Salivary proteomic profile of young adults before and after the practice of interval exercise: preliminary results
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s11332-021-00883-z http://hdl.handle.net/11449/231594 |
Resumo: | The practice of exercise can change biological markers. Thus, the use of saliva to define possible biomarkers has increased with the advances in “omics” techniques. Therefore, this study analyzed the salivary proteomic profile in young adults before and after interval exercise. Five adults, without comorbidities and with normal salivary flow, aged between 20 and 35 years (3 men and 2 women) were selected. The collection of stimulated (SS) and non-stimulated (NSS) saliva was done before (BE) and after (AE) exercise, which consisted of interval exercise with 5 min walking on a treadmill and 5 min at rest, during a total of 40 min. The proteins were extracted individually and processed by label-free proteomics. There was no significant difference between BE and AE salivary flows. For SS, 69 and 60 proteins were found BE (12 exclusives) and AE (3 exclusives), respectively. Ten subunits of immunoglobulins (8 more than twofold), as well as Lysozyme C and Cystatin-S, were increased AE compared to BE. Six hemoglobin’s isoforms were decreased AE compared to BE. For NSS, 76 and 65 proteins were found BE (18 exclusives) and AE (7 exclusives), respectively. Albumin was increased AE, while Basic salivary proline-rich protein 1 and 2 decreased. Apolipoprotein A-I was identified exclusively AE compared to BE. The salivary proteome after interval exercise suggests increase of immunity. |
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Salivary proteomic profile of young adults before and after the practice of interval exercise: preliminary resultsBiomarkersInterval exerciseProteinsProteomicsSalivaSalivary flowsThe practice of exercise can change biological markers. Thus, the use of saliva to define possible biomarkers has increased with the advances in “omics” techniques. Therefore, this study analyzed the salivary proteomic profile in young adults before and after interval exercise. Five adults, without comorbidities and with normal salivary flow, aged between 20 and 35 years (3 men and 2 women) were selected. The collection of stimulated (SS) and non-stimulated (NSS) saliva was done before (BE) and after (AE) exercise, which consisted of interval exercise with 5 min walking on a treadmill and 5 min at rest, during a total of 40 min. The proteins were extracted individually and processed by label-free proteomics. There was no significant difference between BE and AE salivary flows. For SS, 69 and 60 proteins were found BE (12 exclusives) and AE (3 exclusives), respectively. Ten subunits of immunoglobulins (8 more than twofold), as well as Lysozyme C and Cystatin-S, were increased AE compared to BE. Six hemoglobin’s isoforms were decreased AE compared to BE. For NSS, 76 and 65 proteins were found BE (18 exclusives) and AE (7 exclusives), respectively. Albumin was increased AE, while Basic salivary proline-rich protein 1 and 2 decreased. Apolipoprotein A-I was identified exclusively AE compared to BE. The salivary proteome after interval exercise suggests increase of immunity.Department of Biological Sciences Bauru School of Dentistry University of São Paulo, Al. Octávio Pinheiro Brisolla, 9-75, SPGraduate Program in Movement Sciences Department of Physical Education Human Movement Research Laboratory (MOVI-LAB) School of Sciences São Paulo State University (UNESP), SPGraduate Program in Movement Sciences Department of Physical Education Human Movement Research Laboratory (MOVI-LAB) School of Sciences São Paulo State University (UNESP), SPUniversidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)Ventura, Talita Mendes OliveiraSantos, Karina OliveiraBraga, Aline SilvaThomassian, Larissa Tercilia GrizzoBuzalaf, Marília Afonso RabeloBarbieri, Fábio Augusto [UNESP]Kalva-Filho, Carlos Augusto [UNESP]Faria, Murilo Henrique [UNESP]Magalhães, Ana Carolina2022-04-29T08:46:19Z2022-04-29T08:46:19Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s11332-021-00883-zSport Sciences for Health.1825-12341824-7490http://hdl.handle.net/11449/23159410.1007/s11332-021-00883-z2-s2.0-85122892018Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengSport Sciences for Healthinfo:eu-repo/semantics/openAccess2024-04-23T15:24:02Zoai:repositorio.unesp.br:11449/231594Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:01:43.938514Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Salivary proteomic profile of young adults before and after the practice of interval exercise: preliminary results |
title |
Salivary proteomic profile of young adults before and after the practice of interval exercise: preliminary results |
spellingShingle |
Salivary proteomic profile of young adults before and after the practice of interval exercise: preliminary results Ventura, Talita Mendes Oliveira Biomarkers Interval exercise Proteins Proteomics Saliva Salivary flows |
title_short |
Salivary proteomic profile of young adults before and after the practice of interval exercise: preliminary results |
title_full |
Salivary proteomic profile of young adults before and after the practice of interval exercise: preliminary results |
title_fullStr |
Salivary proteomic profile of young adults before and after the practice of interval exercise: preliminary results |
title_full_unstemmed |
Salivary proteomic profile of young adults before and after the practice of interval exercise: preliminary results |
title_sort |
Salivary proteomic profile of young adults before and after the practice of interval exercise: preliminary results |
author |
Ventura, Talita Mendes Oliveira |
author_facet |
Ventura, Talita Mendes Oliveira Santos, Karina Oliveira Braga, Aline Silva Thomassian, Larissa Tercilia Grizzo Buzalaf, Marília Afonso Rabelo Barbieri, Fábio Augusto [UNESP] Kalva-Filho, Carlos Augusto [UNESP] Faria, Murilo Henrique [UNESP] Magalhães, Ana Carolina |
author_role |
author |
author2 |
Santos, Karina Oliveira Braga, Aline Silva Thomassian, Larissa Tercilia Grizzo Buzalaf, Marília Afonso Rabelo Barbieri, Fábio Augusto [UNESP] Kalva-Filho, Carlos Augusto [UNESP] Faria, Murilo Henrique [UNESP] Magalhães, Ana Carolina |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Ventura, Talita Mendes Oliveira Santos, Karina Oliveira Braga, Aline Silva Thomassian, Larissa Tercilia Grizzo Buzalaf, Marília Afonso Rabelo Barbieri, Fábio Augusto [UNESP] Kalva-Filho, Carlos Augusto [UNESP] Faria, Murilo Henrique [UNESP] Magalhães, Ana Carolina |
dc.subject.por.fl_str_mv |
Biomarkers Interval exercise Proteins Proteomics Saliva Salivary flows |
topic |
Biomarkers Interval exercise Proteins Proteomics Saliva Salivary flows |
description |
The practice of exercise can change biological markers. Thus, the use of saliva to define possible biomarkers has increased with the advances in “omics” techniques. Therefore, this study analyzed the salivary proteomic profile in young adults before and after interval exercise. Five adults, without comorbidities and with normal salivary flow, aged between 20 and 35 years (3 men and 2 women) were selected. The collection of stimulated (SS) and non-stimulated (NSS) saliva was done before (BE) and after (AE) exercise, which consisted of interval exercise with 5 min walking on a treadmill and 5 min at rest, during a total of 40 min. The proteins were extracted individually and processed by label-free proteomics. There was no significant difference between BE and AE salivary flows. For SS, 69 and 60 proteins were found BE (12 exclusives) and AE (3 exclusives), respectively. Ten subunits of immunoglobulins (8 more than twofold), as well as Lysozyme C and Cystatin-S, were increased AE compared to BE. Six hemoglobin’s isoforms were decreased AE compared to BE. For NSS, 76 and 65 proteins were found BE (18 exclusives) and AE (7 exclusives), respectively. Albumin was increased AE, while Basic salivary proline-rich protein 1 and 2 decreased. Apolipoprotein A-I was identified exclusively AE compared to BE. The salivary proteome after interval exercise suggests increase of immunity. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-29T08:46:19Z 2022-04-29T08:46:19Z 2022-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s11332-021-00883-z Sport Sciences for Health. 1825-1234 1824-7490 http://hdl.handle.net/11449/231594 10.1007/s11332-021-00883-z 2-s2.0-85122892018 |
url |
http://dx.doi.org/10.1007/s11332-021-00883-z http://hdl.handle.net/11449/231594 |
identifier_str_mv |
Sport Sciences for Health. 1825-1234 1824-7490 10.1007/s11332-021-00883-z 2-s2.0-85122892018 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Sport Sciences for Health |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129574135922688 |