Chemosensory Gene Expression for Two Closely Relative Species Rhodnius robustus and R. prolixus (Hemiptera, Reduviidade, Triatominae) Vectors of Chagas Disease
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3389/fevo.2021.725504 http://hdl.handle.net/11449/222688 |
Resumo: | Two closely related species, Rhodnius prolixus and Rhodnius robustus, are the vectors of Trypanosoma cruzi, which is the causative agent of Chagas disease, but clearly exhibit clear-cut differences in their ecological behavior. R. prolixus is considered as a domiciliated species, whereas R. robustus only sporadically visits human houses in Amazonia. We performed a chemosensory gene expression study via RNA-sequencing (RNA-seq) for the two species and also included a laboratory introgressed R. robustus strain. We built an assembled transcriptome for each sample and for both sexes and compiled all in a reference transcriptome for a differential gene expression study. Because the genes specifically expressed in one condition and not expressed in another may also reflect differences in the adaptation of organisms, a comparative study of the presence/absence of transcripts was also performed for the chemosensory transcripts, namely chemosensory proteins (CSPs), odorant-binding proteins (OBPs), odorant receptors (ORs), gustatory receptors (GRs), and ionotropic receptors (IRs), as well as takeout (TO) transcripts because TO proteins have been proposed to be associated with chemosensory perception in both olfactory and taste systems. In this study, 12 novel TO transcripts from the R. prolixus genome were annotated. Among the 199 transcripts, out of interest, annotated in this study, 93% were conserved between R. prolixus and the sylvatic R. robustus. Moreover, 10 transcripts out of interest were specifically expressed in one sex and absent in another. Three chemosensory transcripts were found to be expressed only in the reared R. prolixus (CSP19, OBP9, and OR89) and only one in sylvatic R. robustus (OR22). A large set of transcripts were found to be differentially expressed (DE) between males and females (1,630), with a majority of them (83%) overexpressed in males. Between environmental conditions, 8,596 transcripts were DE, with most (67%) overexpressed in the sylvatic R. robustus samples, including 17 chemosensory transcripts (4 CSPs, 1 OBP, 5 ORs, 1 GR, 4 IR, and 2 TO), but 4 genes (OBP19, OR13, OR40, and OR79) were overexpressed in the reared samples. |
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Chemosensory Gene Expression for Two Closely Relative Species Rhodnius robustus and R. prolixus (Hemiptera, Reduviidade, Triatominae) Vectors of Chagas DiseaseChagas diseasechemosensory proteins (CSP)differential expressionGR)odorant and gustatory receptors (ORodorant binding protein (OBP)Rhodnius prolixusRhodnius robustusTwo closely related species, Rhodnius prolixus and Rhodnius robustus, are the vectors of Trypanosoma cruzi, which is the causative agent of Chagas disease, but clearly exhibit clear-cut differences in their ecological behavior. R. prolixus is considered as a domiciliated species, whereas R. robustus only sporadically visits human houses in Amazonia. We performed a chemosensory gene expression study via RNA-sequencing (RNA-seq) for the two species and also included a laboratory introgressed R. robustus strain. We built an assembled transcriptome for each sample and for both sexes and compiled all in a reference transcriptome for a differential gene expression study. Because the genes specifically expressed in one condition and not expressed in another may also reflect differences in the adaptation of organisms, a comparative study of the presence/absence of transcripts was also performed for the chemosensory transcripts, namely chemosensory proteins (CSPs), odorant-binding proteins (OBPs), odorant receptors (ORs), gustatory receptors (GRs), and ionotropic receptors (IRs), as well as takeout (TO) transcripts because TO proteins have been proposed to be associated with chemosensory perception in both olfactory and taste systems. In this study, 12 novel TO transcripts from the R. prolixus genome were annotated. Among the 199 transcripts, out of interest, annotated in this study, 93% were conserved between R. prolixus and the sylvatic R. robustus. Moreover, 10 transcripts out of interest were specifically expressed in one sex and absent in another. Three chemosensory transcripts were found to be expressed only in the reared R. prolixus (CSP19, OBP9, and OR89) and only one in sylvatic R. robustus (OR22). A large set of transcripts were found to be differentially expressed (DE) between males and females (1,630), with a majority of them (83%) overexpressed in males. Between environmental conditions, 8,596 transcripts were DE, with most (67%) overexpressed in the sylvatic R. robustus samples, including 17 chemosensory transcripts (4 CSPs, 1 OBP, 5 ORs, 1 GR, 4 IR, and 2 TO), but 4 genes (OBP19, OR13, OR40, and OR79) were overexpressed in the reared samples.Agence Nationale de la RechercheUniversité Paris-SaclayUniversité Paris Saclay CNRS IRD UMR EGCE Evolution Génomes Comportement et EcologieINRAE Sorbonne Université CNRS IRD UPEC Université de Paris Institute of Ecology and Environmental Sciences of ParisInstituto de Biologia Universidade Federal da Bahia (UFBA)Laboratoire Hospitalier Universitaire de Parasitologie et Mycologie (LHUPM) UFR de Médecine Université des Antilles et de la Guyane Equipe EAMuséum National d'Histoire Naturelle Département Systématique Evolution (Entomologie)Aix Marseille Université IRD AP-HM SSA VITROME IHU-Méditerranée InfectionFaculdade de Ciências Farmacêuticas Universidade Estadual Paulista (Unesp)Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista (Unesp)Comportement et EcologieInstitute of Ecology and Environmental Sciences of ParisUniversidade Federal da Bahia (UFBA)Equipe EADépartement Systématique Evolution (Entomologie)IHU-Méditerranée InfectionUniversidade Estadual Paulista (UNESP)Marchant, AxelleMougel, FlorenceJacquin-Joly, EmmanuelleAlmeida, Carlos E.Blanchet, DenisBérenger, Jean-Michelda Rosa, João A. [UNESP]Harry, Myriam2022-04-28T19:46:06Z2022-04-28T19:46:06Z2021-10-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3389/fevo.2021.725504Frontiers in Ecology and Evolution, v. 9.2296-701Xhttp://hdl.handle.net/11449/22268810.3389/fevo.2021.7255042-s2.0-85117463386Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers in Ecology and Evolutioninfo:eu-repo/semantics/openAccess2022-04-28T19:46:06Zoai:repositorio.unesp.br:11449/222688Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:07:50.995487Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Chemosensory Gene Expression for Two Closely Relative Species Rhodnius robustus and R. prolixus (Hemiptera, Reduviidade, Triatominae) Vectors of Chagas Disease |
title |
Chemosensory Gene Expression for Two Closely Relative Species Rhodnius robustus and R. prolixus (Hemiptera, Reduviidade, Triatominae) Vectors of Chagas Disease |
spellingShingle |
Chemosensory Gene Expression for Two Closely Relative Species Rhodnius robustus and R. prolixus (Hemiptera, Reduviidade, Triatominae) Vectors of Chagas Disease Marchant, Axelle Chagas disease chemosensory proteins (CSP) differential expression GR) odorant and gustatory receptors (OR odorant binding protein (OBP) Rhodnius prolixus Rhodnius robustus |
title_short |
Chemosensory Gene Expression for Two Closely Relative Species Rhodnius robustus and R. prolixus (Hemiptera, Reduviidade, Triatominae) Vectors of Chagas Disease |
title_full |
Chemosensory Gene Expression for Two Closely Relative Species Rhodnius robustus and R. prolixus (Hemiptera, Reduviidade, Triatominae) Vectors of Chagas Disease |
title_fullStr |
Chemosensory Gene Expression for Two Closely Relative Species Rhodnius robustus and R. prolixus (Hemiptera, Reduviidade, Triatominae) Vectors of Chagas Disease |
title_full_unstemmed |
Chemosensory Gene Expression for Two Closely Relative Species Rhodnius robustus and R. prolixus (Hemiptera, Reduviidade, Triatominae) Vectors of Chagas Disease |
title_sort |
Chemosensory Gene Expression for Two Closely Relative Species Rhodnius robustus and R. prolixus (Hemiptera, Reduviidade, Triatominae) Vectors of Chagas Disease |
author |
Marchant, Axelle |
author_facet |
Marchant, Axelle Mougel, Florence Jacquin-Joly, Emmanuelle Almeida, Carlos E. Blanchet, Denis Bérenger, Jean-Michel da Rosa, João A. [UNESP] Harry, Myriam |
author_role |
author |
author2 |
Mougel, Florence Jacquin-Joly, Emmanuelle Almeida, Carlos E. Blanchet, Denis Bérenger, Jean-Michel da Rosa, João A. [UNESP] Harry, Myriam |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Comportement et Ecologie Institute of Ecology and Environmental Sciences of Paris Universidade Federal da Bahia (UFBA) Equipe EA Département Systématique Evolution (Entomologie) IHU-Méditerranée Infection Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Marchant, Axelle Mougel, Florence Jacquin-Joly, Emmanuelle Almeida, Carlos E. Blanchet, Denis Bérenger, Jean-Michel da Rosa, João A. [UNESP] Harry, Myriam |
dc.subject.por.fl_str_mv |
Chagas disease chemosensory proteins (CSP) differential expression GR) odorant and gustatory receptors (OR odorant binding protein (OBP) Rhodnius prolixus Rhodnius robustus |
topic |
Chagas disease chemosensory proteins (CSP) differential expression GR) odorant and gustatory receptors (OR odorant binding protein (OBP) Rhodnius prolixus Rhodnius robustus |
description |
Two closely related species, Rhodnius prolixus and Rhodnius robustus, are the vectors of Trypanosoma cruzi, which is the causative agent of Chagas disease, but clearly exhibit clear-cut differences in their ecological behavior. R. prolixus is considered as a domiciliated species, whereas R. robustus only sporadically visits human houses in Amazonia. We performed a chemosensory gene expression study via RNA-sequencing (RNA-seq) for the two species and also included a laboratory introgressed R. robustus strain. We built an assembled transcriptome for each sample and for both sexes and compiled all in a reference transcriptome for a differential gene expression study. Because the genes specifically expressed in one condition and not expressed in another may also reflect differences in the adaptation of organisms, a comparative study of the presence/absence of transcripts was also performed for the chemosensory transcripts, namely chemosensory proteins (CSPs), odorant-binding proteins (OBPs), odorant receptors (ORs), gustatory receptors (GRs), and ionotropic receptors (IRs), as well as takeout (TO) transcripts because TO proteins have been proposed to be associated with chemosensory perception in both olfactory and taste systems. In this study, 12 novel TO transcripts from the R. prolixus genome were annotated. Among the 199 transcripts, out of interest, annotated in this study, 93% were conserved between R. prolixus and the sylvatic R. robustus. Moreover, 10 transcripts out of interest were specifically expressed in one sex and absent in another. Three chemosensory transcripts were found to be expressed only in the reared R. prolixus (CSP19, OBP9, and OR89) and only one in sylvatic R. robustus (OR22). A large set of transcripts were found to be differentially expressed (DE) between males and females (1,630), with a majority of them (83%) overexpressed in males. Between environmental conditions, 8,596 transcripts were DE, with most (67%) overexpressed in the sylvatic R. robustus samples, including 17 chemosensory transcripts (4 CSPs, 1 OBP, 5 ORs, 1 GR, 4 IR, and 2 TO), but 4 genes (OBP19, OR13, OR40, and OR79) were overexpressed in the reared samples. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-10-06 2022-04-28T19:46:06Z 2022-04-28T19:46:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3389/fevo.2021.725504 Frontiers in Ecology and Evolution, v. 9. 2296-701X http://hdl.handle.net/11449/222688 10.3389/fevo.2021.725504 2-s2.0-85117463386 |
url |
http://dx.doi.org/10.3389/fevo.2021.725504 http://hdl.handle.net/11449/222688 |
identifier_str_mv |
Frontiers in Ecology and Evolution, v. 9. 2296-701X 10.3389/fevo.2021.725504 2-s2.0-85117463386 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Frontiers in Ecology and Evolution |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128759172169728 |