A test battery for assessing the ecotoxic effects of textile dyes
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.cbi.2018.06.026 http://hdl.handle.net/11449/171139 |
Resumo: | The textile dyeing industry is one of the main sectors contributing to environmental pollution, due to the generation of large amounts of wastewater loaded with dyes (ca. 2–50% of the initial amount of dyes used in the dye baths is lost), causing severe impacts on human health and the environment. In this context, an ecotoxicity testing battery was used to assess the acute toxicity and genotoxicity of the textile dyes Direct Black 38 (DB38; azo dye) and Reactive Blue 15 (RB15; copper phthalocyanine dye) on different trophic levels. Thus these dyes were tested using the following assays: Filter paper contact test with earthworms (Eisenia foetida); seed germination and root elongation toxicity test (Cucumis sativus, Lactuca sativa and Lycopersicon esculentum); acute immobilization test (Daphnia magna and Artemia salina); and the Comet assay with the rainbow trout gonad-2 cell fish line (RTG-2) and D. magna. Neither phytotoxicity nor significant effects on the survival of E. foetida were observed after exposure to DB38 and RB15. Both dyes were classified as relatively non-toxic to D. magna (LC50 > 100 mg/L), but DB38 was moderately toxic to A. salina with a LC50 of 20.7 mg/L. DB38 and RB15 induced significant effects on the DNA of D. magna but only DB38 caused direct (alkaline comet assay) and oxidative (hOGG1-modified alkaline comet assay) damage to RTG-2 cells in hormetic responses. Therefore, the present results emphasize that a test battery approach of bioassays representing multiple trophic levels is fundamental in predicting the toxicity of textile dyes, aside from providing the information required to define their safe levels for living organisms in the environment. |
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A test battery for assessing the ecotoxic effects of textile dyesThe textile dyeing industry is one of the main sectors contributing to environmental pollution, due to the generation of large amounts of wastewater loaded with dyes (ca. 2–50% of the initial amount of dyes used in the dye baths is lost), causing severe impacts on human health and the environment. In this context, an ecotoxicity testing battery was used to assess the acute toxicity and genotoxicity of the textile dyes Direct Black 38 (DB38; azo dye) and Reactive Blue 15 (RB15; copper phthalocyanine dye) on different trophic levels. Thus these dyes were tested using the following assays: Filter paper contact test with earthworms (Eisenia foetida); seed germination and root elongation toxicity test (Cucumis sativus, Lactuca sativa and Lycopersicon esculentum); acute immobilization test (Daphnia magna and Artemia salina); and the Comet assay with the rainbow trout gonad-2 cell fish line (RTG-2) and D. magna. Neither phytotoxicity nor significant effects on the survival of E. foetida were observed after exposure to DB38 and RB15. Both dyes were classified as relatively non-toxic to D. magna (LC50 > 100 mg/L), but DB38 was moderately toxic to A. salina with a LC50 of 20.7 mg/L. DB38 and RB15 induced significant effects on the DNA of D. magna but only DB38 caused direct (alkaline comet assay) and oxidative (hOGG1-modified alkaline comet assay) damage to RTG-2 cells in hormetic responses. Therefore, the present results emphasize that a test battery approach of bioassays representing multiple trophic levels is fundamental in predicting the toxicity of textile dyes, aside from providing the information required to define their safe levels for living organisms in the environment.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação AraucáriaFundação de Amparo à Pesquisa do Estado de GoiásFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Faculty of Pharmacy (FF) Federal University of Goiás (UFG)Departament of Genetics Federal University of Paraná (UFPR)Unit of Experimental Toxicology and Ecotoxicology (UTOX) Barcelona Science Park (PCB)Association of Biologists of Catalonia (CBC)Faculty of Pharmaceutical Sciences of Ribeirão Preto (FCFRP) University of São Paulo (USP)National Institute for Alternative Technologies of Detection Toxicological Evaluation and Removal of Micropollutants and Radioactives (INCT-DATREM) UNESP Institute of Chemistry, P.O. Box 355National Institute for Alternative Technologies of Detection Toxicological Evaluation and Removal of Micropollutants and Radioactives (INCT-DATREM) UNESP Institute of Chemistry, P.O. Box 355CNPq: 465571/2014-0FAPESP: 2010/01487-2Universidade Federal de Goiás (UFG)Universidade Federal do Paraná (UFPR)Barcelona Science Park (PCB)Association of Biologists of Catalonia (CBC)Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Oliveira, Gisele Augusto Rodrigues de [UNESP]Leme, Daniela Morais [UNESP]de Lapuente, JoaquínBrito, Lara BarrosoPorredón, ConstançaRodrigues, Laís de BritoBrull, NatáliaSerret, Joan TxuBorràs, MiquelDisner, Geonildo RodrigoCestari, Marta MargareteOliveira, Danielle Palma de [UNESP]2018-12-11T16:54:04Z2018-12-11T16:54:04Z2018-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article171-179application/pdfhttp://dx.doi.org/10.1016/j.cbi.2018.06.026Chemico-Biological Interactions, v. 291, p. 171-179.1872-77860009-2797http://hdl.handle.net/11449/17113910.1016/j.cbi.2018.06.0262-s2.0-850490520612-s2.0-85049052061.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengChemico-Biological Interactions1,033info:eu-repo/semantics/openAccess2023-12-22T06:22:38Zoai:repositorio.unesp.br:11449/171139Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:00:55.708801Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
A test battery for assessing the ecotoxic effects of textile dyes |
title |
A test battery for assessing the ecotoxic effects of textile dyes |
spellingShingle |
A test battery for assessing the ecotoxic effects of textile dyes Oliveira, Gisele Augusto Rodrigues de [UNESP] |
title_short |
A test battery for assessing the ecotoxic effects of textile dyes |
title_full |
A test battery for assessing the ecotoxic effects of textile dyes |
title_fullStr |
A test battery for assessing the ecotoxic effects of textile dyes |
title_full_unstemmed |
A test battery for assessing the ecotoxic effects of textile dyes |
title_sort |
A test battery for assessing the ecotoxic effects of textile dyes |
author |
Oliveira, Gisele Augusto Rodrigues de [UNESP] |
author_facet |
Oliveira, Gisele Augusto Rodrigues de [UNESP] Leme, Daniela Morais [UNESP] de Lapuente, Joaquín Brito, Lara Barroso Porredón, Constança Rodrigues, Laís de Brito Brull, Natália Serret, Joan Txu Borràs, Miquel Disner, Geonildo Rodrigo Cestari, Marta Margarete Oliveira, Danielle Palma de [UNESP] |
author_role |
author |
author2 |
Leme, Daniela Morais [UNESP] de Lapuente, Joaquín Brito, Lara Barroso Porredón, Constança Rodrigues, Laís de Brito Brull, Natália Serret, Joan Txu Borràs, Miquel Disner, Geonildo Rodrigo Cestari, Marta Margarete Oliveira, Danielle Palma de [UNESP] |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Goiás (UFG) Universidade Federal do Paraná (UFPR) Barcelona Science Park (PCB) Association of Biologists of Catalonia (CBC) Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Oliveira, Gisele Augusto Rodrigues de [UNESP] Leme, Daniela Morais [UNESP] de Lapuente, Joaquín Brito, Lara Barroso Porredón, Constança Rodrigues, Laís de Brito Brull, Natália Serret, Joan Txu Borràs, Miquel Disner, Geonildo Rodrigo Cestari, Marta Margarete Oliveira, Danielle Palma de [UNESP] |
description |
The textile dyeing industry is one of the main sectors contributing to environmental pollution, due to the generation of large amounts of wastewater loaded with dyes (ca. 2–50% of the initial amount of dyes used in the dye baths is lost), causing severe impacts on human health and the environment. In this context, an ecotoxicity testing battery was used to assess the acute toxicity and genotoxicity of the textile dyes Direct Black 38 (DB38; azo dye) and Reactive Blue 15 (RB15; copper phthalocyanine dye) on different trophic levels. Thus these dyes were tested using the following assays: Filter paper contact test with earthworms (Eisenia foetida); seed germination and root elongation toxicity test (Cucumis sativus, Lactuca sativa and Lycopersicon esculentum); acute immobilization test (Daphnia magna and Artemia salina); and the Comet assay with the rainbow trout gonad-2 cell fish line (RTG-2) and D. magna. Neither phytotoxicity nor significant effects on the survival of E. foetida were observed after exposure to DB38 and RB15. Both dyes were classified as relatively non-toxic to D. magna (LC50 > 100 mg/L), but DB38 was moderately toxic to A. salina with a LC50 of 20.7 mg/L. DB38 and RB15 induced significant effects on the DNA of D. magna but only DB38 caused direct (alkaline comet assay) and oxidative (hOGG1-modified alkaline comet assay) damage to RTG-2 cells in hormetic responses. Therefore, the present results emphasize that a test battery approach of bioassays representing multiple trophic levels is fundamental in predicting the toxicity of textile dyes, aside from providing the information required to define their safe levels for living organisms in the environment. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T16:54:04Z 2018-12-11T16:54:04Z 2018-08-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.cbi.2018.06.026 Chemico-Biological Interactions, v. 291, p. 171-179. 1872-7786 0009-2797 http://hdl.handle.net/11449/171139 10.1016/j.cbi.2018.06.026 2-s2.0-85049052061 2-s2.0-85049052061.pdf |
url |
http://dx.doi.org/10.1016/j.cbi.2018.06.026 http://hdl.handle.net/11449/171139 |
identifier_str_mv |
Chemico-Biological Interactions, v. 291, p. 171-179. 1872-7786 0009-2797 10.1016/j.cbi.2018.06.026 2-s2.0-85049052061 2-s2.0-85049052061.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Chemico-Biological Interactions 1,033 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
171-179 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129273625575424 |