KR-12-a5 is a non-cytotoxic agent with potent antimicrobial effects against oral pathogens
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1080/08927014.2017.1370087 http://hdl.handle.net/11449/175339 |
Resumo: | This study evaluated the cytotoxicity and antimicrobial activity of analogs of cationic peptides against microorganisms associated with endodontic infections. L-929 fibroblasts were exposed to LL-37, KR-12-a5 and hBD-3–1CV and chlorhexidine (CHX, control), and cell metabolism was evaluated with MTT. The minimal inhibitory concentration (MIC) and the minimal bactericidal/fungicidal concentration (MBC/MFC) of the peptides and CHX were determined against oral pathogens associated with endodontic infections. Enterococcus faecalis and Streptococcus mutans biofilms were cultivated in bovine dentin blocks, exposed to different concentrations of the most efficient antimicrobial peptide and analyzed by confocal laser scanning microscopy. CHX and peptides affected the metabolism of L-929 at concentrations > 31.25 and 500 μg ml−1, respectively. Among the peptides, KR-12-a5 inhibited growth of both the microorganisms tested with the lowest MIC/MBC/MFC values. In addition, KR-12-a5 significantly reduced E. faecalis and S. mutans biofilms inside dentin tubules. In conclusion, KR-12-a5 is a non-cytotoxic agent with potent antimicrobial and anti-biofilm activity against oral pathogens associated with endodontic infections. |
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KR-12-a5 is a non-cytotoxic agent with potent antimicrobial effects against oral pathogensBiofilmscationic antimicrobial peptidescell culturemicrobial sensitivity testsThis study evaluated the cytotoxicity and antimicrobial activity of analogs of cationic peptides against microorganisms associated with endodontic infections. L-929 fibroblasts were exposed to LL-37, KR-12-a5 and hBD-3–1CV and chlorhexidine (CHX, control), and cell metabolism was evaluated with MTT. The minimal inhibitory concentration (MIC) and the minimal bactericidal/fungicidal concentration (MBC/MFC) of the peptides and CHX were determined against oral pathogens associated with endodontic infections. Enterococcus faecalis and Streptococcus mutans biofilms were cultivated in bovine dentin blocks, exposed to different concentrations of the most efficient antimicrobial peptide and analyzed by confocal laser scanning microscopy. CHX and peptides affected the metabolism of L-929 at concentrations > 31.25 and 500 μg ml−1, respectively. Among the peptides, KR-12-a5 inhibited growth of both the microorganisms tested with the lowest MIC/MBC/MFC values. In addition, KR-12-a5 significantly reduced E. faecalis and S. mutans biofilms inside dentin tubules. In conclusion, KR-12-a5 is a non-cytotoxic agent with potent antimicrobial and anti-biofilm activity against oral pathogens associated with endodontic infections.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)São Paulo State University (UNESP) Department of Restorative Dentistry School of DentistrySão Paulo State University (UNESP) Department of Pediatric Dentistry and Public Health School of DentistryUniversity of Campinas (UNICAMP) Department of Restorative Dentistry School of DentistryUniversity of Campinas (UNICAMP) Department of Oral Diagnosis School of DentistrySão Paulo State University (UNESP) Department of Physiology and Pathology School of DentistrySão Paulo State University (UNESP) Department of Biochemistry and Chemical Technology Institute of ChemistryDepartment of Periodontology Nove de Julho UniversitySão Paulo State University (UNESP) Department of Restorative Dentistry School of DentistrySão Paulo State University (UNESP) Department of Pediatric Dentistry and Public Health School of DentistrySão Paulo State University (UNESP) Department of Physiology and Pathology School of DentistrySão Paulo State University (UNESP) Department of Biochemistry and Chemical Technology Institute of ChemistryUniversidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Nove de Julho UniversityCaiaffa, Karina Sampaio [UNESP]Massunari, Loiane [UNESP]Danelon, Marcelle [UNESP]Abuna, Gabriel FloresBedran, Telma Blanca Lombardo [UNESP]Santos-Filho, Norival Alves [UNESP]Spolidorio, Denise Madalena Palomari [UNESP]Vizoto, Natalia LealCilli, Eduardo Maffud [UNESP]Duque, Cristiane [UNESP]2018-12-11T17:15:22Z2018-12-11T17:15:22Z2017-11-26info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article807-818application/pdfhttp://dx.doi.org/10.1080/08927014.2017.1370087Biofouling, v. 33, n. 10, p. 807-818, 2017.1029-24540892-7014http://hdl.handle.net/11449/17533910.1080/08927014.2017.13700872-s2.0-850313984302-s2.0-85031398430.pdf56518745094936170000-0002-2575-279XScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiofouling0,8350,835info:eu-repo/semantics/openAccess2023-10-18T06:04:12Zoai:repositorio.unesp.br:11449/175339Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:13:08.253415Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
KR-12-a5 is a non-cytotoxic agent with potent antimicrobial effects against oral pathogens |
title |
KR-12-a5 is a non-cytotoxic agent with potent antimicrobial effects against oral pathogens |
spellingShingle |
KR-12-a5 is a non-cytotoxic agent with potent antimicrobial effects against oral pathogens Caiaffa, Karina Sampaio [UNESP] Biofilms cationic antimicrobial peptides cell culture microbial sensitivity tests |
title_short |
KR-12-a5 is a non-cytotoxic agent with potent antimicrobial effects against oral pathogens |
title_full |
KR-12-a5 is a non-cytotoxic agent with potent antimicrobial effects against oral pathogens |
title_fullStr |
KR-12-a5 is a non-cytotoxic agent with potent antimicrobial effects against oral pathogens |
title_full_unstemmed |
KR-12-a5 is a non-cytotoxic agent with potent antimicrobial effects against oral pathogens |
title_sort |
KR-12-a5 is a non-cytotoxic agent with potent antimicrobial effects against oral pathogens |
author |
Caiaffa, Karina Sampaio [UNESP] |
author_facet |
Caiaffa, Karina Sampaio [UNESP] Massunari, Loiane [UNESP] Danelon, Marcelle [UNESP] Abuna, Gabriel Flores Bedran, Telma Blanca Lombardo [UNESP] Santos-Filho, Norival Alves [UNESP] Spolidorio, Denise Madalena Palomari [UNESP] Vizoto, Natalia Leal Cilli, Eduardo Maffud [UNESP] Duque, Cristiane [UNESP] |
author_role |
author |
author2 |
Massunari, Loiane [UNESP] Danelon, Marcelle [UNESP] Abuna, Gabriel Flores Bedran, Telma Blanca Lombardo [UNESP] Santos-Filho, Norival Alves [UNESP] Spolidorio, Denise Madalena Palomari [UNESP] Vizoto, Natalia Leal Cilli, Eduardo Maffud [UNESP] Duque, Cristiane [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Estadual de Campinas (UNICAMP) Nove de Julho University |
dc.contributor.author.fl_str_mv |
Caiaffa, Karina Sampaio [UNESP] Massunari, Loiane [UNESP] Danelon, Marcelle [UNESP] Abuna, Gabriel Flores Bedran, Telma Blanca Lombardo [UNESP] Santos-Filho, Norival Alves [UNESP] Spolidorio, Denise Madalena Palomari [UNESP] Vizoto, Natalia Leal Cilli, Eduardo Maffud [UNESP] Duque, Cristiane [UNESP] |
dc.subject.por.fl_str_mv |
Biofilms cationic antimicrobial peptides cell culture microbial sensitivity tests |
topic |
Biofilms cationic antimicrobial peptides cell culture microbial sensitivity tests |
description |
This study evaluated the cytotoxicity and antimicrobial activity of analogs of cationic peptides against microorganisms associated with endodontic infections. L-929 fibroblasts were exposed to LL-37, KR-12-a5 and hBD-3–1CV and chlorhexidine (CHX, control), and cell metabolism was evaluated with MTT. The minimal inhibitory concentration (MIC) and the minimal bactericidal/fungicidal concentration (MBC/MFC) of the peptides and CHX were determined against oral pathogens associated with endodontic infections. Enterococcus faecalis and Streptococcus mutans biofilms were cultivated in bovine dentin blocks, exposed to different concentrations of the most efficient antimicrobial peptide and analyzed by confocal laser scanning microscopy. CHX and peptides affected the metabolism of L-929 at concentrations > 31.25 and 500 μg ml−1, respectively. Among the peptides, KR-12-a5 inhibited growth of both the microorganisms tested with the lowest MIC/MBC/MFC values. In addition, KR-12-a5 significantly reduced E. faecalis and S. mutans biofilms inside dentin tubules. In conclusion, KR-12-a5 is a non-cytotoxic agent with potent antimicrobial and anti-biofilm activity against oral pathogens associated with endodontic infections. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-11-26 2018-12-11T17:15:22Z 2018-12-11T17:15:22Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1080/08927014.2017.1370087 Biofouling, v. 33, n. 10, p. 807-818, 2017. 1029-2454 0892-7014 http://hdl.handle.net/11449/175339 10.1080/08927014.2017.1370087 2-s2.0-85031398430 2-s2.0-85031398430.pdf 5651874509493617 0000-0002-2575-279X |
url |
http://dx.doi.org/10.1080/08927014.2017.1370087 http://hdl.handle.net/11449/175339 |
identifier_str_mv |
Biofouling, v. 33, n. 10, p. 807-818, 2017. 1029-2454 0892-7014 10.1080/08927014.2017.1370087 2-s2.0-85031398430 2-s2.0-85031398430.pdf 5651874509493617 0000-0002-2575-279X |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biofouling 0,835 0,835 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
807-818 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128481305821184 |