Meiotic self-pairing of the psalidodon (Characiformes, characidae) iso-b chromosome: A successful perpetuation mechanism
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1678-4685-GMB-2021-0084 http://hdl.handle.net/11449/229870 |
Resumo: | B chromosomes are non-essential additional genomic elements present in several animal and plant species. In fishes, species of the genus Psalidodon (Characiformes, Characidae) harbor great karyotype diversity, and multiple populations carry different types of non-essential B chromosomes. This study analyzed how the dispensable supernumerary B chromosome of Psalidodon paranae behaves during meiosis to overcome checkpoints and express its own meiosis-specific genes. We visualized the synaptonemal complexes of P. paranae individuals with zero, one, or two B chromosomes using immunodetection with anti-medaka SYCP3 antibody and fluorescence in situ hybridization with a (CA)15 microsatellite probe. Our results showed that B chromosomes self-pair in cells containing only one B chromosome. In cells with two identical B chromosomes, these elements remain as separate synaptonemal complexes or close self-paired elements in the nucleus territory. Overall, we reveal that B chromosomes can escape meiotic silencing of unsynapsed chromatin through a self-pairing process, allowing expression of their own genes to facilitate regular meiosis resulting in fertile individuals. This behavior, also seen in other congeneric species, might be related to their maintenance throughout the evolutionary history of Psalidodon. |
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Meiotic self-pairing of the psalidodon (Characiformes, characidae) iso-b chromosome: A successful perpetuation mechanismFish meiosisMeiotic silencing of unsynapsed chromatin (MSUC)SYCP3Synaptonemal complexTetra fishB chromosomes are non-essential additional genomic elements present in several animal and plant species. In fishes, species of the genus Psalidodon (Characiformes, Characidae) harbor great karyotype diversity, and multiple populations carry different types of non-essential B chromosomes. This study analyzed how the dispensable supernumerary B chromosome of Psalidodon paranae behaves during meiosis to overcome checkpoints and express its own meiosis-specific genes. We visualized the synaptonemal complexes of P. paranae individuals with zero, one, or two B chromosomes using immunodetection with anti-medaka SYCP3 antibody and fluorescence in situ hybridization with a (CA)15 microsatellite probe. Our results showed that B chromosomes self-pair in cells containing only one B chromosome. In cells with two identical B chromosomes, these elements remain as separate synaptonemal complexes or close self-paired elements in the nucleus territory. Overall, we reveal that B chromosomes can escape meiotic silencing of unsynapsed chromatin through a self-pairing process, allowing expression of their own genes to facilitate regular meiosis resulting in fertile individuals. This behavior, also seen in other congeneric species, might be related to their maintenance throughout the evolutionary history of Psalidodon.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Estadual Paulista (UNESP) Instituto de Biociências de Botucatu Departamento de Biologia Estrutural e FuncionalUniversidad de La Serena Instituto de Investigación Multidisciplinar en Ciencia y TecnologíaUniversidad de La Serena Departamento de Biología Laboratorio de Genética y Citogenética VegetalHokkaido University Faculty of Science Department of Biological Sciences Laboratory of Reproductive & Developmental BiologyUniversidade Estadual Paulista (UNESP) Faculdade de Ciências Departamento de Ciências BiológicasUniversidade Federal de São Carlos (UFSCAR) Departamento de Genética e EvoluçãoUniversidade Estadual de Ponta Grossa (UEPG) Departamento de Biologia Estrutural Molecular e GenéticaUniversidade Estadual Paulista (UNESP) Instituto de Biociências de Botucatu Departamento de Biologia Estrutural e FuncionalUniversidade Estadual Paulista (UNESP) Faculdade de Ciências Departamento de Ciências BiológicasFAPESP: 2013/24143-5FAPESP: 2014/09634-5FAPESP: 2014/26508-3FAPESP: 2016/09204-6FAPESP: 2017/15608-5FAPESP: 2017/22447-8FAPESP: 2018/20610-1CNPq: 306054/2006-0Universidade Estadual Paulista (UNESP)Instituto de Investigación Multidisciplinar en Ciencia y TecnologíaLaboratorio de Genética y Citogenética VegetalLaboratory of Reproductive & Developmental BiologyUniversidade Federal de São Carlos (UFSCar)Universidade Estadual de Ponta Grossa (UEPG)Silva, Duílio Mazzoni Zerbinato de Andrade [UNESP]Araya-Jaime, CristianYamashita, MasakaneVidal, Mateus Rossetto [UNESP]Oliveira, Claudio [UNESP]Porto-Foresti, Fábio [UNESP]Artoni, Roberto FerreiraForesti, Fausto [UNESP]2022-04-29T08:36:19Z2022-04-29T08:36:19Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1590/1678-4685-GMB-2021-0084Genetics and Molecular Biology, v. 44, n. 3, 2021.1678-46851415-4757http://hdl.handle.net/11449/22987010.1590/1678-4685-GMB-2021-00842-s2.0-85118935123Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Biologyinfo:eu-repo/semantics/openAccess2024-04-23T15:23:29Zoai:repositorio.unesp.br:11449/229870Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-23T15:23:29Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Meiotic self-pairing of the psalidodon (Characiformes, characidae) iso-b chromosome: A successful perpetuation mechanism |
title |
Meiotic self-pairing of the psalidodon (Characiformes, characidae) iso-b chromosome: A successful perpetuation mechanism |
spellingShingle |
Meiotic self-pairing of the psalidodon (Characiformes, characidae) iso-b chromosome: A successful perpetuation mechanism Silva, Duílio Mazzoni Zerbinato de Andrade [UNESP] Fish meiosis Meiotic silencing of unsynapsed chromatin (MSUC) SYCP3 Synaptonemal complex Tetra fish |
title_short |
Meiotic self-pairing of the psalidodon (Characiformes, characidae) iso-b chromosome: A successful perpetuation mechanism |
title_full |
Meiotic self-pairing of the psalidodon (Characiformes, characidae) iso-b chromosome: A successful perpetuation mechanism |
title_fullStr |
Meiotic self-pairing of the psalidodon (Characiformes, characidae) iso-b chromosome: A successful perpetuation mechanism |
title_full_unstemmed |
Meiotic self-pairing of the psalidodon (Characiformes, characidae) iso-b chromosome: A successful perpetuation mechanism |
title_sort |
Meiotic self-pairing of the psalidodon (Characiformes, characidae) iso-b chromosome: A successful perpetuation mechanism |
author |
Silva, Duílio Mazzoni Zerbinato de Andrade [UNESP] |
author_facet |
Silva, Duílio Mazzoni Zerbinato de Andrade [UNESP] Araya-Jaime, Cristian Yamashita, Masakane Vidal, Mateus Rossetto [UNESP] Oliveira, Claudio [UNESP] Porto-Foresti, Fábio [UNESP] Artoni, Roberto Ferreira Foresti, Fausto [UNESP] |
author_role |
author |
author2 |
Araya-Jaime, Cristian Yamashita, Masakane Vidal, Mateus Rossetto [UNESP] Oliveira, Claudio [UNESP] Porto-Foresti, Fábio [UNESP] Artoni, Roberto Ferreira Foresti, Fausto [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Instituto de Investigación Multidisciplinar en Ciencia y Tecnología Laboratorio de Genética y Citogenética Vegetal Laboratory of Reproductive & Developmental Biology Universidade Federal de São Carlos (UFSCar) Universidade Estadual de Ponta Grossa (UEPG) |
dc.contributor.author.fl_str_mv |
Silva, Duílio Mazzoni Zerbinato de Andrade [UNESP] Araya-Jaime, Cristian Yamashita, Masakane Vidal, Mateus Rossetto [UNESP] Oliveira, Claudio [UNESP] Porto-Foresti, Fábio [UNESP] Artoni, Roberto Ferreira Foresti, Fausto [UNESP] |
dc.subject.por.fl_str_mv |
Fish meiosis Meiotic silencing of unsynapsed chromatin (MSUC) SYCP3 Synaptonemal complex Tetra fish |
topic |
Fish meiosis Meiotic silencing of unsynapsed chromatin (MSUC) SYCP3 Synaptonemal complex Tetra fish |
description |
B chromosomes are non-essential additional genomic elements present in several animal and plant species. In fishes, species of the genus Psalidodon (Characiformes, Characidae) harbor great karyotype diversity, and multiple populations carry different types of non-essential B chromosomes. This study analyzed how the dispensable supernumerary B chromosome of Psalidodon paranae behaves during meiosis to overcome checkpoints and express its own meiosis-specific genes. We visualized the synaptonemal complexes of P. paranae individuals with zero, one, or two B chromosomes using immunodetection with anti-medaka SYCP3 antibody and fluorescence in situ hybridization with a (CA)15 microsatellite probe. Our results showed that B chromosomes self-pair in cells containing only one B chromosome. In cells with two identical B chromosomes, these elements remain as separate synaptonemal complexes or close self-paired elements in the nucleus territory. Overall, we reveal that B chromosomes can escape meiotic silencing of unsynapsed chromatin through a self-pairing process, allowing expression of their own genes to facilitate regular meiosis resulting in fertile individuals. This behavior, also seen in other congeneric species, might be related to their maintenance throughout the evolutionary history of Psalidodon. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 2022-04-29T08:36:19Z 2022-04-29T08:36:19Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1678-4685-GMB-2021-0084 Genetics and Molecular Biology, v. 44, n. 3, 2021. 1678-4685 1415-4757 http://hdl.handle.net/11449/229870 10.1590/1678-4685-GMB-2021-0084 2-s2.0-85118935123 |
url |
http://dx.doi.org/10.1590/1678-4685-GMB-2021-0084 http://hdl.handle.net/11449/229870 |
identifier_str_mv |
Genetics and Molecular Biology, v. 44, n. 3, 2021. 1678-4685 1415-4757 10.1590/1678-4685-GMB-2021-0084 2-s2.0-85118935123 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genetics and Molecular Biology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803649734446940160 |