Profiling the proteomics in honeybee worker brains submitted to the proboscis extension reflex
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jprot.2016.05.029 http://hdl.handle.net/11449/173040 |
Resumo: | The proboscis extension reflex (PER) is an unconditioned stimulus (US) widely used to access the ability of honeybees to correlate it with a conditioned stimulus (CS) during learning and memory acquisition. However, little is known about the biochemical/genetic changes in worker honeybee brains induced by the PER alone. The present investigation profiled the proteomic complement associated with the PER to further the understanding of the major molecular transformations in the honeybee brain during the execution of a US. In the present study, a quantitative shotgun proteomic approach was employed to assign the proteomic complement of the honeybee brain. The results were analyzed under the view of protein networking for different processes involved in PER behavior. In the brains of PER-stimulated individuals, the metabolism of cyclic/heterocyclic/aromatic compounds was activated in parallel with the metabolism of nitrogenated compounds, followed by the up-regulation of carbohydrate metabolism, the proteins involved with the anatomic and cytoskeleton; the down-regulation of the anatomic development and cell differentiation in other neurons also occurred. Significance The assay of proboscis extension reflex is frequently used to access honeybees' ability to correlate an unconditioned stimulus with a conditioned stimulus (such as an odor) to establish learning and memory acquisition. The reflex behavior of proboscis extension was associated with various conditioned stimuli, and the biochemical/genetic evaluation of the changes occurring in honeybee brains under these conditions reflect the synergistic effects of both insect manipulations (training to answer to an unconditioned stimulus and training to respond to a conditioned stimulus). Little or no information is available regarding the biochemical changes stimulated by an unconditioned stimulus alone, such as the proboscis extension reflex. The present investigation characterizes the proteomic changes occurring in the brains of honeybee workers submitted to proboscis extension reflex. A series of metabolic and cellular processes were identified to be related to the reflex of an unconditioned stimulus. This strategy may be reproduced to further understand the processes of learning and memory acquisition in honeybees. |
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Profiling the proteomics in honeybee worker brains submitted to the proboscis extension reflexHoneybeeLabel-free quantitationMemoryNeuroproteomicsShotgunThe proboscis extension reflex (PER) is an unconditioned stimulus (US) widely used to access the ability of honeybees to correlate it with a conditioned stimulus (CS) during learning and memory acquisition. However, little is known about the biochemical/genetic changes in worker honeybee brains induced by the PER alone. The present investigation profiled the proteomic complement associated with the PER to further the understanding of the major molecular transformations in the honeybee brain during the execution of a US. In the present study, a quantitative shotgun proteomic approach was employed to assign the proteomic complement of the honeybee brain. The results were analyzed under the view of protein networking for different processes involved in PER behavior. In the brains of PER-stimulated individuals, the metabolism of cyclic/heterocyclic/aromatic compounds was activated in parallel with the metabolism of nitrogenated compounds, followed by the up-regulation of carbohydrate metabolism, the proteins involved with the anatomic and cytoskeleton; the down-regulation of the anatomic development and cell differentiation in other neurons also occurred. Significance The assay of proboscis extension reflex is frequently used to access honeybees' ability to correlate an unconditioned stimulus with a conditioned stimulus (such as an odor) to establish learning and memory acquisition. The reflex behavior of proboscis extension was associated with various conditioned stimuli, and the biochemical/genetic evaluation of the changes occurring in honeybee brains under these conditions reflect the synergistic effects of both insect manipulations (training to answer to an unconditioned stimulus and training to respond to a conditioned stimulus). Little or no information is available regarding the biochemical changes stimulated by an unconditioned stimulus alone, such as the proboscis extension reflex. The present investigation characterizes the proteomic changes occurring in the brains of honeybee workers submitted to proboscis extension reflex. A series of metabolic and cellular processes were identified to be related to the reflex of an unconditioned stimulus. This strategy may be reproduced to further understand the processes of learning and memory acquisition in honeybees.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Center of the Study of Social Insects Department of Biology Institute of Biosciences of Rio Claro São Paulo State University (UNESP)Laboratory for Proteomics and Protein Engineering Carlos Chagas InstituteCenter of the Study of Social Insects Department of Biology Institute of Biosciences of Rio Claro São Paulo State University (UNESP)Universidade Estadual Paulista (Unesp)Carlos Chagas Instituteda Silva Menegasso, Anally Ribeiro [UNESP]Pratavieira, Marcel [UNESP]de Saldanha da Gama Fischer, JulianaCarvalho, Paulo CostaRoat, Thaisa Cristina [UNESP]Malaspina, Osmar [UNESP]Palma, Mario Sergio [UNESP]2018-12-11T17:03:14Z2018-12-11T17:03:14Z2017-01-16info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article131-144application/pdfhttp://dx.doi.org/10.1016/j.jprot.2016.05.029Journal of Proteomics, v. 151, p. 131-144.1876-77371874-3919http://hdl.handle.net/11449/17304010.1016/j.jprot.2016.05.0292-s2.0-849731297582-s2.0-84973129758.pdf290188862450653575385560855058190000-0002-1650-257XScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Proteomics1,430info:eu-repo/semantics/openAccess2023-10-01T06:03:02Zoai:repositorio.unesp.br:11449/173040Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T13:39:27.092170Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Profiling the proteomics in honeybee worker brains submitted to the proboscis extension reflex |
title |
Profiling the proteomics in honeybee worker brains submitted to the proboscis extension reflex |
spellingShingle |
Profiling the proteomics in honeybee worker brains submitted to the proboscis extension reflex da Silva Menegasso, Anally Ribeiro [UNESP] Honeybee Label-free quantitation Memory Neuroproteomics Shotgun |
title_short |
Profiling the proteomics in honeybee worker brains submitted to the proboscis extension reflex |
title_full |
Profiling the proteomics in honeybee worker brains submitted to the proboscis extension reflex |
title_fullStr |
Profiling the proteomics in honeybee worker brains submitted to the proboscis extension reflex |
title_full_unstemmed |
Profiling the proteomics in honeybee worker brains submitted to the proboscis extension reflex |
title_sort |
Profiling the proteomics in honeybee worker brains submitted to the proboscis extension reflex |
author |
da Silva Menegasso, Anally Ribeiro [UNESP] |
author_facet |
da Silva Menegasso, Anally Ribeiro [UNESP] Pratavieira, Marcel [UNESP] de Saldanha da Gama Fischer, Juliana Carvalho, Paulo Costa Roat, Thaisa Cristina [UNESP] Malaspina, Osmar [UNESP] Palma, Mario Sergio [UNESP] |
author_role |
author |
author2 |
Pratavieira, Marcel [UNESP] de Saldanha da Gama Fischer, Juliana Carvalho, Paulo Costa Roat, Thaisa Cristina [UNESP] Malaspina, Osmar [UNESP] Palma, Mario Sergio [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Carlos Chagas Institute |
dc.contributor.author.fl_str_mv |
da Silva Menegasso, Anally Ribeiro [UNESP] Pratavieira, Marcel [UNESP] de Saldanha da Gama Fischer, Juliana Carvalho, Paulo Costa Roat, Thaisa Cristina [UNESP] Malaspina, Osmar [UNESP] Palma, Mario Sergio [UNESP] |
dc.subject.por.fl_str_mv |
Honeybee Label-free quantitation Memory Neuroproteomics Shotgun |
topic |
Honeybee Label-free quantitation Memory Neuroproteomics Shotgun |
description |
The proboscis extension reflex (PER) is an unconditioned stimulus (US) widely used to access the ability of honeybees to correlate it with a conditioned stimulus (CS) during learning and memory acquisition. However, little is known about the biochemical/genetic changes in worker honeybee brains induced by the PER alone. The present investigation profiled the proteomic complement associated with the PER to further the understanding of the major molecular transformations in the honeybee brain during the execution of a US. In the present study, a quantitative shotgun proteomic approach was employed to assign the proteomic complement of the honeybee brain. The results were analyzed under the view of protein networking for different processes involved in PER behavior. In the brains of PER-stimulated individuals, the metabolism of cyclic/heterocyclic/aromatic compounds was activated in parallel with the metabolism of nitrogenated compounds, followed by the up-regulation of carbohydrate metabolism, the proteins involved with the anatomic and cytoskeleton; the down-regulation of the anatomic development and cell differentiation in other neurons also occurred. Significance The assay of proboscis extension reflex is frequently used to access honeybees' ability to correlate an unconditioned stimulus with a conditioned stimulus (such as an odor) to establish learning and memory acquisition. The reflex behavior of proboscis extension was associated with various conditioned stimuli, and the biochemical/genetic evaluation of the changes occurring in honeybee brains under these conditions reflect the synergistic effects of both insect manipulations (training to answer to an unconditioned stimulus and training to respond to a conditioned stimulus). Little or no information is available regarding the biochemical changes stimulated by an unconditioned stimulus alone, such as the proboscis extension reflex. The present investigation characterizes the proteomic changes occurring in the brains of honeybee workers submitted to proboscis extension reflex. A series of metabolic and cellular processes were identified to be related to the reflex of an unconditioned stimulus. This strategy may be reproduced to further understand the processes of learning and memory acquisition in honeybees. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-16 2018-12-11T17:03:14Z 2018-12-11T17:03:14Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jprot.2016.05.029 Journal of Proteomics, v. 151, p. 131-144. 1876-7737 1874-3919 http://hdl.handle.net/11449/173040 10.1016/j.jprot.2016.05.029 2-s2.0-84973129758 2-s2.0-84973129758.pdf 2901888624506535 7538556085505819 0000-0002-1650-257X |
url |
http://dx.doi.org/10.1016/j.jprot.2016.05.029 http://hdl.handle.net/11449/173040 |
identifier_str_mv |
Journal of Proteomics, v. 151, p. 131-144. 1876-7737 1874-3919 10.1016/j.jprot.2016.05.029 2-s2.0-84973129758 2-s2.0-84973129758.pdf 2901888624506535 7538556085505819 0000-0002-1650-257X |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Proteomics 1,430 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
131-144 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128258955280384 |