Use of autogenous bone and beta-tricalcium phosphate in maxillary sinus lifting: histomorphometric study and immunohistochemical assessment of RUNX2 and VEGF
Autor(a) principal: | |
---|---|
Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.ijom.2017.01.002 http://hdl.handle.net/11449/169425 |
Resumo: | The aim of this study was to compare the use of beta-tricalcium phosphate (β-TCP) (chronOS) with autogenous bone grafts alone in maxillary sinus elevation surgery. The test samples were β-TCP alone, β-TCP mixed with autogenous bone grafts (1:1), and autogenous bone grafts alone. Twelve maxillary sinuses were grafted with β-TCP (group 1), nine with β-TCP + autogenous bone graft (group 2), and 12 with autogenous bone graft (group 3). After 6 months, biopsies were obtained concurrent to the placement of dental implants; these were subjected to histomorphometric analysis and immunohistochemical analysis for runt-related transcription factor 2 (RUNX2) and vascular endothelial growth factor (VEGF). The average bone formation in group 1 was 46.3 ± 11.6% in the pristine bone region, 47.6 ± 9.9% in the intermediate region, and 44.8 ± 22.1% in the apical region; in group 2, values were 35.0 ± 15.8%, 32.5 ± 13.7%, and 32.8 ± 16.0%, respectively; in group 3, values were 43.1 ± 16.0%, 31.0 ± 13.0%, and 46.1 ± 16.3%, respectively. Immunostaining of samples in group 2 showed high cellular activity and immature bone; this differed from groups 1 and 3, in which mature bone was demonstrated. Thus, this study showed that β-TCP presents the same behaviour as autogenous bone graft, which makes it a good bone substitute. |
id |
UNSP_68cc1ef29191775d4808da3f0d34789c |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/169425 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Use of autogenous bone and beta-tricalcium phosphate in maxillary sinus lifting: histomorphometric study and immunohistochemical assessment of RUNX2 and VEGFbone graftimmunohistochemistrymaxillary sinusThe aim of this study was to compare the use of beta-tricalcium phosphate (β-TCP) (chronOS) with autogenous bone grafts alone in maxillary sinus elevation surgery. The test samples were β-TCP alone, β-TCP mixed with autogenous bone grafts (1:1), and autogenous bone grafts alone. Twelve maxillary sinuses were grafted with β-TCP (group 1), nine with β-TCP + autogenous bone graft (group 2), and 12 with autogenous bone graft (group 3). After 6 months, biopsies were obtained concurrent to the placement of dental implants; these were subjected to histomorphometric analysis and immunohistochemical analysis for runt-related transcription factor 2 (RUNX2) and vascular endothelial growth factor (VEGF). The average bone formation in group 1 was 46.3 ± 11.6% in the pristine bone region, 47.6 ± 9.9% in the intermediate region, and 44.8 ± 22.1% in the apical region; in group 2, values were 35.0 ± 15.8%, 32.5 ± 13.7%, and 32.8 ± 16.0%, respectively; in group 3, values were 43.1 ± 16.0%, 31.0 ± 13.0%, and 46.1 ± 16.3%, respectively. Immunostaining of samples in group 2 showed high cellular activity and immature bone; this differed from groups 1 and 3, in which mature bone was demonstrated. Thus, this study showed that β-TCP presents the same behaviour as autogenous bone graft, which makes it a good bone substitute.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Araçatuba Dental School Universidade Estadual Paulista – UNESPAraraquara Dental School Universidade Estadual Paulista – UNESPAraçatuba Dental School Universidade Estadual Paulista – UNESPAraraquara Dental School Universidade Estadual Paulista – UNESPFAPESP: 2012/08455-4FAPESP: 2012/10028-7Universidade Estadual Paulista (Unesp)Pereira, R. S. [UNESP]Gorla, L. F. [UNESP]Boos, F. B.J.D. [UNESP]Okamoto, R. [UNESP]Garcia Júnior, I. R. [UNESP]Hochuli-Vieira, E. [UNESP]2018-12-11T16:45:50Z2018-12-11T16:45:50Z2017-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article503-510application/pdfhttp://dx.doi.org/10.1016/j.ijom.2017.01.002International Journal of Oral and Maxillofacial Surgery, v. 46, n. 4, p. 503-510, 2017.1399-00200901-5027http://hdl.handle.net/11449/16942510.1016/j.ijom.2017.01.0022-s2.0-850115864472-s2.0-85011586447.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal of Oral and Maxillofacial Surgery1,137info:eu-repo/semantics/openAccess2023-10-27T06:11:00Zoai:repositorio.unesp.br:11449/169425Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-27T06:11Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Use of autogenous bone and beta-tricalcium phosphate in maxillary sinus lifting: histomorphometric study and immunohistochemical assessment of RUNX2 and VEGF |
title |
Use of autogenous bone and beta-tricalcium phosphate in maxillary sinus lifting: histomorphometric study and immunohistochemical assessment of RUNX2 and VEGF |
spellingShingle |
Use of autogenous bone and beta-tricalcium phosphate in maxillary sinus lifting: histomorphometric study and immunohistochemical assessment of RUNX2 and VEGF Pereira, R. S. [UNESP] bone graft immunohistochemistry maxillary sinus |
title_short |
Use of autogenous bone and beta-tricalcium phosphate in maxillary sinus lifting: histomorphometric study and immunohistochemical assessment of RUNX2 and VEGF |
title_full |
Use of autogenous bone and beta-tricalcium phosphate in maxillary sinus lifting: histomorphometric study and immunohistochemical assessment of RUNX2 and VEGF |
title_fullStr |
Use of autogenous bone and beta-tricalcium phosphate in maxillary sinus lifting: histomorphometric study and immunohistochemical assessment of RUNX2 and VEGF |
title_full_unstemmed |
Use of autogenous bone and beta-tricalcium phosphate in maxillary sinus lifting: histomorphometric study and immunohistochemical assessment of RUNX2 and VEGF |
title_sort |
Use of autogenous bone and beta-tricalcium phosphate in maxillary sinus lifting: histomorphometric study and immunohistochemical assessment of RUNX2 and VEGF |
author |
Pereira, R. S. [UNESP] |
author_facet |
Pereira, R. S. [UNESP] Gorla, L. F. [UNESP] Boos, F. B.J.D. [UNESP] Okamoto, R. [UNESP] Garcia Júnior, I. R. [UNESP] Hochuli-Vieira, E. [UNESP] |
author_role |
author |
author2 |
Gorla, L. F. [UNESP] Boos, F. B.J.D. [UNESP] Okamoto, R. [UNESP] Garcia Júnior, I. R. [UNESP] Hochuli-Vieira, E. [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Pereira, R. S. [UNESP] Gorla, L. F. [UNESP] Boos, F. B.J.D. [UNESP] Okamoto, R. [UNESP] Garcia Júnior, I. R. [UNESP] Hochuli-Vieira, E. [UNESP] |
dc.subject.por.fl_str_mv |
bone graft immunohistochemistry maxillary sinus |
topic |
bone graft immunohistochemistry maxillary sinus |
description |
The aim of this study was to compare the use of beta-tricalcium phosphate (β-TCP) (chronOS) with autogenous bone grafts alone in maxillary sinus elevation surgery. The test samples were β-TCP alone, β-TCP mixed with autogenous bone grafts (1:1), and autogenous bone grafts alone. Twelve maxillary sinuses were grafted with β-TCP (group 1), nine with β-TCP + autogenous bone graft (group 2), and 12 with autogenous bone graft (group 3). After 6 months, biopsies were obtained concurrent to the placement of dental implants; these were subjected to histomorphometric analysis and immunohistochemical analysis for runt-related transcription factor 2 (RUNX2) and vascular endothelial growth factor (VEGF). The average bone formation in group 1 was 46.3 ± 11.6% in the pristine bone region, 47.6 ± 9.9% in the intermediate region, and 44.8 ± 22.1% in the apical region; in group 2, values were 35.0 ± 15.8%, 32.5 ± 13.7%, and 32.8 ± 16.0%, respectively; in group 3, values were 43.1 ± 16.0%, 31.0 ± 13.0%, and 46.1 ± 16.3%, respectively. Immunostaining of samples in group 2 showed high cellular activity and immature bone; this differed from groups 1 and 3, in which mature bone was demonstrated. Thus, this study showed that β-TCP presents the same behaviour as autogenous bone graft, which makes it a good bone substitute. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-04-01 2018-12-11T16:45:50Z 2018-12-11T16:45:50Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.ijom.2017.01.002 International Journal of Oral and Maxillofacial Surgery, v. 46, n. 4, p. 503-510, 2017. 1399-0020 0901-5027 http://hdl.handle.net/11449/169425 10.1016/j.ijom.2017.01.002 2-s2.0-85011586447 2-s2.0-85011586447.pdf |
url |
http://dx.doi.org/10.1016/j.ijom.2017.01.002 http://hdl.handle.net/11449/169425 |
identifier_str_mv |
International Journal of Oral and Maxillofacial Surgery, v. 46, n. 4, p. 503-510, 2017. 1399-0020 0901-5027 10.1016/j.ijom.2017.01.002 2-s2.0-85011586447 2-s2.0-85011586447.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal of Oral and Maxillofacial Surgery 1,137 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
503-510 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797789530337574912 |