Mechanical Stability and Proteolytic Activity of Resin-dentin Bonds Using the Cross-linked Dry Bonding Technique
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.2341/20-016-L http://hdl.handle.net/11449/218426 |
Resumo: | Objective: To evaluate the mechanical stability and the proteolytic activity of bonds created by a two-step, etch-and-rinse adhesive applied to cross-linked and air-dried etched dentin. Methods: Flat dentin surfaces were produced in 64 extracted sound human molars. The dentin was etched with 35% phosphoric acid for 15 seconds, and then the teeth were divided into groups according to the cross-linking solution applied on the etched dentin. Group 1: 5% grape seed extract (GSE), Group 2: 5% glutaraldehyde, Group 3: Gluma Desensitizer, or Group 4: deionized water (control). Solutions were applied for 60 seconds, followed by rinse and blot drying. Then, the teeth were separated into two subgroups where the etched dentin was kept moist or air-dried. The adhesive was applied followed by a composite resin buildup. After 24 hours, the teeth were cut into beams (0.81 mm(2)) that were tested for microtensile strength immediately or after 12 months of aging in a 37 degrees C saliva-like buffer. Additional teeth ( n=32) were bonded as described and cut into 0.5-mm-thick slabs. The slabs were prepared for nanoleakage (scanning electron microscopy) and in situ zymography (EnzChek Protease Assay Kit). Bond strength data were submitted to ANOVA and Tukey tests (alpha=0.05). Results: Significant reduction in immediate bond strength (Ca 65%) and increase in proteolytic activity was seen when the etched dentin was air dried without previous cross-linking biomodification. Conversely, bond strengths did not differ from those produced on wet dentin when collagen was cross-linked before air drying, irrespective of the solution applied. For both moist and air-dried etched dentin, collagen cross-linking resulted in mechanically stable bonds and reduced proteolytic activity after 12 months of storage. Conclusion: Bonds produced by the application of a two-step, etch-and-rinse adhesive to cross-linked, air-dried, etched dentin were mechanically stable and revealed reduced proteolytic activity after 1 year of aging. |
id |
UNSP_68fe50ed4da953a12eb4b60efd660c2e |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/218426 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Mechanical Stability and Proteolytic Activity of Resin-dentin Bonds Using the Cross-linked Dry Bonding TechniqueObjective: To evaluate the mechanical stability and the proteolytic activity of bonds created by a two-step, etch-and-rinse adhesive applied to cross-linked and air-dried etched dentin. Methods: Flat dentin surfaces were produced in 64 extracted sound human molars. The dentin was etched with 35% phosphoric acid for 15 seconds, and then the teeth were divided into groups according to the cross-linking solution applied on the etched dentin. Group 1: 5% grape seed extract (GSE), Group 2: 5% glutaraldehyde, Group 3: Gluma Desensitizer, or Group 4: deionized water (control). Solutions were applied for 60 seconds, followed by rinse and blot drying. Then, the teeth were separated into two subgroups where the etched dentin was kept moist or air-dried. The adhesive was applied followed by a composite resin buildup. After 24 hours, the teeth were cut into beams (0.81 mm(2)) that were tested for microtensile strength immediately or after 12 months of aging in a 37 degrees C saliva-like buffer. Additional teeth ( n=32) were bonded as described and cut into 0.5-mm-thick slabs. The slabs were prepared for nanoleakage (scanning electron microscopy) and in situ zymography (EnzChek Protease Assay Kit). Bond strength data were submitted to ANOVA and Tukey tests (alpha=0.05). Results: Significant reduction in immediate bond strength (Ca 65%) and increase in proteolytic activity was seen when the etched dentin was air dried without previous cross-linking biomodification. Conversely, bond strengths did not differ from those produced on wet dentin when collagen was cross-linked before air drying, irrespective of the solution applied. For both moist and air-dried etched dentin, collagen cross-linking resulted in mechanically stable bonds and reduced proteolytic activity after 12 months of storage. Conclusion: Bonds produced by the application of a two-step, etch-and-rinse adhesive to cross-linked, air-dried, etched dentin were mechanically stable and revealed reduced proteolytic activity after 1 year of aging.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Sao Paulo State Univ UNESP, Sch Dent, Dept Oral Rehabil, Sao Paulo, SP, BrazilSao Paulo State Univ UNESP, Sch Dent, Dept Orthodont & Pediat Dent, Sao Paulo, SP, BrazilUniv Estadual Maringa, Sch Dent, Dept Dent, Maringa, Parana, BrazilPeking Univ, Dept Prosthodont, Sch & Hosp Stomatol, Beijing, Peoples R ChinaAugusta Univ, Dent Coll Georgia, Dept Oral Biol, Augusta, GA USASao Paulo State Univ UNESP, Sch Dent, Dept Physiol & Pathol, Sao Paulo, SP, BrazilSao Paulo State Univ UNESP, Sch Dent, Dept Oral Rehabil, Sao Paulo, SP, BrazilSao Paulo State Univ UNESP, Sch Dent, Dept Orthodont & Pediat Dent, Sao Paulo, SP, BrazilSao Paulo State Univ UNESP, Sch Dent, Dept Physiol & Pathol, Sao Paulo, SP, BrazilFAPESP: 2017/03927-9CNPq: 427256/20160CAPES: 001Operative Dentistry IncUniversidade Estadual Paulista (UNESP)Universidade Estadual de Maringá (UEM)Peking UnivAugusta UnivCitta, M. [UNESP]Anovazzi, G. [UNESP]Basso, F. G. [UNESP]Scheffel, D. L. S.Zhou, J.Pashley, D. H.Souza Costa, C. A. [UNESP]Hebling, J. [UNESP]2022-04-28T17:20:57Z2022-04-28T17:20:57Z2021-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article13http://dx.doi.org/10.2341/20-016-LOperative Dentistry. Indianapolis: Operative Dentistry Inc, v. 46, n. 6, 13 p., 2021.0361-7734http://hdl.handle.net/11449/21842610.2341/20-016-LWOS:000731546500005Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengOperative Dentistryinfo:eu-repo/semantics/openAccess2022-04-28T17:20:57Zoai:repositorio.unesp.br:11449/218426Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:52:16.852107Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Mechanical Stability and Proteolytic Activity of Resin-dentin Bonds Using the Cross-linked Dry Bonding Technique |
title |
Mechanical Stability and Proteolytic Activity of Resin-dentin Bonds Using the Cross-linked Dry Bonding Technique |
spellingShingle |
Mechanical Stability and Proteolytic Activity of Resin-dentin Bonds Using the Cross-linked Dry Bonding Technique Citta, M. [UNESP] |
title_short |
Mechanical Stability and Proteolytic Activity of Resin-dentin Bonds Using the Cross-linked Dry Bonding Technique |
title_full |
Mechanical Stability and Proteolytic Activity of Resin-dentin Bonds Using the Cross-linked Dry Bonding Technique |
title_fullStr |
Mechanical Stability and Proteolytic Activity of Resin-dentin Bonds Using the Cross-linked Dry Bonding Technique |
title_full_unstemmed |
Mechanical Stability and Proteolytic Activity of Resin-dentin Bonds Using the Cross-linked Dry Bonding Technique |
title_sort |
Mechanical Stability and Proteolytic Activity of Resin-dentin Bonds Using the Cross-linked Dry Bonding Technique |
author |
Citta, M. [UNESP] |
author_facet |
Citta, M. [UNESP] Anovazzi, G. [UNESP] Basso, F. G. [UNESP] Scheffel, D. L. S. Zhou, J. Pashley, D. H. Souza Costa, C. A. [UNESP] Hebling, J. [UNESP] |
author_role |
author |
author2 |
Anovazzi, G. [UNESP] Basso, F. G. [UNESP] Scheffel, D. L. S. Zhou, J. Pashley, D. H. Souza Costa, C. A. [UNESP] Hebling, J. [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade Estadual de Maringá (UEM) Peking Univ Augusta Univ |
dc.contributor.author.fl_str_mv |
Citta, M. [UNESP] Anovazzi, G. [UNESP] Basso, F. G. [UNESP] Scheffel, D. L. S. Zhou, J. Pashley, D. H. Souza Costa, C. A. [UNESP] Hebling, J. [UNESP] |
description |
Objective: To evaluate the mechanical stability and the proteolytic activity of bonds created by a two-step, etch-and-rinse adhesive applied to cross-linked and air-dried etched dentin. Methods: Flat dentin surfaces were produced in 64 extracted sound human molars. The dentin was etched with 35% phosphoric acid for 15 seconds, and then the teeth were divided into groups according to the cross-linking solution applied on the etched dentin. Group 1: 5% grape seed extract (GSE), Group 2: 5% glutaraldehyde, Group 3: Gluma Desensitizer, or Group 4: deionized water (control). Solutions were applied for 60 seconds, followed by rinse and blot drying. Then, the teeth were separated into two subgroups where the etched dentin was kept moist or air-dried. The adhesive was applied followed by a composite resin buildup. After 24 hours, the teeth were cut into beams (0.81 mm(2)) that were tested for microtensile strength immediately or after 12 months of aging in a 37 degrees C saliva-like buffer. Additional teeth ( n=32) were bonded as described and cut into 0.5-mm-thick slabs. The slabs were prepared for nanoleakage (scanning electron microscopy) and in situ zymography (EnzChek Protease Assay Kit). Bond strength data were submitted to ANOVA and Tukey tests (alpha=0.05). Results: Significant reduction in immediate bond strength (Ca 65%) and increase in proteolytic activity was seen when the etched dentin was air dried without previous cross-linking biomodification. Conversely, bond strengths did not differ from those produced on wet dentin when collagen was cross-linked before air drying, irrespective of the solution applied. For both moist and air-dried etched dentin, collagen cross-linking resulted in mechanically stable bonds and reduced proteolytic activity after 12 months of storage. Conclusion: Bonds produced by the application of a two-step, etch-and-rinse adhesive to cross-linked, air-dried, etched dentin were mechanically stable and revealed reduced proteolytic activity after 1 year of aging. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-11-01 2022-04-28T17:20:57Z 2022-04-28T17:20:57Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.2341/20-016-L Operative Dentistry. Indianapolis: Operative Dentistry Inc, v. 46, n. 6, 13 p., 2021. 0361-7734 http://hdl.handle.net/11449/218426 10.2341/20-016-L WOS:000731546500005 |
url |
http://dx.doi.org/10.2341/20-016-L http://hdl.handle.net/11449/218426 |
identifier_str_mv |
Operative Dentistry. Indianapolis: Operative Dentistry Inc, v. 46, n. 6, 13 p., 2021. 0361-7734 10.2341/20-016-L WOS:000731546500005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Operative Dentistry |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
13 |
dc.publisher.none.fl_str_mv |
Operative Dentistry Inc |
publisher.none.fl_str_mv |
Operative Dentistry Inc |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129260073779200 |