Mechanical Stability and Proteolytic Activity of Resin-dentin Bonds Using the Cross-linked Dry Bonding Technique

Detalhes bibliográficos
Autor(a) principal: Citta, M. [UNESP]
Data de Publicação: 2021
Outros Autores: Anovazzi, G. [UNESP], Basso, F. G. [UNESP], Scheffel, D. L. S., Zhou, J., Pashley, D. H., Souza Costa, C. A. [UNESP], Hebling, J. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.2341/20-016-L
http://hdl.handle.net/11449/218426
Resumo: Objective: To evaluate the mechanical stability and the proteolytic activity of bonds created by a two-step, etch-and-rinse adhesive applied to cross-linked and air-dried etched dentin. Methods: Flat dentin surfaces were produced in 64 extracted sound human molars. The dentin was etched with 35% phosphoric acid for 15 seconds, and then the teeth were divided into groups according to the cross-linking solution applied on the etched dentin. Group 1: 5% grape seed extract (GSE), Group 2: 5% glutaraldehyde, Group 3: Gluma Desensitizer, or Group 4: deionized water (control). Solutions were applied for 60 seconds, followed by rinse and blot drying. Then, the teeth were separated into two subgroups where the etched dentin was kept moist or air-dried. The adhesive was applied followed by a composite resin buildup. After 24 hours, the teeth were cut into beams (0.81 mm(2)) that were tested for microtensile strength immediately or after 12 months of aging in a 37 degrees C saliva-like buffer. Additional teeth ( n=32) were bonded as described and cut into 0.5-mm-thick slabs. The slabs were prepared for nanoleakage (scanning electron microscopy) and in situ zymography (EnzChek Protease Assay Kit). Bond strength data were submitted to ANOVA and Tukey tests (alpha=0.05). Results: Significant reduction in immediate bond strength (Ca 65%) and increase in proteolytic activity was seen when the etched dentin was air dried without previous cross-linking biomodification. Conversely, bond strengths did not differ from those produced on wet dentin when collagen was cross-linked before air drying, irrespective of the solution applied. For both moist and air-dried etched dentin, collagen cross-linking resulted in mechanically stable bonds and reduced proteolytic activity after 12 months of storage. Conclusion: Bonds produced by the application of a two-step, etch-and-rinse adhesive to cross-linked, air-dried, etched dentin were mechanically stable and revealed reduced proteolytic activity after 1 year of aging.
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spelling Mechanical Stability and Proteolytic Activity of Resin-dentin Bonds Using the Cross-linked Dry Bonding TechniqueObjective: To evaluate the mechanical stability and the proteolytic activity of bonds created by a two-step, etch-and-rinse adhesive applied to cross-linked and air-dried etched dentin. Methods: Flat dentin surfaces were produced in 64 extracted sound human molars. The dentin was etched with 35% phosphoric acid for 15 seconds, and then the teeth were divided into groups according to the cross-linking solution applied on the etched dentin. Group 1: 5% grape seed extract (GSE), Group 2: 5% glutaraldehyde, Group 3: Gluma Desensitizer, or Group 4: deionized water (control). Solutions were applied for 60 seconds, followed by rinse and blot drying. Then, the teeth were separated into two subgroups where the etched dentin was kept moist or air-dried. The adhesive was applied followed by a composite resin buildup. After 24 hours, the teeth were cut into beams (0.81 mm(2)) that were tested for microtensile strength immediately or after 12 months of aging in a 37 degrees C saliva-like buffer. Additional teeth ( n=32) were bonded as described and cut into 0.5-mm-thick slabs. The slabs were prepared for nanoleakage (scanning electron microscopy) and in situ zymography (EnzChek Protease Assay Kit). Bond strength data were submitted to ANOVA and Tukey tests (alpha=0.05). Results: Significant reduction in immediate bond strength (Ca 65%) and increase in proteolytic activity was seen when the etched dentin was air dried without previous cross-linking biomodification. Conversely, bond strengths did not differ from those produced on wet dentin when collagen was cross-linked before air drying, irrespective of the solution applied. For both moist and air-dried etched dentin, collagen cross-linking resulted in mechanically stable bonds and reduced proteolytic activity after 12 months of storage. Conclusion: Bonds produced by the application of a two-step, etch-and-rinse adhesive to cross-linked, air-dried, etched dentin were mechanically stable and revealed reduced proteolytic activity after 1 year of aging.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Sao Paulo State Univ UNESP, Sch Dent, Dept Oral Rehabil, Sao Paulo, SP, BrazilSao Paulo State Univ UNESP, Sch Dent, Dept Orthodont & Pediat Dent, Sao Paulo, SP, BrazilUniv Estadual Maringa, Sch Dent, Dept Dent, Maringa, Parana, BrazilPeking Univ, Dept Prosthodont, Sch & Hosp Stomatol, Beijing, Peoples R ChinaAugusta Univ, Dent Coll Georgia, Dept Oral Biol, Augusta, GA USASao Paulo State Univ UNESP, Sch Dent, Dept Physiol & Pathol, Sao Paulo, SP, BrazilSao Paulo State Univ UNESP, Sch Dent, Dept Oral Rehabil, Sao Paulo, SP, BrazilSao Paulo State Univ UNESP, Sch Dent, Dept Orthodont & Pediat Dent, Sao Paulo, SP, BrazilSao Paulo State Univ UNESP, Sch Dent, Dept Physiol & Pathol, Sao Paulo, SP, BrazilFAPESP: 2017/03927-9CNPq: 427256/20160CAPES: 001Operative Dentistry IncUniversidade Estadual Paulista (UNESP)Universidade Estadual de Maringá (UEM)Peking UnivAugusta UnivCitta, M. [UNESP]Anovazzi, G. [UNESP]Basso, F. G. [UNESP]Scheffel, D. L. S.Zhou, J.Pashley, D. H.Souza Costa, C. A. [UNESP]Hebling, J. [UNESP]2022-04-28T17:20:57Z2022-04-28T17:20:57Z2021-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article13http://dx.doi.org/10.2341/20-016-LOperative Dentistry. Indianapolis: Operative Dentistry Inc, v. 46, n. 6, 13 p., 2021.0361-7734http://hdl.handle.net/11449/21842610.2341/20-016-LWOS:000731546500005Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengOperative Dentistryinfo:eu-repo/semantics/openAccess2022-04-28T17:20:57Zoai:repositorio.unesp.br:11449/218426Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:52:16.852107Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Mechanical Stability and Proteolytic Activity of Resin-dentin Bonds Using the Cross-linked Dry Bonding Technique
title Mechanical Stability and Proteolytic Activity of Resin-dentin Bonds Using the Cross-linked Dry Bonding Technique
spellingShingle Mechanical Stability and Proteolytic Activity of Resin-dentin Bonds Using the Cross-linked Dry Bonding Technique
Citta, M. [UNESP]
title_short Mechanical Stability and Proteolytic Activity of Resin-dentin Bonds Using the Cross-linked Dry Bonding Technique
title_full Mechanical Stability and Proteolytic Activity of Resin-dentin Bonds Using the Cross-linked Dry Bonding Technique
title_fullStr Mechanical Stability and Proteolytic Activity of Resin-dentin Bonds Using the Cross-linked Dry Bonding Technique
title_full_unstemmed Mechanical Stability and Proteolytic Activity of Resin-dentin Bonds Using the Cross-linked Dry Bonding Technique
title_sort Mechanical Stability and Proteolytic Activity of Resin-dentin Bonds Using the Cross-linked Dry Bonding Technique
author Citta, M. [UNESP]
author_facet Citta, M. [UNESP]
Anovazzi, G. [UNESP]
Basso, F. G. [UNESP]
Scheffel, D. L. S.
Zhou, J.
Pashley, D. H.
Souza Costa, C. A. [UNESP]
Hebling, J. [UNESP]
author_role author
author2 Anovazzi, G. [UNESP]
Basso, F. G. [UNESP]
Scheffel, D. L. S.
Zhou, J.
Pashley, D. H.
Souza Costa, C. A. [UNESP]
Hebling, J. [UNESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Universidade Estadual de Maringá (UEM)
Peking Univ
Augusta Univ
dc.contributor.author.fl_str_mv Citta, M. [UNESP]
Anovazzi, G. [UNESP]
Basso, F. G. [UNESP]
Scheffel, D. L. S.
Zhou, J.
Pashley, D. H.
Souza Costa, C. A. [UNESP]
Hebling, J. [UNESP]
description Objective: To evaluate the mechanical stability and the proteolytic activity of bonds created by a two-step, etch-and-rinse adhesive applied to cross-linked and air-dried etched dentin. Methods: Flat dentin surfaces were produced in 64 extracted sound human molars. The dentin was etched with 35% phosphoric acid for 15 seconds, and then the teeth were divided into groups according to the cross-linking solution applied on the etched dentin. Group 1: 5% grape seed extract (GSE), Group 2: 5% glutaraldehyde, Group 3: Gluma Desensitizer, or Group 4: deionized water (control). Solutions were applied for 60 seconds, followed by rinse and blot drying. Then, the teeth were separated into two subgroups where the etched dentin was kept moist or air-dried. The adhesive was applied followed by a composite resin buildup. After 24 hours, the teeth were cut into beams (0.81 mm(2)) that were tested for microtensile strength immediately or after 12 months of aging in a 37 degrees C saliva-like buffer. Additional teeth ( n=32) were bonded as described and cut into 0.5-mm-thick slabs. The slabs were prepared for nanoleakage (scanning electron microscopy) and in situ zymography (EnzChek Protease Assay Kit). Bond strength data were submitted to ANOVA and Tukey tests (alpha=0.05). Results: Significant reduction in immediate bond strength (Ca 65%) and increase in proteolytic activity was seen when the etched dentin was air dried without previous cross-linking biomodification. Conversely, bond strengths did not differ from those produced on wet dentin when collagen was cross-linked before air drying, irrespective of the solution applied. For both moist and air-dried etched dentin, collagen cross-linking resulted in mechanically stable bonds and reduced proteolytic activity after 12 months of storage. Conclusion: Bonds produced by the application of a two-step, etch-and-rinse adhesive to cross-linked, air-dried, etched dentin were mechanically stable and revealed reduced proteolytic activity after 1 year of aging.
publishDate 2021
dc.date.none.fl_str_mv 2021-11-01
2022-04-28T17:20:57Z
2022-04-28T17:20:57Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.2341/20-016-L
Operative Dentistry. Indianapolis: Operative Dentistry Inc, v. 46, n. 6, 13 p., 2021.
0361-7734
http://hdl.handle.net/11449/218426
10.2341/20-016-L
WOS:000731546500005
url http://dx.doi.org/10.2341/20-016-L
http://hdl.handle.net/11449/218426
identifier_str_mv Operative Dentistry. Indianapolis: Operative Dentistry Inc, v. 46, n. 6, 13 p., 2021.
0361-7734
10.2341/20-016-L
WOS:000731546500005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Operative Dentistry
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv 13
dc.publisher.none.fl_str_mv Operative Dentistry Inc
publisher.none.fl_str_mv Operative Dentistry Inc
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
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repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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