Low variation in ribosomal DNA and internal transcribed spacers of the symbiotic fungi of leaf-cutting ants (Attini: Formicidae)
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0100-879X2004001000004 http://hdl.handle.net/11449/757 |
Resumo: | Leaf-cutting ants of the genera Atta and Acromyrmex (tribe Attini) are symbiotic with basidiomycete fungi of the genus Leucoagaricus (tribe Leucocoprineae), which they cultivate on vegetable matter inside their nests. We determined the variation of the 28S, 18S, and 5.8S ribosomal DNA (rDNA) gene loci and the rapidly evolving internal transcribed spacers 1 and 2 (ITS1 and ITS2) of 15 sympatric and allopatric fungi associated with colonies of 11 species of leafcutter ants living up to 2,600 km apart in Brazil. We found that the fungal rDNA and ITS sequences from different species of ants were identical (or nearly identical) to each other, whereas 10 GenBank Leucoagaricus species showed higher ITS variation. Our findings suggest that Atta and Acromyrmex leafcutters living in geographic sites that are very distant from each other cultivate a single fungal species made up of closely related lineages of Leucoagaricus gongylophorus. We discuss the strikingly high similarity in the ITS1 and ITS2 regions of the Atta and Acromyrmex symbiotic L. gongylophorus studied by us, in contrast to the lower similarity displayed by their non-symbiotic counterparts. We suggest that the similarity of our L. gongylophorus isolates is an indication of the recent association of the fungus with these ants, and propose that both the intense lateral transmission of fungal material within leafcutter nests and the selection of more adapted fungal strains are involved in the homogenization of the symbiotic fungal stock. |
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Repositório Institucional da UNESP |
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Low variation in ribosomal DNA and internal transcribed spacers of the symbiotic fungi of leaf-cutting ants (Attini: Formicidae)AttaAcromyrmexInternal transcribed spacerLeaf-cutting antsLeucoagaricusLeucoagaricus gongylophorusLeaf-cutting ants of the genera Atta and Acromyrmex (tribe Attini) are symbiotic with basidiomycete fungi of the genus Leucoagaricus (tribe Leucocoprineae), which they cultivate on vegetable matter inside their nests. We determined the variation of the 28S, 18S, and 5.8S ribosomal DNA (rDNA) gene loci and the rapidly evolving internal transcribed spacers 1 and 2 (ITS1 and ITS2) of 15 sympatric and allopatric fungi associated with colonies of 11 species of leafcutter ants living up to 2,600 km apart in Brazil. We found that the fungal rDNA and ITS sequences from different species of ants were identical (or nearly identical) to each other, whereas 10 GenBank Leucoagaricus species showed higher ITS variation. Our findings suggest that Atta and Acromyrmex leafcutters living in geographic sites that are very distant from each other cultivate a single fungal species made up of closely related lineages of Leucoagaricus gongylophorus. We discuss the strikingly high similarity in the ITS1 and ITS2 regions of the Atta and Acromyrmex symbiotic L. gongylophorus studied by us, in contrast to the lower similarity displayed by their non-symbiotic counterparts. We suggest that the similarity of our L. gongylophorus isolates is an indication of the recent association of the fungus with these ants, and propose that both the intense lateral transmission of fungal material within leafcutter nests and the selection of more adapted fungal strains are involved in the homogenization of the symbiotic fungal stock.Universidade Estadual Paulista Centro de Estudos de Insetos SociaisUniversidade Estadual Paulista Departamento de Bioquímica e MicrobiologiaUniversidade Estadual Paulista Instituto de Biociências Depatamento de BiologiaUniversidade Estadual Paulista Departamento de FonoaudiologiaMarine Biological Laboratory Josephine Bay Paul Center for Comparative Molecular Biology and EvolutionUniversidade Estadual Paulista Centro de Estudos de Insetos SociaisUniversidade Estadual Paulista Departamento de Bioquímica e MicrobiologiaUniversidade Estadual Paulista Instituto de Biociências Depatamento de BiologiaUniversidade Estadual Paulista Departamento de FonoaudiologiaAssociação Brasileira de Divulgação Científica (ABRADIC)Universidade Estadual Paulista (Unesp)Marine Biological Laboratory Josephine Bay Paul Center for Comparative Molecular Biology and EvolutionSilva-Pinhati, A. C. O. [UNESP]Bacci Junior, Mauricio [UNESP]Hinkle, G.Sogin, M.L.Pagnocca, Fernando Carlos [UNESP]Martins, V. G. [UNESP]Bueno, O. C. [UNESP]Hebling, Maria José Aparecida [UNESP]2014-05-20T13:12:50Z2014-05-20T13:12:50Z2004-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1463-1472application/pdfhttp://dx.doi.org/10.1590/S0100-879X2004001000004Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 37, n. 10, p. 1463-1472, 2004.0100-879Xhttp://hdl.handle.net/11449/75710.1590/S0100-879X2004001000004S0100-879X2004001000004S0100-879X2004001000004.pdf3776345573864268SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Medical and Biological Research1.492info:eu-repo/semantics/openAccess2024-04-11T14:57:28Zoai:repositorio.unesp.br:11449/757Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-11T14:57:28Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Low variation in ribosomal DNA and internal transcribed spacers of the symbiotic fungi of leaf-cutting ants (Attini: Formicidae) |
title |
Low variation in ribosomal DNA and internal transcribed spacers of the symbiotic fungi of leaf-cutting ants (Attini: Formicidae) |
spellingShingle |
Low variation in ribosomal DNA and internal transcribed spacers of the symbiotic fungi of leaf-cutting ants (Attini: Formicidae) Silva-Pinhati, A. C. O. [UNESP] Atta Acromyrmex Internal transcribed spacer Leaf-cutting ants Leucoagaricus Leucoagaricus gongylophorus |
title_short |
Low variation in ribosomal DNA and internal transcribed spacers of the symbiotic fungi of leaf-cutting ants (Attini: Formicidae) |
title_full |
Low variation in ribosomal DNA and internal transcribed spacers of the symbiotic fungi of leaf-cutting ants (Attini: Formicidae) |
title_fullStr |
Low variation in ribosomal DNA and internal transcribed spacers of the symbiotic fungi of leaf-cutting ants (Attini: Formicidae) |
title_full_unstemmed |
Low variation in ribosomal DNA and internal transcribed spacers of the symbiotic fungi of leaf-cutting ants (Attini: Formicidae) |
title_sort |
Low variation in ribosomal DNA and internal transcribed spacers of the symbiotic fungi of leaf-cutting ants (Attini: Formicidae) |
author |
Silva-Pinhati, A. C. O. [UNESP] |
author_facet |
Silva-Pinhati, A. C. O. [UNESP] Bacci Junior, Mauricio [UNESP] Hinkle, G. Sogin, M.L. Pagnocca, Fernando Carlos [UNESP] Martins, V. G. [UNESP] Bueno, O. C. [UNESP] Hebling, Maria José Aparecida [UNESP] |
author_role |
author |
author2 |
Bacci Junior, Mauricio [UNESP] Hinkle, G. Sogin, M.L. Pagnocca, Fernando Carlos [UNESP] Martins, V. G. [UNESP] Bueno, O. C. [UNESP] Hebling, Maria José Aparecida [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Marine Biological Laboratory Josephine Bay Paul Center for Comparative Molecular Biology and Evolution |
dc.contributor.author.fl_str_mv |
Silva-Pinhati, A. C. O. [UNESP] Bacci Junior, Mauricio [UNESP] Hinkle, G. Sogin, M.L. Pagnocca, Fernando Carlos [UNESP] Martins, V. G. [UNESP] Bueno, O. C. [UNESP] Hebling, Maria José Aparecida [UNESP] |
dc.subject.por.fl_str_mv |
Atta Acromyrmex Internal transcribed spacer Leaf-cutting ants Leucoagaricus Leucoagaricus gongylophorus |
topic |
Atta Acromyrmex Internal transcribed spacer Leaf-cutting ants Leucoagaricus Leucoagaricus gongylophorus |
description |
Leaf-cutting ants of the genera Atta and Acromyrmex (tribe Attini) are symbiotic with basidiomycete fungi of the genus Leucoagaricus (tribe Leucocoprineae), which they cultivate on vegetable matter inside their nests. We determined the variation of the 28S, 18S, and 5.8S ribosomal DNA (rDNA) gene loci and the rapidly evolving internal transcribed spacers 1 and 2 (ITS1 and ITS2) of 15 sympatric and allopatric fungi associated with colonies of 11 species of leafcutter ants living up to 2,600 km apart in Brazil. We found that the fungal rDNA and ITS sequences from different species of ants were identical (or nearly identical) to each other, whereas 10 GenBank Leucoagaricus species showed higher ITS variation. Our findings suggest that Atta and Acromyrmex leafcutters living in geographic sites that are very distant from each other cultivate a single fungal species made up of closely related lineages of Leucoagaricus gongylophorus. We discuss the strikingly high similarity in the ITS1 and ITS2 regions of the Atta and Acromyrmex symbiotic L. gongylophorus studied by us, in contrast to the lower similarity displayed by their non-symbiotic counterparts. We suggest that the similarity of our L. gongylophorus isolates is an indication of the recent association of the fungus with these ants, and propose that both the intense lateral transmission of fungal material within leafcutter nests and the selection of more adapted fungal strains are involved in the homogenization of the symbiotic fungal stock. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-10-01 2014-05-20T13:12:50Z 2014-05-20T13:12:50Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0100-879X2004001000004 Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 37, n. 10, p. 1463-1472, 2004. 0100-879X http://hdl.handle.net/11449/757 10.1590/S0100-879X2004001000004 S0100-879X2004001000004 S0100-879X2004001000004.pdf 3776345573864268 |
url |
http://dx.doi.org/10.1590/S0100-879X2004001000004 http://hdl.handle.net/11449/757 |
identifier_str_mv |
Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 37, n. 10, p. 1463-1472, 2004. 0100-879X 10.1590/S0100-879X2004001000004 S0100-879X2004001000004 S0100-879X2004001000004.pdf 3776345573864268 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Medical and Biological Research 1.492 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1463-1472 application/pdf |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica (ABRADIC) |
publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica (ABRADIC) |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965478533201920 |