X-ray fluorescence spectroscopy (XRF) applied to plant science: Challenges towards: In vivo analysis of plants
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1039/c9mt00237e http://hdl.handle.net/11449/198584 |
Resumo: | X-ray fluorescence spectroscopy (XRF) is an analytical tool used to determine the elemental composition in a myriad of sample matrices. Due to the XRF non-destructive feature, this technique may allow time-resolved plant tissue analyses under in vivo conditions, and additionally, the combination with other non-destructive techniques. In this study, we employed handheld and benchtop XRF to evaluate the elemental distribution changes in living plant tissues exposed to X-rays, as well as real-time uptake kinetics of Zn(aq) and Mn(aq) in soybean (Glycine max (L.) Merrill) stem and leaves, for 48 hours, combined with transpiration rate assessment on leaves by an infrared gas analyzer (IRGA). We found higher Zn content than Mn in stems. The latter micronutrient, in turn, presented higher concentration in leaf veins. Besides, both micronutrients were more concentrated in the first trifolium (i.e., youngest leaf) of soybean plants. Moreover, the transpiration rate was more influenced by circadian cycles than Zn and Mn uptake. Thus, XRF represents a convenient tool for in vivo nutritional studies in plants, and it can be coupled successfully to other analytical techniques. |
id |
UNSP_68624d7c20cca391270987ff99d1aa8e |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/198584 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
X-ray fluorescence spectroscopy (XRF) applied to plant science: Challenges towards: In vivo analysis of plantsX-ray fluorescence spectroscopy (XRF) is an analytical tool used to determine the elemental composition in a myriad of sample matrices. Due to the XRF non-destructive feature, this technique may allow time-resolved plant tissue analyses under in vivo conditions, and additionally, the combination with other non-destructive techniques. In this study, we employed handheld and benchtop XRF to evaluate the elemental distribution changes in living plant tissues exposed to X-rays, as well as real-time uptake kinetics of Zn(aq) and Mn(aq) in soybean (Glycine max (L.) Merrill) stem and leaves, for 48 hours, combined with transpiration rate assessment on leaves by an infrared gas analyzer (IRGA). We found higher Zn content than Mn in stems. The latter micronutrient, in turn, presented higher concentration in leaf veins. Besides, both micronutrients were more concentrated in the first trifolium (i.e., youngest leaf) of soybean plants. Moreover, the transpiration rate was more influenced by circadian cycles than Zn and Mn uptake. Thus, XRF represents a convenient tool for in vivo nutritional studies in plants, and it can be coupled successfully to other analytical techniques.Center for Nuclear Energy in Agriculture (CENA) University of Saõ Paulo (USP), Avenida Centenário, 303Saõ Paulo State University-UNESP, Rua Domingos da Costa Lopes 780Saõ Paulo State University-UNESP, Rua Domingos da Costa Lopes 780Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Montanha, Gabriel SgarbieroRodrigues, Eduardo SantosMarques, Joaõ Paulo RodriguesDe Almeida, EduardoDos Reis, André Rodrigues [UNESP]Pereira De Carvalho, Hudson Wallace2020-12-12T01:16:52Z2020-12-12T01:16:52Z2020-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article183-192http://dx.doi.org/10.1039/c9mt00237eMetallomics, v. 12, n. 2, p. 183-192, 2020.1756-591X1756-5901http://hdl.handle.net/11449/19858410.1039/c9mt00237e2-s2.0-85080898194Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMetallomicsinfo:eu-repo/semantics/openAccess2021-10-22T17:11:25Zoai:repositorio.unesp.br:11449/198584Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:21:53.480410Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
X-ray fluorescence spectroscopy (XRF) applied to plant science: Challenges towards: In vivo analysis of plants |
title |
X-ray fluorescence spectroscopy (XRF) applied to plant science: Challenges towards: In vivo analysis of plants |
spellingShingle |
X-ray fluorescence spectroscopy (XRF) applied to plant science: Challenges towards: In vivo analysis of plants Montanha, Gabriel Sgarbiero |
title_short |
X-ray fluorescence spectroscopy (XRF) applied to plant science: Challenges towards: In vivo analysis of plants |
title_full |
X-ray fluorescence spectroscopy (XRF) applied to plant science: Challenges towards: In vivo analysis of plants |
title_fullStr |
X-ray fluorescence spectroscopy (XRF) applied to plant science: Challenges towards: In vivo analysis of plants |
title_full_unstemmed |
X-ray fluorescence spectroscopy (XRF) applied to plant science: Challenges towards: In vivo analysis of plants |
title_sort |
X-ray fluorescence spectroscopy (XRF) applied to plant science: Challenges towards: In vivo analysis of plants |
author |
Montanha, Gabriel Sgarbiero |
author_facet |
Montanha, Gabriel Sgarbiero Rodrigues, Eduardo Santos Marques, Joaõ Paulo Rodrigues De Almeida, Eduardo Dos Reis, André Rodrigues [UNESP] Pereira De Carvalho, Hudson Wallace |
author_role |
author |
author2 |
Rodrigues, Eduardo Santos Marques, Joaõ Paulo Rodrigues De Almeida, Eduardo Dos Reis, André Rodrigues [UNESP] Pereira De Carvalho, Hudson Wallace |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Montanha, Gabriel Sgarbiero Rodrigues, Eduardo Santos Marques, Joaõ Paulo Rodrigues De Almeida, Eduardo Dos Reis, André Rodrigues [UNESP] Pereira De Carvalho, Hudson Wallace |
description |
X-ray fluorescence spectroscopy (XRF) is an analytical tool used to determine the elemental composition in a myriad of sample matrices. Due to the XRF non-destructive feature, this technique may allow time-resolved plant tissue analyses under in vivo conditions, and additionally, the combination with other non-destructive techniques. In this study, we employed handheld and benchtop XRF to evaluate the elemental distribution changes in living plant tissues exposed to X-rays, as well as real-time uptake kinetics of Zn(aq) and Mn(aq) in soybean (Glycine max (L.) Merrill) stem and leaves, for 48 hours, combined with transpiration rate assessment on leaves by an infrared gas analyzer (IRGA). We found higher Zn content than Mn in stems. The latter micronutrient, in turn, presented higher concentration in leaf veins. Besides, both micronutrients were more concentrated in the first trifolium (i.e., youngest leaf) of soybean plants. Moreover, the transpiration rate was more influenced by circadian cycles than Zn and Mn uptake. Thus, XRF represents a convenient tool for in vivo nutritional studies in plants, and it can be coupled successfully to other analytical techniques. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T01:16:52Z 2020-12-12T01:16:52Z 2020-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1039/c9mt00237e Metallomics, v. 12, n. 2, p. 183-192, 2020. 1756-591X 1756-5901 http://hdl.handle.net/11449/198584 10.1039/c9mt00237e 2-s2.0-85080898194 |
url |
http://dx.doi.org/10.1039/c9mt00237e http://hdl.handle.net/11449/198584 |
identifier_str_mv |
Metallomics, v. 12, n. 2, p. 183-192, 2020. 1756-591X 1756-5901 10.1039/c9mt00237e 2-s2.0-85080898194 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Metallomics |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
183-192 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128352903495680 |