Avaliação de diferentes métodos no diagnóstico laboratorial de Giardia lamblia

• Autor(a) principal: Garcia, J. G D [UNESP]
• Data de Publicação: 2006
• Outros Autores: Simões, Maria Jacira Silva [UNESP], Alvarenga, Vera Lucy de Santi [UNESP]
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/389; http://hdl.handle.net/11449/69344
• topic: Coprotest®; Diagnostic methods; Direct modified method; Faust; Giardia lamblia; Iron hematoxylin; hematoxylin; iron; Brazil; correlation analysis; diagnostic test; feces analysis; giardiasis; groups by age; human; intestine infection; major clinical study; parasite; sex ratio

The protozoan parasite Giardia lamblia is responsible for a common intestinal infection in all regions of the world. In this study, four laboratory tests were evaluated for diagnostic reproducibility in the detection of this infection: Coprotest®, Direct modified method, Faust method and iron-hematoxylin staining. Positive diagnoses were tested for association with factors such as the age group and gender of the subject and the month when the sample was taken. Feces of 200 children in the Araraquara region (SP, Brazil) were examined by all four methods and the results compared. G. lamblia was found to be the most frequent parasite and 8.0% of the children showed giardiasis. There was no apparent correlation with gender, but most of the parasites were found in three- to five-year-olds. The highest frequency of infection occurred in January. The most reliable diagnostic results for G. lamblia were achieved by combining at least two methods of good reproducibility, i.e. Coprotest-Faust, Direct-Faust or Coprotest-Direct (A > 0.81).