The urea transporter DUR3 is differentially regulated by abiotic and biotic stresses in coffee plants
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s12298-021-00930-6 http://hdl.handle.net/11449/207244 |
Resumo: | The high costs of N fertilizers in the coffee production emphasizes the need to optimize fertilization practices and improve nitrogen use efficiency. Urea is widespread in nature, characterizing itself as a significant source of nitrogen for the growth and development of several organisms. Thus, the characterization of genes involved in urea transport in coffee plants is an important research topic for the sustainable production of this valuable cash crop. In the current study, we evaluated the expression of the DUR3 gene under abiotic and biotic stresses in coffee plants. Here, we show that the expression of a high-affinity urea transporter gene (CaDUR3) was up-regulated by N starvation in leaves and roots of two out of three C. arabica cultivars examined. Moreover, the CaDUR3 gene was differentially expressed in coffee plants under different abiotic and biotic stresses. In plants of cv. IAPAR59, CaDUR3 showed an increased expression in leaves after exposure to water deficit and heat stress, while it was downregulated in plants under salinity. Upon infection with H. vastatrix (coffee rust), the CaDUR3 was markedly up-regulated at the beginning of the infection process in the disease susceptible Catuaí Vermelho 99 in comparison with the resistant cultivar. These results indicate that besides urea acquisition and N-remobilization, CaDUR3 gene may be closely involved in the response to various stresses. |
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The urea transporter DUR3 is differentially regulated by abiotic and biotic stresses in coffee plantsCoffea sspGene expressionNitrogen deficiencyUrea transporterThe high costs of N fertilizers in the coffee production emphasizes the need to optimize fertilization practices and improve nitrogen use efficiency. Urea is widespread in nature, characterizing itself as a significant source of nitrogen for the growth and development of several organisms. Thus, the characterization of genes involved in urea transport in coffee plants is an important research topic for the sustainable production of this valuable cash crop. In the current study, we evaluated the expression of the DUR3 gene under abiotic and biotic stresses in coffee plants. Here, we show that the expression of a high-affinity urea transporter gene (CaDUR3) was up-regulated by N starvation in leaves and roots of two out of three C. arabica cultivars examined. Moreover, the CaDUR3 gene was differentially expressed in coffee plants under different abiotic and biotic stresses. In plants of cv. IAPAR59, CaDUR3 showed an increased expression in leaves after exposure to water deficit and heat stress, while it was downregulated in plants under salinity. Upon infection with H. vastatrix (coffee rust), the CaDUR3 was markedly up-regulated at the beginning of the infection process in the disease susceptible Catuaí Vermelho 99 in comparison with the resistant cultivar. These results indicate that besides urea acquisition and N-remobilization, CaDUR3 gene may be closely involved in the response to various stresses.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Laboratório de Biotecnologia Vegetal Instituto Agronômico Do Paraná (IAPAR) LondrinaUniversidade Do Oeste Paulista (UNOESTE), Rodovia Raposo Tavares, Km 572Empresa Brasileira de Pesquisa Agropecuária (Embrapa Café)Departamento de Botânica Instituto de Biociências de Rio Claro Universidade Estadual Paulista (UNESP), Avenida 24-A, 1515Departamento de Botânica Instituto de Biociências de Rio Claro Universidade Estadual Paulista (UNESP), Avenida 24-A, 1515LondrinaUniversidade Do Oeste Paulista (UNOESTE)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Universidade Estadual Paulista (Unesp)dos Santos, Tiago BeneditoBaba, Viviane Y.Vieira, Luiz Gonzaga EstevesPereira, Luiz Filipe ProtasioDomingues, Douglas Silva [UNESP]2021-06-25T10:51:47Z2021-06-25T10:51:47Z2021-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article203-212http://dx.doi.org/10.1007/s12298-021-00930-6Physiology and Molecular Biology of Plants, v. 27, n. 2, p. 203-212, 2021.0974-04300971-5894http://hdl.handle.net/11449/20724410.1007/s12298-021-00930-62-s2.0-85100484253Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPhysiology and Molecular Biology of Plantsinfo:eu-repo/semantics/openAccess2021-10-23T16:37:22Zoai:repositorio.unesp.br:11449/207244Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T16:37:22Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The urea transporter DUR3 is differentially regulated by abiotic and biotic stresses in coffee plants |
title |
The urea transporter DUR3 is differentially regulated by abiotic and biotic stresses in coffee plants |
spellingShingle |
The urea transporter DUR3 is differentially regulated by abiotic and biotic stresses in coffee plants dos Santos, Tiago Benedito Coffea ssp Gene expression Nitrogen deficiency Urea transporter |
title_short |
The urea transporter DUR3 is differentially regulated by abiotic and biotic stresses in coffee plants |
title_full |
The urea transporter DUR3 is differentially regulated by abiotic and biotic stresses in coffee plants |
title_fullStr |
The urea transporter DUR3 is differentially regulated by abiotic and biotic stresses in coffee plants |
title_full_unstemmed |
The urea transporter DUR3 is differentially regulated by abiotic and biotic stresses in coffee plants |
title_sort |
The urea transporter DUR3 is differentially regulated by abiotic and biotic stresses in coffee plants |
author |
dos Santos, Tiago Benedito |
author_facet |
dos Santos, Tiago Benedito Baba, Viviane Y. Vieira, Luiz Gonzaga Esteves Pereira, Luiz Filipe Protasio Domingues, Douglas Silva [UNESP] |
author_role |
author |
author2 |
Baba, Viviane Y. Vieira, Luiz Gonzaga Esteves Pereira, Luiz Filipe Protasio Domingues, Douglas Silva [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Londrina Universidade Do Oeste Paulista (UNOESTE) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
dos Santos, Tiago Benedito Baba, Viviane Y. Vieira, Luiz Gonzaga Esteves Pereira, Luiz Filipe Protasio Domingues, Douglas Silva [UNESP] |
dc.subject.por.fl_str_mv |
Coffea ssp Gene expression Nitrogen deficiency Urea transporter |
topic |
Coffea ssp Gene expression Nitrogen deficiency Urea transporter |
description |
The high costs of N fertilizers in the coffee production emphasizes the need to optimize fertilization practices and improve nitrogen use efficiency. Urea is widespread in nature, characterizing itself as a significant source of nitrogen for the growth and development of several organisms. Thus, the characterization of genes involved in urea transport in coffee plants is an important research topic for the sustainable production of this valuable cash crop. In the current study, we evaluated the expression of the DUR3 gene under abiotic and biotic stresses in coffee plants. Here, we show that the expression of a high-affinity urea transporter gene (CaDUR3) was up-regulated by N starvation in leaves and roots of two out of three C. arabica cultivars examined. Moreover, the CaDUR3 gene was differentially expressed in coffee plants under different abiotic and biotic stresses. In plants of cv. IAPAR59, CaDUR3 showed an increased expression in leaves after exposure to water deficit and heat stress, while it was downregulated in plants under salinity. Upon infection with H. vastatrix (coffee rust), the CaDUR3 was markedly up-regulated at the beginning of the infection process in the disease susceptible Catuaí Vermelho 99 in comparison with the resistant cultivar. These results indicate that besides urea acquisition and N-remobilization, CaDUR3 gene may be closely involved in the response to various stresses. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:51:47Z 2021-06-25T10:51:47Z 2021-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s12298-021-00930-6 Physiology and Molecular Biology of Plants, v. 27, n. 2, p. 203-212, 2021. 0974-0430 0971-5894 http://hdl.handle.net/11449/207244 10.1007/s12298-021-00930-6 2-s2.0-85100484253 |
url |
http://dx.doi.org/10.1007/s12298-021-00930-6 http://hdl.handle.net/11449/207244 |
identifier_str_mv |
Physiology and Molecular Biology of Plants, v. 27, n. 2, p. 203-212, 2021. 0974-0430 0971-5894 10.1007/s12298-021-00930-6 2-s2.0-85100484253 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Physiology and Molecular Biology of Plants |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
203-212 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797790124927352832 |