A fingerprint of plasma proteome alteration after local tissue damage induced by Bothrops leucurus snake venom in mice
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jprot.2021.104464 http://hdl.handle.net/11449/230125 |
Resumo: | Bothrops spp. is responsible for about 70% of snakebites in Brazil, causing a diverse and complex pathophysiological condition. Bothrops leucurus is the main species of medical relevance found in the Atlantic coast in the Brazilian Northeast region. The pathophysiological effects involved B. leucurus snakebite as well as the organism's reaction in response to this envenoming, it has not been explored yet. Thus, edema was induced in mice paw using 1.2, 2.5, and 5.0 μg of B. leucurus venom, the percentage of edema was measured 30 min after injection and the blood plasma was collected and analyzed by shotgun proteomic strategy. We identified 80 common plasma proteins with differential abundance among the experimental groups and we can understand the early aspects of this snake envenomation, regardless of the suggestive severity of an ophidian accident. The results showed B. leucurus venom triggers a thromboinflammation scenario where family's proteins of the Serpins, Apolipoproteins, Complement factors and Component subunits, Cathepsins, Kinases, Oxidoreductases, Proteases inhibitors, Proteases, Collagens, Growth factors are related to inflammation, complement and coagulation systems, modulators platelets and neutrophils, lipid and retinoid metabolism, oxidative stress and tissue repair. Our findings set precedents for future studies in the area of early diagnosis and/or treatment of snakebites. Significance: The physiopathological effects that the snake venoms can cause have been investigated through classical and reductionist tools, which allowed, so far, the identification of action mechanisms of individual components associated with specific tissue damage. The currently incomplete limitations of this knowledge must be expanded through new approaches, such as proteomics, which may represent a big leap in understanding the venom-modulated pathological process. The exploration of the complete protein set that suffer modifications by the simultaneous action of multiple toxins, provides a map of the establishment of physiopathological phenotypes, which favors the identification of multiple toxin targets, that may or may not act in synergy, as well as favoring the discovery of biomarkers and therapeutic targets for manifestations that are not neutralized by the antivenom. |
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A fingerprint of plasma proteome alteration after local tissue damage induced by Bothrops leucurus snake venom in miceEdemaInflammationMass spectrometrySnake venomThromboinflammationBothrops spp. is responsible for about 70% of snakebites in Brazil, causing a diverse and complex pathophysiological condition. Bothrops leucurus is the main species of medical relevance found in the Atlantic coast in the Brazilian Northeast region. The pathophysiological effects involved B. leucurus snakebite as well as the organism's reaction in response to this envenoming, it has not been explored yet. Thus, edema was induced in mice paw using 1.2, 2.5, and 5.0 μg of B. leucurus venom, the percentage of edema was measured 30 min after injection and the blood plasma was collected and analyzed by shotgun proteomic strategy. We identified 80 common plasma proteins with differential abundance among the experimental groups and we can understand the early aspects of this snake envenomation, regardless of the suggestive severity of an ophidian accident. The results showed B. leucurus venom triggers a thromboinflammation scenario where family's proteins of the Serpins, Apolipoproteins, Complement factors and Component subunits, Cathepsins, Kinases, Oxidoreductases, Proteases inhibitors, Proteases, Collagens, Growth factors are related to inflammation, complement and coagulation systems, modulators platelets and neutrophils, lipid and retinoid metabolism, oxidative stress and tissue repair. Our findings set precedents for future studies in the area of early diagnosis and/or treatment of snakebites. Significance: The physiopathological effects that the snake venoms can cause have been investigated through classical and reductionist tools, which allowed, so far, the identification of action mechanisms of individual components associated with specific tissue damage. The currently incomplete limitations of this knowledge must be expanded through new approaches, such as proteomics, which may represent a big leap in understanding the venom-modulated pathological process. The exploration of the complete protein set that suffer modifications by the simultaneous action of multiple toxins, provides a map of the establishment of physiopathological phenotypes, which favors the identification of multiple toxin targets, that may or may not act in synergy, as well as favoring the discovery of biomarkers and therapeutic targets for manifestations that are not neutralized by the antivenom.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Graduate Program in Tropical Diseases Botucatu Medical School (FMB) São Paulo State University (UNESP)Center of Mathematics Computing Sciences and Cognition Federal University of ABCLaboratory for Structural and Computational Proteomics ICC Oswaldo Cruz Foundation (FIOCRUZ)Drug Research and Development Center Federal University of Ceará (UFC)Center for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP)Biotechnology Institute (IBTEC) São Paulo State University (UNESP)Department of Chemical and Biological Sciences São Paulo State University (UNESP)Department of Physiology and Pharmacology School of Medicine Federal University of Ceará (UFC)Graduate Program in Tropical Diseases Botucatu Medical School (FMB) São Paulo State University (UNESP)Center for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP)Biotechnology Institute (IBTEC) São Paulo State University (UNESP)Department of Chemical and Biological Sciences São Paulo State University (UNESP)CNPq: 437089/2018-5Universidade Estadual Paulista (UNESP)Federal University of ABCOswaldo Cruz Foundation (FIOCRUZ)Federal University of Ceará (UFC)Cavalcante, Joeliton dos Santos [UNESP]de Almeida, Cayo Antônio SoaresClasen, Milan Avilada Silva, Emerson Lucenade Barros, Luciana Curtolo [UNESP]Marinho, Aline DiogoRossini, Bruno Cesar [UNESP]Marino, Celso Luís [UNESP]Carvalho, Paulo CostaJorge, Roberta Jeane Bezerrados Santos, Lucilene Delazari [UNESP]2022-04-29T08:38:03Z2022-04-29T08:38:03Z2022-02-20info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.jprot.2021.104464Journal of Proteomics, v. 253.1876-77371874-3919http://hdl.handle.net/11449/23012510.1016/j.jprot.2021.1044642-s2.0-85121900605Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Proteomicsinfo:eu-repo/semantics/openAccess2024-08-15T15:22:34Zoai:repositorio.unesp.br:11449/230125Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-15T15:22:34Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
A fingerprint of plasma proteome alteration after local tissue damage induced by Bothrops leucurus snake venom in mice |
title |
A fingerprint of plasma proteome alteration after local tissue damage induced by Bothrops leucurus snake venom in mice |
spellingShingle |
A fingerprint of plasma proteome alteration after local tissue damage induced by Bothrops leucurus snake venom in mice Cavalcante, Joeliton dos Santos [UNESP] Edema Inflammation Mass spectrometry Snake venom Thromboinflammation |
title_short |
A fingerprint of plasma proteome alteration after local tissue damage induced by Bothrops leucurus snake venom in mice |
title_full |
A fingerprint of plasma proteome alteration after local tissue damage induced by Bothrops leucurus snake venom in mice |
title_fullStr |
A fingerprint of plasma proteome alteration after local tissue damage induced by Bothrops leucurus snake venom in mice |
title_full_unstemmed |
A fingerprint of plasma proteome alteration after local tissue damage induced by Bothrops leucurus snake venom in mice |
title_sort |
A fingerprint of plasma proteome alteration after local tissue damage induced by Bothrops leucurus snake venom in mice |
author |
Cavalcante, Joeliton dos Santos [UNESP] |
author_facet |
Cavalcante, Joeliton dos Santos [UNESP] de Almeida, Cayo Antônio Soares Clasen, Milan Avila da Silva, Emerson Lucena de Barros, Luciana Curtolo [UNESP] Marinho, Aline Diogo Rossini, Bruno Cesar [UNESP] Marino, Celso Luís [UNESP] Carvalho, Paulo Costa Jorge, Roberta Jeane Bezerra dos Santos, Lucilene Delazari [UNESP] |
author_role |
author |
author2 |
de Almeida, Cayo Antônio Soares Clasen, Milan Avila da Silva, Emerson Lucena de Barros, Luciana Curtolo [UNESP] Marinho, Aline Diogo Rossini, Bruno Cesar [UNESP] Marino, Celso Luís [UNESP] Carvalho, Paulo Costa Jorge, Roberta Jeane Bezerra dos Santos, Lucilene Delazari [UNESP] |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Federal University of ABC Oswaldo Cruz Foundation (FIOCRUZ) Federal University of Ceará (UFC) |
dc.contributor.author.fl_str_mv |
Cavalcante, Joeliton dos Santos [UNESP] de Almeida, Cayo Antônio Soares Clasen, Milan Avila da Silva, Emerson Lucena de Barros, Luciana Curtolo [UNESP] Marinho, Aline Diogo Rossini, Bruno Cesar [UNESP] Marino, Celso Luís [UNESP] Carvalho, Paulo Costa Jorge, Roberta Jeane Bezerra dos Santos, Lucilene Delazari [UNESP] |
dc.subject.por.fl_str_mv |
Edema Inflammation Mass spectrometry Snake venom Thromboinflammation |
topic |
Edema Inflammation Mass spectrometry Snake venom Thromboinflammation |
description |
Bothrops spp. is responsible for about 70% of snakebites in Brazil, causing a diverse and complex pathophysiological condition. Bothrops leucurus is the main species of medical relevance found in the Atlantic coast in the Brazilian Northeast region. The pathophysiological effects involved B. leucurus snakebite as well as the organism's reaction in response to this envenoming, it has not been explored yet. Thus, edema was induced in mice paw using 1.2, 2.5, and 5.0 μg of B. leucurus venom, the percentage of edema was measured 30 min after injection and the blood plasma was collected and analyzed by shotgun proteomic strategy. We identified 80 common plasma proteins with differential abundance among the experimental groups and we can understand the early aspects of this snake envenomation, regardless of the suggestive severity of an ophidian accident. The results showed B. leucurus venom triggers a thromboinflammation scenario where family's proteins of the Serpins, Apolipoproteins, Complement factors and Component subunits, Cathepsins, Kinases, Oxidoreductases, Proteases inhibitors, Proteases, Collagens, Growth factors are related to inflammation, complement and coagulation systems, modulators platelets and neutrophils, lipid and retinoid metabolism, oxidative stress and tissue repair. Our findings set precedents for future studies in the area of early diagnosis and/or treatment of snakebites. Significance: The physiopathological effects that the snake venoms can cause have been investigated through classical and reductionist tools, which allowed, so far, the identification of action mechanisms of individual components associated with specific tissue damage. The currently incomplete limitations of this knowledge must be expanded through new approaches, such as proteomics, which may represent a big leap in understanding the venom-modulated pathological process. The exploration of the complete protein set that suffer modifications by the simultaneous action of multiple toxins, provides a map of the establishment of physiopathological phenotypes, which favors the identification of multiple toxin targets, that may or may not act in synergy, as well as favoring the discovery of biomarkers and therapeutic targets for manifestations that are not neutralized by the antivenom. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-29T08:38:03Z 2022-04-29T08:38:03Z 2022-02-20 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jprot.2021.104464 Journal of Proteomics, v. 253. 1876-7737 1874-3919 http://hdl.handle.net/11449/230125 10.1016/j.jprot.2021.104464 2-s2.0-85121900605 |
url |
http://dx.doi.org/10.1016/j.jprot.2021.104464 http://hdl.handle.net/11449/230125 |
identifier_str_mv |
Journal of Proteomics, v. 253. 1876-7737 1874-3919 10.1016/j.jprot.2021.104464 2-s2.0-85121900605 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Proteomics |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128127104188416 |