Citotoxicity of Fipronil on Hepatocytes Isolated from Rat and Effects of Its Biotransformation
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1516-89132015060298 http://hdl.handle.net/11449/161033 |
Resumo: | The aim of this study was to characterize the mechanism of toxicity of fipronil on hepatocytes isolated from the rat and the effect of its biotransformation on the toxicological potential. The toxicity of fipronil was assessed by monitoring the oxygen consumption and mitochondrial membrane potential, intracellular ATP concentration, Ca2+ homeostasis and cell viability. The cell viability was evaluated by trypan blue exclusion in hepatocytes that were isolated from the normal rats and by the release of the enzymes alanine transaminase and aspartate transaminase in hepatocytes that were isolated from the normal rats or proadifen-pretreated rats. Fipronil reduced mitochondrial respiration in the cells that were energized with glutamate plus malate in a dose-dependent manner and dissipated the mitochondrial membrane potential that was accompanied by a reduction in ATP concentration and a disruption of intracellular Ca2+ homeostasis. The cell viability was affected by fipronil with higher potency in hepatocytes that were isolated from the normal rats, which indicated that the metabolism of this insecticide increased its toxicological potential. The results of this study indicated that the toxicity of fipronil to the hepatocytes was related to the inhibition of mitochondrial activity, which led to decreased ATP synthesis and a consequent alteration in intracellular Ca2+ homeostasis and ultimately resulted in cell death. |
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Citotoxicity of Fipronil on Hepatocytes Isolated from Rat and Effects of Its Biotransformationfipronilhepatotoxicitybioenergeticsbiotransformationcalcium homeostasisThe aim of this study was to characterize the mechanism of toxicity of fipronil on hepatocytes isolated from the rat and the effect of its biotransformation on the toxicological potential. The toxicity of fipronil was assessed by monitoring the oxygen consumption and mitochondrial membrane potential, intracellular ATP concentration, Ca2+ homeostasis and cell viability. The cell viability was evaluated by trypan blue exclusion in hepatocytes that were isolated from the normal rats and by the release of the enzymes alanine transaminase and aspartate transaminase in hepatocytes that were isolated from the normal rats or proadifen-pretreated rats. Fipronil reduced mitochondrial respiration in the cells that were energized with glutamate plus malate in a dose-dependent manner and dissipated the mitochondrial membrane potential that was accompanied by a reduction in ATP concentration and a disruption of intracellular Ca2+ homeostasis. The cell viability was affected by fipronil with higher potency in hepatocytes that were isolated from the normal rats, which indicated that the metabolism of this insecticide increased its toxicological potential. The results of this study indicated that the toxicity of fipronil to the hepatocytes was related to the inhibition of mitochondrial activity, which led to decreased ATP synthesis and a consequent alteration in intracellular Ca2+ homeostasis and ultimately resulted in cell death.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista, Dept Zootecnia, Dracena, SP, BrazilUniv Estadual Paulista, Prod & Saude Anim, Aracatuba, SP, BrazilUniv Estadual Paulista, Dept Zootecnia, Dracena, SP, BrazilUniv Estadual Paulista, Prod & Saude Anim, Aracatuba, SP, BrazilFAPESP: 2012/15135-6FAPESP: 2012/24872-4Inst Tecnologia ParanaUniversidade Estadual Paulista (Unesp)Guelfi, Marieli [UNESP]Maioli, Marcos Antonio [UNESP]Tavares, Marco Aurelio [UNESP]Mingatto, Fabio Erminio [UNESP]2018-11-26T16:18:53Z2018-11-26T16:18:53Z2015-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article843-853application/pdfhttp://dx.doi.org/10.1590/S1516-89132015060298Brazilian Archives Of Biology And Technology. Curitiba-parana: Inst Tecnologia Parana, v. 58, n. 6, p. 843-853, 2015.1516-8913http://hdl.handle.net/11449/16103310.1590/S1516-89132015060298S1516-89132015000600843WOS:000366277400004S1516-89132015000600843.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Archives Of Biology And Technology0,281info:eu-repo/semantics/openAccess2024-09-04T19:16:19Zoai:repositorio.unesp.br:11449/161033Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T19:16:19Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Citotoxicity of Fipronil on Hepatocytes Isolated from Rat and Effects of Its Biotransformation |
title |
Citotoxicity of Fipronil on Hepatocytes Isolated from Rat and Effects of Its Biotransformation |
spellingShingle |
Citotoxicity of Fipronil on Hepatocytes Isolated from Rat and Effects of Its Biotransformation Guelfi, Marieli [UNESP] fipronil hepatotoxicity bioenergetics biotransformation calcium homeostasis |
title_short |
Citotoxicity of Fipronil on Hepatocytes Isolated from Rat and Effects of Its Biotransformation |
title_full |
Citotoxicity of Fipronil on Hepatocytes Isolated from Rat and Effects of Its Biotransformation |
title_fullStr |
Citotoxicity of Fipronil on Hepatocytes Isolated from Rat and Effects of Its Biotransformation |
title_full_unstemmed |
Citotoxicity of Fipronil on Hepatocytes Isolated from Rat and Effects of Its Biotransformation |
title_sort |
Citotoxicity of Fipronil on Hepatocytes Isolated from Rat and Effects of Its Biotransformation |
author |
Guelfi, Marieli [UNESP] |
author_facet |
Guelfi, Marieli [UNESP] Maioli, Marcos Antonio [UNESP] Tavares, Marco Aurelio [UNESP] Mingatto, Fabio Erminio [UNESP] |
author_role |
author |
author2 |
Maioli, Marcos Antonio [UNESP] Tavares, Marco Aurelio [UNESP] Mingatto, Fabio Erminio [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Guelfi, Marieli [UNESP] Maioli, Marcos Antonio [UNESP] Tavares, Marco Aurelio [UNESP] Mingatto, Fabio Erminio [UNESP] |
dc.subject.por.fl_str_mv |
fipronil hepatotoxicity bioenergetics biotransformation calcium homeostasis |
topic |
fipronil hepatotoxicity bioenergetics biotransformation calcium homeostasis |
description |
The aim of this study was to characterize the mechanism of toxicity of fipronil on hepatocytes isolated from the rat and the effect of its biotransformation on the toxicological potential. The toxicity of fipronil was assessed by monitoring the oxygen consumption and mitochondrial membrane potential, intracellular ATP concentration, Ca2+ homeostasis and cell viability. The cell viability was evaluated by trypan blue exclusion in hepatocytes that were isolated from the normal rats and by the release of the enzymes alanine transaminase and aspartate transaminase in hepatocytes that were isolated from the normal rats or proadifen-pretreated rats. Fipronil reduced mitochondrial respiration in the cells that were energized with glutamate plus malate in a dose-dependent manner and dissipated the mitochondrial membrane potential that was accompanied by a reduction in ATP concentration and a disruption of intracellular Ca2+ homeostasis. The cell viability was affected by fipronil with higher potency in hepatocytes that were isolated from the normal rats, which indicated that the metabolism of this insecticide increased its toxicological potential. The results of this study indicated that the toxicity of fipronil to the hepatocytes was related to the inhibition of mitochondrial activity, which led to decreased ATP synthesis and a consequent alteration in intracellular Ca2+ homeostasis and ultimately resulted in cell death. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-11-01 2018-11-26T16:18:53Z 2018-11-26T16:18:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1516-89132015060298 Brazilian Archives Of Biology And Technology. Curitiba-parana: Inst Tecnologia Parana, v. 58, n. 6, p. 843-853, 2015. 1516-8913 http://hdl.handle.net/11449/161033 10.1590/S1516-89132015060298 S1516-89132015000600843 WOS:000366277400004 S1516-89132015000600843.pdf |
url |
http://dx.doi.org/10.1590/S1516-89132015060298 http://hdl.handle.net/11449/161033 |
identifier_str_mv |
Brazilian Archives Of Biology And Technology. Curitiba-parana: Inst Tecnologia Parana, v. 58, n. 6, p. 843-853, 2015. 1516-8913 10.1590/S1516-89132015060298 S1516-89132015000600843 WOS:000366277400004 S1516-89132015000600843.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Archives Of Biology And Technology 0,281 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
843-853 application/pdf |
dc.publisher.none.fl_str_mv |
Inst Tecnologia Parana |
publisher.none.fl_str_mv |
Inst Tecnologia Parana |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1810021422824685568 |