura Rosane Valerio Sartori, Alexandrina [UNESP] |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Amaral Carvalho Foundation |
dc.contributor.author.fl_str_mv |
Ishikawa, Larissa Lumi Watanabe [UNESP] Colavite, Priscila Maria [UNESP] Fraga-Silva, Thais Fernanda De Campos [UNESP] Mimura, Luiza Ayumi Nishiyama [UNESP] Fran�a, Thais Graziela Doneg� [UNESP] Zorzella-Pezavento, Sofia Fernanda Gon�alves [UNESP] Chiuso-Minicucci, Fernanda [UNESP] Marcolino, Larissa Doddi [UNESP] Marques, Camila Ikoma, Maura Rosane Valerio Sartori, Alexandrina [UNESP] |
description |
This study was undertaken to evaluate the prophylactic potential of proteoglycan (PG) administration in experimental arthritis. Female BALB/c retired breeder mice received two (2xPG50 and 2xPG100 groups) or three (3xPG50 group) intraperitoneal doses of bovine PG (50 μg or 100 μg) every three days. A week later the animals were submitted to arthritis induction by immunization with three i.p. doses of bovine PG associated with dimethyldioctadecylammonium bromide adjuvant at intervals of 21 days. Disease severity was daily assessed after the third dose by score evaluation. The 3xPG50 group showed significant reduction in prevalence and clinical scores. This protective effect was associated with lower production of IFN-γ and IL-17 and increased production of IL-5 and IL-10 by spleen cells restimulated in vitro with PG. Even though previous PG administration restrained dendritic cells maturation this procedure did not alter the frequency of regulatory Foxp3+ T cells. Lower TNF-α and IL-6 levels and higher expression of ROR-γ and GATA-3 were detected in the paws of protected animals. A delayed-type hypersensitivity reaction confirmed specific tolerance induction. Taken together, these results indicate that previous PG inoculation determines a specific tolerogenic effect that is able to decrease severity of subsequently induced arthritis. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-01-01 2018-12-11T17:03:13Z 2018-12-11T17:03:13Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1155/2016/6765134 Journal of Immunology Research, v. 2016. 2314-7156 2314-8861 http://hdl.handle.net/11449/173034 10.1155/2016/6765134 2-s2.0-84973101029 2-s2.0-84973101029.pdf |
url |
http://dx.doi.org/10.1155/2016/6765134 http://hdl.handle.net/11449/173034 |
identifier_str_mv |
Journal of Immunology Research, v. 2016. 2314-7156 2314-8861 10.1155/2016/6765134 2-s2.0-84973101029 2-s2.0-84973101029.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Immunology Research 1,352 1,352 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799964879298232320 |