Differential expression on mitochondrial tryparedoxin peroxidase (mTcTXNPx) in Trypanosoma cruzi after ferrocenyl diamine hydrochlorides treatments
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.bjid.2016.10.010 http://hdl.handle.net/11449/162639 |
Resumo: | Resistance to benznidazole in certain strains of Trypanosoma cruzi may be caused by the increased production of enzymes that act on the oxidative metabolism, such as mitochondrial tryparedoxin peroxidase which catalyses the reduction of peroxides. This work presents cytotoxicity assays performed with ferrocenyl diamine hydrochlorides in six different strains of T. cruzi epimastigote forms (Y, Bolivia, SI1, SI8, QMII, and SIGR3). The last four strains have been recently isolated from triatominae and mammalian host (domestic cat). The expression of mitochondrial tryparedoxin peroxidase was analyzed by the Western blotting technique using polyclonal antibody anti mitochondrial tryparedoxin peroxidase obtained from a rabbit immunized with the mitochondrial tryparedoxin peroxidase recombinant protein. All the tested ferrocenyl diamine hydrochlorides were more cytotoxic than benznidazole. The expression of the 25.5 kDa polypeptide of mitochondrial tryparedoxin peroxidase did not increase in strains that were more resistant to the ferrocenyl compounds (SI8 and SIGR3). In addition, a 58 kDa polypeptide was also recognized in all strains. Ferrocenyl diamine hydrochlorides showed trypanocidal activity and the expression of 25.5 kDa mitochondrial tryparedoxin peroxidase is not necessarily increased in some T. cruzi strains. Most likely, other mechanisms, in addition to the over expression of this antioxidative enzyme, should be involved in the escape of parasites from cytotoxic oxidant agents. (C) 2016 Sociedade Brasileira de Infectologia. Published by Elsevier Editora Ltda. This is an open access article under the CC BY-NC-ND license. |
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Differential expression on mitochondrial tryparedoxin peroxidase (mTcTXNPx) in Trypanosoma cruzi after ferrocenyl diamine hydrochlorides treatmentsTrypanosoma cruziFerrocenyl diamine hydrochloridesMitochondrial tryparedoxinperoxidase (mTcTXNPx)Oxidative stress mechanismsResistance to benznidazole in certain strains of Trypanosoma cruzi may be caused by the increased production of enzymes that act on the oxidative metabolism, such as mitochondrial tryparedoxin peroxidase which catalyses the reduction of peroxides. This work presents cytotoxicity assays performed with ferrocenyl diamine hydrochlorides in six different strains of T. cruzi epimastigote forms (Y, Bolivia, SI1, SI8, QMII, and SIGR3). The last four strains have been recently isolated from triatominae and mammalian host (domestic cat). The expression of mitochondrial tryparedoxin peroxidase was analyzed by the Western blotting technique using polyclonal antibody anti mitochondrial tryparedoxin peroxidase obtained from a rabbit immunized with the mitochondrial tryparedoxin peroxidase recombinant protein. All the tested ferrocenyl diamine hydrochlorides were more cytotoxic than benznidazole. The expression of the 25.5 kDa polypeptide of mitochondrial tryparedoxin peroxidase did not increase in strains that were more resistant to the ferrocenyl compounds (SI8 and SIGR3). In addition, a 58 kDa polypeptide was also recognized in all strains. Ferrocenyl diamine hydrochlorides showed trypanocidal activity and the expression of 25.5 kDa mitochondrial tryparedoxin peroxidase is not necessarily increased in some T. cruzi strains. Most likely, other mechanisms, in addition to the over expression of this antioxidative enzyme, should be involved in the escape of parasites from cytotoxic oxidant agents. (C) 2016 Sociedade Brasileira de Infectologia. Published by Elsevier Editora Ltda. This is an open access article under the CC BY-NC-ND license.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Fundação para o Desenvolvimento da UNESP (FUNDUNESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)PADC/FCFArUniv Estadual Paulista, Lab Imunol & Biol Mol, Dept Ciencias Biol, Araraquara, SP, BrazilUniv Fed Fluminense, Inst Quim, Niteroi, RJ, BrazilUniv Estadual Paulista, Lab Parasitol, Dept Ciencias Biol, Araraquara, SP, BrazilUniv Sao Paulo, Inst Fis, Sao Carlos, SP, BrazilUniv Estadual Paulista, Lab Imunol & Biol Mol, Dept Ciencias Biol, Araraquara, SP, BrazilUniv Estadual Paulista, Lab Parasitol, Dept Ciencias Biol, Araraquara, SP, BrazilFAPESP: 2011/06525-2Elsevier B.V.Universidade Estadual Paulista (Unesp)Universidade Federal Fluminense (UFF)Universidade de São Paulo (USP)Kohatsu, Andrea A. N. [UNESP]Silva, Flavia A. J. [UNESP]Francisco, Acacio I.Rimoldi, Aline [UNESP]Silva, Marco T. A.Vargas, Maria D.Rosa, Joao A. da [UNESP]Cicarelli, Regina M. B. [UNESP]2018-11-26T17:24:15Z2018-11-26T17:24:15Z2017-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article125-132application/pdfhttp://dx.doi.org/10.1016/j.bjid.2016.10.010Brazilian Journal Of Infectious Diseases. Rio De Janeiro: Elsevier Brazil, v. 21, n. 2, p. 125-132, 2017.1413-8670http://hdl.handle.net/11449/16263910.1016/j.bjid.2016.10.010WOS:000397963900002WOS000397963900002.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal Of Infectious Diseases0,817info:eu-repo/semantics/openAccess2023-10-01T06:00:57Zoai:repositorio.unesp.br:11449/162639Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-01T06:00:57Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Differential expression on mitochondrial tryparedoxin peroxidase (mTcTXNPx) in Trypanosoma cruzi after ferrocenyl diamine hydrochlorides treatments |
title |
Differential expression on mitochondrial tryparedoxin peroxidase (mTcTXNPx) in Trypanosoma cruzi after ferrocenyl diamine hydrochlorides treatments |
spellingShingle |
Differential expression on mitochondrial tryparedoxin peroxidase (mTcTXNPx) in Trypanosoma cruzi after ferrocenyl diamine hydrochlorides treatments Kohatsu, Andrea A. N. [UNESP] Trypanosoma cruzi Ferrocenyl diamine hydrochlorides Mitochondrial tryparedoxin peroxidase (mTcTXNPx) Oxidative stress mechanisms |
title_short |
Differential expression on mitochondrial tryparedoxin peroxidase (mTcTXNPx) in Trypanosoma cruzi after ferrocenyl diamine hydrochlorides treatments |
title_full |
Differential expression on mitochondrial tryparedoxin peroxidase (mTcTXNPx) in Trypanosoma cruzi after ferrocenyl diamine hydrochlorides treatments |
title_fullStr |
Differential expression on mitochondrial tryparedoxin peroxidase (mTcTXNPx) in Trypanosoma cruzi after ferrocenyl diamine hydrochlorides treatments |
title_full_unstemmed |
Differential expression on mitochondrial tryparedoxin peroxidase (mTcTXNPx) in Trypanosoma cruzi after ferrocenyl diamine hydrochlorides treatments |
title_sort |
Differential expression on mitochondrial tryparedoxin peroxidase (mTcTXNPx) in Trypanosoma cruzi after ferrocenyl diamine hydrochlorides treatments |
author |
Kohatsu, Andrea A. N. [UNESP] |
author_facet |
Kohatsu, Andrea A. N. [UNESP] Silva, Flavia A. J. [UNESP] Francisco, Acacio I. Rimoldi, Aline [UNESP] Silva, Marco T. A. Vargas, Maria D. Rosa, Joao A. da [UNESP] Cicarelli, Regina M. B. [UNESP] |
author_role |
author |
author2 |
Silva, Flavia A. J. [UNESP] Francisco, Acacio I. Rimoldi, Aline [UNESP] Silva, Marco T. A. Vargas, Maria D. Rosa, Joao A. da [UNESP] Cicarelli, Regina M. B. [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal Fluminense (UFF) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Kohatsu, Andrea A. N. [UNESP] Silva, Flavia A. J. [UNESP] Francisco, Acacio I. Rimoldi, Aline [UNESP] Silva, Marco T. A. Vargas, Maria D. Rosa, Joao A. da [UNESP] Cicarelli, Regina M. B. [UNESP] |
dc.subject.por.fl_str_mv |
Trypanosoma cruzi Ferrocenyl diamine hydrochlorides Mitochondrial tryparedoxin peroxidase (mTcTXNPx) Oxidative stress mechanisms |
topic |
Trypanosoma cruzi Ferrocenyl diamine hydrochlorides Mitochondrial tryparedoxin peroxidase (mTcTXNPx) Oxidative stress mechanisms |
description |
Resistance to benznidazole in certain strains of Trypanosoma cruzi may be caused by the increased production of enzymes that act on the oxidative metabolism, such as mitochondrial tryparedoxin peroxidase which catalyses the reduction of peroxides. This work presents cytotoxicity assays performed with ferrocenyl diamine hydrochlorides in six different strains of T. cruzi epimastigote forms (Y, Bolivia, SI1, SI8, QMII, and SIGR3). The last four strains have been recently isolated from triatominae and mammalian host (domestic cat). The expression of mitochondrial tryparedoxin peroxidase was analyzed by the Western blotting technique using polyclonal antibody anti mitochondrial tryparedoxin peroxidase obtained from a rabbit immunized with the mitochondrial tryparedoxin peroxidase recombinant protein. All the tested ferrocenyl diamine hydrochlorides were more cytotoxic than benznidazole. The expression of the 25.5 kDa polypeptide of mitochondrial tryparedoxin peroxidase did not increase in strains that were more resistant to the ferrocenyl compounds (SI8 and SIGR3). In addition, a 58 kDa polypeptide was also recognized in all strains. Ferrocenyl diamine hydrochlorides showed trypanocidal activity and the expression of 25.5 kDa mitochondrial tryparedoxin peroxidase is not necessarily increased in some T. cruzi strains. Most likely, other mechanisms, in addition to the over expression of this antioxidative enzyme, should be involved in the escape of parasites from cytotoxic oxidant agents. (C) 2016 Sociedade Brasileira de Infectologia. Published by Elsevier Editora Ltda. This is an open access article under the CC BY-NC-ND license. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-03-01 2018-11-26T17:24:15Z 2018-11-26T17:24:15Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.bjid.2016.10.010 Brazilian Journal Of Infectious Diseases. Rio De Janeiro: Elsevier Brazil, v. 21, n. 2, p. 125-132, 2017. 1413-8670 http://hdl.handle.net/11449/162639 10.1016/j.bjid.2016.10.010 WOS:000397963900002 WOS000397963900002.pdf |
url |
http://dx.doi.org/10.1016/j.bjid.2016.10.010 http://hdl.handle.net/11449/162639 |
identifier_str_mv |
Brazilian Journal Of Infectious Diseases. Rio De Janeiro: Elsevier Brazil, v. 21, n. 2, p. 125-132, 2017. 1413-8670 10.1016/j.bjid.2016.10.010 WOS:000397963900002 WOS000397963900002.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal Of Infectious Diseases 0,817 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
125-132 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964384315834368 |