A fingerprint of plasma proteome alteration after local tissue damage induced by Bothrops leucurus snake venom in mice

Detalhes bibliográficos
Autor(a) principal: Cavalcante, Joeliton dos Santos [UNESP]
Data de Publicação: 2022
Outros Autores: de Almeida, Cayo Antônio Soares, Clasen, Milan Avila, da Silva, Emerson Lucena, de Barros, Luciana Curtolo [UNESP], Marinho, Aline Diogo, Rossini, Bruno Cesar [UNESP], Marino, Celso Luís [UNESP], Carvalho, Paulo Costa, Jorge, Roberta Jeane Bezerra, dos Santos, Lucilene Delazari [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.jprot.2021.104464
http://hdl.handle.net/11449/230125
Resumo: Bothrops spp. is responsible for about 70% of snakebites in Brazil, causing a diverse and complex pathophysiological condition. Bothrops leucurus is the main species of medical relevance found in the Atlantic coast in the Brazilian Northeast region. The pathophysiological effects involved B. leucurus snakebite as well as the organism's reaction in response to this envenoming, it has not been explored yet. Thus, edema was induced in mice paw using 1.2, 2.5, and 5.0 μg of B. leucurus venom, the percentage of edema was measured 30 min after injection and the blood plasma was collected and analyzed by shotgun proteomic strategy. We identified 80 common plasma proteins with differential abundance among the experimental groups and we can understand the early aspects of this snake envenomation, regardless of the suggestive severity of an ophidian accident. The results showed B. leucurus venom triggers a thromboinflammation scenario where family's proteins of the Serpins, Apolipoproteins, Complement factors and Component subunits, Cathepsins, Kinases, Oxidoreductases, Proteases inhibitors, Proteases, Collagens, Growth factors are related to inflammation, complement and coagulation systems, modulators platelets and neutrophils, lipid and retinoid metabolism, oxidative stress and tissue repair. Our findings set precedents for future studies in the area of early diagnosis and/or treatment of snakebites. Significance: The physiopathological effects that the snake venoms can cause have been investigated through classical and reductionist tools, which allowed, so far, the identification of action mechanisms of individual components associated with specific tissue damage. The currently incomplete limitations of this knowledge must be expanded through new approaches, such as proteomics, which may represent a big leap in understanding the venom-modulated pathological process. The exploration of the complete protein set that suffer modifications by the simultaneous action of multiple toxins, provides a map of the establishment of physiopathological phenotypes, which favors the identification of multiple toxin targets, that may or may not act in synergy, as well as favoring the discovery of biomarkers and therapeutic targets for manifestations that are not neutralized by the antivenom.
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spelling A fingerprint of plasma proteome alteration after local tissue damage induced by Bothrops leucurus snake venom in miceEdemaInflammationMass spectrometrySnake venomThromboinflammationBothrops spp. is responsible for about 70% of snakebites in Brazil, causing a diverse and complex pathophysiological condition. Bothrops leucurus is the main species of medical relevance found in the Atlantic coast in the Brazilian Northeast region. The pathophysiological effects involved B. leucurus snakebite as well as the organism's reaction in response to this envenoming, it has not been explored yet. Thus, edema was induced in mice paw using 1.2, 2.5, and 5.0 μg of B. leucurus venom, the percentage of edema was measured 30 min after injection and the blood plasma was collected and analyzed by shotgun proteomic strategy. We identified 80 common plasma proteins with differential abundance among the experimental groups and we can understand the early aspects of this snake envenomation, regardless of the suggestive severity of an ophidian accident. The results showed B. leucurus venom triggers a thromboinflammation scenario where family's proteins of the Serpins, Apolipoproteins, Complement factors and Component subunits, Cathepsins, Kinases, Oxidoreductases, Proteases inhibitors, Proteases, Collagens, Growth factors are related to inflammation, complement and coagulation systems, modulators platelets and neutrophils, lipid and retinoid metabolism, oxidative stress and tissue repair. Our findings set precedents for future studies in the area of early diagnosis and/or treatment of snakebites. Significance: The physiopathological effects that the snake venoms can cause have been investigated through classical and reductionist tools, which allowed, so far, the identification of action mechanisms of individual components associated with specific tissue damage. The currently incomplete limitations of this knowledge must be expanded through new approaches, such as proteomics, which may represent a big leap in understanding the venom-modulated pathological process. The exploration of the complete protein set that suffer modifications by the simultaneous action of multiple toxins, provides a map of the establishment of physiopathological phenotypes, which favors the identification of multiple toxin targets, that may or may not act in synergy, as well as favoring the discovery of biomarkers and therapeutic targets for manifestations that are not neutralized by the antivenom.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Graduate Program in Tropical Diseases Botucatu Medical School (FMB) São Paulo State University (UNESP)Center of Mathematics Computing Sciences and Cognition Federal University of ABCLaboratory for Structural and Computational Proteomics ICC Oswaldo Cruz Foundation (FIOCRUZ)Drug Research and Development Center Federal University of Ceará (UFC)Center for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP)Biotechnology Institute (IBTEC) São Paulo State University (UNESP)Department of Chemical and Biological Sciences São Paulo State University (UNESP)Department of Physiology and Pharmacology School of Medicine Federal University of Ceará (UFC)Graduate Program in Tropical Diseases Botucatu Medical School (FMB) São Paulo State University (UNESP)Center for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP)Biotechnology Institute (IBTEC) São Paulo State University (UNESP)Department of Chemical and Biological Sciences São Paulo State University (UNESP)CNPq: 437089/2018-5Universidade Estadual Paulista (UNESP)Federal University of ABCOswaldo Cruz Foundation (FIOCRUZ)Federal University of Ceará (UFC)Cavalcante, Joeliton dos Santos [UNESP]de Almeida, Cayo Antônio SoaresClasen, Milan Avilada Silva, Emerson Lucenade Barros, Luciana Curtolo [UNESP]Marinho, Aline DiogoRossini, Bruno Cesar [UNESP]Marino, Celso Luís [UNESP]Carvalho, Paulo CostaJorge, Roberta Jeane Bezerrados Santos, Lucilene Delazari [UNESP]2022-04-29T08:38:03Z2022-04-29T08:38:03Z2022-02-20info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.jprot.2021.104464Journal of Proteomics, v. 253.1876-77371874-3919http://hdl.handle.net/11449/23012510.1016/j.jprot.2021.1044642-s2.0-85121900605Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Proteomicsinfo:eu-repo/semantics/openAccess2024-08-15T15:22:34Zoai:repositorio.unesp.br:11449/230125Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-15T15:22:34Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv A fingerprint of plasma proteome alteration after local tissue damage induced by Bothrops leucurus snake venom in mice
title A fingerprint of plasma proteome alteration after local tissue damage induced by Bothrops leucurus snake venom in mice
spellingShingle A fingerprint of plasma proteome alteration after local tissue damage induced by Bothrops leucurus snake venom in mice
Cavalcante, Joeliton dos Santos [UNESP]
Edema
Inflammation
Mass spectrometry
Snake venom
Thromboinflammation
title_short A fingerprint of plasma proteome alteration after local tissue damage induced by Bothrops leucurus snake venom in mice
title_full A fingerprint of plasma proteome alteration after local tissue damage induced by Bothrops leucurus snake venom in mice
title_fullStr A fingerprint of plasma proteome alteration after local tissue damage induced by Bothrops leucurus snake venom in mice
title_full_unstemmed A fingerprint of plasma proteome alteration after local tissue damage induced by Bothrops leucurus snake venom in mice
title_sort A fingerprint of plasma proteome alteration after local tissue damage induced by Bothrops leucurus snake venom in mice
author Cavalcante, Joeliton dos Santos [UNESP]
author_facet Cavalcante, Joeliton dos Santos [UNESP]
de Almeida, Cayo Antônio Soares
Clasen, Milan Avila
da Silva, Emerson Lucena
de Barros, Luciana Curtolo [UNESP]
Marinho, Aline Diogo
Rossini, Bruno Cesar [UNESP]
Marino, Celso Luís [UNESP]
Carvalho, Paulo Costa
Jorge, Roberta Jeane Bezerra
dos Santos, Lucilene Delazari [UNESP]
author_role author
author2 de Almeida, Cayo Antônio Soares
Clasen, Milan Avila
da Silva, Emerson Lucena
de Barros, Luciana Curtolo [UNESP]
Marinho, Aline Diogo
Rossini, Bruno Cesar [UNESP]
Marino, Celso Luís [UNESP]
Carvalho, Paulo Costa
Jorge, Roberta Jeane Bezerra
dos Santos, Lucilene Delazari [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Federal University of ABC
Oswaldo Cruz Foundation (FIOCRUZ)
Federal University of Ceará (UFC)
dc.contributor.author.fl_str_mv Cavalcante, Joeliton dos Santos [UNESP]
de Almeida, Cayo Antônio Soares
Clasen, Milan Avila
da Silva, Emerson Lucena
de Barros, Luciana Curtolo [UNESP]
Marinho, Aline Diogo
Rossini, Bruno Cesar [UNESP]
Marino, Celso Luís [UNESP]
Carvalho, Paulo Costa
Jorge, Roberta Jeane Bezerra
dos Santos, Lucilene Delazari [UNESP]
dc.subject.por.fl_str_mv Edema
Inflammation
Mass spectrometry
Snake venom
Thromboinflammation
topic Edema
Inflammation
Mass spectrometry
Snake venom
Thromboinflammation
description Bothrops spp. is responsible for about 70% of snakebites in Brazil, causing a diverse and complex pathophysiological condition. Bothrops leucurus is the main species of medical relevance found in the Atlantic coast in the Brazilian Northeast region. The pathophysiological effects involved B. leucurus snakebite as well as the organism's reaction in response to this envenoming, it has not been explored yet. Thus, edema was induced in mice paw using 1.2, 2.5, and 5.0 μg of B. leucurus venom, the percentage of edema was measured 30 min after injection and the blood plasma was collected and analyzed by shotgun proteomic strategy. We identified 80 common plasma proteins with differential abundance among the experimental groups and we can understand the early aspects of this snake envenomation, regardless of the suggestive severity of an ophidian accident. The results showed B. leucurus venom triggers a thromboinflammation scenario where family's proteins of the Serpins, Apolipoproteins, Complement factors and Component subunits, Cathepsins, Kinases, Oxidoreductases, Proteases inhibitors, Proteases, Collagens, Growth factors are related to inflammation, complement and coagulation systems, modulators platelets and neutrophils, lipid and retinoid metabolism, oxidative stress and tissue repair. Our findings set precedents for future studies in the area of early diagnosis and/or treatment of snakebites. Significance: The physiopathological effects that the snake venoms can cause have been investigated through classical and reductionist tools, which allowed, so far, the identification of action mechanisms of individual components associated with specific tissue damage. The currently incomplete limitations of this knowledge must be expanded through new approaches, such as proteomics, which may represent a big leap in understanding the venom-modulated pathological process. The exploration of the complete protein set that suffer modifications by the simultaneous action of multiple toxins, provides a map of the establishment of physiopathological phenotypes, which favors the identification of multiple toxin targets, that may or may not act in synergy, as well as favoring the discovery of biomarkers and therapeutic targets for manifestations that are not neutralized by the antivenom.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-29T08:38:03Z
2022-04-29T08:38:03Z
2022-02-20
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.jprot.2021.104464
Journal of Proteomics, v. 253.
1876-7737
1874-3919
http://hdl.handle.net/11449/230125
10.1016/j.jprot.2021.104464
2-s2.0-85121900605
url http://dx.doi.org/10.1016/j.jprot.2021.104464
http://hdl.handle.net/11449/230125
identifier_str_mv Journal of Proteomics, v. 253.
1876-7737
1874-3919
10.1016/j.jprot.2021.104464
2-s2.0-85121900605
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Proteomics
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128127104188416