Produção de anticorpos IGY anti-Photobacterium damselae subsp. piscicida uso terapêutico e profilático em Rachycentron canadum

• Main Author: Eto, Silas Fernandes [UNESP]
• Publication Date: 2015
• Format: Doctoral thesis
• Language: por
• Source: Repositório Institucional da UNESP
• Download full: http://hdl.handle.net/11449/143026; http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/14-07-2016/000864610.pdf
• topic: Peixe; Imunoterapia; Imunoglobulinas; Septicemia; Vacinas; Bactérias; Immunotherapy

The Photobacterium damselae ssp. piscicida is an important causative agent of septicemia and pseudotuberculosis in the acute and chronic phases in marine fish, respectively. The method for immunization and treatment of bacterial infections in fish is limited to the active immunization, which demands cellular bioenergy to generate humoral immune response. The use of antibiotics in the water as a form of therapy results in residues in the meat that will be consumed by humans, favoring the development of microbial resistance. Thus, the aim of the study was to produce and purify polyclonal IgY, specific for membrane proteins of P. damselae ssp. piscicida (PMBphdp), extracted from the yolk of laying hens, and to observe its effects on the control of acute sepsis in experimentally infected Rachycentron canadus. The results showed reduction of cell accumulation in acute inflammation induced by inoculation of the homologous bacteria in the coelomic cavity and decrease in the respiratory activity of blood leukocytes in groups receiving IgY antibodies at 48 hours and 15 days before the challenge. It was also observed the reduction of bacterial dissemination to the liver, kidney and heart which was correlated with the decrease in liver and kidney damage, which was demonstrated by the biochemical and histopathological profile. The results are promising regarding the use of immunoglobulin Y as prophylactic method and for immunotherapy of microbial infections in aquatic organisms