Bicompatibilidade do azul de metileno em diferentes PHs

• Autor(a) principal: Fioravante, Amanda Pontes [UNESP]
• Data de Publicação: 2017
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/156628; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2018-06-12/000900566.pdf
• topic: Teste de materiais; Tela subcutânea; Concentração de íons de hidrogênio; Azul de Metileno; Materials Testing

The aim of this study was to investigate the biocompatibility of methylene blue at different pH levels through the method of implantation in subcutaneous tissue. Eighty-four sterilized polyethylene tubes were allocated in the subcutaneous tissue of 21 rats, each one receiving 4 tubes, set into 4 groups: group tube (G-T) - empty tube; Fibrin group (G-F) - tube filled with fibrin sponge; Group Methylene Blue pH 7 (G-MB/pH7) - tube filled with fibrin sponge soaked by methylene blue (100μg/ml) at pH 7.0; Group Methylene Blue pH 1 (G-MB/pH1) - tube filled with fibrin sponge and soaked by methylene blue (100μg / ml) at pH 1.0. After 7, 15 and 30 days, 7 animals from each group were euthanized and the tubes involved by the surrounding tissue were removed and fixed with 4% buffered formaldehyde solution. The collected pieces were processed and histological sections (4 μm) were stained with hematoxylin and eosin and analyzed by light microscopy. Scores were assigned to analysis of histopathologic parameters. The results were statistically analyzed by the Kruskal Wallis test (p ≤ 0.05). At 7 and 30 days the G-MB/pH 1 group showed no significant difference in the G-T control group, while G-MB/pH 7 had a significant increase on tissue reaction, also when compared to G-T. At 15 days there was no statistical difference between the groups. Within the limits of this study, it is concluded that methylene blue at pH 1.0 provides better biocompatibility than at pH 7.0