Staphylococcus epidermidis and Staphylococcus haemolyticus: molecular detection of cytotoxin and enterotoxin genes

Detalhes bibliográficos
Autor(a) principal: Pinheiro, Luiza [UNESP]
Data de Publicação: 2015
Outros Autores: Brito, Carla Ivo [UNESP], Oliveira, Adilson de [UNESP], Martins, Patrícia Yoshida Faccioli [UNESP], Pereira, Valéria Cataneli [UNESP], Cunha, Maria de Lourdes Ribeiro de Souza da [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3390/toxins7093688
http://hdl.handle.net/11449/131230
Resumo: Although opportunistic pathogens, coagulase-negative staphylococci (CoNS), including Staphylococcus epidermidis and Staphylococcus haemolyticus, have long been regarded as avirulent organisms. The role of toxins in the development of infections caused by CoNS is still controversial. The objective of this study was to characterize the presence of enterotoxin and cytotoxin genes in S. epidermidis and S. haemolyticus isolates obtained from blood cultures. Cytotoxin genes were detected by PCR using novel species-specific primers. Among the 85 S. epidermidis and 84 S. haemolyticus isolates, 95.3% and 79.8%, respectively, carried at least one enterotoxin gene. The most frequent enterotoxin genes were sea (53.3%), seg (64.5%) and sei (67.5%). The seg gene was positively associated with S. epidermidis (p = 0.02), and this species was more toxigenic than S. haemolyticus. The hla/yidD gene was detected in 92.9% of S. epidermidis and the hla gene in 91.7% of S. haemolyticus isolates; hlb was detected in 92.9% of the S. epidermidis isolates and hld in 95.3%. Nosocomial Staphylococcus epidermidis and S. haemolyticus isolates exhibited a high toxigenic potential, mainly producing the non-classical enterotoxins seg and sei. The previously unreported detection of hla/yidD and hlb in S. epidermidis and S. haemolyticus using species-specific primers showed that these hemolysin genes differ between CoNS species and that they are highly frequent in blood culture isolates.
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spelling Staphylococcus epidermidis and Staphylococcus haemolyticus: molecular detection of cytotoxin and enterotoxin genesStaphylococcus epidermidisStaphylococcus haemolyticusCytotoxinsEnterotoxinsAlthough opportunistic pathogens, coagulase-negative staphylococci (CoNS), including Staphylococcus epidermidis and Staphylococcus haemolyticus, have long been regarded as avirulent organisms. The role of toxins in the development of infections caused by CoNS is still controversial. The objective of this study was to characterize the presence of enterotoxin and cytotoxin genes in S. epidermidis and S. haemolyticus isolates obtained from blood cultures. Cytotoxin genes were detected by PCR using novel species-specific primers. Among the 85 S. epidermidis and 84 S. haemolyticus isolates, 95.3% and 79.8%, respectively, carried at least one enterotoxin gene. The most frequent enterotoxin genes were sea (53.3%), seg (64.5%) and sei (67.5%). The seg gene was positively associated with S. epidermidis (p = 0.02), and this species was more toxigenic than S. haemolyticus. The hla/yidD gene was detected in 92.9% of S. epidermidis and the hla gene in 91.7% of S. haemolyticus isolates; hlb was detected in 92.9% of the S. epidermidis isolates and hld in 95.3%. Nosocomial Staphylococcus epidermidis and S. haemolyticus isolates exhibited a high toxigenic potential, mainly producing the non-classical enterotoxins seg and sei. The previously unreported detection of hla/yidD and hlb in S. epidermidis and S. haemolyticus using species-specific primers showed that these hemolysin genes differ between CoNS species and that they are highly frequent in blood culture isolates.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Estadual Paulista, Departamento de Microbiologia e Imunologia, Instituto de Biociências de BotucatuFAPESP: 2011/15396-1CNPq: 304729/2014-0Universidade Estadual Paulista (Unesp)Pinheiro, Luiza [UNESP]Brito, Carla Ivo [UNESP]Oliveira, Adilson de [UNESP]Martins, Patrícia Yoshida Faccioli [UNESP]Pereira, Valéria Cataneli [UNESP]Cunha, Maria de Lourdes Ribeiro de Souza da [UNESP]2015-12-07T15:32:51Z2015-12-07T15:32:51Z2015info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article3688-3699application/pdfhttp://dx.doi.org/10.3390/toxins7093688Toxins, v. 7, n. 9, p. 3688-3699, 2015.2072-6651http://hdl.handle.net/11449/13123010.3390/toxins7093688PMC4591658.pdf011564777231597326389954PMC4591658PubMedreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengToxins3.2730,955info:eu-repo/semantics/openAccess2023-10-14T06:05:47Zoai:repositorio.unesp.br:11449/131230Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:52:51.417568Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Staphylococcus epidermidis and Staphylococcus haemolyticus: molecular detection of cytotoxin and enterotoxin genes
title Staphylococcus epidermidis and Staphylococcus haemolyticus: molecular detection of cytotoxin and enterotoxin genes
spellingShingle Staphylococcus epidermidis and Staphylococcus haemolyticus: molecular detection of cytotoxin and enterotoxin genes
Pinheiro, Luiza [UNESP]
Staphylococcus epidermidis
Staphylococcus haemolyticus
Cytotoxins
Enterotoxins
title_short Staphylococcus epidermidis and Staphylococcus haemolyticus: molecular detection of cytotoxin and enterotoxin genes
title_full Staphylococcus epidermidis and Staphylococcus haemolyticus: molecular detection of cytotoxin and enterotoxin genes
title_fullStr Staphylococcus epidermidis and Staphylococcus haemolyticus: molecular detection of cytotoxin and enterotoxin genes
title_full_unstemmed Staphylococcus epidermidis and Staphylococcus haemolyticus: molecular detection of cytotoxin and enterotoxin genes
title_sort Staphylococcus epidermidis and Staphylococcus haemolyticus: molecular detection of cytotoxin and enterotoxin genes
author Pinheiro, Luiza [UNESP]
author_facet Pinheiro, Luiza [UNESP]
Brito, Carla Ivo [UNESP]
Oliveira, Adilson de [UNESP]
Martins, Patrícia Yoshida Faccioli [UNESP]
Pereira, Valéria Cataneli [UNESP]
Cunha, Maria de Lourdes Ribeiro de Souza da [UNESP]
author_role author
author2 Brito, Carla Ivo [UNESP]
Oliveira, Adilson de [UNESP]
Martins, Patrícia Yoshida Faccioli [UNESP]
Pereira, Valéria Cataneli [UNESP]
Cunha, Maria de Lourdes Ribeiro de Souza da [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Pinheiro, Luiza [UNESP]
Brito, Carla Ivo [UNESP]
Oliveira, Adilson de [UNESP]
Martins, Patrícia Yoshida Faccioli [UNESP]
Pereira, Valéria Cataneli [UNESP]
Cunha, Maria de Lourdes Ribeiro de Souza da [UNESP]
dc.subject.por.fl_str_mv Staphylococcus epidermidis
Staphylococcus haemolyticus
Cytotoxins
Enterotoxins
topic Staphylococcus epidermidis
Staphylococcus haemolyticus
Cytotoxins
Enterotoxins
description Although opportunistic pathogens, coagulase-negative staphylococci (CoNS), including Staphylococcus epidermidis and Staphylococcus haemolyticus, have long been regarded as avirulent organisms. The role of toxins in the development of infections caused by CoNS is still controversial. The objective of this study was to characterize the presence of enterotoxin and cytotoxin genes in S. epidermidis and S. haemolyticus isolates obtained from blood cultures. Cytotoxin genes were detected by PCR using novel species-specific primers. Among the 85 S. epidermidis and 84 S. haemolyticus isolates, 95.3% and 79.8%, respectively, carried at least one enterotoxin gene. The most frequent enterotoxin genes were sea (53.3%), seg (64.5%) and sei (67.5%). The seg gene was positively associated with S. epidermidis (p = 0.02), and this species was more toxigenic than S. haemolyticus. The hla/yidD gene was detected in 92.9% of S. epidermidis and the hla gene in 91.7% of S. haemolyticus isolates; hlb was detected in 92.9% of the S. epidermidis isolates and hld in 95.3%. Nosocomial Staphylococcus epidermidis and S. haemolyticus isolates exhibited a high toxigenic potential, mainly producing the non-classical enterotoxins seg and sei. The previously unreported detection of hla/yidD and hlb in S. epidermidis and S. haemolyticus using species-specific primers showed that these hemolysin genes differ between CoNS species and that they are highly frequent in blood culture isolates.
publishDate 2015
dc.date.none.fl_str_mv 2015-12-07T15:32:51Z
2015-12-07T15:32:51Z
2015
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3390/toxins7093688
Toxins, v. 7, n. 9, p. 3688-3699, 2015.
2072-6651
http://hdl.handle.net/11449/131230
10.3390/toxins7093688
PMC4591658.pdf
0115647772315973
26389954
PMC4591658
url http://dx.doi.org/10.3390/toxins7093688
http://hdl.handle.net/11449/131230
identifier_str_mv Toxins, v. 7, n. 9, p. 3688-3699, 2015.
2072-6651
10.3390/toxins7093688
PMC4591658.pdf
0115647772315973
26389954
PMC4591658
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Toxins
3.273
0,955
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 3688-3699
application/pdf
dc.source.none.fl_str_mv PubMed
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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