Effect of Aloe vera extract on the improvement of the respiratory activity of leukocytes of matrinxã during the transport stress
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1516-35982012001000023 http://hdl.handle.net/11449/623 |
Resumo: | This study evaluated the effect of extract of Aloe vera in the transport water of matrinxã (Brycon amazonicus) fish on stress response and leukocyte respiratory activity. Fish was transported for 4 h in water containing Aloe at levels 0; 0.02; 0.2 and 2 mg/L, and sampled before transport 2, 4, 24 and 96 h after for determination of plasma glucose and respiratory activity of leukocytes. An additional in vitro assay was conducted with another fish species, pacu (Piaractus mesopotamicus), to test the respiratory burst of leukocytes exposed to Aloe extract (0.0, phosphate-buffered saline (PBS) only) at 0.1, 0.2, 0.5 and 1 mg/L). Plasma glucose increased after 2 and 4 h of transport and returned to control levels within 24 h, but the addition of Aloe in the transport water did not affect the level of blood glucose. However, at 2 h of transport, Aloe enhanced the respiratory activity of leukocytes in a dose-dependent way. The highest value of respiratory burst activity of leukocytes was observed in the fish transported in water containing Aloe at 2 mg/L. The enhancing effect of the plant extract on the production of oxygen radicals was confirmed in vitro in leukocytes of pacu incubated in Aloe at concentrations 0.1 and 0.2 mg/L. The results suggest that Aloe vera is a modulator of the immune system in fish improving the innate immune response tested. |
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Repositório Institucional da UNESP |
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Effect of Aloe vera extract on the improvement of the respiratory activity of leukocytes of matrinxã during the transport stressBrycon amazonicusinnate immune systemoxidative burstwhite blood cellsThis study evaluated the effect of extract of Aloe vera in the transport water of matrinxã (Brycon amazonicus) fish on stress response and leukocyte respiratory activity. Fish was transported for 4 h in water containing Aloe at levels 0; 0.02; 0.2 and 2 mg/L, and sampled before transport 2, 4, 24 and 96 h after for determination of plasma glucose and respiratory activity of leukocytes. An additional in vitro assay was conducted with another fish species, pacu (Piaractus mesopotamicus), to test the respiratory burst of leukocytes exposed to Aloe extract (0.0, phosphate-buffered saline (PBS) only) at 0.1, 0.2, 0.5 and 1 mg/L). Plasma glucose increased after 2 and 4 h of transport and returned to control levels within 24 h, but the addition of Aloe in the transport water did not affect the level of blood glucose. However, at 2 h of transport, Aloe enhanced the respiratory activity of leukocytes in a dose-dependent way. The highest value of respiratory burst activity of leukocytes was observed in the fish transported in water containing Aloe at 2 mg/L. The enhancing effect of the plant extract on the production of oxygen radicals was confirmed in vitro in leukocytes of pacu incubated in Aloe at concentrations 0.1 and 0.2 mg/L. The results suggest that Aloe vera is a modulator of the immune system in fish improving the innate immune response tested.UNESP Faculdade de Ciências Agrárias e Veterinárias Centro de AquiculturaUNESP Faculdade de Ciências Agrárias e Veterinárias Centro de AquiculturaSociedade Brasileira de ZootecniaUniversidade Estadual Paulista (Unesp)Zanuzzo, Fábio Sabbadin [UNESP]Biller-Takahashi, Jaqueline Dalbello [UNESP]Urbinati, Elisabeth Criscuolo [UNESP]2014-05-20T13:12:43Z2014-05-20T13:12:43Z2012-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2299-2302application/pdfhttp://dx.doi.org/10.1590/S1516-35982012001000023Revista Brasileira de Zootecnia. Sociedade Brasileira de Zootecnia, v. 41, n. 10, p. 2299-2302, 2012.1516-3598http://hdl.handle.net/11449/62310.1590/S1516-35982012001000023S1516-35982012001000023WOS:000311563100023S1516-35982012001000023.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira de Zootecnia0,337info:eu-repo/semantics/openAccess2024-04-09T15:10:25Zoai:repositorio.unesp.br:11449/623Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T13:41:11.791187Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effect of Aloe vera extract on the improvement of the respiratory activity of leukocytes of matrinxã during the transport stress |
title |
Effect of Aloe vera extract on the improvement of the respiratory activity of leukocytes of matrinxã during the transport stress |
spellingShingle |
Effect of Aloe vera extract on the improvement of the respiratory activity of leukocytes of matrinxã during the transport stress Zanuzzo, Fábio Sabbadin [UNESP] Brycon amazonicus innate immune system oxidative burst white blood cells |
title_short |
Effect of Aloe vera extract on the improvement of the respiratory activity of leukocytes of matrinxã during the transport stress |
title_full |
Effect of Aloe vera extract on the improvement of the respiratory activity of leukocytes of matrinxã during the transport stress |
title_fullStr |
Effect of Aloe vera extract on the improvement of the respiratory activity of leukocytes of matrinxã during the transport stress |
title_full_unstemmed |
Effect of Aloe vera extract on the improvement of the respiratory activity of leukocytes of matrinxã during the transport stress |
title_sort |
Effect of Aloe vera extract on the improvement of the respiratory activity of leukocytes of matrinxã during the transport stress |
author |
Zanuzzo, Fábio Sabbadin [UNESP] |
author_facet |
Zanuzzo, Fábio Sabbadin [UNESP] Biller-Takahashi, Jaqueline Dalbello [UNESP] Urbinati, Elisabeth Criscuolo [UNESP] |
author_role |
author |
author2 |
Biller-Takahashi, Jaqueline Dalbello [UNESP] Urbinati, Elisabeth Criscuolo [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Zanuzzo, Fábio Sabbadin [UNESP] Biller-Takahashi, Jaqueline Dalbello [UNESP] Urbinati, Elisabeth Criscuolo [UNESP] |
dc.subject.por.fl_str_mv |
Brycon amazonicus innate immune system oxidative burst white blood cells |
topic |
Brycon amazonicus innate immune system oxidative burst white blood cells |
description |
This study evaluated the effect of extract of Aloe vera in the transport water of matrinxã (Brycon amazonicus) fish on stress response and leukocyte respiratory activity. Fish was transported for 4 h in water containing Aloe at levels 0; 0.02; 0.2 and 2 mg/L, and sampled before transport 2, 4, 24 and 96 h after for determination of plasma glucose and respiratory activity of leukocytes. An additional in vitro assay was conducted with another fish species, pacu (Piaractus mesopotamicus), to test the respiratory burst of leukocytes exposed to Aloe extract (0.0, phosphate-buffered saline (PBS) only) at 0.1, 0.2, 0.5 and 1 mg/L). Plasma glucose increased after 2 and 4 h of transport and returned to control levels within 24 h, but the addition of Aloe in the transport water did not affect the level of blood glucose. However, at 2 h of transport, Aloe enhanced the respiratory activity of leukocytes in a dose-dependent way. The highest value of respiratory burst activity of leukocytes was observed in the fish transported in water containing Aloe at 2 mg/L. The enhancing effect of the plant extract on the production of oxygen radicals was confirmed in vitro in leukocytes of pacu incubated in Aloe at concentrations 0.1 and 0.2 mg/L. The results suggest that Aloe vera is a modulator of the immune system in fish improving the innate immune response tested. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-10-01 2014-05-20T13:12:43Z 2014-05-20T13:12:43Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1516-35982012001000023 Revista Brasileira de Zootecnia. Sociedade Brasileira de Zootecnia, v. 41, n. 10, p. 2299-2302, 2012. 1516-3598 http://hdl.handle.net/11449/623 10.1590/S1516-35982012001000023 S1516-35982012001000023 WOS:000311563100023 S1516-35982012001000023.pdf |
url |
http://dx.doi.org/10.1590/S1516-35982012001000023 http://hdl.handle.net/11449/623 |
identifier_str_mv |
Revista Brasileira de Zootecnia. Sociedade Brasileira de Zootecnia, v. 41, n. 10, p. 2299-2302, 2012. 1516-3598 10.1590/S1516-35982012001000023 S1516-35982012001000023 WOS:000311563100023 S1516-35982012001000023.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista Brasileira de Zootecnia 0,337 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
2299-2302 application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Zootecnia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Zootecnia |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128264032485376 |