Cytogenetic markers as diagnoses in the identification of the hybrid between Piauçu (Leporinus macrocephalus) and Piapara (Leporinus elongatus)
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1415-47572008000200005 http://hdl.handle.net/11449/8187 |
Resumo: | The genetic monitoring of interspecific hybrids involves the application of methodologies able to provide an easy and indubitable genetic characterization of both parental and hybrid individuals. In the present work, cytogenetic techniques were used to identify a hybrid lineage of Piaupara in order to caracterize them in relation to the parental species, Leporinus macrocephalus (piauçu) and L. elongatus (piapara). The cytogenetic analysis revealed that L. macrocephalus presented 2n = 54 chromosomes and a nucleolar organizer regions (NOR) at the telomere of the long arm of the submetacentric chromosome pair 2. Analysis of constitutive heterochromatin (C-banding) revealed a conspicuous block at the pericentromeric region on the long arm of a submetacentric chromosome pair. L. elongatus presented the same diploid number, 2n = 54, and a karyotypic formula similar to that of L. macrocephalus. The NORs were also at the telomere of the long arm of the submetacentric pair 2, which was morphologically different from that of L. macrocephalus. Heterochromatic blocks were observed at both telomeres of a submetacentric chromosome pair. The hybrid Piaupara presented the same diploid number (2n = 54) and karyotypic formula as the parental species and there were no visible differences between parental and hybrid individuals. Differently from the Giemsa staining, NOR- and C-banding analysis showed marked differences which allowed the identification of the hybrids by the different morphology and/or size of the chromosomes carrying the NORs and patterns of heterochromatin distribution in their chromosomes. Such genetic studies are important for fish culture since they can provide tools for monitoring natural and artificial hybridization. They are also useful in biological conservation programmes and in the proper management of natural and reared fish stocks. |
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Cytogenetic markers as diagnoses in the identification of the hybrid between Piauçu (Leporinus macrocephalus) and Piapara (Leporinus elongatus)interspecific hybridfish culturefish cytogeneticsThe genetic monitoring of interspecific hybrids involves the application of methodologies able to provide an easy and indubitable genetic characterization of both parental and hybrid individuals. In the present work, cytogenetic techniques were used to identify a hybrid lineage of Piaupara in order to caracterize them in relation to the parental species, Leporinus macrocephalus (piauçu) and L. elongatus (piapara). The cytogenetic analysis revealed that L. macrocephalus presented 2n = 54 chromosomes and a nucleolar organizer regions (NOR) at the telomere of the long arm of the submetacentric chromosome pair 2. Analysis of constitutive heterochromatin (C-banding) revealed a conspicuous block at the pericentromeric region on the long arm of a submetacentric chromosome pair. L. elongatus presented the same diploid number, 2n = 54, and a karyotypic formula similar to that of L. macrocephalus. The NORs were also at the telomere of the long arm of the submetacentric pair 2, which was morphologically different from that of L. macrocephalus. Heterochromatic blocks were observed at both telomeres of a submetacentric chromosome pair. The hybrid Piaupara presented the same diploid number (2n = 54) and karyotypic formula as the parental species and there were no visible differences between parental and hybrid individuals. Differently from the Giemsa staining, NOR- and C-banding analysis showed marked differences which allowed the identification of the hybrids by the different morphology and/or size of the chromosomes carrying the NORs and patterns of heterochromatin distribution in their chromosomes. Such genetic studies are important for fish culture since they can provide tools for monitoring natural and artificial hybridization. They are also useful in biological conservation programmes and in the proper management of natural and reared fish stocks.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Estadual Paulista Faculdade de Ciências Departamento de Ciências BiológicasUniversidade Estadual de Campinas Instituto de Biologia Departamento de ParasitologiaUniversidade Estadual Paulista Instituto de Biociências Departamento de MorfologiaInstituto Brasileiro do Meio Ambiente e dos Recursos Naturais Renováveis Centro de Pesquisa e Gestão de Recursos Pesqueiros ContinentaisUniversidade Estadual Paulista Faculdade de Ciências Departamento de Ciências BiológicasUniversidade Estadual Paulista Instituto de Biociências Departamento de MorfologiaSociedade Brasileira de GenéticaUniversidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Instituto Brasileiro do Meio Ambiente e dos Recursos Naturais RenováveisPorto-Foresti, Fábio [UNESP]Hashimoto, Diogo Teruo [UNESP]Alves, Anderson LuisAlmeida, Rodrigo Braz Castilho [UNESP]Senhorini, José AugustoBortolozzi, Jehud [UNESP]Foresti, Fausto [UNESP]2014-05-20T13:25:45Z2014-05-20T13:25:45Z2008-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article195-202application/pdfhttp://dx.doi.org/10.1590/S1415-47572008000200005Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 31, n. 1, p. 195-202, 2008.1415-4757http://hdl.handle.net/11449/818710.1590/S1415-47572008000200005S1415-47572008000200005WOS:0002572385000052-s2.0-46649084417S1415-47572008000200005.pdf3815338174165832SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Biology1.4930,638info:eu-repo/semantics/openAccess2024-04-23T15:24:02Zoai:repositorio.unesp.br:11449/8187Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:22:09.262008Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cytogenetic markers as diagnoses in the identification of the hybrid between Piauçu (Leporinus macrocephalus) and Piapara (Leporinus elongatus) |
title |
Cytogenetic markers as diagnoses in the identification of the hybrid between Piauçu (Leporinus macrocephalus) and Piapara (Leporinus elongatus) |
spellingShingle |
Cytogenetic markers as diagnoses in the identification of the hybrid between Piauçu (Leporinus macrocephalus) and Piapara (Leporinus elongatus) Porto-Foresti, Fábio [UNESP] interspecific hybrid fish culture fish cytogenetics |
title_short |
Cytogenetic markers as diagnoses in the identification of the hybrid between Piauçu (Leporinus macrocephalus) and Piapara (Leporinus elongatus) |
title_full |
Cytogenetic markers as diagnoses in the identification of the hybrid between Piauçu (Leporinus macrocephalus) and Piapara (Leporinus elongatus) |
title_fullStr |
Cytogenetic markers as diagnoses in the identification of the hybrid between Piauçu (Leporinus macrocephalus) and Piapara (Leporinus elongatus) |
title_full_unstemmed |
Cytogenetic markers as diagnoses in the identification of the hybrid between Piauçu (Leporinus macrocephalus) and Piapara (Leporinus elongatus) |
title_sort |
Cytogenetic markers as diagnoses in the identification of the hybrid between Piauçu (Leporinus macrocephalus) and Piapara (Leporinus elongatus) |
author |
Porto-Foresti, Fábio [UNESP] |
author_facet |
Porto-Foresti, Fábio [UNESP] Hashimoto, Diogo Teruo [UNESP] Alves, Anderson Luis Almeida, Rodrigo Braz Castilho [UNESP] Senhorini, José Augusto Bortolozzi, Jehud [UNESP] Foresti, Fausto [UNESP] |
author_role |
author |
author2 |
Hashimoto, Diogo Teruo [UNESP] Alves, Anderson Luis Almeida, Rodrigo Braz Castilho [UNESP] Senhorini, José Augusto Bortolozzi, Jehud [UNESP] Foresti, Fausto [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Estadual de Campinas (UNICAMP) Instituto Brasileiro do Meio Ambiente e dos Recursos Naturais Renováveis |
dc.contributor.author.fl_str_mv |
Porto-Foresti, Fábio [UNESP] Hashimoto, Diogo Teruo [UNESP] Alves, Anderson Luis Almeida, Rodrigo Braz Castilho [UNESP] Senhorini, José Augusto Bortolozzi, Jehud [UNESP] Foresti, Fausto [UNESP] |
dc.subject.por.fl_str_mv |
interspecific hybrid fish culture fish cytogenetics |
topic |
interspecific hybrid fish culture fish cytogenetics |
description |
The genetic monitoring of interspecific hybrids involves the application of methodologies able to provide an easy and indubitable genetic characterization of both parental and hybrid individuals. In the present work, cytogenetic techniques were used to identify a hybrid lineage of Piaupara in order to caracterize them in relation to the parental species, Leporinus macrocephalus (piauçu) and L. elongatus (piapara). The cytogenetic analysis revealed that L. macrocephalus presented 2n = 54 chromosomes and a nucleolar organizer regions (NOR) at the telomere of the long arm of the submetacentric chromosome pair 2. Analysis of constitutive heterochromatin (C-banding) revealed a conspicuous block at the pericentromeric region on the long arm of a submetacentric chromosome pair. L. elongatus presented the same diploid number, 2n = 54, and a karyotypic formula similar to that of L. macrocephalus. The NORs were also at the telomere of the long arm of the submetacentric pair 2, which was morphologically different from that of L. macrocephalus. Heterochromatic blocks were observed at both telomeres of a submetacentric chromosome pair. The hybrid Piaupara presented the same diploid number (2n = 54) and karyotypic formula as the parental species and there were no visible differences between parental and hybrid individuals. Differently from the Giemsa staining, NOR- and C-banding analysis showed marked differences which allowed the identification of the hybrids by the different morphology and/or size of the chromosomes carrying the NORs and patterns of heterochromatin distribution in their chromosomes. Such genetic studies are important for fish culture since they can provide tools for monitoring natural and artificial hybridization. They are also useful in biological conservation programmes and in the proper management of natural and reared fish stocks. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-01-01 2014-05-20T13:25:45Z 2014-05-20T13:25:45Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1415-47572008000200005 Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 31, n. 1, p. 195-202, 2008. 1415-4757 http://hdl.handle.net/11449/8187 10.1590/S1415-47572008000200005 S1415-47572008000200005 WOS:000257238500005 2-s2.0-46649084417 S1415-47572008000200005.pdf 3815338174165832 |
url |
http://dx.doi.org/10.1590/S1415-47572008000200005 http://hdl.handle.net/11449/8187 |
identifier_str_mv |
Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 31, n. 1, p. 195-202, 2008. 1415-4757 10.1590/S1415-47572008000200005 S1415-47572008000200005 WOS:000257238500005 2-s2.0-46649084417 S1415-47572008000200005.pdf 3815338174165832 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genetics and Molecular Biology 1.493 0,638 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
195-202 application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128239885877248 |