Nitric oxide in the insular cortex modulates baroreflex responses in a cGMP-independent pathway

Detalhes bibliográficos
Autor(a) principal: Ferreira-Junior, Nilson C.
Data de Publicação: 2020
Outros Autores: Crestani, Carlos C. [UNESP], Lagatta, Davi C., Resstel, Leonardo B.M., Correa, Fernando M.A., Alves, Fernando H.F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.brainres.2020.147037
http://hdl.handle.net/11449/199202
Resumo: Insular cortex is a brain structure involved in the modulation of autonomic activity and cardiovascular function. The nitric oxide/cyclic guanosine-3′,5′-monophosphate pathway is a prominent signaling mechanism in the central nervous system, controlling behavioral and physiological responses. Nevertheless, despite evidence regarding the presence of nitric oxide-synthesizing neurons in the insular cortex, its role in the control of autonomic and cardiovascular function has never been reported. Thus, the present study aimed to investigate the involvement of nitric oxide/cyclic guanosine-3′,5′-monophosphate pathway mediated by neuronal nitric oxide synthase (nNOS) activation within the insular cortex in the modulation of baroreflex responses in unanesthetized rats. For this, we evaluated the effect of bilateral microinjection of either the nitric oxide scavenger carboxy-PTIO, the selective neuronal nitric oxide synthase inhibitor Nω-Propyl-L-arginine or the soluble guanylate cyclase inhibitor ODQ into the insular cortex on the bradycardia evoked by blood pressure increases in response to intravenous infusion of phenylephrine, and the tachycardia caused by blood pressure decreases evoked by intravenous infusion of sodium nitroprusside. Bilateral microinjection of either NPLA or carboxy-PTIO into the insular cortex increased the reflex bradycardic response, whereas the reflex tachycardia was decreased by these treatments. Bilateral microinjection of the soluble guanylate cyclase inhibitor into the insular cortex did not affect any parameter of baroreflex function evaluated. Overall, our findings provide evidence that insular cortex nitrergic signaling, acting via neuronal nitric oxide synthase, plays a prominent role in control of baroreflex function. However, control of reflex responses seems to be independent of soluble guanylate cyclase activation.
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spelling Nitric oxide in the insular cortex modulates baroreflex responses in a cGMP-independent pathwayBaroreflexGMPcInsular cortexNitric oxideInsular cortex is a brain structure involved in the modulation of autonomic activity and cardiovascular function. The nitric oxide/cyclic guanosine-3′,5′-monophosphate pathway is a prominent signaling mechanism in the central nervous system, controlling behavioral and physiological responses. Nevertheless, despite evidence regarding the presence of nitric oxide-synthesizing neurons in the insular cortex, its role in the control of autonomic and cardiovascular function has never been reported. Thus, the present study aimed to investigate the involvement of nitric oxide/cyclic guanosine-3′,5′-monophosphate pathway mediated by neuronal nitric oxide synthase (nNOS) activation within the insular cortex in the modulation of baroreflex responses in unanesthetized rats. For this, we evaluated the effect of bilateral microinjection of either the nitric oxide scavenger carboxy-PTIO, the selective neuronal nitric oxide synthase inhibitor Nω-Propyl-L-arginine or the soluble guanylate cyclase inhibitor ODQ into the insular cortex on the bradycardia evoked by blood pressure increases in response to intravenous infusion of phenylephrine, and the tachycardia caused by blood pressure decreases evoked by intravenous infusion of sodium nitroprusside. Bilateral microinjection of either NPLA or carboxy-PTIO into the insular cortex increased the reflex bradycardic response, whereas the reflex tachycardia was decreased by these treatments. Bilateral microinjection of the soluble guanylate cyclase inhibitor into the insular cortex did not affect any parameter of baroreflex function evaluated. Overall, our findings provide evidence that insular cortex nitrergic signaling, acting via neuronal nitric oxide synthase, plays a prominent role in control of baroreflex function. However, control of reflex responses seems to be independent of soluble guanylate cyclase activation.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Department of Pharmacology School of Medicine of Ribeirão Preto University of São PauloLaboratory of Pharmacology School of Pharmaceutical Sciences São Paulo State University (UNESP)Department of Health Sciences Faculty of Medicine – Federal University of LavrasLaboratory of Pharmacology School of Pharmaceutical Sciences São Paulo State University (UNESP)CNPq: 141992/2016-6FAPESP: 2015/05922-9CNPq: 456405/2014-3FAPEMIG: APQ-01316-16Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Faculty of Medicine – Federal University of LavrasFerreira-Junior, Nilson C.Crestani, Carlos C. [UNESP]Lagatta, Davi C.Resstel, Leonardo B.M.Correa, Fernando M.A.Alves, Fernando H.F.2020-12-12T01:33:30Z2020-12-12T01:33:30Z2020-11-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.brainres.2020.147037Brain Research, v. 1747.1872-62400006-8993http://hdl.handle.net/11449/19920210.1016/j.brainres.2020.1470372-s2.0-85088929328Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrain Researchinfo:eu-repo/semantics/openAccess2021-10-23T04:45:41Zoai:repositorio.unesp.br:11449/199202Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:15:34.842293Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Nitric oxide in the insular cortex modulates baroreflex responses in a cGMP-independent pathway
title Nitric oxide in the insular cortex modulates baroreflex responses in a cGMP-independent pathway
spellingShingle Nitric oxide in the insular cortex modulates baroreflex responses in a cGMP-independent pathway
Ferreira-Junior, Nilson C.
Baroreflex
GMPc
Insular cortex
Nitric oxide
title_short Nitric oxide in the insular cortex modulates baroreflex responses in a cGMP-independent pathway
title_full Nitric oxide in the insular cortex modulates baroreflex responses in a cGMP-independent pathway
title_fullStr Nitric oxide in the insular cortex modulates baroreflex responses in a cGMP-independent pathway
title_full_unstemmed Nitric oxide in the insular cortex modulates baroreflex responses in a cGMP-independent pathway
title_sort Nitric oxide in the insular cortex modulates baroreflex responses in a cGMP-independent pathway
author Ferreira-Junior, Nilson C.
author_facet Ferreira-Junior, Nilson C.
Crestani, Carlos C. [UNESP]
Lagatta, Davi C.
Resstel, Leonardo B.M.
Correa, Fernando M.A.
Alves, Fernando H.F.
author_role author
author2 Crestani, Carlos C. [UNESP]
Lagatta, Davi C.
Resstel, Leonardo B.M.
Correa, Fernando M.A.
Alves, Fernando H.F.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Estadual Paulista (Unesp)
Faculty of Medicine – Federal University of Lavras
dc.contributor.author.fl_str_mv Ferreira-Junior, Nilson C.
Crestani, Carlos C. [UNESP]
Lagatta, Davi C.
Resstel, Leonardo B.M.
Correa, Fernando M.A.
Alves, Fernando H.F.
dc.subject.por.fl_str_mv Baroreflex
GMPc
Insular cortex
Nitric oxide
topic Baroreflex
GMPc
Insular cortex
Nitric oxide
description Insular cortex is a brain structure involved in the modulation of autonomic activity and cardiovascular function. The nitric oxide/cyclic guanosine-3′,5′-monophosphate pathway is a prominent signaling mechanism in the central nervous system, controlling behavioral and physiological responses. Nevertheless, despite evidence regarding the presence of nitric oxide-synthesizing neurons in the insular cortex, its role in the control of autonomic and cardiovascular function has never been reported. Thus, the present study aimed to investigate the involvement of nitric oxide/cyclic guanosine-3′,5′-monophosphate pathway mediated by neuronal nitric oxide synthase (nNOS) activation within the insular cortex in the modulation of baroreflex responses in unanesthetized rats. For this, we evaluated the effect of bilateral microinjection of either the nitric oxide scavenger carboxy-PTIO, the selective neuronal nitric oxide synthase inhibitor Nω-Propyl-L-arginine or the soluble guanylate cyclase inhibitor ODQ into the insular cortex on the bradycardia evoked by blood pressure increases in response to intravenous infusion of phenylephrine, and the tachycardia caused by blood pressure decreases evoked by intravenous infusion of sodium nitroprusside. Bilateral microinjection of either NPLA or carboxy-PTIO into the insular cortex increased the reflex bradycardic response, whereas the reflex tachycardia was decreased by these treatments. Bilateral microinjection of the soluble guanylate cyclase inhibitor into the insular cortex did not affect any parameter of baroreflex function evaluated. Overall, our findings provide evidence that insular cortex nitrergic signaling, acting via neuronal nitric oxide synthase, plays a prominent role in control of baroreflex function. However, control of reflex responses seems to be independent of soluble guanylate cyclase activation.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T01:33:30Z
2020-12-12T01:33:30Z
2020-11-15
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.brainres.2020.147037
Brain Research, v. 1747.
1872-6240
0006-8993
http://hdl.handle.net/11449/199202
10.1016/j.brainres.2020.147037
2-s2.0-85088929328
url http://dx.doi.org/10.1016/j.brainres.2020.147037
http://hdl.handle.net/11449/199202
identifier_str_mv Brain Research, v. 1747.
1872-6240
0006-8993
10.1016/j.brainres.2020.147037
2-s2.0-85088929328
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brain Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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