Lipases and lipid droplet-associated protein expression in subcutaneous white adipose tissue of cachectic patients with cancer
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/s12944-017-0547-x http://hdl.handle.net/11449/170052 |
Resumo: | Background: Cancer cachexia is a multifactorial metabolic syndrome characterized by marked loss of adipose tissue and skeletal muscle. Fat loss from adipose tissue in cancer cachexia is partly the result of increased lipolysis. Despite the growing amount of studies focused on elucidating the mechanisms through which lipolysis-related proteins regulate the lipolytic process, there are scarce data concerning that profile in the adipose tissue of cancer cachectic patients. Considering its fundamental importance, it was our main purpose to characterize the expression of the lipolysis-related proteins in the white adipose tissue of cachectic cancer patients. Methods: Patients from the University Hospital were divided into three groups: control, cancer cachexia (CC), and weight-stable cancer patients (WSC). To gain greater insight into adipose tissue wasting during cancer cachexia progression, we have also analyzed an experimental model of cachexia (Walker 256 carcinosarcoma). Animals were divided into: control, intermediate cachexia (IC) and terminal cachexia (TC). Subcutaneous white adipose tissue of patients and epidydimal white adipose tissue of animals were investigated regarding molecular aspects by determining the protein content and gene expression of hormone-sensitive lipase (HSL), adipose triglyceride lipase (ATGL), comparative gene identification-58 (CGI-58), perilipin 1, leptin, adiponectin, visfatin, and tumour necrosis factor alpha (TNF-alpha). Results: We found augmented lipolysis in CC associated with increased HSL expression, as well as upregulation of ATGL expression and reduction in perilipin 1 content. In IC, there was an imbalance in the secretion of pro- and anti-inflammatory factors. The alterations at the end-stage of cachexia were even more profound, and there was a reduction in the expression of almost all proteins analyzed in the animals. Conclusions: Our findings show that cachexia induces important morphological, molecular, and humoral alterations in the white adipose tissue, which are specific to the stage of the syndrome. |
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Lipases and lipid droplet-associated protein expression in subcutaneous white adipose tissue of cachectic patients with cancerBackground: Cancer cachexia is a multifactorial metabolic syndrome characterized by marked loss of adipose tissue and skeletal muscle. Fat loss from adipose tissue in cancer cachexia is partly the result of increased lipolysis. Despite the growing amount of studies focused on elucidating the mechanisms through which lipolysis-related proteins regulate the lipolytic process, there are scarce data concerning that profile in the adipose tissue of cancer cachectic patients. Considering its fundamental importance, it was our main purpose to characterize the expression of the lipolysis-related proteins in the white adipose tissue of cachectic cancer patients. Methods: Patients from the University Hospital were divided into three groups: control, cancer cachexia (CC), and weight-stable cancer patients (WSC). To gain greater insight into adipose tissue wasting during cancer cachexia progression, we have also analyzed an experimental model of cachexia (Walker 256 carcinosarcoma). Animals were divided into: control, intermediate cachexia (IC) and terminal cachexia (TC). Subcutaneous white adipose tissue of patients and epidydimal white adipose tissue of animals were investigated regarding molecular aspects by determining the protein content and gene expression of hormone-sensitive lipase (HSL), adipose triglyceride lipase (ATGL), comparative gene identification-58 (CGI-58), perilipin 1, leptin, adiponectin, visfatin, and tumour necrosis factor alpha (TNF-alpha). Results: We found augmented lipolysis in CC associated with increased HSL expression, as well as upregulation of ATGL expression and reduction in perilipin 1 content. In IC, there was an imbalance in the secretion of pro- and anti-inflammatory factors. The alterations at the end-stage of cachexia were even more profound, and there was a reduction in the expression of almost all proteins analyzed in the animals. Conclusions: Our findings show that cachexia induces important morphological, molecular, and humoral alterations in the white adipose tissue, which are specific to the stage of the syndrome.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Cancer Metabolism Research Group Institute of Biomedical Sciences Department of Surgery Faculdade de Medicina University of São PauloExercise and Immunometabolism Research Group Department of Physical Education São Paulo State University (UNESP)Departamento de Fisiologia Universidade Federal de São Paulo UNIFESPDepartment of Clinical Surgery University Hospital University of São PauloLaboratory of Adipose Tissue Biology Center for Integrated Biotechnology University of Mogi das CruzesInstitute of Biomedical Sciences University of São Paulo, Av. Prof. Lineu Prestes, 1524Exercise and Immunometabolism Research Group Department of Physical Education São Paulo State University (UNESP)Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Universidade Federal de São Paulo (UNIFESP)University of Mogi das CruzesSilvério, RenataLira, Fábio S. [UNESP]Oyama, Lila M.Oller Do Nascimento, Cláudia M.Otoch, José P.Alcântara, Paulo S. M.Batista, Miguel L.Seelaender, Marília2018-12-11T16:48:54Z2018-12-11T16:48:54Z2017-08-22info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1186/s12944-017-0547-xLipids in Health and Disease, v. 16, n. 1, 2017.1476-511Xhttp://hdl.handle.net/11449/17005210.1186/s12944-017-0547-x2-s2.0-850279692132-s2.0-85027969213.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLipids in Health and Diseaseinfo:eu-repo/semantics/openAccess2023-11-25T06:12:02Zoai:repositorio.unesp.br:11449/170052Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-25T06:12:02Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Lipases and lipid droplet-associated protein expression in subcutaneous white adipose tissue of cachectic patients with cancer |
title |
Lipases and lipid droplet-associated protein expression in subcutaneous white adipose tissue of cachectic patients with cancer |
spellingShingle |
Lipases and lipid droplet-associated protein expression in subcutaneous white adipose tissue of cachectic patients with cancer Silvério, Renata |
title_short |
Lipases and lipid droplet-associated protein expression in subcutaneous white adipose tissue of cachectic patients with cancer |
title_full |
Lipases and lipid droplet-associated protein expression in subcutaneous white adipose tissue of cachectic patients with cancer |
title_fullStr |
Lipases and lipid droplet-associated protein expression in subcutaneous white adipose tissue of cachectic patients with cancer |
title_full_unstemmed |
Lipases and lipid droplet-associated protein expression in subcutaneous white adipose tissue of cachectic patients with cancer |
title_sort |
Lipases and lipid droplet-associated protein expression in subcutaneous white adipose tissue of cachectic patients with cancer |
author |
Silvério, Renata |
author_facet |
Silvério, Renata Lira, Fábio S. [UNESP] Oyama, Lila M. Oller Do Nascimento, Cláudia M. Otoch, José P. Alcântara, Paulo S. M. Batista, Miguel L. Seelaender, Marília |
author_role |
author |
author2 |
Lira, Fábio S. [UNESP] Oyama, Lila M. Oller Do Nascimento, Cláudia M. Otoch, José P. Alcântara, Paulo S. M. Batista, Miguel L. Seelaender, Marília |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) Universidade Federal de São Paulo (UNIFESP) University of Mogi das Cruzes |
dc.contributor.author.fl_str_mv |
Silvério, Renata Lira, Fábio S. [UNESP] Oyama, Lila M. Oller Do Nascimento, Cláudia M. Otoch, José P. Alcântara, Paulo S. M. Batista, Miguel L. Seelaender, Marília |
description |
Background: Cancer cachexia is a multifactorial metabolic syndrome characterized by marked loss of adipose tissue and skeletal muscle. Fat loss from adipose tissue in cancer cachexia is partly the result of increased lipolysis. Despite the growing amount of studies focused on elucidating the mechanisms through which lipolysis-related proteins regulate the lipolytic process, there are scarce data concerning that profile in the adipose tissue of cancer cachectic patients. Considering its fundamental importance, it was our main purpose to characterize the expression of the lipolysis-related proteins in the white adipose tissue of cachectic cancer patients. Methods: Patients from the University Hospital were divided into three groups: control, cancer cachexia (CC), and weight-stable cancer patients (WSC). To gain greater insight into adipose tissue wasting during cancer cachexia progression, we have also analyzed an experimental model of cachexia (Walker 256 carcinosarcoma). Animals were divided into: control, intermediate cachexia (IC) and terminal cachexia (TC). Subcutaneous white adipose tissue of patients and epidydimal white adipose tissue of animals were investigated regarding molecular aspects by determining the protein content and gene expression of hormone-sensitive lipase (HSL), adipose triglyceride lipase (ATGL), comparative gene identification-58 (CGI-58), perilipin 1, leptin, adiponectin, visfatin, and tumour necrosis factor alpha (TNF-alpha). Results: We found augmented lipolysis in CC associated with increased HSL expression, as well as upregulation of ATGL expression and reduction in perilipin 1 content. In IC, there was an imbalance in the secretion of pro- and anti-inflammatory factors. The alterations at the end-stage of cachexia were even more profound, and there was a reduction in the expression of almost all proteins analyzed in the animals. Conclusions: Our findings show that cachexia induces important morphological, molecular, and humoral alterations in the white adipose tissue, which are specific to the stage of the syndrome. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-08-22 2018-12-11T16:48:54Z 2018-12-11T16:48:54Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/s12944-017-0547-x Lipids in Health and Disease, v. 16, n. 1, 2017. 1476-511X http://hdl.handle.net/11449/170052 10.1186/s12944-017-0547-x 2-s2.0-85027969213 2-s2.0-85027969213.pdf |
url |
http://dx.doi.org/10.1186/s12944-017-0547-x http://hdl.handle.net/11449/170052 |
identifier_str_mv |
Lipids in Health and Disease, v. 16, n. 1, 2017. 1476-511X 10.1186/s12944-017-0547-x 2-s2.0-85027969213 2-s2.0-85027969213.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Lipids in Health and Disease |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1803046645362851840 |